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Q9LEW0

- UBP22_ARATH

UniProt

Q9LEW0 - UBP22_ARATH

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Protein

Ubiquitin carboxyl-terminal hydrolase 22

Gene

UBP22

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei186 – 1861NucleophilePROSITE-ProRule annotation
Active sitei491 – 4911Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri54 – 11360UBP-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. cysteine-type peptidase activity Source: UniProtKB-KW
  2. ubiquitinyl hydrolase activity Source: InterPro
  3. zinc ion binding Source: InterPro

GO - Biological processi

  1. ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciARA:AT5G10790-MONOMER.

Protein family/group databases

MEROPSiC19.A11.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 22 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 22
Short name:
AtUBP22
Ubiquitin thioesterase 22
Ubiquitin-specific-processing protease 22
Gene namesi
Name:UBP22
Ordered Locus Names:At5g10790
ORF Names:T30N20_60
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 5

Organism-specific databases

TAIRiAT5G10790.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 557557Ubiquitin carboxyl-terminal hydrolase 22PRO_0000313048Add
BLAST

Proteomic databases

PaxDbiQ9LEW0.
PRIDEiQ9LEW0.

Expressioni

Gene expression databases

GenevestigatoriQ9LEW0.

Structurei

3D structure databases

ProteinModelPortaliQ9LEW0.
SMRiQ9LEW0. Positions 30-533.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini177 – 531355USPAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase C19 family.Curated
Contains 1 UBP-type zinc finger.PROSITE-ProRule annotation
Contains 1 USP domain.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri54 – 11360UBP-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiCOG5533.
HOGENOMiHOG000007260.
InParanoidiQ9LEW0.
KOiK11366.
OMAiVENWKQN.
PhylomeDBiQ9LEW0.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9LEW0 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSARISFLKN PDPCNHLSDY KLRYGTDGYK SFNNLFRCFN DARIKIKLQG
60 70 80 90 100
IDIPRCSYCS VYQKRLYICL ICRSISCSSH ILLHTQLNKG HDIAIDVERS
110 120 130 140 150
ELYCCACIDQ VYDSEFDEVV VSKQLFGLGM SVKSGADVVA VRSNKKRRLD
160 170 180 190 200
SQLIIGSNFL VSPRDRREKW TFPLGLRGLN NLGSTCFMNA VLQALVHAPP
210 220 230 240 250
LRNFWLSGQH NRDLCPRRTM GLLCLPCDLD VIFSAMFSGD RTPYSPAHLL
260 270 280 290 300
YSWWQHSTNL ATYEQQDSHE FFISLLDRIH ENEGKSKCLY QDNEECQCIT
310 320 330 340 350
HKAFSGLLRS DVTCTTCGST STTYDPFIDI SLTLDSMNGF SPADCRKNRY
360 370 380 390 400
SGGPSVNAIM PTLSGCLDFF TRSEKLGPDQ KLNCQSCGEK RESSKQMSIR
410 420 430 440 450
RLPLLLCLHV KRFEHSLTRK TSRKIDSYLQ YPFRLNMSPY LSSSIIGKRF
460 470 480 490 500
GNRIFAFDGE GEYDSSSSSS PSAEFEIFAV VTHKGMLESG HYVTYLRLKG
510 520 530 540 550
LWYRCDDAWI NEVEEEVVRG CECYMLFYAQ ETVIQKAHKE LSYQVISMAD

AFPFADC
Length:557
Mass (Da):63,518
Last modified:October 1, 2000 - v1
Checksum:i3CA6C313AF0FF82C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti111 – 1111V → A in AAG42760. (PubMed:11115897)Curated
Sequence conflicti231 – 2311V → I in AAG42760. (PubMed:11115897)Curated
Sequence conflicti416 – 4161S → F in AAG42760. (PubMed:11115897)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF302670 mRNA. Translation: AAG42760.1.
AL365234 Genomic DNA. Translation: CAB96834.1.
CP002688 Genomic DNA. Translation: AED91597.1.
PIRiT50788.
RefSeqiNP_568239.1. NM_121117.1.
UniGeneiAt.6008.

Genome annotation databases

EnsemblPlantsiAT5G10790.1; AT5G10790.1; AT5G10790.
GeneIDi830946.
KEGGiath:AT5G10790.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF302670 mRNA. Translation: AAG42760.1 .
AL365234 Genomic DNA. Translation: CAB96834.1 .
CP002688 Genomic DNA. Translation: AED91597.1 .
PIRi T50788.
RefSeqi NP_568239.1. NM_121117.1.
UniGenei At.6008.

3D structure databases

ProteinModelPortali Q9LEW0.
SMRi Q9LEW0. Positions 30-533.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi C19.A11.

Proteomic databases

PaxDbi Q9LEW0.
PRIDEi Q9LEW0.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT5G10790.1 ; AT5G10790.1 ; AT5G10790 .
GeneIDi 830946.
KEGGi ath:AT5G10790.

Organism-specific databases

TAIRi AT5G10790.

Phylogenomic databases

eggNOGi COG5533.
HOGENOMi HOG000007260.
InParanoidi Q9LEW0.
KOi K11366.
OMAi VENWKQN.
PhylomeDBi Q9LEW0.

Enzyme and pathway databases

BioCyci ARA:AT5G10790-MONOMER.

Miscellaneous databases

PROi Q9LEW0.

Gene expression databases

Genevestigatori Q9LEW0.

Family and domain databases

Gene3Di 3.30.40.10. 1 hit.
InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view ]
Pfami PF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view ]
PROSITEi PS00972. USP_1. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The ubiquitin-specific protease family from Arabidopsis. AtUBP1 and 2 are required for the resistance to the amino acid analog canavanine."
    Yan N., Doelling J.H., Falbel T.G., Durski A.M., Vierstra R.D.
    Plant Physiol. 124:1828-1843(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY ORGANIZATION, NOMENCLATURE.
    Strain: cv. Columbia.
  2. "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
    Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.
    , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
    Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.

Entry informationi

Entry nameiUBP22_ARATH
AccessioniPrimary (citable) accession number: Q9LEW0
Secondary accession number(s): Q9FPS5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 1, 2000
Last modified: October 1, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3