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Q9LF79 (ACA8_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 130. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Calcium-transporting ATPase 8, plasma membrane-type

EC=3.6.3.8
Alternative name(s):
Ca(2+)-ATPase isoform 8
Gene names
Name:ACA8
Ordered Locus Names:At5g57110
ORF Names:MUL3.5
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length1074 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol out of the cell.

Catalytic activity

ATP + H2O + Ca2+(Side 1) = ADP + phosphate + Ca2+(Side 2).

Enzyme regulation

Activated by calmodulin By similarity.

Subcellular location

Cell membrane; Multi-pass membrane protein.

Domain

The N-terminus contains an autoinhibitory calmodulin-binding domain, which binds calmodulin in a calcium-dependent fashion. Ref.1

Sequence similarities

Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIB subfamily. [View classification]

Sequence caution

The sequence BAA97361.1 differs from that shown. Reason: Erroneous gene model prediction.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

CALMP6215714EBI-980643,EBI-397403From a different organism.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10741074Calcium-transporting ATPase 8, plasma membrane-type
PRO_0000046414

Regions

Topological domain1 – 180180Cytoplasmic Potential
Transmembrane181 – 20121Helical; Potential
Topological domain202 – 21918Extracellular Potential
Transmembrane220 – 24021Helical; Potential
Topological domain241 – 369129Cytoplasmic Potential
Transmembrane370 – 38920Helical; Potential
Topological domain390 – 42637Extracellular Potential
Transmembrane427 – 44418Helical; Potential
Topological domain445 – 840396Cytoplasmic Potential
Transmembrane841 – 85919Helical; Potential
Topological domain860 – 87011Extracellular Potential
Transmembrane871 – 89121Helical; Potential
Topological domain892 – 91120Cytoplasmic Potential
Transmembrane912 – 93423Helical; Potential
Topological domain935 – 94915Extracellular Potential
Transmembrane950 – 97122Helical; Potential
Topological domain972 – 98918Cytoplasmic Potential
Transmembrane990 – 101122Helical; Potential
Topological domain1012 – 102110Extracellular Potential
Transmembrane1022 – 104322Helical; Potential
Topological domain1044 – 107431Cytoplasmic Potential
Region43 – 5412Interaction with calmodulin

Sites

Active site48214-aspartylphosphate intermediate By similarity
Metal binding7851Magnesium By similarity
Metal binding7891Magnesium By similarity

Secondary structure

... 1074
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q9LF79 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 2E86572F44DEA8A5

FASTA1,074116,174
        10         20         30         40         50         60 
MTSLLKSSPG RRRGGDVESG KSEHADSDSD TFYIPSKNAS IERLQQWRKA ALVLNASRRF 

        70         80         90        100        110        120 
RYTLDLKKEQ ETREMRQKIR SHAHALLAAN RFMDMGRESG VEKTTGPATP AGDFGITPEQ 

       130        140        150        160        170        180 
LVIMSKDHNS GALEQYGGTQ GLANLLKTNP EKGISGDDDD LLKRKTIYGS NTYPRKKGKG 

       190        200        210        220        230        240 
FLRFLWDACH DLTLIILMVA AVASLALGIK TEGIKEGWYD GGSIAFAVIL VIVVTAVSDY 

       250        260        270        280        290        300 
KQSLQFQNLN DEKRNIHLEV LRGGRRVEIS IYDIVVGDVI PLNIGNQVPA DGVLISGHSL 

       310        320        330        340        350        360 
ALDESSMTGE SKIVNKDANK DPFLMSGCKV ADGNGSMLVT GVGVNTEWGL LMASISEDNG 

       370        380        390        400        410        420 
EETPLQVRLN GVATFIGSIG LAVAAAVLVI LLTRYFTGHT KDNNGGPQFV KGKTKVGHVI 

       430        440        450        460        470        480 
DDVVKVLTVA VTIVVVAVPE GLPLAVTLTL AYSMRKMMAD KALVRRLSAC ETMGSATTIC 

       490        500        510        520        530        540 
SDKTGTLTLN QMTVVESYAG GKKTDTEQLP ATITSLVVEG ISQNTTGSIF VPEGGGDLEY 

       550        560        570        580        590        600 
SGSPTEKAIL GWGVKLGMNF ETARSQSSIL HAFPFNSEKK RGGVAVKTAD GEVHVHWKGA 

       610        620        630        640        650        660 
SEIVLASCRS YIDEDGNVAP MTDDKASFFK NGINDMAGRT LRCVALAFRT YEAEKVPTGE 

       670        680        690        700        710        720 
ELSKWVLPED DLILLAIVGI KDPCRPGVKD SVVLCQNAGV KVRMVTGDNV QTARAIALEC 

       730        740        750        760        770        780 
GILSSDADLS EPTLIEGKSF REMTDAERDK ISDKISVMGR SSPNDKLLLV QSLRRQGHVV 

       790        800        810        820        830        840 
AVTGDGTNDA PALHEADIGL AMGIAGTEVA KESSDIIILD DNFASVVKVV RWGRSVYANI 

       850        860        870        880        890        900 
QKFIQFQLTV NVAALVINVV AAISSGDVPL TAVQLLWVNL IMDTLGALAL ATEPPTDHLM 

       910        920        930        940        950        960 
GRPPVGRKEP LITNIMWRNL LIQAIYQVSV LLTLNFRGIS ILGLEHEVHE HATRVKNTII 

       970        980        990       1000       1010       1020 
FNAFVLCQAF NEFNARKPDE KNIFKGVIKN RLFMGIIVIT LVLQVIIVEF LGKFASTTKL 

      1030       1040       1050       1060       1070 
NWKQWLICVG IGVISWPLAL VGKFIPVPAA PISNKLKVLK FWGKKKNSSG EGSL 

« Hide

References

« Hide 'large scale' references
[1]"At-ACA8 encodes a plasma membrane-localized Ca2+-ATPase of Arabidopsis with a calmodulin-binding domain at the N-terminus."
Bonza M.C., Morandini P., Luoni L., Geisler M., Palmgren M.G., De Michelis M.I.
Plant Physiol. 123:1495-1506(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, CALMODULIN-BINDING DOMAIN.
Strain: cv. Landsberg erecta.
[2]"Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones."
Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S.
DNA Res. 7:31-63(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"Phosphoproteomics of the Arabidopsis plasma membrane and a new phosphorylation site database."
Nuehse T.S., Stensballe A., Jensen O.N., Peck S.C.
Plant Cell 16:2394-2405(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[6]"Temporal analysis of sucrose-induced phosphorylation changes in plasma membrane proteins of Arabidopsis."
Niittylae T., Fuglsang A.T., Palmgren M.G., Frommer W.B., Schulze W.X.
Mol. Cell. Proteomics 6:1711-1726(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Seedling.
[7]"Phosphoproteomic analysis of nuclei-enriched fractions from Arabidopsis thaliana."
Jones A.M.E., MacLean D., Studholme D.J., Serna-Sanz A., Andreasson E., Rathjen J.P., Peck S.C.
J. Proteomics 72:439-451(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: cv. Columbia.
[8]"Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks."
Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A., Grossmann J., Gruissem W., Baginsky S.
Plant Physiol. 150:889-903(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ249352 mRNA. Translation: CAB96189.1.
AB023042 Genomic DNA. Translation: BAA97361.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED96847.1.
CP002688 Genomic DNA. Translation: AED96848.1.
AY069869 mRNA. Translation: AAL47426.1.
PIRT52654.
RefSeqNP_200521.3. NM_125093.5.
NP_851200.1. NM_180869.3.
UniGeneAt.9676.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2M73NMR-A43-67[»]
4AQRX-ray1.95D40-95[»]
ProteinModelPortalQ9LF79.
SMRQ9LF79. Positions 39-95, 137-913.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid21059. 1 interaction.
IntActQ9LF79. 1 interaction.

Protein family/group databases

TCDB3.A.3.2.10. the p-type atpase (p-atpase) superfamily.

Proteomic databases

PaxDbQ9LF79.
PRIDEQ9LF79.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G57110.1; AT5G57110.1; AT5G57110.
AT5G57110.2; AT5G57110.2; AT5G57110.
GeneID835815.
KEGGath:AT5G57110.

Organism-specific databases

TAIRAT5G57110.

Phylogenomic databases

eggNOGCOG0474.
HOGENOMHOG000265623.
InParanoidQ9LF79.
KOK01537.
OMANGINDMA.
PhylomeDBQ9LF79.

Enzyme and pathway databases

BioCycARA:AT5G57110-MONOMER.
ARA:GQT-808-MONOMER.

Gene expression databases

GenevestigatorQ9LF79.

Family and domain databases

Gene3D1.20.1110.10. 2 hits.
3.40.1110.10. 1 hit.
InterProIPR006408. ATPase_P-typ_Ca-transp_plasma.
IPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR024750. Ca_ATPase_N_dom.
IPR001757. Cation_transp_P_typ_ATPase.
IPR023214. HAD-like_dom.
[Graphical view]
PfamPF12515. CaATP_NAI. 1 hit.
PF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view]
PRINTSPR00119. CATATPASE.
PR00120. HATPASE.
SMARTSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMSSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsTIGR01517. ATPase-IIB_Ca. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameACA8_ARATH
AccessionPrimary (citable) accession number: Q9LF79
Secondary accession number(s): Q9LU75
Entry history
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: October 1, 2000
Last modified: June 11, 2014
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names