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Q9LFB2 (LAX1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Auxin transporter-like protein 1
Alternative name(s):
AUX1-like protein 1
Gene names
Name:LAX1
Ordered Locus Names:At5g01240
ORF Names:F7J8_220
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length488 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Carrier protein involved in proton-driven auxin influx. Mediates the formation of auxin gradient from developing leaves (site of auxin biosynthesis) to tips by contributing to the loading of auxin in vascular tissues and facilitating acropetal (base to tip) auxin transport within inner tissues of the root apex, and basipetal (tip to base) auxin transport within outer tissues of the root apex By similarity.

Subcellular location

Cell membrane; Multi-pass membrane protein By similarity.

Sequence similarities

Belongs to the amino acid/polyamine transporter 2 family. Amino acid/auxin permease (AAAP) (TC 2.A.18.1) subfamily. [View classification]

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q9LFB2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 488488Auxin transporter-like protein 1
PRO_0000093842

Regions

Topological domain1 – 6464Cytoplasmic Potential
Transmembrane65 – 8218Helical; Potential
Topological domain83 – 842Extracellular Potential
Transmembrane85 – 10521Helical; Potential
Topological domain106 – 14136Cytoplasmic Potential
Transmembrane142 – 16221Helical; Potential
Topological domain163 – 17816Extracellular Potential
Transmembrane179 – 19921Helical; Potential
Topological domain200 – 2023Cytoplasmic Potential
Transmembrane203 – 22321Helical; Potential
Topological domain224 – 23815Extracellular Potential
Transmembrane239 – 25921Helical; Potential
Topological domain260 – 27314Cytoplasmic Potential
Transmembrane274 – 29421Helical; Potential
Topological domain295 – 32026Extracellular Potential
Transmembrane321 – 34121Helical; Potential
Topological domain342 – 36221Cytoplasmic Potential
Transmembrane363 – 38321Helical; Potential
Topological domain3841Extracellular Potential
Transmembrane385 – 40521Helical; Potential
Topological domain406 – 42722Cytoplasmic Potential
Transmembrane428 – 44821Helical; Potential
Topological domain449 – 48840Extracellular Potential

Amino acid modifications

Glycosylation3031N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict16 – 172ED → DE in CAB55758. Ref.1
Sequence conflict2931V → L in CAB55758. Ref.1
Sequence conflict365 – 3662VV → C in CAB55758. Ref.1
Sequence conflict372 – 3732LA → WP in CAB55758. Ref.1
Sequence conflict379 – 3813FGP → SA in CAB55758. Ref.1
Sequence conflict413 – 4164RRNA → AER in CAB55758. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 06B0BF524EDE2BD8

FASTA48854,601
        10         20         30         40         50         60 
MSGEKQAEES IVVSGEDEVA GRKVEDSAAE EDIDGNGGNG FSMKSFLWHG GSAWDAWFSC 

        70         80         90        100        110        120 
ASNQVAQVLL TLPYSFSQLG MLSGILLQIF YGLMGSWTAY LISVLYVEYR ARMEKQEAKS 

       130        140        150        160        170        180 
FKNHVIQWFE VLDGLLGPYW KAAGLAFNCT FLLFGSVIQL IACASNIYYI NDRLDKRTWT 

       190        200        210        220        230        240 
YIFGACCATT VFIPSFHNYR IWSFLGLGMT TYTAWYLTIA SFLHGQAEGV THSGPTKLVL 

       250        260        270        280        290        300 
YFTGATNILY TFGGHAVTVE IMHAMWKPRK FKSIYLMATL YVFTLTLPSA SAVYWAFGDQ 

       310        320        330        340        350        360 
LLNHSNAFSL LPKTRFRDTA VILMLIHQFI TFGFACTPLY FVWEKAIGMH HTKSLCLRAL 

       370        380        390        400        410        420 
VRLPVVVPIW FLAIIFPFFG PINSAVGALL VTFTVYIIPA LAHMLTYRTA SARRNAAEKP 

       430        440        450        460        470        480 
PFFIPSWAGV YVINAFIVVW VLVLGFGFGG WASMTNFIRQ IDTFGLFAKC YQCKPPPAPI 


AAGAHHRR 

« Hide

References

« Hide 'large scale' references
[1]"Quick on the uptake: characterization of a family of plant auxin influx carriers."
Parry P.G., Marchant A., May S.T., Swarup R., Swarup K., James N., Graham N., Allen T., Martucci T., Yemm A., Napier R., Manning K., King G., Bennett M.J.
J. Plant Growth Regul. 20:217-225(2001)
Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, NOMENCLATURE.
[2]"Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K. expand/collapse author list , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ249442 mRNA. Translation: CAB55758.1.
AL137189 Genomic DNA. Translation: CAB69852.1.
CP002688 Genomic DNA. Translation: AED90315.1.
PIRT45964.
RefSeqNP_195744.1. NM_120202.4. [Q9LFB2-1]
UniGeneAt.67248.
At.9820.

3D structure databases

ProteinModelPortalQ9LFB2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

MINTMINT-8066356.
STRING3702.AT5G01240.1-P.

Proteomic databases

PaxDbQ9LFB2.
PRIDEQ9LFB2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G01240.1; AT5G01240.1; AT5G01240. [Q9LFB2-1]
GeneID831866.
KEGGath:AT5G01240.

Organism-specific databases

GeneFarm5109.
TAIRAT5G01240.

Phylogenomic databases

eggNOGKOG1303.
HOGENOMHOG000238482.
InParanoidQ9LFB2.
KOK13946.
OMAYVINAFI.
PhylomeDBQ9LFB2.

Gene expression databases

GenevestigatorQ9LFB2.

Family and domain databases

InterProIPR013057. AA_transpt_TM.
[Graphical view]
PfamPF01490. Aa_trans. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameLAX1_ARATH
AccessionPrimary (citable) accession number: Q9LFB2
Secondary accession number(s): Q9SMW2
Entry history
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: October 1, 2000
Last modified: May 14, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names