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Q9LFW1

- RGP2_ARATH

UniProt

Q9LFW1 - RGP2_ARATH

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Protein

UDP-arabinopyranose mutase 2

Gene
RGP2, At5g15650, F14F8.30
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

UDP-L-arabinose mutase involved in the biosynthesis of cell wall non-cellulosic polysaccharides. Catalyzes the interconvertion of UDP-L-arabinopyranose (UDP-Arap) and UDP-L-arabinofuranose (UDP-Araf) in vitro. Preferentially catalyzes the formation of UDP-Arap from UDP-Araf. At thermodynamic equilibrium in vitro the ratio of the pyranose form over the furanose form is 95:5. Is not active on other UDP-sugars (UDP-Gal, UDP-Xyl, UDP-Glc, GDP-Man and GDP-Fuc). Functions redundantly with RGP2 and is essential for proper cell walls and pollen development. Probably involved in the formation of the pectocellulosic cell wall layer intine. Is probably active as heteromer in vivo.2 Publications

Catalytic activityi

UDP-beta-L-arabinofuranose = UDP-beta-L-arabinopyranose.

Cofactori

Manganese or Magnesium By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei158 – 1581Required for activity By similarity
Sitei165 – 1651Required for activity By similarity

GO - Molecular functioni

  1. intramolecular transferase activity Source: UniProtKB
  2. protein binding Source: UniProtKB
  3. UDP-arabinopyranose mutase activity Source: TAIR

GO - Biological processi

  1. cellulose biosynthetic process Source: InterPro
  2. plant-type cell wall organization or biogenesis Source: UniProtKB
  3. pollen development Source: UniProtKB
  4. response to cadmium ion Source: TAIR
  5. response to salt stress Source: TAIR
  6. UDP-L-arabinose metabolic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Cell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciARA:AT5G15650-MONOMER.

Protein family/group databases

CAZyiGT75. Glycosyltransferase Family 75.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-arabinopyranose mutase 2 (EC:5.4.99.30)
Alternative name(s):
Reversibly glycosylated polypeptide 2
Short name:
AtRGP2
UDP-L-arabinose mutase 2
Gene namesi
Name:RGP2
Ordered Locus Names:At5g15650
ORF Names:F14F8.30
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 5

Organism-specific databases

TAIRiAT5G15650.

Subcellular locationi

Cytoplasmcytosol. Golgi apparatus
Note: Soluble and membrane-associated.2 Publications

GO - Cellular componenti

  1. cell wall Source: TAIR
  2. cytosol Source: UniProtKB
  3. cytosolic ribosome Source: TAIR
  4. Golgi apparatus Source: UniProtKB
  5. membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Golgi apparatus

Pathology & Biotechi

Disruption phenotypei

No visible phenotype under normal growth condition, but significant reduction in total cell wall arabinose. Rgp1 and rgp2 double mutant is male gametophyte lethal, with an arrest in pollen mitosis (1 Publication). RNAi-mediated knockdown of both RGP1 and RGP2 causes severe developmental defects and strong reduction in total cell wall arabinose (1 Publication).2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 360359UDP-arabinopyranose mutase 2PRO_0000410985Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylvaline1 Publication

Post-translational modificationi

Reversibly glycosylated in vitro by UDP-glucose, UDP-xylose and UDP-galactose, but not UDP-mannose By similarity.

Keywords - PTMi

Acetylation, Glycoprotein

Proteomic databases

PRIDEiQ9LFW1.

Expressioni

Tissue specificityi

Predominantly expressed in shoot and root apical meristems. Expressed in epidermal cells of leaves, inflorescence stems and seed coat. Expressed in pollen.2 Publications

Gene expression databases

GenevestigatoriQ9LFW1.

Interactioni

Subunit structurei

Heteromers with RGP1, RGP4 and RGP5.1 Publication

Protein-protein interaction databases

BioGridi16695. 3 interactions.
IntActiQ9LFW1. 2 interactions.
STRINGi3702.AT5G15650.1-P.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi110 – 1123DXD motif

Domaini

The conserved DXD motif is involved in enzyme activity By similarity.

Sequence similaritiesi

Belongs to the RGP family.

Phylogenomic databases

eggNOGiNOG82578.
HOGENOMiHOG000234443.
InParanoidiQ9LFW1.
KOiK13379.
OMAiQCYIELS.
PhylomeDBiQ9LFW1.

Family and domain databases

InterProiIPR029044. Nucleotide-diphossugar_trans.
IPR004901. RGP_fam.
[Graphical view]
PfamiPF03214. RGP. 1 hit.
[Graphical view]
PIRSFiPIRSF016429. UPTG. 1 hit.
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9LFW1-1 [UniParc]FASTAAdd to Basket

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MVEPANTVGL PVNPTPLLKD ELDIVIPTIR NLDFLEMWRP FLQPYHLIIV    50
QDGDPSKKIH VPEGYDYELY NRNDINRILG PKASCISFKD SACRCFGYMV 100
SKKKYIFTID DDCFVAKDPS GKAVNALEQH IKNLLCPSSP FFFNTLYDPY 150
REGADFVRGY PFSLREGVST AVSHGLWLNI PDYDAPTQLV KPKERNTRYV 200
DAVMTIPKGT LFPMCGMNLA FDRDLIGPAM YFGLMGDGQP IGRYDDMWAG 250
WCIKVICDHL SLGVKTGLPY IYHSKASNPF VNLKKEYKGI FWQEEIIPFF 300
QNAKLSKEAV TVQQCYIELS KMVKEKLSSL DPYFDKLADA MVTWIEAWDE 350
LNPPAASGKA 360
Length:360
Mass (Da):40,890
Last modified:October 1, 2000 - v1
Checksum:i8FE4D48C555F97F1
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti9 – 91G → R in AAM65020. 1 Publication
Sequence conflicti38 – 381W → R in AAM65020. 1 Publication
Sequence conflicti206 – 2061I → N in AAC50001. 1 Publication
Sequence conflicti233 – 2331G → V in AAC50001. 1 Publication
Sequence conflicti360 – 3601A → SLRAV in AAC50001. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF013628 mRNA. Translation: AAC50001.1.
AL391144 Genomic DNA. Translation: CAC01764.1.
CP002688 Genomic DNA. Translation: AED92188.1.
AY039846 mRNA. Translation: AAK63950.1.
AY120691 mRNA. Translation: AAM52234.1.
AY087476 mRNA. Translation: AAM65020.1.
PIRiT51394.
RefSeqiNP_197069.1. NM_121569.2.
UniGeneiAt.24638.

Genome annotation databases

EnsemblPlantsiAT5G15650.1; AT5G15650.1; AT5G15650.
GeneIDi831419.
KEGGiath:AT5G15650.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF013628 mRNA. Translation: AAC50001.1 .
AL391144 Genomic DNA. Translation: CAC01764.1 .
CP002688 Genomic DNA. Translation: AED92188.1 .
AY039846 mRNA. Translation: AAK63950.1 .
AY120691 mRNA. Translation: AAM52234.1 .
AY087476 mRNA. Translation: AAM65020.1 .
PIRi T51394.
RefSeqi NP_197069.1. NM_121569.2.
UniGenei At.24638.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 16695. 3 interactions.
IntActi Q9LFW1. 2 interactions.
STRINGi 3702.AT5G15650.1-P.

Protein family/group databases

CAZyi GT75. Glycosyltransferase Family 75.

Proteomic databases

PRIDEi Q9LFW1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT5G15650.1 ; AT5G15650.1 ; AT5G15650 .
GeneIDi 831419.
KEGGi ath:AT5G15650.

Organism-specific databases

TAIRi AT5G15650.

Phylogenomic databases

eggNOGi NOG82578.
HOGENOMi HOG000234443.
InParanoidi Q9LFW1.
KOi K13379.
OMAi QCYIELS.
PhylomeDBi Q9LFW1.

Enzyme and pathway databases

BioCyci ARA:AT5G15650-MONOMER.

Gene expression databases

Genevestigatori Q9LFW1.

Family and domain databases

InterProi IPR029044. Nucleotide-diphossugar_trans.
IPR004901. RGP_fam.
[Graphical view ]
Pfami PF03214. RGP. 1 hit.
[Graphical view ]
PIRSFi PIRSF016429. UPTG. 1 hit.
SUPFAMi SSF53448. SSF53448. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of AtRGP1. A reversibly autoglycosylated arabidopsis protein implicated in cell wall biosynthesis."
    Delgado I.J., Wang Z., de Rocher A., Keegstra K., Raikhel N.V.
    Plant Physiol. 116:1339-1350(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
    Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.
    , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
    Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  6. "Arabidopsis reversibly glycosylated polypeptides 1 and 2 are essential for pollen development."
    Drakakaki G., Zabotina O., Delgado I., Robert S., Keegstra K., Raikhel N.
    Plant Physiol. 142:1480-1492(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
  7. "The interconversion of UDP-L-arabinopyranose and UDP-L-arabinofuranose is indispensable for plant development in Arabidopsis."
    Rautengarten C., Ebert B., Herter T., Petzold C.J., Ishii T., Mukhopadhyay A., Usadel B., Scheller H.V.
    Plant Cell 23:1373-1390(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, IDENTIFICATION BY MASS SPECTROMETRY, DISRUPTION PHENOTYPE.
  8. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
    Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
    Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT VAL-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiRGP2_ARATH
AccessioniPrimary (citable) accession number: Q9LFW1
Secondary accession number(s): O22428, Q8LB19
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 27, 2011
Last sequence update: October 1, 2000
Last modified: June 11, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi