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Protein

Fructose-bisphosphate aldolase 8, cytosolic

Gene

FBA8

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Fructose-bisphosphate aldolase that plays a key role in glycolysis and gluconeogenesis.By similarity

Catalytic activityi

D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.By similarity

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.Curated
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (AXX17_At5g40630), Glucose-6-phosphate isomerase (PGI1 (At4g24620)), Glucose-6-phosphate isomerase, cytosolic (PGIC), Glucose-6-phosphate isomerase (AXX17_At4g28500), Glucose-6-phosphate isomerase 1, chloroplastic (PGI1), Glucose-6-phosphate isomerase (PGIC)
  3. ATP-dependent 6-phosphofructokinase 1 (PFK1), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase 3 (PFK3), ATP-dependent 6-phosphofructokinase 4, chloroplastic (PFK4), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha (PFP-ALPHA), ATP-dependent 6-phosphofructokinase 2 (PFK2), ATP-dependent 6-phosphofructokinase (PFK), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta 2 (PFP-BETA2), ATP-dependent 6-phosphofructokinase 5, chloroplastic (PFK5), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha (PFP-ALPHA), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase (PFK), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha 1 (PFP-ALPHA1), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta (PFP-BETA), ATP-dependent 6-phosphofructokinase 6 (PFK6), ATP-dependent 6-phosphofructokinase (PFK), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha 2 (PFP-ALPHA2), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta (PFP-BETA), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta 1 (PFP-BETA1), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase 7 (PFK7), ATP-dependent 6-phosphofructokinase (PFK)
  4. Fructose-bisphosphate aldolase 5, cytosolic (FBA5), Fructose-bisphosphate aldolase (At2g36460), Fructose-bisphosphate aldolase (AXX17_At4g44320), Fructose-bisphosphate aldolase (AXX17_At4g30610), Fructose-bisphosphate aldolase (FBA2), Fructose-bisphosphate aldolase 1, chloroplastic (FBA1), Fructose-bisphosphate aldolase (AXX17_At5g03010), Fructose-bisphosphate aldolase 7, cytosolic (FBA7), Fructose-bisphosphate aldolase 3, chloroplastic (FBA3), Fructose-bisphosphate aldolase 6, cytosolic (FAB6), Fructose-bisphosphate aldolase (AXX17_At2g00100), Fructose-bisphosphate aldolase 2, chloroplastic (FBA2), Fructose-bisphosphate aldolase (At2g21330), Fructose-bisphosphate aldolase (AXX17_At2g33200), Fructose-bisphosphate aldolase (AXX17_At3g47340), Fructose-bisphosphate aldolase (FBA1), Fructose-bisphosphate aldolase (FBA1), Fructose-bisphosphate aldolase (AXX17_At2g16800), Fructose-bisphosphate aldolase (AXX17_At5g03010), Fructose-bisphosphate aldolase 8, cytosolic (FBA8), Fructose-bisphosphate aldolase (AXX17_At4g30600), Fructose-bisphosphate aldolase (AXX17_At3g47340), Fructose-bisphosphate aldolase (AXX17_At5g03010), Fructose-bisphosphate aldolase 4, cytosolic (FBA4)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei39SubstrateBy similarity1
Active sitei183Proton acceptorBy similarity1
Active sitei225Schiff-base intermediate with dihydroxyacetone-PBy similarity1
Binding sitei298SubstrateBy similarity1
Sitei358Necessary for preference for fructose 1,6-bisphosphate over fructose 1-phosphateBy similarity1

GO - Molecular functioni

  • copper ion binding Source: TAIR
  • fructose-bisphosphate aldolase activity Source: UniProtKB-EC

GO - Biological processi

  • glycolytic process Source: UniProtKB-UniPathway
  • response to cadmium ion Source: TAIR
  • response to karrikin Source: TAIR
  • response to salt stress Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

Schiff base

Enzyme and pathway databases

BioCyciARA:AT3G52930-MONOMER.
ReactomeiR-ATH-114608. Platelet degranulation.
R-ATH-6798695. Neutrophil degranulation.
R-ATH-70171. Glycolysis.
R-ATH-70263. Gluconeogenesis.
R-ATH-70350. Fructose catabolism.
UniPathwayiUPA00109; UER00183.

Names & Taxonomyi

Protein namesi
Recommended name:
Fructose-bisphosphate aldolase 8, cytosolicCurated (EC:4.1.2.13By similarity)
Short name:
AtFBA81 Publication
Alternative name(s):
Cytosolic aldolase 1Curated
Short name:
cAld1Curated
Gene namesi
Name:FBA8
Ordered Locus Names:At3g52930Imported
ORF Names:F8J2_100Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G52930.

Subcellular locationi

GO - Cellular componenti

  • apoplast Source: TAIR
  • cell wall Source: TAIR
  • chloroplast Source: TAIR
  • cytosol Source: TAIR
  • mitochondrial envelope Source: TAIR
  • mitochondrion Source: TAIR
  • nucleolus Source: TAIR
  • plasma membrane Source: TAIR
  • plasmodesma Source: TAIR
  • vacuolar membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Sterility.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00004372412 – 358Fructose-bisphosphate aldolase 8, cytosolicAdd BLAST357

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei68S-glutathionyl cysteine; transientBy similarity1
Modified residuei173S-glutathionyl cysteine; transient; alternateBy similarity1
Modified residuei173S-nitrosocysteine; transient; alternateBy similarity1
Modified residuei350PhosphoserineCombined sources1
Modified residuei354N6,N6,N6-trimethyllysineBy similarity1

Post-translational modificationi

S-glutathionylated at Cys-68 and Cys-173.By similarity
S-nitrosylated at Cys-173.By similarity

Keywords - PTMi

Acetylation, Glutathionylation, Methylation, Phosphoprotein, S-nitrosylation

Proteomic databases

PRIDEiQ9LF98.
ProMEXiQ9LF98.

Expressioni

Tissue specificityi

Highly expressed in flowers.1 Publication

Inductioni

By glucose, fructose and sucrose (PubMed:22561114). Induced by abiotic stresses (PubMed:22561114).1 Publication

Interactioni

Subunit structurei

Homotetramer (By similarity). Interacts with TRX3 (PubMed:15352244).By similarity1 Publication

Protein-protein interaction databases

IntActiQ9LF98. 4 interactors.
MINTiMINT-8060856.
STRINGi3702.AT3G52930.1.

Structurei

3D structure databases

ProteinModelPortaliQ9LF98.
SMRiQ9LF98.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni266 – 268Substrate bindingBy similarity3

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1557. Eukaryota.
COG3588. LUCA.
HOGENOMiHOG000220876.
KOiK01623.
OMAiANCQAAQ.
OrthoDBiEOG09360ENE.
PhylomeDBiQ9LF98.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR029768. Aldolase_I_AS.
IPR013785. Aldolase_TIM.
IPR000741. FBA_I.
[Graphical view]
PfamiPF00274. Glycolytic. 1 hit.
[Graphical view]
PROSITEiPS00158. ALDOLASE_CLASS_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9LF98-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAFTSKFAD ELIANAAYIG TPGKGILAAD ESTGTIGKRL ASINVENVET
60 70 80 90 100
NRRNLRELLF TAPGALPCLS GVILFEETLY QKSSDGKLFV DILKEGGVLP
110 120 130 140 150
GIKVDKGTVE LAGTDGETTT QGLDGLGDRC KKYYEAGARF AKWRAVLKIG
160 170 180 190 200
ENEPSEHSIH ENAYGLARYA VICQENGLVP IVEPEILVDG SHDIQKCAAV
210 220 230 240 250
TERVLAACYK ALSDHHVLLE GTLLKPNMVT PGSDSPKVSP EVIAEHTVRA
260 270 280 290 300
LQRTVPAAVP AIVFLSGGQS EEEATRNLNA MNQLKTKKPW SLSFSFGRAL
310 320 330 340 350
QQSTLKTWAG KEENVKAAQE ALYVRCKANS EATLGTYKGD AKLGDGAAES

LHVKDYKY
Length:358
Mass (Da):38,540
Last modified:October 1, 2000 - v1
Checksum:i0E25995B2EE0A319
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL132969 Genomic DNA. Translation: CAB86897.1.
CP002686 Genomic DNA. Translation: AEE79012.1.
AY052709 mRNA. Translation: AAK96613.1.
AY057656 mRNA. Translation: AAL15287.1.
AY063718 mRNA. Translation: AAL36068.1.
AK317380 mRNA. Translation: BAH20051.1.
AY087346 mRNA. Translation: AAM64896.1.
PIRiT47550.
RefSeqiNP_190861.1. NM_115153.4.
UniGeneiAt.25299.
At.75318.

Genome annotation databases

EnsemblPlantsiAT3G52930.1; AT3G52930.1; AT3G52930.
GeneIDi824459.
GrameneiAT3G52930.1; AT3G52930.1; AT3G52930.
KEGGiath:AT3G52930.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL132969 Genomic DNA. Translation: CAB86897.1.
CP002686 Genomic DNA. Translation: AEE79012.1.
AY052709 mRNA. Translation: AAK96613.1.
AY057656 mRNA. Translation: AAL15287.1.
AY063718 mRNA. Translation: AAL36068.1.
AK317380 mRNA. Translation: BAH20051.1.
AY087346 mRNA. Translation: AAM64896.1.
PIRiT47550.
RefSeqiNP_190861.1. NM_115153.4.
UniGeneiAt.25299.
At.75318.

3D structure databases

ProteinModelPortaliQ9LF98.
SMRiQ9LF98.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9LF98. 4 interactors.
MINTiMINT-8060856.
STRINGi3702.AT3G52930.1.

Proteomic databases

PRIDEiQ9LF98.
ProMEXiQ9LF98.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G52930.1; AT3G52930.1; AT3G52930.
GeneIDi824459.
GrameneiAT3G52930.1; AT3G52930.1; AT3G52930.
KEGGiath:AT3G52930.

Organism-specific databases

TAIRiAT3G52930.

Phylogenomic databases

eggNOGiKOG1557. Eukaryota.
COG3588. LUCA.
HOGENOMiHOG000220876.
KOiK01623.
OMAiANCQAAQ.
OrthoDBiEOG09360ENE.
PhylomeDBiQ9LF98.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00183.
BioCyciARA:AT3G52930-MONOMER.
ReactomeiR-ATH-114608. Platelet degranulation.
R-ATH-6798695. Neutrophil degranulation.
R-ATH-70171. Glycolysis.
R-ATH-70263. Gluconeogenesis.
R-ATH-70350. Fructose catabolism.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR029768. Aldolase_I_AS.
IPR013785. Aldolase_TIM.
IPR000741. FBA_I.
[Graphical view]
PfamiPF00274. Glycolytic. 1 hit.
[Graphical view]
PROSITEiPS00158. ALDOLASE_CLASS_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiALFC8_ARATH
AccessioniPrimary (citable) accession number: Q9LF98
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2016
Last sequence update: October 1, 2000
Last modified: November 30, 2016
This is version 137 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.