Q9LEX0 (P2A13_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
F-box protein PP2-A13

Alternative name(s):
Protein PHLOEM PROTEIN 2-LIKE A13
Short name=AtPP2-A13
SKP1-interacting partner 9

Gene names

Name:	PP2A13
Synonyms:	SKIP9
Ordered Locus Names:	At3g61060
ORF Names:	T27I15.150

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	290 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of SCF(ASK-cullin-F-box) E3 ubiquitin ligase complexes, which may mediate the ubiquitination and subsequent proteasomal degradation of target proteins By similarity.
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Part of a SCF (ASK-cullin-F-box) protein ligase complex By similarity. Interacts with SKP1A/ASK1, SKP1B/ASK2, ASK5, ASK11 and ASK13. Ref.4 Ref.5
Subcellular location	Nucleus By similarity.
Domain	The F-box is necessary for the interaction with ASK proteins By similarity.
Sequence similarities	Contains 1 F-box domain.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	response to wounding Inferred from expression pattern. Source: TAIR
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Inferred from physical interaction Ref.4. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
SKP1A	Q39255	4	EBI-604261,EBI-532357
SKP1B	Q9FHW7	6	EBI-604261,EBI-604076

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q9LEX0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 290	290	F-box protein PP2-A13		PRO_0000272208
Regions
	Domain	21 – 67	47	F-box
Experimental info
	Sequence conflict	207	1	I → T in AAK59472. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: DCC145BB0D652EC9	FASTA	290	33,115
	10 20 30 40 50 60 MGANISGGSP EFDRNDDVYS RKLRLVDLPE NCVALIMTRL DPPEICRLAR LNRMFRRASS 70 80 90 100 110 120 ADFIWESKLP ANYRVIAHKV FDEITLTKLI KKDLYAKLSQ PNLFDDGTKE LWIDKNTGRL 130 140 150 160 170 180 CLSISSKALR ITGIDDRRYW SHIPTDESRF QSAAYVQQIW WFEVGGEFEI QFPSGTYSLF 190 200 210 220 230 240 FRIQLGKTSK RLGRRICNSE HIHGWDIKPV RFQLATSDNQ QAVSLCYLNN NPGSWSHYHV 250 260 270 280 290 GDFKVTNPDV STGIKFSMTQ IDCTHTKGGL CIDSVLILPK ECAKEVIGSQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana." Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. Nature 408:820-822(2000) [PubMed: 11130713] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"The F-box subunit of the SCF E3 complex is encoded by a diverse superfamily of genes in Arabidopsis." Gagne J.M., Downes B.P., Shiu S.-H., Durski A.M., Vierstra R.D. Proc. Natl. Acad. Sci. U.S.A. 99:11519-11524(2002) [PubMed: 12169662] [Abstract] Cited for: INTERACTION WITH SKP1A/ASK1; SKP1B/ASK2; ASK5; ASK11 AND ASK13.
[5]	"Protein interaction analysis of SCF ubiquitin E3 ligase subunits from Arabidopsis." Risseeuw E.P., Daskalchuk T.E., Banks T.W., Liu E., Cotelesage J., Hellmann H., Estelle M., Somers D.E., Crosby W.L. Plant J. 34:753-767(2003) [PubMed: 12795696] [Abstract] Cited for: INTERACTION WITH SKPIA/ASK1 AND SKP1B/ASK2.
[6]	"Diversity of the superfamily of phloem lectins (phloem protein 2) in angiosperms." Dinant S., Clark A.M., Zhu Y., Vilaine F., Palauqui J.-C., Kusiak C., Thompson G.A. Plant Physiol. 131:114-128(2003) [PubMed: 12529520] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
+	Additional computationally mapped references.

Web resources

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL358732 Genomic DNA. Translation: CAB94142.1. CP002686 Genomic DNA. Translation: AEE80147.1. AY034967 mRNA. Translation: AAK59472.2. BT000895 mRNA. Translation: AAN41295.1.
IPI	IPI00540042.
PIR	T50527.
RefSeq	NP_567108.2. NM_115970.3.
UniGene	At.44960. At.74763. At.74871.
3D structure databases
ProteinModelPortal	Q9LEX0.
SMR	Q9LEX0. Positions 25-68.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9LEX0. 8 interactions.
STRING	Q9LEX0.
Proteomic databases
PRIDE	Q9LEX0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT3G61060.1; AT3G61060.1; AT3G61060.
GeneID	825278.
GenomeReviews	Gene locus AT3G61060 in contig BA000014_GR.
KEGG	ath:AT3G61060.
NMPDR	fig|3702.1.peg.17476.
Organism-specific databases
TAIR	At3g61060.
Phylogenomic databases
GeneTree	EPGT00070000028246.
HOGENOM	HBG317035.
InParanoid	Q9LEX0.
PhylomeDB	Q9LEX0.
ProtClustDB	CLSN2682374.
Gene expression databases
Genevestigator	Q9LEX0.
Family and domain databases
InterPro	IPR001810. F-box_dom_cyclin-like. IPR022364. F-box_dom_Skp2-like. [Graphical view]
SMART	SM00256. FBOX. 1 hit. [Graphical view]
SUPFAM	SSF81383. F-box_dom_Skp2-like. 1 hit.
PROSITE	PS50181. FBOX. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

P2A13_ARATH

Accession

Primary (citable) accession number: Q9LEX0
Secondary accession number(s): Q3EAG4, Q94CC2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2007
Last sequence update:	October 1, 2000
Last modified:	December 14, 2011
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program