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Protein

UDP-arabinopyranose mutase 2

Gene

RGP2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

UDP-L-arabinose mutase involved in the biosynthesis of cell wall non-cellulosic polysaccharides. Catalyzes the interconvertion of UDP-L-arabinopyranose (UDP-Arap) and UDP-L-arabinofuranose (UDP-Araf) in vitro. Preferentially catalyzes the formation of UDP-Arap from UDP-Araf. At thermodynamic equilibrium in vitro the ratio of the pyranose form over the furanose form is 95:5. Is not active on other UDP-sugars (UDP-Gal, UDP-Xyl, UDP-Glc, GDP-Man and GDP-Fuc) (PubMed:21478444). Functions redundantly with RGP2 and is essential for proper cell walls and pollen development. Probably involved in the formation of the pectocellulosic cell wall layer intine. Is probably active as heteromer in vivo (PubMed:17071651).2 Publications

Catalytic activityi

UDP-beta-L-arabinofuranose = UDP-beta-L-arabinopyranose.1 Publication

Cofactori

Mn2+By similarity, Mg2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei158Required for activityBy similarity1
Sitei165Required for activityBy similarity1

GO - Molecular functioni

  • intramolecular transferase activity Source: UniProtKB
  • structural constituent of ribosome Source: CAFA
  • UDP-arabinopyranose mutase activity Source: TAIR

GO - Biological processi

  • arabinose biosynthetic process Source: TAIR
  • cell wall organization Source: UniProtKB-KW
  • plant-type cell wall biogenesis Source: TAIR
  • plant-type cell wall organization or biogenesis Source: UniProtKB
  • pollen development Source: UniProtKB
  • response to cadmium ion Source: TAIR
  • response to salt stress Source: TAIR
  • UDP-L-arabinose metabolic process Source: TAIR

Keywordsi

Molecular functionIsomerase
Biological processCell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciARA:AT5G15650-MONOMER

Protein family/group databases

CAZyiGT75 Glycosyltransferase Family 75

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-arabinopyranose mutase 2Curated (EC:5.4.99.301 Publication)
Alternative name(s):
Reversibly glycosylated polypeptide 21 Publication
Short name:
AtRGP21 Publication
UDP-L-arabinose mutase 2Curated
Gene namesi
Name:RGP21 Publication
Ordered Locus Names:At5g15650Imported
ORF Names:F14F8_30Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G15650
TAIRilocus:2143171 AT5G15650

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Golgi apparatus

Pathology & Biotechi

Disruption phenotypei

No visible phenotype under normal growth condition, but significant reduction in total cell wall arabinose. Rgp1 and rgp2 double mutant is male gametophyte lethal, with an arrest in pollen mitosis (PubMed:17071651). RNAi-mediated knockdown of both RGP1 and RGP2 causes severe developmental defects and strong reduction in total cell wall arabinose (PubMed:21478444).2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00004109852 – 360UDP-arabinopyranose mutase 2Add BLAST359

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylvalineCombined sources1
Glycosylationi158N-linked (Glc...) arginineBy similarity1

Post-translational modificationi

Reversibly glycosylated in vitro by UDP-glucose, UDP-xylose and UDP-galactose, but not UDP-mannose.By similarity

Keywords - PTMi

Acetylation, Glycoprotein

Proteomic databases

PaxDbiQ9LFW1
PRIDEiQ9LFW1
ProMEXiQ9LFW1

PTM databases

iPTMnetiQ9LFW1

Expressioni

Tissue specificityi

Predominantly expressed in shoot and root apical meristems. Expressed in epidermal cells of leaves, inflorescence stems and seed coat. Expressed in pollen.2 Publications

Gene expression databases

ExpressionAtlasiQ9LFW1 baseline and differential
GenevisibleiQ9LFW1 AT

Interactioni

Subunit structurei

Heteromers with RGP1, RGP4 and RGP5.1 Publication

Protein-protein interaction databases

BioGridi16695, 3 interactors
IntActiQ9LFW1, 2 interactors
STRINGi3702.AT5G15650.1

Structurei

3D structure databases

SMRiQ9LFW1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi110 – 112DXD motifBy similarity3

Domaini

The conserved DXD motif is involved in enzyme activity.By similarity

Sequence similaritiesi

Belongs to the RGP family.Curated

Phylogenomic databases

eggNOGiENOG410IHTP Eukaryota
ENOG411144T LUCA
HOGENOMiHOG000234443
InParanoidiQ9LFW1
KOiK13379
OMAiVTWIDAW
OrthoDBiEOG09360C9G
PhylomeDBiQ9LFW1

Family and domain databases

InterProiView protein in InterPro
IPR029044 Nucleotide-diphossugar_trans
IPR004901 RGP
IPR037595 RGP_fam
PANTHERiPTHR31682 PTHR31682, 1 hit
PfamiView protein in Pfam
PF03214 RGP, 1 hit
PIRSFiPIRSF016429 UPTG, 1 hit
SUPFAMiSSF53448 SSF53448, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9LFW1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVEPANTVGL PVNPTPLLKD ELDIVIPTIR NLDFLEMWRP FLQPYHLIIV
60 70 80 90 100
QDGDPSKKIH VPEGYDYELY NRNDINRILG PKASCISFKD SACRCFGYMV
110 120 130 140 150
SKKKYIFTID DDCFVAKDPS GKAVNALEQH IKNLLCPSSP FFFNTLYDPY
160 170 180 190 200
REGADFVRGY PFSLREGVST AVSHGLWLNI PDYDAPTQLV KPKERNTRYV
210 220 230 240 250
DAVMTIPKGT LFPMCGMNLA FDRDLIGPAM YFGLMGDGQP IGRYDDMWAG
260 270 280 290 300
WCIKVICDHL SLGVKTGLPY IYHSKASNPF VNLKKEYKGI FWQEEIIPFF
310 320 330 340 350
QNAKLSKEAV TVQQCYIELS KMVKEKLSSL DPYFDKLADA MVTWIEAWDE
360
LNPPAASGKA
Length:360
Mass (Da):40,890
Last modified:October 1, 2000 - v1
Checksum:i8FE4D48C555F97F1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti9G → R in AAM65020 (Ref. 5) Curated1
Sequence conflicti38W → R in AAM65020 (Ref. 5) Curated1
Sequence conflicti206I → N in AAC50001 (PubMed:9536051).Curated1
Sequence conflicti233G → V in AAC50001 (PubMed:9536051).Curated1
Sequence conflicti360A → SLRAV in AAC50001 (PubMed:9536051).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF013628 mRNA Translation: AAC50001.1
AL391144 Genomic DNA Translation: CAC01764.1
CP002688 Genomic DNA Translation: AED92188.1
AY039846 mRNA Translation: AAK63950.1
AY120691 mRNA Translation: AAM52234.1
AY087476 mRNA Translation: AAM65020.1
PIRiT51394
RefSeqiNP_197069.1, NM_121569.3
UniGeneiAt.24638

Genome annotation databases

EnsemblPlantsiAT5G15650.1; AT5G15650.1; AT5G15650
GeneIDi831419
GrameneiAT5G15650.1; AT5G15650.1; AT5G15650
KEGGiath:AT5G15650

Similar proteinsi

Entry informationi

Entry nameiRGP2_ARATH
AccessioniPrimary (citable) accession number: Q9LFW1
Secondary accession number(s): O22428, Q8LB19
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 27, 2011
Last sequence update: October 1, 2000
Last modified: April 25, 2018
This is version 115 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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