Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q9LEW0 (UBP22_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin carboxyl-terminal hydrolase 22

EC=3.4.19.12
Alternative name(s):
Deubiquitinating enzyme 22
Short name=AtUBP22
Ubiquitin thioesterase 22
Ubiquitin-specific-processing protease 22
Gene names
Name:UBP22
Ordered Locus Names:At5g10790
ORF Names:T30N20_60
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length557 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins By similarity.

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sequence similarities

Belongs to the peptidase C19 family.

Contains 1 UBP-type zinc finger.

Contains 1 USP domain.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 557557Ubiquitin carboxyl-terminal hydrolase 22
PRO_0000313048

Regions

Domain177 – 531355USP
Zinc finger54 – 11360UBP-type

Sites

Active site1861Nucleophile By similarity
Active site4911Proton acceptor By similarity

Experimental info

Sequence conflict1111V → A in AAG42760. Ref.1
Sequence conflict2311V → I in AAG42760. Ref.1
Sequence conflict4161S → F in AAG42760. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q9LEW0 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 3CA6C313AF0FF82C

FASTA55763,518
        10         20         30         40         50         60 
MSARISFLKN PDPCNHLSDY KLRYGTDGYK SFNNLFRCFN DARIKIKLQG IDIPRCSYCS 

        70         80         90        100        110        120 
VYQKRLYICL ICRSISCSSH ILLHTQLNKG HDIAIDVERS ELYCCACIDQ VYDSEFDEVV 

       130        140        150        160        170        180 
VSKQLFGLGM SVKSGADVVA VRSNKKRRLD SQLIIGSNFL VSPRDRREKW TFPLGLRGLN 

       190        200        210        220        230        240 
NLGSTCFMNA VLQALVHAPP LRNFWLSGQH NRDLCPRRTM GLLCLPCDLD VIFSAMFSGD 

       250        260        270        280        290        300 
RTPYSPAHLL YSWWQHSTNL ATYEQQDSHE FFISLLDRIH ENEGKSKCLY QDNEECQCIT 

       310        320        330        340        350        360 
HKAFSGLLRS DVTCTTCGST STTYDPFIDI SLTLDSMNGF SPADCRKNRY SGGPSVNAIM 

       370        380        390        400        410        420 
PTLSGCLDFF TRSEKLGPDQ KLNCQSCGEK RESSKQMSIR RLPLLLCLHV KRFEHSLTRK 

       430        440        450        460        470        480 
TSRKIDSYLQ YPFRLNMSPY LSSSIIGKRF GNRIFAFDGE GEYDSSSSSS PSAEFEIFAV 

       490        500        510        520        530        540 
VTHKGMLESG HYVTYLRLKG LWYRCDDAWI NEVEEEVVRG CECYMLFYAQ ETVIQKAHKE 

       550 
LSYQVISMAD AFPFADC 

« Hide

References

« Hide 'large scale' references
[1]"The ubiquitin-specific protease family from Arabidopsis. AtUBP1 and 2 are required for the resistance to the amino acid analog canavanine."
Yan N., Doelling J.H., Falbel T.G., Durski A.M., Vierstra R.D.
Plant Physiol. 124:1828-1843(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY ORGANIZATION, NOMENCLATURE.
Strain: cv. Columbia.
[2]"Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K. expand/collapse author list , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF302670 mRNA. Translation: AAG42760.1.
AL365234 Genomic DNA. Translation: CAB96834.1.
CP002688 Genomic DNA. Translation: AED91597.1.
PIRT50788.
RefSeqNP_568239.1. NM_121117.1.
UniGeneAt.6008.

3D structure databases

ProteinModelPortalQ9LEW0.
SMRQ9LEW0. Positions 30-533.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSC19.A11.

Proteomic databases

PaxDbQ9LEW0.
PRIDEQ9LEW0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G10790.1; AT5G10790.1; AT5G10790.
GeneID830946.
KEGGath:AT5G10790.

Organism-specific databases

TAIRAT5G10790.

Phylogenomic databases

eggNOGCOG5533.
HOGENOMHOG000007260.
InParanoidQ9LEW0.
KOK11366.
OMAVENWKQN.
PhylomeDBQ9LEW0.

Enzyme and pathway databases

BioCycARA:AT5G10790-MONOMER.

Gene expression databases

GenevestigatorQ9LEW0.

Family and domain databases

Gene3D3.30.40.10. 1 hit.
InterProIPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view]
PfamPF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view]
PROSITEPS00972. USP_1. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROQ9LEW0.

Entry information

Entry nameUBP22_ARATH
AccessionPrimary (citable) accession number: Q9LEW0
Secondary accession number(s): Q9FPS5
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 1, 2000
Last modified: May 14, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names