Calcium-transporting ATPase 8, plasma membrane-type - Arabidopsis thaliana (Mouse-ear cress)

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Reviewed, UniProtKB/Swiss-Prot Q9LF79 (ACA8_ARATH)

Last modified June 16, 2009. Version 86. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Calcium-transporting ATPase 8, plasma membrane-type
    EC=3.6.3.8
Alternative name(s):
    Ca(2+)-ATPase isoform 8

Gene names

Name:	ACA8
Ordered Locus Names:	At5g57110
ORF Names:	MUL3.5

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

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Protein attributes

Sequence length	1074 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol out of the cell.
Catalytic activity	ATP + H₂O + Ca²⁺(Cis) = ADP + phosphate + Ca²⁺(Trans).
Enzyme regulation	Activated by calmodulin By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Domain	The N-terminus contains an autoinhibitory calmodulin-binding domain, which binds calmodulin in a calcium-dependent fashion. Ref.1
Sequence similarities	Belongs to the cation transport ATPase (P-type) family. Type IIB subfamily.
Sequence caution	The sequence BAA97361.1 differs from that shown. Reason: Erroneous gene model prediction.

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Ontologies

Keywords
Biological process	Calcium transport Ion transport Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Ligand	ATP-binding Calcium Calmodulin-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	ATP biosynthetic process Inferred from electronic annotation. Source: InterPro calcium ion transport Inferred from electronic annotation. Source: UniProtKB-KW response to nematode Inferred from expression pattern. Source: TAIR
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Ref.1 Inferred from direct assay. Source: TAIR
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW calcium-transporting ATPase activity Inferred from direct assay. Source: TAIR calmodulin binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein self-association Inferred from direct assay. Source: TAIR
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct	Notes
CALM	P62157	2	EBI-980643,EBI-397403	From a different organism.

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1074

1074

Calcium-transporting ATPase 8, plasma membrane-type

PRO_0000046414

Regions

Topological domain

1 – 180

180

Cytoplasmic Potential

Transmembrane

181 – 201

Potential

Topological domain

202 – 219

Extracellular Potential

Transmembrane

220 – 240

Potential

Topological domain

241 – 369

129

Cytoplasmic Potential

Transmembrane

370 – 389

Potential

Topological domain

390 – 426

Extracellular Potential

Transmembrane

427 – 444

Potential

Topological domain

445 – 840

396

Cytoplasmic Potential

Transmembrane

841 – 859

Potential

Topological domain

860 – 870

Extracellular Potential

Transmembrane

871 – 891

Potential

Topological domain

892 – 911

Cytoplasmic Potential

Transmembrane

912 – 934

Potential

Topological domain

935 – 949

Extracellular Potential

Transmembrane

950 – 971

Potential

Topological domain

972 – 989

Cytoplasmic Potential

Transmembrane

990 – 1011

Potential

Topological domain

1012 – 1021

Extracellular Potential

Transmembrane

1022 – 1043

Potential

Topological domain

1044 – 1074

Cytoplasmic Potential

Region

43 – 54

Interaction with calmodulin

Sites

Active site

482

4-aspartylphosphate intermediate By similarity

Metal binding

785

Magnesium By similarity

Metal binding

789

Magnesium By similarity

Amino acid modifications

Modified residue

Phosphoserine Ref.4

Modified residue

Phosphoserine Ref.4 Ref.5

Modified residue

Phosphoserine Ref.4

Modified residue

Phosphoserine Ref.4

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Sequences

Sequence

Length

Mass (Da)

Tools

Q9LF79-1 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 2E86572F44DEA8A5
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FASTA

1,074

116,174

        10         20         30         40         50         60 
MTSLLKSSPG RRRGGDVESG KSEHADSDSD TFYIPSKNAS IERLQQWRKA ALVLNASRRF 

        70         80         90        100        110        120 
RYTLDLKKEQ ETREMRQKIR SHAHALLAAN RFMDMGRESG VEKTTGPATP AGDFGITPEQ 

       130        140        150        160        170        180 
LVIMSKDHNS GALEQYGGTQ GLANLLKTNP EKGISGDDDD LLKRKTIYGS NTYPRKKGKG 

       190        200        210        220        230        240 
FLRFLWDACH DLTLIILMVA AVASLALGIK TEGIKEGWYD GGSIAFAVIL VIVVTAVSDY 

       250        260        270        280        290        300 
KQSLQFQNLN DEKRNIHLEV LRGGRRVEIS IYDIVVGDVI PLNIGNQVPA DGVLISGHSL 

       310        320        330        340        350        360 
ALDESSMTGE SKIVNKDANK DPFLMSGCKV ADGNGSMLVT GVGVNTEWGL LMASISEDNG 

       370        380        390        400        410        420 
EETPLQVRLN GVATFIGSIG LAVAAAVLVI LLTRYFTGHT KDNNGGPQFV KGKTKVGHVI 

       430        440        450        460        470        480 
DDVVKVLTVA VTIVVVAVPE GLPLAVTLTL AYSMRKMMAD KALVRRLSAC ETMGSATTIC 

       490        500        510        520        530        540 
SDKTGTLTLN QMTVVESYAG GKKTDTEQLP ATITSLVVEG ISQNTTGSIF VPEGGGDLEY 

       550        560        570        580        590        600 
SGSPTEKAIL GWGVKLGMNF ETARSQSSIL HAFPFNSEKK RGGVAVKTAD GEVHVHWKGA 

       610        620        630        640        650        660 
SEIVLASCRS YIDEDGNVAP MTDDKASFFK NGINDMAGRT LRCVALAFRT YEAEKVPTGE 

       670        680        690        700        710        720 
ELSKWVLPED DLILLAIVGI KDPCRPGVKD SVVLCQNAGV KVRMVTGDNV QTARAIALEC 

       730        740        750        760        770        780 
GILSSDADLS EPTLIEGKSF REMTDAERDK ISDKISVMGR SSPNDKLLLV QSLRRQGHVV 

       790        800        810        820        830        840 
AVTGDGTNDA PALHEADIGL AMGIAGTEVA KESSDIIILD DNFASVVKVV RWGRSVYANI 

       850        860        870        880        890        900 
QKFIQFQLTV NVAALVINVV AAISSGDVPL TAVQLLWVNL IMDTLGALAL ATEPPTDHLM 

       910        920        930        940        950        960 
GRPPVGRKEP LITNIMWRNL LIQAIYQVSV LLTLNFRGIS ILGLEHEVHE HATRVKNTII 

       970        980        990       1000       1010       1020 
FNAFVLCQAF NEFNARKPDE KNIFKGVIKN RLFMGIIVIT LVLQVIIVEF LGKFASTTKL 

      1030       1040       1050       1060       1070 
NWKQWLICVG IGVISWPLAL VGKFIPVPAA PISNKLKVLK FWGKKKNSSG EGSL

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"At-ACA8 encodes a plasma membrane-localized Ca2+-ATPase of Arabidopsis with a calmodulin-binding domain at the N-terminus." Bonza M.C., Morandini P., Luoni L., Geisler M., Palmgren M.G., De Michelis M.I. Plant Physiol. 123:1495-1506(2000) [PubMed: 10938365] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, CALMODULIN-BINDING DOMAIN. Strain: cv. Landsberg erecta.
[2]	"Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones." Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S. DNA Res. 7:31-63(2000) [PubMed: 10718197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Phosphoproteomics of the Arabidopsis plasma membrane and a new phosphorylation site database." Nuehse T.S., Stensballe A., Jensen O.N., Peck S.C. Plant Cell 16:2394-2405(2004) [PubMed: 15308754] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-19; SER-22; SER-27 AND SER-29, MASS SPECTROMETRY.
[5]	"Temporal analysis of sucrose-induced phosphorylation changes in plasma membrane proteins of Arabidopsis." Niittylae T., Fuglsang A.T., Palmgren M.G., Frommer W.B., Schulze W.X. Mol. Cell. Proteomics 6:1711-1726(2007) [PubMed: 17586839] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-22, MASS SPECTROMETRY.

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Cross-references

Sequence databases
	AJ249352 mRNA. Translation: CAB96189.1. AB023042 Genomic DNA. Translation: BAA97361.1. Sequence problems. AY069869 mRNA. Translation: AAL47426.1.
IPI	IPI00540817.
PIR	T52654.
RefSeq	NP_200521.3. NP_851200.1.
UniGene	At.9676
3D structure databases
HSSP	HSSP built from PDB template 1EUL based on UniProtKB P04191.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9LF79. 1 interaction.
Protein family/group databases
TCDB	3.A.3.2.10. P-type ATPase (P-ATPase) superfamily.
Proteomic databases
PRIDE	Q9LF79.
Genome annotation databases
GeneID	835815.
GenomeReviews	Gene locus AT5G57110 in contig BA000015_GR.
KEGG	ath:AT5G57110.
NMPDR	fig\|3702.1.peg.27655.
Organism-specific databases
TAIR	At5g57110.
Phylogenomic databases
OMA	Q9LF79. GISTHEP.
Enzyme and pathway databases
BRENDA	3.6.3.8. 302.
Gene expression databases
ArrayExpress	Q9LF79.
GermOnline	AT5G57110. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR008250. ATPase_P-typ_ATPase-assoc-reg. IPR006408. ATPase_P-typ_Ca-transp_PMCA. IPR006068. ATPase_P-typ_cation-transptr_C. IPR004014. ATPase_P-typ_cation-transptr_N. IPR000695. ATPase_P-typ_H-transp. IPR001757. ATPase_P-typ_ion-transptr. IPR018303. ATPase_P-typ_phosphor_site. IPR005834. Dehalogen-like_hydro. [Graphical view]
PANTHER	PTHR11939. ATPase_P. 1 hit.
Pfam	PF00689. Cation_ATPase_C. 1 hit. PF00690. Cation_ATPase_N. 1 hit. PF00122. E1-E2_ATPase. 1 hit. PF00702. Hydrolase. 1 hit. [Graphical view]
PRINTS	PR00119. CATATPASE. PR00120. HATPASE.
TIGRFAMs	TIGR01517. ATPase-IIB_Ca. 1 hit. TIGR01494. ATPase_P-type. 3 hits.
PROSITE	PS00154. ATPASE_E1_E2. 1 hit. [Graphical view]
ProtoNet	Search...

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Entry information

Entry name

ACA8_ARATH

Accession

Primary (citable) accession number: Q9LF79
Secondary accession number(s): Q9LU75

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	October 1, 2000
Last modified:	June 16, 2009
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)

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Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents