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Q9LFD2

- LAC8_ARATH

UniProt

Q9LFD2 - LAC8_ARATH

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Protein

Laccase-8

Gene
LAC8, At5g01040, F7J8.20
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Lignin degradation and detoxification of lignin-derived products By similarity. Involved in the flowering time inhibition.1 Publication

Catalytic activityi

4 benzenediol + O2 = 4 benzosemiquinone + 2 H2O.

Cofactori

Binds 4 copper ions per monomer By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi83 – 831Copper 1; type 2 By similarity
Metal bindingi85 – 851Copper 2; type 3 By similarity
Metal bindingi128 – 1281Copper 2; type 3 By similarity
Metal bindingi130 – 1301Copper 3; type 3 By similarity
Metal bindingi467 – 4671Copper 4; type 1 By similarity
Metal bindingi470 – 4701Copper 1; type 2 By similarity
Metal bindingi472 – 4721Copper 3; type 3 By similarity
Metal bindingi529 – 5291Copper 3; type 3 By similarity
Metal bindingi530 – 5301Copper 4; type 1 By similarity
Metal bindingi531 – 5311Copper 2; type 3 By similarity
Metal bindingi535 – 5351Copper 4; type 1 By similarity

GO - Molecular functioni

  1. copper ion binding Source: InterPro
  2. hydroquinone:oxygen oxidoreductase activity Source: UniProtKB-EC

GO - Biological processi

  1. lignin catabolic process Source: UniProtKB-KW
  2. response to copper ion Source: TAIR
  3. vegetative to reproductive phase transition of meristem Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Lignin degradation

Keywords - Ligandi

Copper, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT5G01040-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Laccase-8 (EC:1.10.3.2)
Alternative name(s):
Benzenediol:oxygen oxidoreductase 8
Diphenol oxidase 8
Urishiol oxidase 8
Gene namesi
Name:LAC8
Ordered Locus Names:At5g01040
ORF Names:F7J8.20
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 5

Organism-specific databases

TAIRiAT5G01040.

Subcellular locationi

Secretedextracellular spaceapoplast Reviewed prediction

GO - Cellular componenti

  1. apoplast Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Apoplast, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525 Reviewed predictionAdd
BLAST
Chaini26 – 584559Laccase-8PRO_0000283636Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi52 – 521N-linked (GlcNAc...) Reviewed prediction
Glycosylationi75 – 751N-linked (GlcNAc...) Reviewed prediction
Glycosylationi79 – 791N-linked (GlcNAc...) Reviewed prediction
Glycosylationi111 – 1111N-linked (GlcNAc...) Reviewed prediction
Glycosylationi236 – 2361N-linked (GlcNAc...) Reviewed prediction
Glycosylationi332 – 3321N-linked (GlcNAc...) Reviewed prediction
Glycosylationi384 – 3841N-linked (GlcNAc...) Reviewed prediction
Glycosylationi402 – 4021N-linked (GlcNAc...) Reviewed prediction
Glycosylationi449 – 4491N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Glycoprotein

Expressioni

Tissue specificityi

Predominantly expressed in tissues other than the inflorescence stem, especially in roots.2 Publications

Developmental stagei

Expressed along a developmental gradient in the inflorescence stem, with higher levels in olders organs and low levels in young tissues.1 Publication

Gene expression databases

GenevestigatoriQ9LFD2.

Structurei

3D structure databases

ProteinModelPortaliQ9LFD2.
SMRiQ9LFD2. Positions 45-575.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini33 – 149117Plastocyanin-like 1Add
BLAST
Domaini159 – 306148Plastocyanin-like 2Add
BLAST
Domaini410 – 550141Plastocyanin-like 3Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiCOG2132.
HOGENOMiHOG000241916.
InParanoidiQ9LFD2.
OMAiAYFYNIS.
PhylomeDBiQ9LFD2.

Family and domain databases

Gene3Di2.60.40.420. 3 hits.
InterProiIPR001117. Cu-oxidase.
IPR011706. Cu-oxidase_2.
IPR011707. Cu-oxidase_3.
IPR008972. Cupredoxin.
IPR017761. Laccase.
[Graphical view]
PfamiPF00394. Cu-oxidase. 1 hit.
PF07731. Cu-oxidase_2. 1 hit.
PF07732. Cu-oxidase_3. 1 hit.
[Graphical view]
SUPFAMiSSF49503. SSF49503. 4 hits.
TIGRFAMsiTIGR03389. laccase. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9LFD2-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MPRLHHYLSN QAFLVLLLFS SIASAAVVEH VLHIQDVVVK PLCKEQIIPA    50
ANGSLPGPTI NVREGDTLVV NVINNSTYNV TIHWHGVFQL KSVWMDGANM 100
ITQCPIQPGY NFTYQFDITG QEGTLLWHAH VVNLRATLHG ALVIRPRSGR 150
PYPFPKPYKE VPIVFQQWWD TDVRLLQLRP APVSDAYLIN GLAGDSYPCS 200
ENRMFNLKVV QGKTYLLRIV NAALNTHLFF KIANHNVTVV AVDAVYSTPY 250
LTDVMILTPG QTVDALLTAD QAIGKYYMAT LPYISAIGIP TPDIKPTRGL 300
IVYQGATSSS SPAEPLMPVP NDMSTAHRFT SNITSLVGGP HWTPVPRHVD 350
EKMFITMGLG LDPCPAGTKC IGPLGQRYAG SLNNRTFMIP ERISMQEAYF 400
YNISGIYTDD FPNQPPLKFD YTKFEQRTNN DMKMMFPERK TSVKKIRFNS 450
TVEIVLQNTA IISPESHPMH LHGFNFYVLG YGFGNYDPIR DARKLNLFNP 500
QMHNTVGVPP GGWVVLRFIA NNPGVWLFHC HMDAHLPYGI MSAFIVQNGP 550
TPETSLPSPP SNLPQCTRDP TIYDSRTTNI DLSY 584
Length:584
Mass (Da):65,282
Last modified:October 1, 2000 - v1
Checksum:iE83C308696325898
GO
Isoform 2 (identifier: Q9LFD2-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     203-234: RMFNLKVVQGKTYLLRIVNAALNTHLFFKIAN → SNTHSLLESKVTFYCKLFTKNRINFYFYFWLW
     235-584: Missing.

Note: No experimental confirmation available.

Show »
Length:234
Mass (Da):26,727
Checksum:i75498B1972496DA1
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei203 – 23432RMFNL…FKIAN → SNTHSLLESKVTFYCKLFTK NRINFYFYFWLW in isoform 2. VSP_024348Add
BLAST
Alternative sequencei235 – 584350Missing in isoform 2. VSP_024349Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL137189 Genomic DNA. Translation: CAB69832.1.
CP002688 Genomic DNA. Translation: AED90291.1.
AK117360 mRNA. Translation: BAC42030.1.
AK176457 mRNA. Translation: BAD44220.1.
PIRiT45944.
RefSeqiNP_195724.1. NM_120181.4. [Q9LFD2-1]
UniGeneiAt.27863.
At.27905.

Genome annotation databases

EnsemblPlantsiAT5G01040.1; AT5G01040.1; AT5G01040. [Q9LFD2-1]
GeneIDi831877.
KEGGiath:AT5G01040.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL137189 Genomic DNA. Translation: CAB69832.1 .
CP002688 Genomic DNA. Translation: AED90291.1 .
AK117360 mRNA. Translation: BAC42030.1 .
AK176457 mRNA. Translation: BAD44220.1 .
PIRi T45944.
RefSeqi NP_195724.1. NM_120181.4. [Q9LFD2-1 ]
UniGenei At.27863.
At.27905.

3D structure databases

ProteinModelPortali Q9LFD2.
SMRi Q9LFD2. Positions 45-575.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT5G01040.1 ; AT5G01040.1 ; AT5G01040 . [Q9LFD2-1 ]
GeneIDi 831877.
KEGGi ath:AT5G01040.

Organism-specific databases

TAIRi AT5G01040.

Phylogenomic databases

eggNOGi COG2132.
HOGENOMi HOG000241916.
InParanoidi Q9LFD2.
OMAi AYFYNIS.
PhylomeDBi Q9LFD2.

Enzyme and pathway databases

BioCyci ARA:AT5G01040-MONOMER.

Gene expression databases

Genevestigatori Q9LFD2.

Family and domain databases

Gene3Di 2.60.40.420. 3 hits.
InterProi IPR001117. Cu-oxidase.
IPR011706. Cu-oxidase_2.
IPR011707. Cu-oxidase_3.
IPR008972. Cupredoxin.
IPR017761. Laccase.
[Graphical view ]
Pfami PF00394. Cu-oxidase. 1 hit.
PF07731. Cu-oxidase_2. 1 hit.
PF07732. Cu-oxidase_3. 1 hit.
[Graphical view ]
SUPFAMi SSF49503. SSF49503. 4 hits.
TIGRFAMsi TIGR03389. laccase. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
    Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.
    , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
    Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: cv. Columbia.
  4. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 204-499 (ISOFORM 1).
    Strain: cv. Columbia.
  5. "Gene structure and molecular analysis of the laccase-like multicopper oxidase (LMCO) gene family in Arabidopsis thaliana."
    McCaig B.C., Meagher R.B., Dean J.F.D.
    Planta 221:619-636(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
  6. "Mutant identification and characterization of the laccase gene family in Arabidopsis."
    Cai X., Davis E.J., Ballif J., Liang M., Bushman E., Haroldsen V., Torabinejad J., Wu Y.
    J. Exp. Bot. 57:2563-2569(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiLAC8_ARATH
AccessioniPrimary (citable) accession number: Q9LFD2
Secondary accession number(s): Q67YL1, Q8GYV9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: October 1, 2000
Last modified: May 14, 2014
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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