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Q9LFW1 (RGP2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
UDP-arabinopyranose mutase 2

EC=5.4.99.30
Alternative name(s):
Reversibly glycosylated polypeptide 2
Short name=AtRGP2
UDP-L-arabinose mutase 2
Gene names
Name:RGP2
Ordered Locus Names:At5g15650
ORF Names:F14F8.30
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length360 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

UDP-L-arabinose mutase involved in the biosynthesis of cell wall non-cellulosic polysaccharides. Catalyzes the interconvertion of UDP-L-arabinopyranose (UDP-Arap) and UDP-L-arabinofuranose (UDP-Araf) in vitro. Preferentially catalyzes the formation of UDP-Arap from UDP-Araf. At thermodynamic equilibrium in vitro the ratio of the pyranose form over the furanose form is 95:5. Is not active on other UDP-sugars (UDP-Gal, UDP-Xyl, UDP-Glc, GDP-Man and GDP-Fuc). Functions redundantly with RGP2 and is essential for proper cell walls and pollen development. Probably involved in the formation of the pectocellulosic cell wall layer intine. Is probably active as heteromer in vivo. Ref.6 Ref.7

Catalytic activity

UDP-beta-L-arabinofuranose = UDP-beta-L-arabinopyranose.

Cofactor

Manganese or Magnesium By similarity.

Subunit structure

Heteromers with RGP1, RGP4 and RGP5. Ref.6

Subcellular location

Cytoplasmcytosol. Golgi apparatus. Note: Soluble and membrane-associated. Ref.6 Ref.7

Tissue specificity

Predominantly expressed in shoot and root apical meristems. Expressed in epidermal cells of leaves, inflorescence stems and seed coat. Expressed in pollen. Ref.6 Ref.7

Domain

The conserved DXD motif is involved in enzyme activity By similarity.

Post-translational modification

Reversibly glycosylated in vitro by UDP-glucose, UDP-xylose and UDP-galactose, but not UDP-mannose By similarity.

Disruption phenotype

No visible phenotype under normal growth condition, but significant reduction in total cell wall arabinose. Rgp1 and rgp2 double mutant is male gametophyte lethal, with an arrest in pollen mitosis (Ref.6). RNAi-mediated knockdown of both RGP1 and RGP2 causes severe developmental defects and strong reduction in total cell wall arabinose (Ref.7). Ref.6 Ref.7

Sequence similarities

Belongs to the RGP family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.8
Chain2 – 360359UDP-arabinopyranose mutase 2
PRO_0000410985

Regions

Motif110 – 1123DXD motif

Sites

Site1581Required for activity By similarity
Site1651Required for activity By similarity

Amino acid modifications

Modified residue21N-acetylvaline Ref.8

Experimental info

Sequence conflict91G → R in AAM65020. Ref.5
Sequence conflict381W → R in AAM65020. Ref.5
Sequence conflict2061I → N in AAC50001. Ref.1
Sequence conflict2331G → V in AAC50001. Ref.1
Sequence conflict3601A → SLRAV in AAC50001. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q9LFW1 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 8FE4D48C555F97F1

FASTA36040,890
        10         20         30         40         50         60 
MVEPANTVGL PVNPTPLLKD ELDIVIPTIR NLDFLEMWRP FLQPYHLIIV QDGDPSKKIH 

        70         80         90        100        110        120 
VPEGYDYELY NRNDINRILG PKASCISFKD SACRCFGYMV SKKKYIFTID DDCFVAKDPS 

       130        140        150        160        170        180 
GKAVNALEQH IKNLLCPSSP FFFNTLYDPY REGADFVRGY PFSLREGVST AVSHGLWLNI 

       190        200        210        220        230        240 
PDYDAPTQLV KPKERNTRYV DAVMTIPKGT LFPMCGMNLA FDRDLIGPAM YFGLMGDGQP 

       250        260        270        280        290        300 
IGRYDDMWAG WCIKVICDHL SLGVKTGLPY IYHSKASNPF VNLKKEYKGI FWQEEIIPFF 

       310        320        330        340        350        360 
QNAKLSKEAV TVQQCYIELS KMVKEKLSSL DPYFDKLADA MVTWIEAWDE LNPPAASGKA 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and characterization of AtRGP1. A reversibly autoglycosylated arabidopsis protein implicated in cell wall biosynthesis."
Delgado I.J., Wang Z., de Rocher A., Keegstra K., Raikhel N.V.
Plant Physiol. 116:1339-1350(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K. expand/collapse author list , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"Full-length cDNA from Arabidopsis thaliana."
Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]"Arabidopsis reversibly glycosylated polypeptides 1 and 2 are essential for pollen development."
Drakakaki G., Zabotina O., Delgado I., Robert S., Keegstra K., Raikhel N.
Plant Physiol. 142:1480-1492(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
[7]"The interconversion of UDP-L-arabinopyranose and UDP-L-arabinofuranose is indispensable for plant development in Arabidopsis."
Rautengarten C., Ebert B., Herter T., Petzold C.J., Ishii T., Mukhopadhyay A., Usadel B., Scheller H.V.
Plant Cell 23:1373-1390(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, IDENTIFICATION BY MASS SPECTROMETRY, DISRUPTION PHENOTYPE.
[8]"Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT VAL-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF013628 mRNA. Translation: AAC50001.1.
AL391144 Genomic DNA. Translation: CAC01764.1.
CP002688 Genomic DNA. Translation: AED92188.1.
AY039846 mRNA. Translation: AAK63950.1.
AY120691 mRNA. Translation: AAM52234.1.
AY087476 mRNA. Translation: AAM65020.1.
PIRT51394.
RefSeqNP_197069.1. NM_121569.2.
UniGeneAt.24638.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid16695. 3 interactions.
IntActQ9LFW1. 2 interactions.
STRING3702.AT5G15650.1-P.

Protein family/group databases

CAZyGT75. Glycosyltransferase Family 75.

Proteomic databases

PRIDEQ9LFW1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G15650.1; AT5G15650.1; AT5G15650.
GeneID831419.
KEGGath:AT5G15650.

Organism-specific databases

TAIRAT5G15650.

Phylogenomic databases

eggNOGNOG82578.
HOGENOMHOG000234443.
InParanoidQ9LFW1.
KOK13379.
OMAQCYIELS.
PhylomeDBQ9LFW1.

Enzyme and pathway databases

BioCycARA:AT5G15650-MONOMER.

Gene expression databases

GenevestigatorQ9LFW1.

Family and domain databases

InterProIPR029044. Nucleotide-diphossugar_trans.
IPR004901. RGP_fam.
[Graphical view]
PfamPF03214. RGP. 1 hit.
[Graphical view]
PIRSFPIRSF016429. UPTG. 1 hit.
SUPFAMSSF53448. SSF53448. 1 hit.
ProtoNetSearch...

Entry information

Entry nameRGP2_ARATH
AccessionPrimary (citable) accession number: Q9LFW1
Secondary accession number(s): O22428, Q8LB19
Entry history
Integrated into UniProtKB/Swiss-Prot: July 27, 2011
Last sequence update: October 1, 2000
Last modified: June 11, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names