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Protein

Asparagine synthetase [glutamine-hydrolyzing] 3

Gene

ASN3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Essential for nitrogen assimilation, distribution and remobilization within the plant via the phloem.By similarity

Catalytic activityi

ATP + L-aspartate + L-glutamine + H2O = AMP + diphosphate + L-asparagine + L-glutamate.

Pathwayi: L-asparagine biosynthesis

This protein is involved in the pathway L-asparagine biosynthesis, which is part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the pathway L-asparagine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei2For GATase activityBy similarity1
Binding sitei98GlutamineBy similarity1
Binding sitei231ATP; via carbonyl oxygenBy similarity1
Binding sitei267ATP; via amide nitrogen and carbonyl oxygenBy similarity1
Sitei343Important for beta-aspartyl-AMP intermediate formationBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi341 – 342ATPBy similarity2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processAmino-acid biosynthesis, Asparagine biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-ATH-70614 Amino acid synthesis and interconversion (transamination)
UniPathwayiUPA00134

Names & Taxonomyi

Protein namesi
Recommended name:
Asparagine synthetase [glutamine-hydrolyzing] 3 (EC:6.3.5.4)
Alternative name(s):
Glutamine-dependent asparagine synthetase 3
Gene namesi
Name:ASN3
Ordered Locus Names:At5g10240
ORF Names:F18D22.10, T31P16.230
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G10240
TAIRilocus:2145377 AT5G10240

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00004208412 – 578Asparagine synthetase [glutamine-hydrolyzing] 3Add BLAST577

Proteomic databases

PaxDbiQ9LFU1
PRIDEiQ9LFU1
ProMEXiQ9LFU1

Expressioni

Gene expression databases

ExpressionAtlasiQ9LFU1 baseline and differential
GenevisibleiQ9LFU1 AT

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi16166, 1 interactor
STRINGi3702.AT5G10240.1

Structurei

3D structure databases

ProteinModelPortaliQ9LFU1
SMRiQ9LFU1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 185Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd BLAST184
Domaini210 – 450Asparagine synthetaseAdd BLAST241

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni50 – 54Glutamine bindingBy similarity5
Regioni75 – 77Glutamine bindingBy similarity3

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiKOG0571 Eukaryota
COG0367 LUCA
HOGENOMiHOG000027493
InParanoidiQ9LFU1
KOiK01953
OMAiYFHAAPD
OrthoDBiEOG093604QK
PhylomeDBiQ9LFU1

Family and domain databases

CDDicd01991 Asn_Synthase_B_C, 1 hit
cd00712 AsnB, 1 hit
Gene3Di3.40.50.620, 1 hit
3.60.20.10, 1 hit
InterProiView protein in InterPro
IPR006426 Asn_synth_AEB
IPR001962 Asn_synthase
IPR033738 AsnB_N
IPR017932 GATase_2_dom
IPR029055 Ntn_hydrolases_N
IPR014729 Rossmann-like_a/b/a_fold
PfamiView protein in Pfam
PF00733 Asn_synthase, 1 hit
PF13537 GATase_7, 1 hit
PIRSFiPIRSF001589 Asn_synthetase_glu-h, 1 hit
SUPFAMiSSF56235 SSF56235, 1 hit
TIGRFAMsiTIGR01536 asn_synth_AEB, 1 hit
PROSITEiView protein in PROSITE
PS51278 GATASE_TYPE_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q9LFU1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MCGILAVLGC VDNSQAKRSR IIELSRRLRH RGPDWSGLHC YEDCYLAHER
60 70 80 90 100
LAIVDPTSGD QPLYNEDKTI AVTVNGEIYN HKALRENLKS HQFRTGSDCE
110 120 130 140 150
VIAHLYEEHG EEFVDMLDGM FAFVLLDTRD KSFIAARDAI GITPLYIGWG
160 170 180 190 200
LDGSVWFASE MKALSDDCEQ FMCFPPGHIY SSKQGGLRRW YNPPWFSEVV
210 220 230 240 250
PSTPYDPLVV RNTFEKAVIK RLMTDVPFGV LLSGGLDSSL VASVALRHLE
260 270 280 290 300
KSEAACQWGS KLHTFCIGLK GSPDLKAGRE VADYLGTRHH ELHFTVQDGI
310 320 330 340 350
DAIEEVIYHV ETYDVTTIRA STPMFLMSRK IKSLGVKMVL SGEGSDEIFG
360 370 380 390 400
GYLYFHKAPN KKEFHEETCR KIKALHQYDC LRANKSTSAW GVEARVPFLD
410 420 430 440 450
KEFINVAMSI DPEWKMIRPD LGRIEKWVLR NAFDDEKNPY LPKHILYRQK
460 470 480 490 500
EQFSDGVGYS WIDGLKDHAN KHVSETMLMN ASFVFPDNTP LTKEAYYYRT
510 520 530 540 550
IFEKFFPKSA ARATVPGGPS VACSTAKAVE WDAAWSQNLD PSGRAALGVH
560 570
VSAYGEDKTE DSRPEKLQKL AEKTPAIV
Length:578
Mass (Da):65,227
Last modified:October 1, 2000 - v1
Checksum:i2CF8FE216BBDD83E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti83A → D in AAC72836 (PubMed:9881155).Curated1
Sequence conflicti228F → L in AAC72836 (PubMed:9881155).Curated1
Sequence conflicti475E → D in AAC72836 (PubMed:9881155).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF095452 mRNA Translation: AAC72836.1
AL356332 Genomic DNA Translation: CAB92065.1
AL360334 Genomic DNA Translation: CAB96680.1
CP002688 Genomic DNA Translation: AED91511.1
BT003929 mRNA Translation: AAO41976.1
BT005014 mRNA Translation: AAO50547.1
PIRiT50812
T51888
RefSeqiNP_196586.1, NM_121062.5 [Q9LFU1-1]
UniGeneiAt.1637

Genome annotation databases

EnsemblPlantsiAT5G10240.1; AT5G10240.1; AT5G10240 [Q9LFU1-1]
GeneIDi830888
GrameneiAT5G10240.1; AT5G10240.1; AT5G10240 [Q9LFU1-1]
KEGGiath:AT5G10240

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiASNS3_ARATH
AccessioniPrimary (citable) accession number: Q9LFU1
Secondary accession number(s): Q9LEA1, Q9ZST7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 9, 2013
Last sequence update: October 1, 2000
Last modified: May 23, 2018
This is version 116 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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