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Q9LEW0

- UBP22_ARATH

UniProt

Q9LEW0 - UBP22_ARATH

Protein

Ubiquitin carboxyl-terminal hydrolase 22

Gene

UBP22

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 83 (01 Oct 2014)
      Sequence version 1 (01 Oct 2000)
      Previous versions | rss
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    Functioni

    Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins By similarity.By similarity

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei186 – 1861NucleophilePROSITE-ProRule annotation
    Active sitei491 – 4911Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri54 – 11360UBP-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. cysteine-type peptidase activity Source: UniProtKB-KW
    2. ubiquitinyl hydrolase activity Source: InterPro
    3. zinc ion binding Source: InterPro

    GO - Biological processi

    1. ubiquitin-dependent protein catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Ubl conjugation pathway

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciARA:AT5G10790-MONOMER.

    Protein family/group databases

    MEROPSiC19.A11.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin carboxyl-terminal hydrolase 22 (EC:3.4.19.12)
    Alternative name(s):
    Deubiquitinating enzyme 22
    Short name:
    AtUBP22
    Ubiquitin thioesterase 22
    Ubiquitin-specific-processing protease 22
    Gene namesi
    Name:UBP22
    Ordered Locus Names:At5g10790
    ORF Names:T30N20_60
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G10790.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 557557Ubiquitin carboxyl-terminal hydrolase 22PRO_0000313048Add
    BLAST

    Proteomic databases

    PaxDbiQ9LEW0.
    PRIDEiQ9LEW0.

    Expressioni

    Gene expression databases

    GenevestigatoriQ9LEW0.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9LEW0.
    SMRiQ9LEW0. Positions 30-533.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini177 – 531355USPAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase C19 family.Curated
    Contains 1 UBP-type zinc finger.PROSITE-ProRule annotation
    Contains 1 USP domain.Curated

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri54 – 11360UBP-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiCOG5533.
    HOGENOMiHOG000007260.
    InParanoidiQ9LEW0.
    KOiK11366.
    OMAiVENWKQN.
    PhylomeDBiQ9LEW0.

    Family and domain databases

    Gene3Di3.30.40.10. 1 hit.
    InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    IPR013083. Znf_RING/FYVE/PHD.
    IPR001607. Znf_UBP.
    [Graphical view]
    PfamiPF00443. UCH. 1 hit.
    PF02148. zf-UBP. 1 hit.
    [Graphical view]
    PROSITEiPS00972. USP_1. 1 hit.
    PS50235. USP_3. 1 hit.
    PS50271. ZF_UBP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9LEW0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSARISFLKN PDPCNHLSDY KLRYGTDGYK SFNNLFRCFN DARIKIKLQG    50
    IDIPRCSYCS VYQKRLYICL ICRSISCSSH ILLHTQLNKG HDIAIDVERS 100
    ELYCCACIDQ VYDSEFDEVV VSKQLFGLGM SVKSGADVVA VRSNKKRRLD 150
    SQLIIGSNFL VSPRDRREKW TFPLGLRGLN NLGSTCFMNA VLQALVHAPP 200
    LRNFWLSGQH NRDLCPRRTM GLLCLPCDLD VIFSAMFSGD RTPYSPAHLL 250
    YSWWQHSTNL ATYEQQDSHE FFISLLDRIH ENEGKSKCLY QDNEECQCIT 300
    HKAFSGLLRS DVTCTTCGST STTYDPFIDI SLTLDSMNGF SPADCRKNRY 350
    SGGPSVNAIM PTLSGCLDFF TRSEKLGPDQ KLNCQSCGEK RESSKQMSIR 400
    RLPLLLCLHV KRFEHSLTRK TSRKIDSYLQ YPFRLNMSPY LSSSIIGKRF 450
    GNRIFAFDGE GEYDSSSSSS PSAEFEIFAV VTHKGMLESG HYVTYLRLKG 500
    LWYRCDDAWI NEVEEEVVRG CECYMLFYAQ ETVIQKAHKE LSYQVISMAD 550
    AFPFADC 557
    Length:557
    Mass (Da):63,518
    Last modified:October 1, 2000 - v1
    Checksum:i3CA6C313AF0FF82C
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti111 – 1111V → A in AAG42760. (PubMed:11115897)Curated
    Sequence conflicti231 – 2311V → I in AAG42760. (PubMed:11115897)Curated
    Sequence conflicti416 – 4161S → F in AAG42760. (PubMed:11115897)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF302670 mRNA. Translation: AAG42760.1.
    AL365234 Genomic DNA. Translation: CAB96834.1.
    CP002688 Genomic DNA. Translation: AED91597.1.
    PIRiT50788.
    RefSeqiNP_568239.1. NM_121117.1.
    UniGeneiAt.6008.

    Genome annotation databases

    EnsemblPlantsiAT5G10790.1; AT5G10790.1; AT5G10790.
    GeneIDi830946.
    KEGGiath:AT5G10790.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF302670 mRNA. Translation: AAG42760.1 .
    AL365234 Genomic DNA. Translation: CAB96834.1 .
    CP002688 Genomic DNA. Translation: AED91597.1 .
    PIRi T50788.
    RefSeqi NP_568239.1. NM_121117.1.
    UniGenei At.6008.

    3D structure databases

    ProteinModelPortali Q9LEW0.
    SMRi Q9LEW0. Positions 30-533.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi C19.A11.

    Proteomic databases

    PaxDbi Q9LEW0.
    PRIDEi Q9LEW0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G10790.1 ; AT5G10790.1 ; AT5G10790 .
    GeneIDi 830946.
    KEGGi ath:AT5G10790.

    Organism-specific databases

    TAIRi AT5G10790.

    Phylogenomic databases

    eggNOGi COG5533.
    HOGENOMi HOG000007260.
    InParanoidi Q9LEW0.
    KOi K11366.
    OMAi VENWKQN.
    PhylomeDBi Q9LEW0.

    Enzyme and pathway databases

    BioCyci ARA:AT5G10790-MONOMER.

    Miscellaneous databases

    PROi Q9LEW0.

    Gene expression databases

    Genevestigatori Q9LEW0.

    Family and domain databases

    Gene3Di 3.30.40.10. 1 hit.
    InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    IPR013083. Znf_RING/FYVE/PHD.
    IPR001607. Znf_UBP.
    [Graphical view ]
    Pfami PF00443. UCH. 1 hit.
    PF02148. zf-UBP. 1 hit.
    [Graphical view ]
    PROSITEi PS00972. USP_1. 1 hit.
    PS50235. USP_3. 1 hit.
    PS50271. ZF_UBP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The ubiquitin-specific protease family from Arabidopsis. AtUBP1 and 2 are required for the resistance to the amino acid analog canavanine."
      Yan N., Doelling J.H., Falbel T.G., Durski A.M., Vierstra R.D.
      Plant Physiol. 124:1828-1843(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY ORGANIZATION, NOMENCLATURE.
      Strain: cv. Columbia.
    2. "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
      Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.
      , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
      Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.

    Entry informationi

    Entry nameiUBP22_ARATH
    AccessioniPrimary (citable) accession number: Q9LEW0
    Secondary accession number(s): Q9FPS5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: October 1, 2000
    Last modified: October 1, 2014
    This is version 83 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3