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Q9LFJ8 (U78D2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
UDP-glycosyltransferase 78D2

EC=2.4.1.-
Alternative name(s):
Anthocyanin 3-O-glucosyltransferase
Flavonol 3-O-glucosyltransferase.
EC=2.4.1.91
UDP glucose:flavonoid 3-O-glucosyltransferase
Gene names
Name:UGT78D2
Synonyms:AGT
Ordered Locus Names:At5g17050
ORF Names:F2K13.200
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length460 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the glycosylation of flavonoids from UDP-glucose. Catalyzes the glycosylation of anthocyanins from UDP-glucose. Possesses high quercetin 3-O-glucosyltransferase activity in vitro. Also active in vitro on benzoates and benzoate derivatives. Ref.5 Ref.6 Ref.7 Ref.8

Catalytic activity

UDP-glucose + a flavonol = UDP + a flavonol 3-O-D-glucoside.

Pathway

Secondary metabolite biosynthesis; flavonoid biosynthesis.

Induction

By PAP1. Ref.7

Sequence similarities

Belongs to the UDP-glycosyltransferase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 460460UDP-glycosyltransferase 78D2
PRO_0000285275

Regions

Region339 – 3413UDP-glucose binding By similarity
Region356 – 3649UDP-glucose binding By similarity
Region378 – 3814UDP-glucose binding By similarity

Sites

Binding site2861UDP-glucose By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9LFJ8 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: E9DEAD19E4E05188

FASTA46050,486
        10         20         30         40         50         60 
MTKPSDPTRD SHVAVLAFPF GTHAAPLLTV TRRLASASPS TVFSFFNTAQ SNSSLFSSGD 

        70         80         90        100        110        120 
EADRPANIRV YDIADGVPEG YVFSGRPQEA IELFLQAAPE NFRREIAKAE TEVGTEVKCL 

       130        140        150        160        170        180 
MTDAFFWFAA DMATEINASW IAFWTAGANS LSAHLYTDLI RETIGVKEVG ERMEETIGVI 

       190        200        210        220        230        240 
SGMEKIRVKD TPEGVVFGNL DSVFSKMLHQ MGLALPRATA VFINSFEDLD PTLTNNLRSR 

       250        260        270        280        290        300 
FKRYLNIGPL GLLSSTLQQL VQDPHGCLAW MEKRSSGSVA YISFGTVMTP PPGELAAIAE 

       310        320        330        340        350        360 
GLESSKVPFV WSLKEKSLVQ LPKGFLDRTR EQGIVVPWAP QVELLKHEAT GVFVTHCGWN 

       370        380        390        400        410        420 
SVLESVSGGV PMICRPFFGD QRLNGRAVEV VWEIGMTIIN GVFTKDGFEK CLDKVLVQDD 

       430        440        450        460 
GKKMKCNAKK LKELAYEAVS SKGRSSENFR GLLDAVVNII 

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K. expand/collapse author list , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"Phylogenetic analysis of the UDP-glycosyltransferase multigene family of Arabidopsis thaliana."
Li Y., Baldauf S., Lim E.K., Bowles D.J.
J. Biol. Chem. 276:4338-4343(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY.
[5]"The activity of Arabidopsis glycosyltransferases toward salicylic acid, 4-hydroxybenzoic acid, and other benzoates."
Lim E.K., Doucet C.J., Li Y., Elias L., Worrall D., Spencer S.P., Ross J., Bowles D.J.
J. Biol. Chem. 277:586-592(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"Arabidopsis glycosyltransferases as biocatalysts in fermentation for regioselective synthesis of diverse quercetin glucosides."
Lim E.K., Ashford D.A., Hou B., Jackson R.G., Bowles D.J.
Biotechnol. Bioeng. 87:623-631(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[7]"Functional genomics by integrated analysis of metabolome and transcriptome of Arabidopsis plants over-expressing an MYB transcription factor."
Tohge T., Nishiyama Y., Hirai M.Y., Yano M., Nakajima J., Awazuhara M., Inoue E., Takahashi H., Goodenowe D.B., Kitayama M., Noji M., Yamazaki M., Saito K.
Plant J. 42:218-235(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INDUCTION BY PAP1.
[8]"The formation of anthocyanic vacuolar inclusions in Arabidopsis thaliana and implications for the sequestration of anthocyanin pigments."
Pourcel L., Irani N.G., Lu Y., Riedl K., Schwartz S., Grotewold E.
Mol. Plant 3:78-90(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[9]"The flavonoid biosynthetic pathway in Arabidopsis: Structural and genetic diversity."
Saito K., Yonekura-Sakakibara K., Nakabayashi R., Higashi Y., Yamazaki M., Tohge T., Fernie A.R.
Plant Physiol. Biochem. 0:0-0(2013)
Cited for: REVIEW, NOMENCLATURE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL391141 Genomic DNA. Translation: CAC01718.1.
CP002688 Genomic DNA. Translation: AED92377.1.
AY072325 mRNA. Translation: AAL61932.1.
AY128739 mRNA. Translation: AAM91139.1.
PIRT51560.
RefSeqNP_197207.1. NM_121711.4.
UniGeneAt.27563.

3D structure databases

ProteinModelPortalQ9LFJ8.
SMRQ9LFJ8. Positions 10-456.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING3702.AT5G17050.1-P.

Protein family/group databases

CAZyGT1. Glycosyltransferase Family 1.

Proteomic databases

PaxDbQ9LFJ8.
PRIDEQ9LFJ8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G17050.1; AT5G17050.1; AT5G17050.
GeneID831568.
KEGGath:AT5G17050.

Organism-specific databases

TAIRAT5G17050.

Phylogenomic databases

eggNOGCOG1819.
HOGENOMHOG000237564.
InParanoidQ9LFJ8.
KOK10757.
OMAQRMNARS.
PhylomeDBQ9LFJ8.

Enzyme and pathway databases

BioCycMetaCyc:AT5G17050-MONOMER.
UniPathwayUPA00154.

Gene expression databases

GenevestigatorQ9LFJ8.

Family and domain databases

InterProIPR002213. UDP_glucos_trans.
[Graphical view]
PANTHERPTHR11926. PTHR11926. 1 hit.
PfamPF00201. UDPGT. 1 hit.
[Graphical view]
PROSITEPS00375. UDPGT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameU78D2_ARATH
AccessionPrimary (citable) accession number: Q9LFJ8
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: October 1, 2000
Last modified: July 9, 2014
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names