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Q9LF79

- ACA8_ARATH

UniProt

Q9LF79 - ACA8_ARATH

Protein

Calcium-transporting ATPase 8, plasma membrane-type

Gene

ACA8

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 131 (01 Oct 2014)
      Sequence version 1 (01 Oct 2000)
      Previous versions | rss
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    Functioni

    This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol out of the cell.

    Catalytic activityi

    ATP + H2O + Ca2+(Side 1) = ADP + phosphate + Ca2+(Side 2).

    Enzyme regulationi

    Activated by calmodulin.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei482 – 48214-aspartylphosphate intermediateBy similarity
    Metal bindingi785 – 7851MagnesiumBy similarity
    Metal bindingi789 – 7891MagnesiumBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. calcium-transporting ATPase activity Source: TAIR
    3. metal ion binding Source: UniProtKB-KW
    4. protein binding Source: IntAct
    5. protein self-association Source: TAIR

    GO - Biological processi

    1. response to nematode Source: TAIR

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Calcium transport, Ion transport, Transport

    Keywords - Ligandi

    ATP-binding, Calcium, Calmodulin-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:AT5G57110-MONOMER.
    ARA:GQT-808-MONOMER.

    Protein family/group databases

    TCDBi3.A.3.2.10. the p-type atpase (p-atpase) superfamily.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Calcium-transporting ATPase 8, plasma membrane-type (EC:3.6.3.8)
    Alternative name(s):
    Ca(2+)-ATPase isoform 8
    Gene namesi
    Name:ACA8
    Ordered Locus Names:At5g57110
    ORF Names:MUL3.5
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G57110.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. membrane Source: TAIR
    3. plasma membrane Source: TAIR
    4. plasmodesma Source: TAIR

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10741074Calcium-transporting ATPase 8, plasma membrane-typePRO_0000046414Add
    BLAST

    Proteomic databases

    PaxDbiQ9LF79.
    PRIDEiQ9LF79.

    Expressioni

    Gene expression databases

    GenevestigatoriQ9LF79.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    CALMP6215714EBI-980643,EBI-397403From a different organism.

    Protein-protein interaction databases

    BioGridi21059. 1 interaction.
    IntActiQ9LF79. 1 interaction.

    Structurei

    Secondary structure

    1
    1074
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi40 – 9455

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2M73NMR-A43-67[»]
    4AQRX-ray1.95D40-95[»]
    ProteinModelPortaliQ9LF79.
    SMRiQ9LF79. Positions 39-95, 137-913.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 180180CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini202 – 21918ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini241 – 369129CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini390 – 42637ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini445 – 840396CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini860 – 87011ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini892 – 91120CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini935 – 94915ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini972 – 98918CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini1012 – 102110ExtracellularSequence Analysis
    Topological domaini1044 – 107431CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei181 – 20121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei220 – 24021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei370 – 38920HelicalSequence AnalysisAdd
    BLAST
    Transmembranei427 – 44418HelicalSequence AnalysisAdd
    BLAST
    Transmembranei841 – 85919HelicalSequence AnalysisAdd
    BLAST
    Transmembranei871 – 89121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei912 – 93423HelicalSequence AnalysisAdd
    BLAST
    Transmembranei950 – 97122HelicalSequence AnalysisAdd
    BLAST
    Transmembranei990 – 101122HelicalSequence AnalysisAdd
    BLAST
    Transmembranei1022 – 104322HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni43 – 5412Interaction with calmodulinAdd
    BLAST

    Domaini

    The N-terminus contains an autoinhibitory calmodulin-binding domain, which binds calmodulin in a calcium-dependent fashion.

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0474.
    HOGENOMiHOG000265623.
    InParanoidiQ9LF79.
    KOiK01537.
    OMAiNGINDMA.
    PhylomeDBiQ9LF79.

    Family and domain databases

    Gene3Di1.20.1110.10. 2 hits.
    3.40.1110.10. 1 hit.
    InterProiIPR006408. ATPase_P-typ_Ca-transp_plasma.
    IPR006068. ATPase_P-typ_cation-transptr_C.
    IPR004014. ATPase_P-typ_cation-transptr_N.
    IPR023299. ATPase_P-typ_cyto_domN.
    IPR018303. ATPase_P-typ_P_site.
    IPR023298. ATPase_P-typ_TM_dom.
    IPR008250. ATPase_P-typ_transduc_dom_A.
    IPR024750. Ca_ATPase_N_dom.
    IPR001757. Cation_transp_P_typ_ATPase.
    IPR023214. HAD-like_dom.
    [Graphical view]
    PfamiPF12515. CaATP_NAI. 1 hit.
    PF00689. Cation_ATPase_C. 1 hit.
    PF00690. Cation_ATPase_N. 1 hit.
    PF00122. E1-E2_ATPase. 1 hit.
    PF00702. Hydrolase. 1 hit.
    [Graphical view]
    PRINTSiPR00119. CATATPASE.
    PR00120. HATPASE.
    SMARTiSM00831. Cation_ATPase_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF56784. SSF56784. 2 hits.
    SSF81660. SSF81660. 1 hit.
    TIGRFAMsiTIGR01517. ATPase-IIB_Ca. 1 hit.
    TIGR01494. ATPase_P-type. 2 hits.
    PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9LF79-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTSLLKSSPG RRRGGDVESG KSEHADSDSD TFYIPSKNAS IERLQQWRKA     50
    ALVLNASRRF RYTLDLKKEQ ETREMRQKIR SHAHALLAAN RFMDMGRESG 100
    VEKTTGPATP AGDFGITPEQ LVIMSKDHNS GALEQYGGTQ GLANLLKTNP 150
    EKGISGDDDD LLKRKTIYGS NTYPRKKGKG FLRFLWDACH DLTLIILMVA 200
    AVASLALGIK TEGIKEGWYD GGSIAFAVIL VIVVTAVSDY KQSLQFQNLN 250
    DEKRNIHLEV LRGGRRVEIS IYDIVVGDVI PLNIGNQVPA DGVLISGHSL 300
    ALDESSMTGE SKIVNKDANK DPFLMSGCKV ADGNGSMLVT GVGVNTEWGL 350
    LMASISEDNG EETPLQVRLN GVATFIGSIG LAVAAAVLVI LLTRYFTGHT 400
    KDNNGGPQFV KGKTKVGHVI DDVVKVLTVA VTIVVVAVPE GLPLAVTLTL 450
    AYSMRKMMAD KALVRRLSAC ETMGSATTIC SDKTGTLTLN QMTVVESYAG 500
    GKKTDTEQLP ATITSLVVEG ISQNTTGSIF VPEGGGDLEY SGSPTEKAIL 550
    GWGVKLGMNF ETARSQSSIL HAFPFNSEKK RGGVAVKTAD GEVHVHWKGA 600
    SEIVLASCRS YIDEDGNVAP MTDDKASFFK NGINDMAGRT LRCVALAFRT 650
    YEAEKVPTGE ELSKWVLPED DLILLAIVGI KDPCRPGVKD SVVLCQNAGV 700
    KVRMVTGDNV QTARAIALEC GILSSDADLS EPTLIEGKSF REMTDAERDK 750
    ISDKISVMGR SSPNDKLLLV QSLRRQGHVV AVTGDGTNDA PALHEADIGL 800
    AMGIAGTEVA KESSDIIILD DNFASVVKVV RWGRSVYANI QKFIQFQLTV 850
    NVAALVINVV AAISSGDVPL TAVQLLWVNL IMDTLGALAL ATEPPTDHLM 900
    GRPPVGRKEP LITNIMWRNL LIQAIYQVSV LLTLNFRGIS ILGLEHEVHE 950
    HATRVKNTII FNAFVLCQAF NEFNARKPDE KNIFKGVIKN RLFMGIIVIT 1000
    LVLQVIIVEF LGKFASTTKL NWKQWLICVG IGVISWPLAL VGKFIPVPAA 1050
    PISNKLKVLK FWGKKKNSSG EGSL 1074
    Length:1,074
    Mass (Da):116,174
    Last modified:October 1, 2000 - v1
    Checksum:i2E86572F44DEA8A5
    GO

    Sequence cautioni

    The sequence BAA97361.1 differs from that shown. Reason: Erroneous gene model prediction.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ249352 mRNA. Translation: CAB96189.1.
    AB023042 Genomic DNA. Translation: BAA97361.1. Sequence problems.
    CP002688 Genomic DNA. Translation: AED96847.1.
    CP002688 Genomic DNA. Translation: AED96848.1.
    AY069869 mRNA. Translation: AAL47426.1.
    PIRiT52654.
    RefSeqiNP_200521.3. NM_125093.5.
    NP_851200.1. NM_180869.3.
    UniGeneiAt.9676.

    Genome annotation databases

    EnsemblPlantsiAT5G57110.1; AT5G57110.1; AT5G57110.
    AT5G57110.2; AT5G57110.2; AT5G57110.
    GeneIDi835815.
    KEGGiath:AT5G57110.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ249352 mRNA. Translation: CAB96189.1 .
    AB023042 Genomic DNA. Translation: BAA97361.1 . Sequence problems.
    CP002688 Genomic DNA. Translation: AED96847.1 .
    CP002688 Genomic DNA. Translation: AED96848.1 .
    AY069869 mRNA. Translation: AAL47426.1 .
    PIRi T52654.
    RefSeqi NP_200521.3. NM_125093.5.
    NP_851200.1. NM_180869.3.
    UniGenei At.9676.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2M73 NMR - A 43-67 [» ]
    4AQR X-ray 1.95 D 40-95 [» ]
    ProteinModelPortali Q9LF79.
    SMRi Q9LF79. Positions 39-95, 137-913.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 21059. 1 interaction.
    IntActi Q9LF79. 1 interaction.

    Protein family/group databases

    TCDBi 3.A.3.2.10. the p-type atpase (p-atpase) superfamily.

    Proteomic databases

    PaxDbi Q9LF79.
    PRIDEi Q9LF79.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G57110.1 ; AT5G57110.1 ; AT5G57110 .
    AT5G57110.2 ; AT5G57110.2 ; AT5G57110 .
    GeneIDi 835815.
    KEGGi ath:AT5G57110.

    Organism-specific databases

    TAIRi AT5G57110.

    Phylogenomic databases

    eggNOGi COG0474.
    HOGENOMi HOG000265623.
    InParanoidi Q9LF79.
    KOi K01537.
    OMAi NGINDMA.
    PhylomeDBi Q9LF79.

    Enzyme and pathway databases

    BioCyci ARA:AT5G57110-MONOMER.
    ARA:GQT-808-MONOMER.

    Gene expression databases

    Genevestigatori Q9LF79.

    Family and domain databases

    Gene3Di 1.20.1110.10. 2 hits.
    3.40.1110.10. 1 hit.
    InterProi IPR006408. ATPase_P-typ_Ca-transp_plasma.
    IPR006068. ATPase_P-typ_cation-transptr_C.
    IPR004014. ATPase_P-typ_cation-transptr_N.
    IPR023299. ATPase_P-typ_cyto_domN.
    IPR018303. ATPase_P-typ_P_site.
    IPR023298. ATPase_P-typ_TM_dom.
    IPR008250. ATPase_P-typ_transduc_dom_A.
    IPR024750. Ca_ATPase_N_dom.
    IPR001757. Cation_transp_P_typ_ATPase.
    IPR023214. HAD-like_dom.
    [Graphical view ]
    Pfami PF12515. CaATP_NAI. 1 hit.
    PF00689. Cation_ATPase_C. 1 hit.
    PF00690. Cation_ATPase_N. 1 hit.
    PF00122. E1-E2_ATPase. 1 hit.
    PF00702. Hydrolase. 1 hit.
    [Graphical view ]
    PRINTSi PR00119. CATATPASE.
    PR00120. HATPASE.
    SMARTi SM00831. Cation_ATPase_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56784. SSF56784. 2 hits.
    SSF81660. SSF81660. 1 hit.
    TIGRFAMsi TIGR01517. ATPase-IIB_Ca. 1 hit.
    TIGR01494. ATPase_P-type. 2 hits.
    PROSITEi PS00154. ATPASE_E1_E2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "At-ACA8 encodes a plasma membrane-localized Ca2+-ATPase of Arabidopsis with a calmodulin-binding domain at the N-terminus."
      Bonza M.C., Morandini P., Luoni L., Geisler M., Palmgren M.G., De Michelis M.I.
      Plant Physiol. 123:1495-1506(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, CALMODULIN-BINDING DOMAIN.
      Strain: cv. Landsberg erecta.
    2. "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones."
      Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S.
      DNA Res. 7:31-63(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Phosphoproteomics of the Arabidopsis plasma membrane and a new phosphorylation site database."
      Nuehse T.S., Stensballe A., Jensen O.N., Peck S.C.
      Plant Cell 16:2394-2405(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    6. "Temporal analysis of sucrose-induced phosphorylation changes in plasma membrane proteins of Arabidopsis."
      Niittylae T., Fuglsang A.T., Palmgren M.G., Frommer W.B., Schulze W.X.
      Mol. Cell. Proteomics 6:1711-1726(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Seedling.
    7. "Phosphoproteomic analysis of nuclei-enriched fractions from Arabidopsis thaliana."
      Jones A.M.E., MacLean D., Studholme D.J., Serna-Sanz A., Andreasson E., Rathjen J.P., Peck S.C.
      J. Proteomics 72:439-451(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: cv. Columbia.
    8. "Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks."
      Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A., Grossmann J., Gruissem W., Baginsky S.
      Plant Physiol. 150:889-903(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiACA8_ARATH
    AccessioniPrimary (citable) accession number: Q9LF79
    Secondary accession number(s): Q9LU75
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 11, 2001
    Last sequence update: October 1, 2000
    Last modified: October 1, 2014
    This is version 131 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3