Reviewed, UniProtKB/Swiss-Prot Q9LFA3 (MDAR3_ARATH)

Names and origin

Protein names

Recommended name:
    Probable monodehydroascorbate reductase, cytoplasmic isoform 3
      Short name=MDAR 3
    EC=1.6.5.4

Gene names

Ordered Locus Names:	At3g52880
ORF Names:	F8J2_50

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	434 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the conversion of monodehydroascorbate to ascorbate, oxidizing NADH in the process By similarity.
Catalytic activity	NADH + 2 monodehydroascorbate = NAD+ + 2 ascorbate.
Cofactor	FAD By similarity.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the FAD-dependent oxidoreductase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Domain	Redox-active center
Ligand	FAD Flavoprotein NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Traceable author statement. Source: TAIR oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW response to cadmium ion Inferred from expression pattern. Source: TAIR
Cellular component	apoplast Inferred from direct assay. Source: TAIR chloroplast Inferred from direct assay. Source: TAIR peroxisomal matrix Inferred from direct assay. Source: TAIR plasma membrane Inferred from direct assay. Source: TAIR
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro monodehydroascorbate reductase (NADH) activity Inferred from direct assay. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 434	434	Probable monodehydroascorbate reductase, cytoplasmic isoform 3		PRO_0000209138
Experimental info
	Sequence conflict	206	1	D → N in AAM64531. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q9LFA3-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: DE7FFF0F349784A0	FASTA	434	46,487
	10 20 30 40 50 60 MAEKSFKYII LGGGVSAGYA AKEFANQGVQ PGELAVISKE AVAPYERPAL SKGYLFPEGA 70 80 90 100 110 120 ARLPGFHCCV GSGGEKLLPE SYKQKGIELI LSTEIVKADL SAKSLVSATG DVFKYQTLII 130 140 150 160 170 180 ATGSTVLRLT DFGVKGADSK NILYLREIDD ADKLVEAIKA KKGGKAVVVG GGYIGLELSA 190 200 210 220 230 240 VLRINNLDVT MVFPEPWCMP RLFTADIAAF YETYYTNKGV KIIKGTVASG FTAQPNGEVK 250 260 270 280 290 300 EVQLKDGRTL EADIVIVGVG AKPLTSLFKG QVEEDKGGIK TDAFFKTSVP DVYAVGDVAT 310 320 330 340 350 360 FPLKMYGDVR RVEHVDHSRK SAEQAVKAIK AAEGGAAVEE YDYLPFFYSR SFDLSWQFYG 370 380 390 400 410 420 DNVGDSVLFG DSNPSNPKPR FGAYWVQGGK VVGAFMEGGS GDENKALAKV AKARPSAESL 430 DELVKQGISF AAKI

References

[1]	"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana." Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. Nature 408:820-822(2000) [PubMed: 11130713] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[3]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL132969 Genomic DNA. Translation: CAB86892.1. AF360197 mRNA. Translation: AAK25907.1. AY057576 mRNA. Translation: AAL09815.1. AY045666 mRNA. Translation: AAK74024.1. AY070718 mRNA. Translation: AAL50062.1. AY060525 mRNA. Translation: AAL31138.1. AY091403 mRNA. Translation: AAM14342.1. AY125492 mRNA. Translation: AAM83213.1. AY086968 mRNA. Translation: AAM64531.1.
IPI	IPI00543137.
PIR	T47545.
RefSeq	NP_190856.1.
UniGene	At.24483 At.67871
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	Q9LFA3.
Genome annotation databases
GeneID	824454.
GenomeReviews	Gene locus AT3G52880 in contig BA000014_GR.
KEGG	ath:AT3G52880.
NMPDR	fig|3702.1.peg.16560.
Organism-specific databases
TAIR	At3g52880.
Phylogenomic databases
OMA	Q9LFA3. SAGWFAP.
Enzyme and pathway databases
BRENDA	1.6.5.4. 302.
Gene expression databases
ArrayExpress	Q9LFA3.
GermOnline	AT3G52880. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR001327. Pyr_OxRdtase_NAD_bd. [Graphical view]
Pfam	PF00070. Pyr_redox. 1 hit. PF07992. Pyr_redox_2. 1 hit. [Graphical view]
PRINTS	PR00368. FADPNR.
ProDom	PD000139. FAD_pyr_redox. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

MDAR3_ARATH

Accession

Primary (citable) accession number: Q9LFA3
Secondary accession number(s): Q8LBV9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2004
Last sequence update:	October 1, 2000
Last modified:	June 16, 2009
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)