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Protein

Calcium-transporting ATPase 8, plasma membrane-type

Gene

ACA8

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol out of the cell.

Catalytic activityi

ATP + H2O + Ca2+(Side 1) = ADP + phosphate + Ca2+(Side 2).

Enzyme regulationi

Activated by calmodulin.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei4824-aspartylphosphate intermediateBy similarity1
Metal bindingi785MagnesiumBy similarity1
Metal bindingi789MagnesiumBy similarity1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • calcium-transporting ATPase activity Source: TAIR
  • calmodulin binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW
  • protein self-association Source: TAIR

GO - Biological processi

  • response to nematode Source: TAIR

Keywordsi

Molecular functionCalmodulin-binding, Hydrolase
Biological processCalcium transport, Ion transport, Transport
LigandATP-binding, Calcium, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT5G57110-MONOMER
BRENDAi3.6.3.8 399
ReactomeiR-ATH-418359 Reduction of cytosolic Ca++ levels
R-ATH-5578775 Ion homeostasis
R-ATH-936837 Ion transport by P-type ATPases

Protein family/group databases

TCDBi3.A.3.2.10 the p-type atpase (p-atpase) superfamily

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium-transporting ATPase 8, plasma membrane-type (EC:3.6.3.8)
Alternative name(s):
Ca(2+)-ATPase isoform 8
Gene namesi
Name:ACA8
Ordered Locus Names:At5g57110
ORF Names:MUL3.5
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G57110
TAIRilocus:2175579 AT5G57110

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 180CytoplasmicSequence analysisAdd BLAST180
Transmembranei181 – 201HelicalSequence analysisAdd BLAST21
Topological domaini202 – 219ExtracellularSequence analysisAdd BLAST18
Transmembranei220 – 240HelicalSequence analysisAdd BLAST21
Topological domaini241 – 369CytoplasmicSequence analysisAdd BLAST129
Transmembranei370 – 389HelicalSequence analysisAdd BLAST20
Topological domaini390 – 426ExtracellularSequence analysisAdd BLAST37
Transmembranei427 – 444HelicalSequence analysisAdd BLAST18
Topological domaini445 – 840CytoplasmicSequence analysisAdd BLAST396
Transmembranei841 – 859HelicalSequence analysisAdd BLAST19
Topological domaini860 – 870ExtracellularSequence analysisAdd BLAST11
Transmembranei871 – 891HelicalSequence analysisAdd BLAST21
Topological domaini892 – 911CytoplasmicSequence analysisAdd BLAST20
Transmembranei912 – 934HelicalSequence analysisAdd BLAST23
Topological domaini935 – 949ExtracellularSequence analysisAdd BLAST15
Transmembranei950 – 971HelicalSequence analysisAdd BLAST22
Topological domaini972 – 989CytoplasmicSequence analysisAdd BLAST18
Transmembranei990 – 1011HelicalSequence analysisAdd BLAST22
Topological domaini1012 – 1021ExtracellularSequence analysis10
Transmembranei1022 – 1043HelicalSequence analysisAdd BLAST22
Topological domaini1044 – 1074CytoplasmicSequence analysisAdd BLAST31

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000464141 – 1074Calcium-transporting ATPase 8, plasma membrane-typeAdd BLAST1074

Proteomic databases

PaxDbiQ9LF79
PRIDEiQ9LF79

PTM databases

iPTMnetiQ9LF79
SwissPalmiQ9LF79

Expressioni

Gene expression databases

ExpressionAtlasiQ9LF79 baseline and differential
GenevisibleiQ9LF79 AT

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
CALMP6215714EBI-980643,EBI-397403From Bos taurus.

GO - Molecular functioni

  • calmodulin binding Source: UniProtKB-KW
  • protein self-association Source: TAIR

Protein-protein interaction databases

BioGridi210592 interactors.
IntActiQ9LF79 1 interactor.
STRINGi3702.AT5G57110.1

Structurei

Secondary structure

11074
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi40 – 94Combined sources55

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2M73NMR-A43-67[»]
4AQRX-ray1.95D40-95[»]
ProteinModelPortaliQ9LF79
SMRiQ9LF79
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni43 – 54Interaction with calmodulinAdd BLAST12

Domaini

The N-terminus contains an autoinhibitory calmodulin-binding domain, which binds calmodulin in a calcium-dependent fashion.

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0204 Eukaryota
ENOG410XNNC LUCA
HOGENOMiHOG000265623
InParanoidiQ9LF79
KOiK01537
OMAiNGINDMA
OrthoDBiEOG093601NF
PhylomeDBiQ9LF79

Family and domain databases

Gene3Di3.40.1110.101 hit
3.40.50.10002 hits
InterProiView protein in InterPro
IPR006068 ATPase_P-typ_cation-transptr_C
IPR004014 ATPase_P-typ_cation-transptr_N
IPR023299 ATPase_P-typ_cyto_dom_N
IPR018303 ATPase_P-typ_P_site
IPR023298 ATPase_P-typ_TM_dom_sf
IPR008250 ATPase_P-typ_transduc_dom_A_sf
IPR024750 Ca_ATPase_N_dom
IPR036412 HAD-like_sf
IPR023214 HAD_sf
IPR006408 P-type_ATPase_IIB
IPR001757 P_typ_ATPase
PfamiView protein in Pfam
PF12515 CaATP_NAI, 1 hit
PF00689 Cation_ATPase_C, 1 hit
PF00690 Cation_ATPase_N, 1 hit
PRINTSiPR00120 HATPASE
SMARTiView protein in SMART
SM00831 Cation_ATPase_N, 1 hit
SUPFAMiSSF56784 SSF56784, 2 hits
SSF81653 SSF81653, 1 hit
SSF81660 SSF81660, 1 hit
SSF81665 SSF81665, 3 hits
TIGRFAMsiTIGR01517 ATPase-IIB_Ca, 1 hit
TIGR01494 ATPase_P-type, 2 hits
PROSITEiView protein in PROSITE
PS00154 ATPASE_E1_E2, 1 hit

Sequencei

Sequence statusi: Complete.

Q9LF79-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSLLKSSPG RRRGGDVESG KSEHADSDSD TFYIPSKNAS IERLQQWRKA
60 70 80 90 100
ALVLNASRRF RYTLDLKKEQ ETREMRQKIR SHAHALLAAN RFMDMGRESG
110 120 130 140 150
VEKTTGPATP AGDFGITPEQ LVIMSKDHNS GALEQYGGTQ GLANLLKTNP
160 170 180 190 200
EKGISGDDDD LLKRKTIYGS NTYPRKKGKG FLRFLWDACH DLTLIILMVA
210 220 230 240 250
AVASLALGIK TEGIKEGWYD GGSIAFAVIL VIVVTAVSDY KQSLQFQNLN
260 270 280 290 300
DEKRNIHLEV LRGGRRVEIS IYDIVVGDVI PLNIGNQVPA DGVLISGHSL
310 320 330 340 350
ALDESSMTGE SKIVNKDANK DPFLMSGCKV ADGNGSMLVT GVGVNTEWGL
360 370 380 390 400
LMASISEDNG EETPLQVRLN GVATFIGSIG LAVAAAVLVI LLTRYFTGHT
410 420 430 440 450
KDNNGGPQFV KGKTKVGHVI DDVVKVLTVA VTIVVVAVPE GLPLAVTLTL
460 470 480 490 500
AYSMRKMMAD KALVRRLSAC ETMGSATTIC SDKTGTLTLN QMTVVESYAG
510 520 530 540 550
GKKTDTEQLP ATITSLVVEG ISQNTTGSIF VPEGGGDLEY SGSPTEKAIL
560 570 580 590 600
GWGVKLGMNF ETARSQSSIL HAFPFNSEKK RGGVAVKTAD GEVHVHWKGA
610 620 630 640 650
SEIVLASCRS YIDEDGNVAP MTDDKASFFK NGINDMAGRT LRCVALAFRT
660 670 680 690 700
YEAEKVPTGE ELSKWVLPED DLILLAIVGI KDPCRPGVKD SVVLCQNAGV
710 720 730 740 750
KVRMVTGDNV QTARAIALEC GILSSDADLS EPTLIEGKSF REMTDAERDK
760 770 780 790 800
ISDKISVMGR SSPNDKLLLV QSLRRQGHVV AVTGDGTNDA PALHEADIGL
810 820 830 840 850
AMGIAGTEVA KESSDIIILD DNFASVVKVV RWGRSVYANI QKFIQFQLTV
860 870 880 890 900
NVAALVINVV AAISSGDVPL TAVQLLWVNL IMDTLGALAL ATEPPTDHLM
910 920 930 940 950
GRPPVGRKEP LITNIMWRNL LIQAIYQVSV LLTLNFRGIS ILGLEHEVHE
960 970 980 990 1000
HATRVKNTII FNAFVLCQAF NEFNARKPDE KNIFKGVIKN RLFMGIIVIT
1010 1020 1030 1040 1050
LVLQVIIVEF LGKFASTTKL NWKQWLICVG IGVISWPLAL VGKFIPVPAA
1060 1070
PISNKLKVLK FWGKKKNSSG EGSL
Length:1,074
Mass (Da):116,174
Last modified:October 1, 2000 - v1
Checksum:i2E86572F44DEA8A5
GO

Sequence cautioni

The sequence BAA97361 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ249352 mRNA Translation: CAB96189.1
AB023042 Genomic DNA Translation: BAA97361.1 Sequence problems.
CP002688 Genomic DNA Translation: AED96847.1
CP002688 Genomic DNA Translation: AED96848.1
CP002688 Genomic DNA Translation: ANM69618.1
AY069869 mRNA Translation: AAL47426.1
PIRiT52654
RefSeqiNP_001331281.1, NM_001345242.1
NP_200521.3, NM_125093.6
NP_851200.1, NM_180869.3
UniGeneiAt.9676

Genome annotation databases

EnsemblPlantsiAT5G57110.1; AT5G57110.1; AT5G57110
AT5G57110.2; AT5G57110.2; AT5G57110
AT5G57110.3; AT5G57110.3; AT5G57110
GeneIDi835815
GrameneiAT5G57110.1; AT5G57110.1; AT5G57110
AT5G57110.2; AT5G57110.2; AT5G57110
AT5G57110.3; AT5G57110.3; AT5G57110
KEGGiath:AT5G57110

Similar proteinsi

Entry informationi

Entry nameiACA8_ARATH
AccessioniPrimary (citable) accession number: Q9LF79
Secondary accession number(s): Q9LU75
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: October 1, 2000
Last modified: April 25, 2018
This is version 163 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome