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Q9LFT6 (HNL_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Alpha-hydroxynitrile lyase

Short name=AtHNL
EC=4.1.2.10
Alternative name(s):
(R)-hydroxynitrile lyase
(R)-oxynitrilase
Methylesterase 5
Short name=AtMES5
Gene names
Name:HNL
Synonyms:MES5
Ordered Locus Names:At5g10300
ORF Names:F18D22_70
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length258 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in cyanogenesis, the release of HCN from injured tissues By similarity. Displays R-selective hydroxynitrile lyase activity. Also accepts nitromethane (MeNO2) as a donor in a reaction with aromatic aldehydes to yield (R)-beta-nitro alcohols. Ref.5 Ref.6 Ref.7 Ref.8

Catalytic activity

(R)-mandelonitrile = cyanide + benzaldehyde. Ref.8

Subunit structure

Homodimer By similarity.

Sequence similarities

Belongs to the AB hydrolase superfamily. Hydroxynitrile lyase family.

Caution

Was originally thought to belong to the methylesterase (MES) family (Ref.6).

Biophysicochemical properties

Kinetic parameters:

KM=1.4 mM for mandelonitrile Ref.6

Binary interactions

With

Entry

#Exp.

IntAct

Notes

MES9O231713EBI-4453194,EBI-4446268

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 258258Alpha-hydroxynitrile lyase
PRO_0000418174

Sites

Active site811
Active site2081
Active site2361

Experimental info

Mutagenesis811S → A: Loss of activity. Ref.8
Mutagenesis2081D → N: Loss of activity. Ref.8
Mutagenesis2361H → F: Loss of activity. Ref.8
Sequence conflict701P → Q in AAL25532. Ref.3
Sequence conflict751V → G in AAL25532. Ref.3
Sequence conflict1771G → R in AAK76689. Ref.3

Secondary structure

................................................. 258
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q9LFT6 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 0CFA965320F1FD2E

FASTA25829,217
        10         20         30         40         50         60 
MERKHHFVLV HNAYHGAWIW YKLKPLLESA GHRVTAVELA ASGIDPRPIQ AVETVDEYSK 

        70         80         90        100        110        120 
PLIETLKSLP ENEEVILVGF SFGGINIALA ADIFPAKIKV LVFLNAFLPD TTHVPSHVLD 

       130        140        150        160        170        180 
KYMEMPGGLG DCEFSSHETR NGTMSLLKMG PKFMKARLYQ NCPIEDYELA KMLHRQGSFF 

       190        200        210        220        230        240 
TEDLSKKEKF SEEGYGSVQR VYVMSSEDKA IPCDFIRWMI DNFNVSKVYE IDGGDHMVML 

       250 
SKPQKLFDSL SAIATDYM 

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K. expand/collapse author list , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. expand/collapse author list , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"Inactive methyl indole-3-acetic acid ester can be hydrolyzed and activated by several esterases belonging to the AtMES esterase family of Arabidopsis."
Yang Y., Xu R., Ma C.J., Vlot A.C., Klessig D.F., Pichersky E.
Plant Physiol. 147:1034-1045(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, FUNCTION.
[6]"Uneven twins: comparison of two enantiocomplementary hydroxynitrile lyases with alpha/beta-hydrolase fold."
Guterl J.K., Andexer J.N., Sehl T., von Langermann J., Frindi-Wosch I., Rosenkranz T., Fitter J., Gruber K., Kragl U., Eggert T., Pohl M.
J. Biotechnol. 141:166-173(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
[7]"Synthesis of (R)-?-nitro alcohols catalyzed by R-selective hydroxynitrile lyase from Arabidopsis thaliana in the aqueous-organic biphasic system."
Fuhshuku K., Asano Y.
J. Biotechnol. 153:153-159(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[8]"An R-selective hydroxynitrile lyase from Arabidopsis thaliana with an alpha/beta-hydrolase fold."
Andexer J., von Langermann J., Mell A., Bocola M., Kragl U., Eggert T., Pohl M.
Angew. Chem. Int. Ed. Engl. 46:8679-8681(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.50 ANGSTROMS), FUNCTION, CATALYTIC ACTIVITY, MUTAGENESIS OF SER-81; ASP-208 AND HIS-236.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL360334 Genomic DNA. Translation: CAB96686.1.
CP002688 Genomic DNA. Translation: AED91518.1.
AY046015 mRNA. Translation: AAK76689.1.
AY058115 mRNA. Translation: AAL25532.1.
AY093714 mRNA. Translation: AAM10338.1.
AY142490 mRNA. Translation: AAN13041.1.
AK226255 mRNA. Translation: BAE98416.1.
PIRT50818.
RefSeqNP_196592.1. NM_121068.3.
UniGeneAt.19934.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3DQZX-ray2.50A/B/C/D1-258[»]
ProteinModelPortalQ9LFT6.
SMRQ9LFT6. Positions 3-258.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ9LFT6. 1 interaction.
STRING3702.AT5G10300.1-P.

Proteomic databases

PRIDEQ9LFT6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G10300.1; AT5G10300.1; AT5G10300.
GeneID830894.
KEGGath:AT5G10300.

Organism-specific databases

TAIRAT5G10300.

Phylogenomic databases

eggNOGNOG252413.
HOGENOMHOG000133452.
InParanoidQ9LFT6.
KOK08233.
OMASHETRNG.
PhylomeDBQ9LFT6.

Enzyme and pathway databases

BioCycARA:AT5G10300-MONOMER.

Gene expression databases

GenevestigatorQ9LFT6.

Family and domain databases

Gene3D3.40.50.1820. 1 hit.
InterProIPR029058. AB_hydrolase.
[Graphical view]
SUPFAMSSF53474. SSF53474. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceQ9LFT6.

Entry information

Entry nameHNL_ARATH
AccessionPrimary (citable) accession number: Q9LFT6
Secondary accession number(s): Q93Z57, Q94AI5
Entry history
Integrated into UniProtKB/Swiss-Prot: July 11, 2012
Last sequence update: October 1, 2000
Last modified: June 11, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names