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Q9LF79

- ACA8_ARATH

UniProt

Q9LF79 - ACA8_ARATH

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Protein

Calcium-transporting ATPase 8, plasma membrane-type

Gene
ACA8, At5g57110, MUL3.5
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol out of the cell.

Catalytic activityi

ATP + H2O + Ca2+(Side 1) = ADP + phosphate + Ca2+(Side 2).

Enzyme regulationi

Activated by calmodulin By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei482 – 48214-aspartylphosphate intermediate By similarity
Metal bindingi785 – 7851Magnesium By similarity
Metal bindingi789 – 7891Magnesium By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. calcium-transporting ATPase activity Source: TAIR
  3. metal ion binding Source: UniProtKB-KW
  4. protein binding Source: IntAct
  5. protein self-association Source: TAIR

GO - Biological processi

  1. response to nematode Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Calcium transport, Ion transport, Transport

Keywords - Ligandi

ATP-binding, Calcium, Calmodulin-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT5G57110-MONOMER.
ARA:GQT-808-MONOMER.

Protein family/group databases

TCDBi3.A.3.2.10. the p-type atpase (p-atpase) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium-transporting ATPase 8, plasma membrane-type (EC:3.6.3.8)
Alternative name(s):
Ca(2+)-ATPase isoform 8
Gene namesi
Name:ACA8
Ordered Locus Names:At5g57110
ORF Names:MUL3.5
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 5

Organism-specific databases

TAIRiAT5G57110.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 180180Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei181 – 20121Helical; Reviewed predictionAdd
BLAST
Topological domaini202 – 21918Extracellular Reviewed predictionAdd
BLAST
Transmembranei220 – 24021Helical; Reviewed predictionAdd
BLAST
Topological domaini241 – 369129Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei370 – 38920Helical; Reviewed predictionAdd
BLAST
Topological domaini390 – 42637Extracellular Reviewed predictionAdd
BLAST
Transmembranei427 – 44418Helical; Reviewed predictionAdd
BLAST
Topological domaini445 – 840396Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei841 – 85919Helical; Reviewed predictionAdd
BLAST
Topological domaini860 – 87011Extracellular Reviewed predictionAdd
BLAST
Transmembranei871 – 89121Helical; Reviewed predictionAdd
BLAST
Topological domaini892 – 91120Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei912 – 93423Helical; Reviewed predictionAdd
BLAST
Topological domaini935 – 94915Extracellular Reviewed predictionAdd
BLAST
Transmembranei950 – 97122Helical; Reviewed predictionAdd
BLAST
Topological domaini972 – 98918Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei990 – 101122Helical; Reviewed predictionAdd
BLAST
Topological domaini1012 – 102110Extracellular Reviewed prediction
Transmembranei1022 – 104322Helical; Reviewed predictionAdd
BLAST
Topological domaini1044 – 107431Cytoplasmic Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. membrane Source: TAIR
  3. plasma membrane Source: TAIR
  4. plasmodesma Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10741074Calcium-transporting ATPase 8, plasma membrane-typePRO_0000046414Add
BLAST

Proteomic databases

PaxDbiQ9LF79.
PRIDEiQ9LF79.

Expressioni

Gene expression databases

GenevestigatoriQ9LF79.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
CALMP6215714EBI-980643,EBI-397403From a different organism.

Protein-protein interaction databases

BioGridi21059. 1 interaction.
IntActiQ9LF79. 1 interaction.

Structurei

Secondary structure

1
1074
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi40 – 9455

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2M73NMR-A43-67[»]
4AQRX-ray1.95D40-95[»]
ProteinModelPortaliQ9LF79.
SMRiQ9LF79. Positions 39-95, 137-913.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni43 – 5412Interaction with calmodulinAdd
BLAST

Domaini

The N-terminus contains an autoinhibitory calmodulin-binding domain, which binds calmodulin in a calcium-dependent fashion.1 Publication

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0474.
HOGENOMiHOG000265623.
InParanoidiQ9LF79.
KOiK01537.
OMAiNGINDMA.
PhylomeDBiQ9LF79.

Family and domain databases

Gene3Di1.20.1110.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR006408. ATPase_P-typ_Ca-transp_plasma.
IPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR024750. Ca_ATPase_N_dom.
IPR001757. Cation_transp_P_typ_ATPase.
IPR023214. HAD-like_dom.
[Graphical view]
PfamiPF12515. CaATP_NAI. 1 hit.
PF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view]
PRINTSiPR00119. CATATPASE.
PR00120. HATPASE.
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01517. ATPase-IIB_Ca. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9LF79-1 [UniParc]FASTAAdd to Basket

« Hide

MTSLLKSSPG RRRGGDVESG KSEHADSDSD TFYIPSKNAS IERLQQWRKA     50
ALVLNASRRF RYTLDLKKEQ ETREMRQKIR SHAHALLAAN RFMDMGRESG 100
VEKTTGPATP AGDFGITPEQ LVIMSKDHNS GALEQYGGTQ GLANLLKTNP 150
EKGISGDDDD LLKRKTIYGS NTYPRKKGKG FLRFLWDACH DLTLIILMVA 200
AVASLALGIK TEGIKEGWYD GGSIAFAVIL VIVVTAVSDY KQSLQFQNLN 250
DEKRNIHLEV LRGGRRVEIS IYDIVVGDVI PLNIGNQVPA DGVLISGHSL 300
ALDESSMTGE SKIVNKDANK DPFLMSGCKV ADGNGSMLVT GVGVNTEWGL 350
LMASISEDNG EETPLQVRLN GVATFIGSIG LAVAAAVLVI LLTRYFTGHT 400
KDNNGGPQFV KGKTKVGHVI DDVVKVLTVA VTIVVVAVPE GLPLAVTLTL 450
AYSMRKMMAD KALVRRLSAC ETMGSATTIC SDKTGTLTLN QMTVVESYAG 500
GKKTDTEQLP ATITSLVVEG ISQNTTGSIF VPEGGGDLEY SGSPTEKAIL 550
GWGVKLGMNF ETARSQSSIL HAFPFNSEKK RGGVAVKTAD GEVHVHWKGA 600
SEIVLASCRS YIDEDGNVAP MTDDKASFFK NGINDMAGRT LRCVALAFRT 650
YEAEKVPTGE ELSKWVLPED DLILLAIVGI KDPCRPGVKD SVVLCQNAGV 700
KVRMVTGDNV QTARAIALEC GILSSDADLS EPTLIEGKSF REMTDAERDK 750
ISDKISVMGR SSPNDKLLLV QSLRRQGHVV AVTGDGTNDA PALHEADIGL 800
AMGIAGTEVA KESSDIIILD DNFASVVKVV RWGRSVYANI QKFIQFQLTV 850
NVAALVINVV AAISSGDVPL TAVQLLWVNL IMDTLGALAL ATEPPTDHLM 900
GRPPVGRKEP LITNIMWRNL LIQAIYQVSV LLTLNFRGIS ILGLEHEVHE 950
HATRVKNTII FNAFVLCQAF NEFNARKPDE KNIFKGVIKN RLFMGIIVIT 1000
LVLQVIIVEF LGKFASTTKL NWKQWLICVG IGVISWPLAL VGKFIPVPAA 1050
PISNKLKVLK FWGKKKNSSG EGSL 1074
Length:1,074
Mass (Da):116,174
Last modified:October 1, 2000 - v1
Checksum:i2E86572F44DEA8A5
GO

Sequence cautioni

The sequence BAA97361.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ249352 mRNA. Translation: CAB96189.1.
AB023042 Genomic DNA. Translation: BAA97361.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED96847.1.
CP002688 Genomic DNA. Translation: AED96848.1.
AY069869 mRNA. Translation: AAL47426.1.
PIRiT52654.
RefSeqiNP_200521.3. NM_125093.5.
NP_851200.1. NM_180869.3.
UniGeneiAt.9676.

Genome annotation databases

EnsemblPlantsiAT5G57110.1; AT5G57110.1; AT5G57110.
AT5G57110.2; AT5G57110.2; AT5G57110.
GeneIDi835815.
KEGGiath:AT5G57110.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ249352 mRNA. Translation: CAB96189.1 .
AB023042 Genomic DNA. Translation: BAA97361.1 . Sequence problems.
CP002688 Genomic DNA. Translation: AED96847.1 .
CP002688 Genomic DNA. Translation: AED96848.1 .
AY069869 mRNA. Translation: AAL47426.1 .
PIRi T52654.
RefSeqi NP_200521.3. NM_125093.5.
NP_851200.1. NM_180869.3.
UniGenei At.9676.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2M73 NMR - A 43-67 [» ]
4AQR X-ray 1.95 D 40-95 [» ]
ProteinModelPortali Q9LF79.
SMRi Q9LF79. Positions 39-95, 137-913.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 21059. 1 interaction.
IntActi Q9LF79. 1 interaction.

Protein family/group databases

TCDBi 3.A.3.2.10. the p-type atpase (p-atpase) superfamily.

Proteomic databases

PaxDbi Q9LF79.
PRIDEi Q9LF79.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT5G57110.1 ; AT5G57110.1 ; AT5G57110 .
AT5G57110.2 ; AT5G57110.2 ; AT5G57110 .
GeneIDi 835815.
KEGGi ath:AT5G57110.

Organism-specific databases

TAIRi AT5G57110.

Phylogenomic databases

eggNOGi COG0474.
HOGENOMi HOG000265623.
InParanoidi Q9LF79.
KOi K01537.
OMAi NGINDMA.
PhylomeDBi Q9LF79.

Enzyme and pathway databases

BioCyci ARA:AT5G57110-MONOMER.
ARA:GQT-808-MONOMER.

Gene expression databases

Genevestigatori Q9LF79.

Family and domain databases

Gene3Di 1.20.1110.10. 2 hits.
3.40.1110.10. 1 hit.
InterProi IPR006408. ATPase_P-typ_Ca-transp_plasma.
IPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR024750. Ca_ATPase_N_dom.
IPR001757. Cation_transp_P_typ_ATPase.
IPR023214. HAD-like_dom.
[Graphical view ]
Pfami PF12515. CaATP_NAI. 1 hit.
PF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view ]
PRINTSi PR00119. CATATPASE.
PR00120. HATPASE.
SMARTi SM00831. Cation_ATPase_N. 1 hit.
[Graphical view ]
SUPFAMi SSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsi TIGR01517. ATPase-IIB_Ca. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEi PS00154. ATPASE_E1_E2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "At-ACA8 encodes a plasma membrane-localized Ca2+-ATPase of Arabidopsis with a calmodulin-binding domain at the N-terminus."
    Bonza M.C., Morandini P., Luoni L., Geisler M., Palmgren M.G., De Michelis M.I.
    Plant Physiol. 123:1495-1506(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, CALMODULIN-BINDING DOMAIN.
    Strain: cv. Landsberg erecta.
  2. "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones."
    Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S.
    DNA Res. 7:31-63(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Phosphoproteomics of the Arabidopsis plasma membrane and a new phosphorylation site database."
    Nuehse T.S., Stensballe A., Jensen O.N., Peck S.C.
    Plant Cell 16:2394-2405(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  6. "Temporal analysis of sucrose-induced phosphorylation changes in plasma membrane proteins of Arabidopsis."
    Niittylae T., Fuglsang A.T., Palmgren M.G., Frommer W.B., Schulze W.X.
    Mol. Cell. Proteomics 6:1711-1726(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Seedling.
  7. "Phosphoproteomic analysis of nuclei-enriched fractions from Arabidopsis thaliana."
    Jones A.M.E., MacLean D., Studholme D.J., Serna-Sanz A., Andreasson E., Rathjen J.P., Peck S.C.
    J. Proteomics 72:439-451(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: cv. Columbia.
  8. "Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks."
    Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A., Grossmann J., Gruissem W., Baginsky S.
    Plant Physiol. 150:889-903(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiACA8_ARATH
AccessioniPrimary (citable) accession number: Q9LF79
Secondary accession number(s): Q9LU75
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: October 1, 2000
Last modified: June 11, 2014
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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