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Q9LFU1

- ASNS3_ARATH

UniProt

Q9LFU1 - ASNS3_ARATH

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Protein

Asparagine synthetase [glutamine-hydrolyzing] 3

Gene
ASN3, At5g10240, F18D22.10, T31P16.230
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Essential for nitrogen assimilation, distribution and remobilization within the plant via the phloem By similarity.

Catalytic activityi

ATP + L-aspartate + L-glutamine + H2O = AMP + diphosphate + L-asparagine + L-glutamate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei2 – 21For GATase activity By similarity
Binding sitei98 – 981Glutamine By similarity
Binding sitei231 – 2311ATP; via carbonyl oxygen By similarity
Binding sitei267 – 2671ATP; via amide nitrogen and carbonyl oxygen By similarity
Sitei343 – 3431Important for beta-aspartyl-AMP intermediate formation By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi341 – 3422ATP By similarity

GO - Molecular functioni

  1. asparagine synthase (glutamine-hydrolyzing) activity Source: UniProtKB-EC
  2. aspartate-ammonia ligase activity Source: TAIR
  3. ATP binding Source: UniProtKB-KW

GO - Biological processi

  1. asparagine biosynthetic process Source: TAIR
  2. glutamine metabolic process Source: UniProtKB-KW
  3. L-asparagine biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Asparagine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:GQT-424-MONOMER.
UniPathwayiUPA00134.

Protein family/group databases

MEROPSiC44.976.

Names & Taxonomyi

Protein namesi
Recommended name:
Asparagine synthetase [glutamine-hydrolyzing] 3 (EC:6.3.5.4)
Alternative name(s):
Glutamine-dependent asparagine synthetase 3
Gene namesi
Name:ASN3
Ordered Locus Names:At5g10240
ORF Names:F18D22.10, T31P16.230
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 5

Organism-specific databases

TAIRiAT5G10240.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: TAIR
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 578577Asparagine synthetase [glutamine-hydrolyzing] 3PRO_0000420841Add
BLAST

Proteomic databases

PaxDbiQ9LFU1.
PRIDEiQ9LFU1.

Expressioni

Gene expression databases

GenevestigatoriQ9LFU1.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT5G10240.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ9LFU1.
SMRiQ9LFU1. Positions 18-517.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 185184Glutamine amidotransferase type-2Add
BLAST
Domaini210 – 450241Asparagine synthetaseAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni50 – 545Glutamine binding By similarity
Regioni75 – 773Glutamine binding By similarity

Sequence similaritiesi

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiCOG0367.
HOGENOMiHOG000027493.
InParanoidiQ9LFU1.
KOiK01953.
OMAiEKSEAAC.
PhylomeDBiQ9LFU1.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.60.20.10. 1 hit.
InterProiIPR006426. Asn_synth_AEB.
IPR001962. Asn_synthase.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00733. Asn_synthase. 1 hit.
PF13537. GATase_7. 1 hit.
[Graphical view]
PIRSFiPIRSF001589. Asn_synthetase_glu-h. 1 hit.
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01536. asn_synth_AEB. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. Align

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q9LFU1-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MCGILAVLGC VDNSQAKRSR IIELSRRLRH RGPDWSGLHC YEDCYLAHER    50
LAIVDPTSGD QPLYNEDKTI AVTVNGEIYN HKALRENLKS HQFRTGSDCE 100
VIAHLYEEHG EEFVDMLDGM FAFVLLDTRD KSFIAARDAI GITPLYIGWG 150
LDGSVWFASE MKALSDDCEQ FMCFPPGHIY SSKQGGLRRW YNPPWFSEVV 200
PSTPYDPLVV RNTFEKAVIK RLMTDVPFGV LLSGGLDSSL VASVALRHLE 250
KSEAACQWGS KLHTFCIGLK GSPDLKAGRE VADYLGTRHH ELHFTVQDGI 300
DAIEEVIYHV ETYDVTTIRA STPMFLMSRK IKSLGVKMVL SGEGSDEIFG 350
GYLYFHKAPN KKEFHEETCR KIKALHQYDC LRANKSTSAW GVEARVPFLD 400
KEFINVAMSI DPEWKMIRPD LGRIEKWVLR NAFDDEKNPY LPKHILYRQK 450
EQFSDGVGYS WIDGLKDHAN KHVSETMLMN ASFVFPDNTP LTKEAYYYRT 500
IFEKFFPKSA ARATVPGGPS VACSTAKAVE WDAAWSQNLD PSGRAALGVH 550
VSAYGEDKTE DSRPEKLQKL AEKTPAIV 578
Length:578
Mass (Da):65,227
Last modified:October 1, 2000 - v1
Checksum:i2CF8FE216BBDD83E
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti83 – 831A → D in AAC72836. 1 Publication
Sequence conflicti228 – 2281F → L in AAC72836. 1 Publication
Sequence conflicti475 – 4751E → D in AAC72836. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF095452 mRNA. Translation: AAC72836.1.
AL356332 Genomic DNA. Translation: CAB92065.1.
AL360334 Genomic DNA. Translation: CAB96680.1.
CP002688 Genomic DNA. Translation: AED91511.1.
BT003929 mRNA. Translation: AAO41976.1.
BT005014 mRNA. Translation: AAO50547.1.
PIRiT50812.
T51888.
RefSeqiNP_196586.1. NM_121062.4. [Q9LFU1-1]
UniGeneiAt.1637.

Genome annotation databases

EnsemblPlantsiAT5G10240.1; AT5G10240.1; AT5G10240. [Q9LFU1-1]
GeneIDi830888.
KEGGiath:AT5G10240.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF095452 mRNA. Translation: AAC72836.1 .
AL356332 Genomic DNA. Translation: CAB92065.1 .
AL360334 Genomic DNA. Translation: CAB96680.1 .
CP002688 Genomic DNA. Translation: AED91511.1 .
BT003929 mRNA. Translation: AAO41976.1 .
BT005014 mRNA. Translation: AAO50547.1 .
PIRi T50812.
T51888.
RefSeqi NP_196586.1. NM_121062.4. [Q9LFU1-1 ]
UniGenei At.1637.

3D structure databases

ProteinModelPortali Q9LFU1.
SMRi Q9LFU1. Positions 18-517.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 3702.AT5G10240.1-P.

Protein family/group databases

MEROPSi C44.976.

Proteomic databases

PaxDbi Q9LFU1.
PRIDEi Q9LFU1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT5G10240.1 ; AT5G10240.1 ; AT5G10240 . [Q9LFU1-1 ]
GeneIDi 830888.
KEGGi ath:AT5G10240.

Organism-specific databases

TAIRi AT5G10240.

Phylogenomic databases

eggNOGi COG0367.
HOGENOMi HOG000027493.
InParanoidi Q9LFU1.
KOi K01953.
OMAi EKSEAAC.
PhylomeDBi Q9LFU1.

Enzyme and pathway databases

UniPathwayi UPA00134 .
BioCyci ARA:GQT-424-MONOMER.

Gene expression databases

Genevestigatori Q9LFU1.

Family and domain databases

Gene3Di 3.40.50.620. 1 hit.
3.60.20.10. 1 hit.
InterProi IPR006426. Asn_synth_AEB.
IPR001962. Asn_synthase.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view ]
Pfami PF00733. Asn_synthase. 1 hit.
PF13537. GATase_7. 1 hit.
[Graphical view ]
PIRSFi PIRSF001589. Asn_synthetase_glu-h. 1 hit.
SUPFAMi SSF56235. SSF56235. 1 hit.
TIGRFAMsi TIGR01536. asn_synth_AEB. 1 hit.
PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Reciprocal regulation of distinct asparagine synthetase genes by light and metabolites in Arabidopsis thaliana."
    Lam H.M., Hsieh M.H., Coruzzi G.
    Plant J. 16:345-353(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
    Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.
    , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
    Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.

Entry informationi

Entry nameiASNS3_ARATH
AccessioniPrimary (citable) accession number: Q9LFU1
Secondary accession number(s): Q9LEA1, Q9ZST7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 9, 2013
Last sequence update: October 1, 2000
Last modified: June 11, 2014
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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