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Protein

Monodehydroascorbate reductase 1, peroxisomal

Gene

MDAR1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of monodehydroascorbate to ascorbate, oxidizing NADH in the process.1 Publication

Catalytic activityi

NADH + 2 monodehydroascorbate = NAD+ + 2 ascorbate.Curated

Cofactori

FADBy similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei40FADBy similarity1
Binding sitei47FADBy similarity1
Binding sitei52FADBy similarity1
Binding sitei95FAD; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei195NADBy similarity1
Binding sitei201NADBy similarity1
Binding sitei201NADPBy similarity1
Binding sitei260NAD; via amide nitrogenBy similarity1
Binding sitei260NADP; via amide nitrogenBy similarity1
Binding sitei297FADBy similarity1
Binding sitei315FAD; via amide nitrogenBy similarity1
Binding sitei319AscorbateBy similarity1
Binding sitei348FAD; via carbonyl oxygenBy similarity1
Binding sitei348NAD; via carbonyl oxygenBy similarity1
Binding sitei348NADP; via carbonyl oxygenBy similarity1
Binding sitei350AscorbateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi13 – 16FADBy similarity4
Nucleotide bindingi146 – 147FADBy similarity2
Nucleotide bindingi171 – 177NADBy similarity7
Nucleotide bindingi173 – 177NADPBy similarity5
Nucleotide bindingi313 – 314NADBy similarity2
Nucleotide bindingi313 – 314NADPBy similarity2

GO - Molecular functioni

Keywordsi

Molecular functionOxidoreductase
LigandFAD, Flavoprotein, NAD, NADP

Enzyme and pathway databases

BioCyciARA:AT3G52880-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Monodehydroascorbate reductase 1, peroxisomal1 Publication (EC:1.6.5.4Curated)
Short name:
AtMDAR11 Publication
Gene namesi
Name:MDAR11 Publication
Ordered Locus Names:At3g52880Imported
ORF Names:F8J2.50Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G52880.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Peroxisome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi432 – 434Missing : Loss of peroxisomal targeting. 1 Publication3

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002091381 – 434Monodehydroascorbate reductase 1, peroxisomalAdd BLAST434

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei416PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiQ9LFA3.

PTM databases

iPTMnetiQ9LFA3.

Expressioni

Gene expression databases

ExpressionAtlasiQ9LFA3. baseline and differential.
GenevisibleiQ9LFA3. AT.

Interactioni

Protein-protein interaction databases

BioGridi9771. 1 interactor.

Structurei

3D structure databases

ProteinModelPortaliQ9LFA3.
SMRiQ9LFA3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Redox-active center

Phylogenomic databases

HOGENOMiHOG000276711.
InParanoidiQ9LFA3.
KOiK08232.
PhylomeDBiQ9LFA3.

Family and domain databases

Gene3Di3.30.390.30. 1 hit.
3.50.50.60. 3 hits.
InterProiView protein in InterPro
IPR036188. FAD/NAD-bd_sf.
IPR023753. FAD/NAD-binding_dom.
IPR016156. FAD/NAD-linked_Rdtase_dimer_sf.
PfamiView protein in Pfam
PF07992. Pyr_redox_2. 1 hit.
SUPFAMiSSF51905. SSF51905. 1 hit.
SSF55424. SSF55424. 1 hit.

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q9LFA3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAEKSFKYII LGGGVSAGYA AKEFANQGVQ PGELAVISKE AVAPYERPAL
60 70 80 90 100
SKGYLFPEGA ARLPGFHCCV GSGGEKLLPE SYKQKGIELI LSTEIVKADL
110 120 130 140 150
SAKSLVSATG DVFKYQTLII ATGSTVLRLT DFGVKGADSK NILYLREIDD
160 170 180 190 200
ADKLVEAIKA KKGGKAVVVG GGYIGLELSA VLRINNLDVT MVFPEPWCMP
210 220 230 240 250
RLFTADIAAF YETYYTNKGV KIIKGTVASG FTAQPNGEVK EVQLKDGRTL
260 270 280 290 300
EADIVIVGVG AKPLTSLFKG QVEEDKGGIK TDAFFKTSVP DVYAVGDVAT
310 320 330 340 350
FPLKMYGDVR RVEHVDHSRK SAEQAVKAIK AAEGGAAVEE YDYLPFFYSR
360 370 380 390 400
SFDLSWQFYG DNVGDSVLFG DSNPSNPKPR FGAYWVQGGK VVGAFMEGGS
410 420 430
GDENKALAKV AKARPSAESL DELVKQGISF AAKI
Length:434
Mass (Da):46,487
Last modified:October 1, 2000 - v1
Checksum:iDE7FFF0F349784A0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti206D → N in AAM64531 (Ref. 4) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL132969 Genomic DNA. Translation: CAB86892.1.
CP002686 Genomic DNA. Translation: AEE79003.1.
AF360197 mRNA. Translation: AAK25907.1.
AY057576 mRNA. Translation: AAL09815.1.
AY045666 mRNA. Translation: AAK74024.1.
AY070718 mRNA. Translation: AAL50062.1.
AY060525 mRNA. Translation: AAL31138.1.
AY091403 mRNA. Translation: AAM14342.1.
AY125492 mRNA. Translation: AAM83213.1.
AY086968 mRNA. Translation: AAM64531.1.
AK227030 mRNA. Translation: BAE99092.1.
AK317341 mRNA. Translation: BAH20014.1.
PIRiT47545.
RefSeqiNP_190856.1. NM_115148.4. [Q9LFA3-1]
UniGeneiAt.24483.
At.67871.

Genome annotation databases

EnsemblPlantsiAT3G52880.1; AT3G52880.1; AT3G52880. [Q9LFA3-1]
GeneIDi824454.
GrameneiAT3G52880.1; AT3G52880.1; AT3G52880.
KEGGiath:AT3G52880.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiMDAR1_ARATH
AccessioniPrimary (citable) accession number: Q9LFA3
Secondary accession number(s): Q0WUV6, Q8LBV9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: October 1, 2000
Last modified: November 22, 2017
This is version 119 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families