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Protein

E2F transcription factor-like E2FD

Gene

E2FD

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Inhibitor of E2F-dependent regulation of gene expression. Binds specifically the E2 recognition site as a monomer without interacting with DP proteins. May be up-regulating E2FA and down-regulating repressors of cell cycle progression. Promotes cell proliferation and represses cell elongation. Regulated by proteolysis via a ubiquitin-proteasome pathway.3 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi13 – 78Add BLAST66
DNA bindingi138 – 217Add BLAST80

GO - Molecular functioni

  • DNA binding Source: TAIR
  • transcription factor activity, sequence-specific DNA binding Source: TAIR

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • positive regulation of cell proliferation Source: UniProtKB
  • response to auxin Source: UniProtKB
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor

Keywords - Biological processi

Cell cycle, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
E2F transcription factor-like E2FD
Alternative name(s):
DP-E2F-like protein 2
E2F-like repressor E2L1
Gene namesi
Name:E2FD
Synonyms:DEL2, E2L1, ELP3
Ordered Locus Names:At5g14960
ORF Names:F2G14.80
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G14960.

Subcellular locationi

  • Nucleus 1 Publication

GO - Cellular componenti

  • nucleus Source: TAIR
  • transcription factor complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Increased root length and smaller cotyledons. Reduced root meristematic zone but longer cells in the differentiation zone.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004062921 – 359E2F transcription factor-like E2FDAdd BLAST359

Proteomic databases

PaxDbiQ9LFQ9.
PRIDEiQ9LFQ9.

Expressioni

Tissue specificityi

Preferentially expressed in proliferating tissues. Highly expressed in young stalk and young flowers. Lower expression in young leaves and mature flowers. Detected in cotyledonary vascular tissues, the shoot apical meristem, the base of trichomes, the fully developed stomata, the central root cylinder and in the columella of lateral roots but not in the primary root tips or in the leaf epidermal cells.2 Publications

Developmental stagei

Expressed in a cell cycle-dependent manner. Not detected at the G1/S transition, but increases during the progression into S phase and peaks after the passage into G2.1 Publication

Inductioni

Down-regulated posttranscroptionally by auxin.1 Publication

Gene expression databases

GenevisibleiQ9LFQ9. AT.

Interactioni

Subunit structurei

Monomer. No interactions with DPA or E2FA.1 Publication

Protein-protein interaction databases

BioGridi16625. 9 interactors.
STRINGi3702.AT5G14960.1.

Structurei

3D structure databases

ProteinModelPortaliQ9LFQ9.
SMRiQ9LFQ9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The two DNA binding domains are required for binding to the E2 site.

Sequence similaritiesi

Belongs to the E2F/DP family.Curated

Phylogenomic databases

eggNOGiKOG2578. Eukaryota.
ENOG4111IGY. LUCA.
HOGENOMiHOG000083291.
InParanoidiQ9LFQ9.
OMAiVIKQEQC.
OrthoDBiEOG09360FBF.
PhylomeDBiQ9LFQ9.

Family and domain databases

Gene3Di1.10.10.10. 2 hits.
InterProiIPR015633. E2F.
IPR003316. E2F_WHTH_DNA-bd_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR12081. PTHR12081. 1 hit.
PfamiPF02319. E2F_TDP. 2 hits.
[Graphical view]
SMARTiSM01372. E2F_TDP. 2 hits.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 2 hits.

Sequencei

Sequence statusi: Complete.

Q9LFQ9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDSLALAPQV YSRKDKSLGV LVANFLTLYN RPDVDLFGLD DAAAKLGVER
60 70 80 90 100
RRIYDVVNIL ESIGLVARSG KNQYSWKGFG AVPRALSELK EEGMKEKFAI
110 120 130 140 150
VPFVAKSEMV VYEKEGEESF MLSPDDQEFS PSPRPDNRKE RTLWLLAQNF
160 170 180 190 200
VKLFLCSDDD LVTFDSATKA LLNESQDMNM RKKVRRLYDI ANVFSSMKLI
210 220 230 240 250
EKTHVPETKK PAYRWLGSKT IFENRFIDGS ASLCDRNVPK KRAFGTELTN
260 270 280 290 300
VNAKRNKSGC SKEDSKRNGN QNTSIVIKQE QCDDVKPDVK NFASGSSTPA
310 320 330 340 350
GTSESNDMGN NIRPRGRLGV IEALSTLYQP SYCNPELLGL FAHYNETFRS

YQEEFGREK
Length:359
Mass (Da):40,553
Last modified:October 1, 2000 - v1
Checksum:i9E14C2933E07CF3C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ417835 mRNA. Translation: CAD10632.1.
AB074531 mRNA. Translation: BAB91412.1.
AL391146 Genomic DNA. Translation: CAC01815.1.
CP002688 Genomic DNA. Translation: AED92096.1.
AB493750 mRNA. Translation: BAH30588.1.
PIRiT51441.
RefSeqiNP_197000.1. NM_121500.4.
UniGeneiAt.31860.

Genome annotation databases

EnsemblPlantsiAT5G14960.1; AT5G14960.1; AT5G14960.
GeneIDi831348.
GrameneiAT5G14960.1; AT5G14960.1; AT5G14960.
KEGGiath:AT5G14960.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ417835 mRNA. Translation: CAD10632.1.
AB074531 mRNA. Translation: BAB91412.1.
AL391146 Genomic DNA. Translation: CAC01815.1.
CP002688 Genomic DNA. Translation: AED92096.1.
AB493750 mRNA. Translation: BAH30588.1.
PIRiT51441.
RefSeqiNP_197000.1. NM_121500.4.
UniGeneiAt.31860.

3D structure databases

ProteinModelPortaliQ9LFQ9.
SMRiQ9LFQ9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi16625. 9 interactors.
STRINGi3702.AT5G14960.1.

Proteomic databases

PaxDbiQ9LFQ9.
PRIDEiQ9LFQ9.

Protocols and materials databases

DNASUi831348.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G14960.1; AT5G14960.1; AT5G14960.
GeneIDi831348.
GrameneiAT5G14960.1; AT5G14960.1; AT5G14960.
KEGGiath:AT5G14960.

Organism-specific databases

TAIRiAT5G14960.

Phylogenomic databases

eggNOGiKOG2578. Eukaryota.
ENOG4111IGY. LUCA.
HOGENOMiHOG000083291.
InParanoidiQ9LFQ9.
OMAiVIKQEQC.
OrthoDBiEOG09360FBF.
PhylomeDBiQ9LFQ9.

Miscellaneous databases

PROiQ9LFQ9.

Gene expression databases

GenevisibleiQ9LFQ9. AT.

Family and domain databases

Gene3Di1.10.10.10. 2 hits.
InterProiIPR015633. E2F.
IPR003316. E2F_WHTH_DNA-bd_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR12081. PTHR12081. 1 hit.
PfamiPF02319. E2F_TDP. 2 hits.
[Graphical view]
SMARTiSM01372. E2F_TDP. 2 hits.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiE2FD_ARATH
AccessioniPrimary (citable) accession number: Q9LFQ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 5, 2011
Last sequence update: October 1, 2000
Last modified: November 30, 2016
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.