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Protein

Protein NRT1/ PTR FAMILY 8.2

Gene

NPF8.2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Peptide transporter. Mediates the transport of di- and tripeptides. High affinity transporter. Involved in the uptake of peptides during pollen germination and tube growth.1 Publication

GO - Molecular functioni

  • dipeptide transporter activity Source: TAIR

GO - Biological processi

  • dipeptide transport Source: TAIR
  • pollen tube growth Source: TAIR
  • protein transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Peptide transport, Protein transport, Transport

Enzyme and pathway databases

BRENDAi3.6.3.43. 399.

Protein family/group databases

TCDBi2.A.17.3.8. the proton-dependent oligopeptide transporter (pot/ptr) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein NRT1/ PTR FAMILY 8.2
Short name:
AtNPF8.2
Alternative name(s):
Peptide transporter PTR5
Gene namesi
Name:NPF8.2
Synonyms:PTR5
Ordered Locus Names:At5g01180
ORF Names:F7J8.160
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G01180.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei100 – 120HelicalSequence analysisAdd BLAST21
Transmembranei136 – 156HelicalSequence analysisAdd BLAST21
Transmembranei182 – 202HelicalSequence analysisAdd BLAST21
Transmembranei210 – 230HelicalSequence analysisAdd BLAST21
Transmembranei335 – 355HelicalSequence analysisAdd BLAST21
Transmembranei370 – 390HelicalSequence analysisAdd BLAST21
Transmembranei414 – 434HelicalSequence analysisAdd BLAST21
Transmembranei454 – 474HelicalSequence analysisAdd BLAST21
Transmembranei493 – 513HelicalSequence analysisAdd BLAST21
Transmembranei537 – 557HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

No visible phenotype.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003783231 – 570Protein NRT1/ PTR FAMILY 8.2Add BLAST570

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei99PhosphothreonineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9LFB8.

Expressioni

Tissue specificityi

Expressed in developing and germinating pollen grains and ovules.2 Publications

Developmental stagei

Expressed during early seed development.1 Publication

Gene expression databases

GenevisibleiQ9LFB8. AT.

Interactioni

Protein-protein interaction databases

BioGridi17001. 25 interactors.
STRINGi3702.AT5G01180.1.

Structurei

3D structure databases

ProteinModelPortaliQ9LFB8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1237. Eukaryota.
COG3104. LUCA.
HOGENOMiHOG000237400.
InParanoidiQ9LFB8.
OMAiQTINSST.
OrthoDBiEOG093606MH.
PhylomeDBiQ9LFB8.

Family and domain databases

CDDicd06174. MFS. 1 hit.
InterProiIPR020846. MFS_dom.
IPR000109. POT_fam.
IPR018456. PTR2_symporter_CS.
[Graphical view]
PANTHERiPTHR11654. PTHR11654. 1 hit.
PfamiPF00854. PTR2. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 2 hits.
PROSITEiPS01022. PTR2_1. 1 hit.
PS01023. PTR2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9LFB8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEDDKDIYTK DGTLDIHKKP ANKNKTGTWK ACRFILGTEC CERLAYYGMS
60 70 80 90 100
TNLINYLEKQ MNMENVSASK SVSNWSGTCY ATPLIGAFIA DAYLGRYWTI
110 120 130 140 150
ASFVVIYIAG MTLLTISASV PGLTPTCSGE TCHATAGQTA ITFIALYLIA
160 170 180 190 200
LGTGGIKPCV SSFGADQFDD TDEKEKESKS SFFNWFYFVI NVGAMIASSV
210 220 230 240 250
LVWIQMNVGW GWGLGVPTVA MAIAVVFFFA GSNFYRLQKP GGSPLTRMLQ
260 270 280 290 300
VIVASCRKSK VKIPEDESLL YENQDAESSI IGSRKLEHTK ILTFFDKAAV
310 320 330 340 350
ETESDNKGAA KSSSWKLCTV TQVEELKALI RLLPIWATGI VFASVYSQMG
360 370 380 390 400
TVFVLQGNTL DQHMGPNFKI PSASLSLFDT LSVLFWAPVY DKLIVPFARK
410 420 430 440 450
YTGHERGFTQ LQRIGIGLVI SIFSMVSAGI LEVARLNYVQ THNLYNEETI
460 470 480 490 500
PMTIFWQVPQ YFLVGCAEVF TFIGQLEFFY DQAPDAMRSL CSALSLTAIA
510 520 530 540 550
FGNYLSTFLV TLVTKVTRSG GRPGWIAKNL NNGHLDYFFW LLAGLSFLNF
560 570
LVYLWIAKWY TYKKTTGHAL
Length:570
Mass (Da):63,308
Last modified:October 1, 2000 - v1
Checksum:i4603B9B64BB20CF5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti302T → A in AAO64143 (PubMed:14593172).Curated1
Sequence conflicti302T → A in BAF00365 (Ref. 4) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL137189 Genomic DNA. Translation: CAB69846.1.
CP002688 Genomic DNA. Translation: AED90307.1.
BT005728 mRNA. Translation: AAO64143.1.
AK228435 mRNA. Translation: BAF00365.1.
PIRiT45958.
RefSeqiNP_195738.1. NM_120196.4.
UniGeneiAt.33937.

Genome annotation databases

EnsemblPlantsiAT5G01180.1; AT5G01180.1; AT5G01180.
GeneIDi831725.
GrameneiAT5G01180.1; AT5G01180.1; AT5G01180.
KEGGiath:AT5G01180.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL137189 Genomic DNA. Translation: CAB69846.1.
CP002688 Genomic DNA. Translation: AED90307.1.
BT005728 mRNA. Translation: AAO64143.1.
AK228435 mRNA. Translation: BAF00365.1.
PIRiT45958.
RefSeqiNP_195738.1. NM_120196.4.
UniGeneiAt.33937.

3D structure databases

ProteinModelPortaliQ9LFB8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi17001. 25 interactors.
STRINGi3702.AT5G01180.1.

Protein family/group databases

TCDBi2.A.17.3.8. the proton-dependent oligopeptide transporter (pot/ptr) family.

Proteomic databases

PaxDbiQ9LFB8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G01180.1; AT5G01180.1; AT5G01180.
GeneIDi831725.
GrameneiAT5G01180.1; AT5G01180.1; AT5G01180.
KEGGiath:AT5G01180.

Organism-specific databases

TAIRiAT5G01180.

Phylogenomic databases

eggNOGiKOG1237. Eukaryota.
COG3104. LUCA.
HOGENOMiHOG000237400.
InParanoidiQ9LFB8.
OMAiQTINSST.
OrthoDBiEOG093606MH.
PhylomeDBiQ9LFB8.

Enzyme and pathway databases

BRENDAi3.6.3.43. 399.

Miscellaneous databases

PROiQ9LFB8.

Gene expression databases

GenevisibleiQ9LFB8. AT.

Family and domain databases

CDDicd06174. MFS. 1 hit.
InterProiIPR020846. MFS_dom.
IPR000109. POT_fam.
IPR018456. PTR2_symporter_CS.
[Graphical view]
PANTHERiPTHR11654. PTHR11654. 1 hit.
PfamiPF00854. PTR2. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 2 hits.
PROSITEiPS01022. PTR2_1. 1 hit.
PS01023. PTR2_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPTR5_ARATH
AccessioniPrimary (citable) accession number: Q9LFB8
Secondary accession number(s): Q0WR84, Q84TJ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 16, 2009
Last sequence update: October 1, 2000
Last modified: November 30, 2016
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.