Q9LEW0 (UBP22_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin carboxyl-terminal hydrolase 22
EC=3.4.19.12

Alternative name(s):
Deubiquitinating enzyme 22
Short name=AtUBP22
Ubiquitin thioesterase 22
Ubiquitin-specific-processing protease 22

Gene names

Name:	UBP22
Ordered Locus Names:	At5g10790
ORF Names:	T30N20_60

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	557 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins By similarity.
Catalytic activity	Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Sequence similarities	Belongs to the peptidase C19 family. Contains 1 UBP-type zinc finger.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Protease Thiol protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	cysteine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin thiolesterase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 557	557	Ubiquitin carboxyl-terminal hydrolase 22		PRO_0000313048
Regions
	Zinc finger	54 – 113	60	UBP-type
Sites
	Active site	186	1	Nucleophile By similarity
	Active site	491	1	Proton acceptor By similarity
Experimental info
	Sequence conflict	111	1	V → A in AAG42760. Ref.1
	Sequence conflict	231	1	V → I in AAG42760. Ref.1
	Sequence conflict	416	1	S → F in AAG42760. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9LEW0 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 3CA6C313AF0FF82C	FASTA	557	63,518
	10 20 30 40 50 60 MSARISFLKN PDPCNHLSDY KLRYGTDGYK SFNNLFRCFN DARIKIKLQG IDIPRCSYCS 70 80 90 100 110 120 VYQKRLYICL ICRSISCSSH ILLHTQLNKG HDIAIDVERS ELYCCACIDQ VYDSEFDEVV 130 140 150 160 170 180 VSKQLFGLGM SVKSGADVVA VRSNKKRRLD SQLIIGSNFL VSPRDRREKW TFPLGLRGLN 190 200 210 220 230 240 NLGSTCFMNA VLQALVHAPP LRNFWLSGQH NRDLCPRRTM GLLCLPCDLD VIFSAMFSGD 250 260 270 280 290 300 RTPYSPAHLL YSWWQHSTNL ATYEQQDSHE FFISLLDRIH ENEGKSKCLY QDNEECQCIT 310 320 330 340 350 360 HKAFSGLLRS DVTCTTCGST STTYDPFIDI SLTLDSMNGF SPADCRKNRY SGGPSVNAIM 370 380 390 400 410 420 PTLSGCLDFF TRSEKLGPDQ KLNCQSCGEK RESSKQMSIR RLPLLLCLHV KRFEHSLTRK 430 440 450 460 470 480 TSRKIDSYLQ YPFRLNMSPY LSSSIIGKRF GNRIFAFDGE GEYDSSSSSS PSAEFEIFAV 490 500 510 520 530 540 VTHKGMLESG HYVTYLRLKG LWYRCDDAWI NEVEEEVVRG CECYMLFYAQ ETVIQKAHKE 550 LSYQVISMAD AFPFADC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The ubiquitin-specific protease family from Arabidopsis. AtUBP1 and 2 are required for the resistance to the amino acid analog canavanine." Yan N., Doelling J.H., Falbel T.G., Durski A.M., Vierstra R.D. Plant Physiol. 124:1828-1843(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY ORGANIZATION, NOMENCLATURE. Strain: cv. Columbia.
[2]	"Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana." Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K. , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F. Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF302670 mRNA. Translation: AAG42760.1. AL365234 Genomic DNA. Translation: CAB96834.1. CP002688 Genomic DNA. Translation: AED91597.1.
IPI	IPI00533926.
PIR	T50788.
RefSeq	NP_568239.1. NM_121117.1.
UniGene	At.6008.
3D structure databases
HSSP	HSSP built from PDB template 2GFO based on UniProtKB P40818.
ProteinModelPortal	Q9LEW0.
SMR	Q9LEW0. Positions 30-533.
ModBase	Search...
Protein family/group databases
MEROPS	C19.A11.
Proteomic databases
PaxDb	Q9LEW0.
PRIDE	Q9LEW0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G10790.1; AT5G10790.1; AT5G10790.
GeneID	830946.
KEGG	ath:AT5G10790.
Organism-specific databases
TAIR	At5g10790.
Phylogenomic databases
eggNOG	COG5533.
HOGENOM	HOG000007260.
InParanoid	Q9LEW0.
KO	K11366.
OMA	RTKTKEN.
PhylomeDB	Q9LEW0.
ProtClustDB	CLSN2689591.
Gene expression databases
Genevestigator	Q9LEW0.
Family and domain databases
Gene3D	3.30.40.10. 1 hit.
InterPro	IPR018200. Pept_C19ubi-hydrolase_C_CS. IPR001394. Peptidase_C19. IPR013083. Znf_RING/FYVE/PHD. IPR001607. Znf_UBP. [Graphical view]
Pfam	PF00443. UCH. 1 hit. PF02148. zf-UBP. 1 hit. [Graphical view]
PROSITE	PS00972. UCH_2_1. 1 hit. PS00973. UCH_2_2. False negative. PS50235. UCH_2_3. 1 hit. PS50271. ZF_UBP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UBP22_ARATH

Accession

Primary (citable) accession number: Q9LEW0
Secondary accession number(s): Q9FPS5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 1, 2000
Last modified:	May 1, 2013
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program