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Protein

Ubiquitin carboxyl-terminal hydrolase 22

Gene

UBP22

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei186NucleophilePROSITE-ProRule annotation1
Active sitei491Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri54 – 113UBP-typePROSITE-ProRule annotationAdd BLAST60

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciARA:AT5G10790-MONOMER.
ReactomeiR-ATH-5689880. Ub-specific processing proteases.

Protein family/group databases

MEROPSiC19.A11.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 22 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 22
Short name:
AtUBP22
Ubiquitin thioesterase 22
Ubiquitin-specific-processing protease 22
Gene namesi
Name:UBP22
Ordered Locus Names:At5g10790
ORF Names:T30N20_60
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G10790.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003130481 – 557Ubiquitin carboxyl-terminal hydrolase 22Add BLAST557

Proteomic databases

PaxDbiQ9LEW0.

Expressioni

Gene expression databases

GenevisibleiQ9LEW0. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT5G10790.1.

Structurei

3D structure databases

ProteinModelPortaliQ9LEW0.
SMRiQ9LEW0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini177 – 531USPAdd BLAST355

Sequence similaritiesi

Belongs to the peptidase C19 family.Curated
Contains 1 UBP-type zinc finger.PROSITE-ProRule annotation
Contains 1 USP domain.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri54 – 113UBP-typePROSITE-ProRule annotationAdd BLAST60

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG1867. Eukaryota.
ENOG410XQQ0. LUCA.
HOGENOMiHOG000007260.
InParanoidiQ9LEW0.
KOiK11366.
OMAiYLLFYMV.
OrthoDBiEOG093608U4.
PhylomeDBiQ9LEW0.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR001394. Peptidase_C19_UCH.
IPR018200. USP_CS.
IPR028889. USP_dom.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9LEW0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSARISFLKN PDPCNHLSDY KLRYGTDGYK SFNNLFRCFN DARIKIKLQG
60 70 80 90 100
IDIPRCSYCS VYQKRLYICL ICRSISCSSH ILLHTQLNKG HDIAIDVERS
110 120 130 140 150
ELYCCACIDQ VYDSEFDEVV VSKQLFGLGM SVKSGADVVA VRSNKKRRLD
160 170 180 190 200
SQLIIGSNFL VSPRDRREKW TFPLGLRGLN NLGSTCFMNA VLQALVHAPP
210 220 230 240 250
LRNFWLSGQH NRDLCPRRTM GLLCLPCDLD VIFSAMFSGD RTPYSPAHLL
260 270 280 290 300
YSWWQHSTNL ATYEQQDSHE FFISLLDRIH ENEGKSKCLY QDNEECQCIT
310 320 330 340 350
HKAFSGLLRS DVTCTTCGST STTYDPFIDI SLTLDSMNGF SPADCRKNRY
360 370 380 390 400
SGGPSVNAIM PTLSGCLDFF TRSEKLGPDQ KLNCQSCGEK RESSKQMSIR
410 420 430 440 450
RLPLLLCLHV KRFEHSLTRK TSRKIDSYLQ YPFRLNMSPY LSSSIIGKRF
460 470 480 490 500
GNRIFAFDGE GEYDSSSSSS PSAEFEIFAV VTHKGMLESG HYVTYLRLKG
510 520 530 540 550
LWYRCDDAWI NEVEEEVVRG CECYMLFYAQ ETVIQKAHKE LSYQVISMAD

AFPFADC
Length:557
Mass (Da):63,518
Last modified:October 1, 2000 - v1
Checksum:i3CA6C313AF0FF82C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti111V → A in AAG42760 (PubMed:11115897).Curated1
Sequence conflicti231V → I in AAG42760 (PubMed:11115897).Curated1
Sequence conflicti416S → F in AAG42760 (PubMed:11115897).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF302670 mRNA. Translation: AAG42760.1.
AL365234 Genomic DNA. Translation: CAB96834.1.
CP002688 Genomic DNA. Translation: AED91597.1.
PIRiT50788.
RefSeqiNP_568239.1. NM_121117.2.
UniGeneiAt.6008.

Genome annotation databases

EnsemblPlantsiAT5G10790.1; AT5G10790.1; AT5G10790.
GeneIDi830946.
GrameneiAT5G10790.1; AT5G10790.1; AT5G10790.
KEGGiath:AT5G10790.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF302670 mRNA. Translation: AAG42760.1.
AL365234 Genomic DNA. Translation: CAB96834.1.
CP002688 Genomic DNA. Translation: AED91597.1.
PIRiT50788.
RefSeqiNP_568239.1. NM_121117.2.
UniGeneiAt.6008.

3D structure databases

ProteinModelPortaliQ9LEW0.
SMRiQ9LEW0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT5G10790.1.

Protein family/group databases

MEROPSiC19.A11.

Proteomic databases

PaxDbiQ9LEW0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G10790.1; AT5G10790.1; AT5G10790.
GeneIDi830946.
GrameneiAT5G10790.1; AT5G10790.1; AT5G10790.
KEGGiath:AT5G10790.

Organism-specific databases

TAIRiAT5G10790.

Phylogenomic databases

eggNOGiKOG1867. Eukaryota.
ENOG410XQQ0. LUCA.
HOGENOMiHOG000007260.
InParanoidiQ9LEW0.
KOiK11366.
OMAiYLLFYMV.
OrthoDBiEOG093608U4.
PhylomeDBiQ9LEW0.

Enzyme and pathway databases

BioCyciARA:AT5G10790-MONOMER.
ReactomeiR-ATH-5689880. Ub-specific processing proteases.

Miscellaneous databases

PROiQ9LEW0.

Gene expression databases

GenevisibleiQ9LEW0. AT.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR001394. Peptidase_C19_UCH.
IPR018200. USP_CS.
IPR028889. USP_dom.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUBP22_ARATH
AccessioniPrimary (citable) accession number: Q9LEW0
Secondary accession number(s): Q9FPS5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 1, 2000
Last modified: November 30, 2016
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.