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Protein

CDPK-related kinase 7

Gene

CRK7

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in signal transduction pathways that involve calcium as a second messenger.By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by calcium and calmodulin. Autophosphorylation may play an important role in the regulation of the kinase activity (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei156 – 1561ATPPROSITE-ProRule annotation
Active sitei252 – 2521Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi130 – 1389ATPPROSITE-ProRule annotation
Calcium bindingi441 – 452121By similarityAdd
BLAST
Calcium bindingi478 – 489122By similarityAdd
BLAST
Calcium bindingi518 – 529123By similarityAdd
BLAST
Calcium bindingi550 – 561124By similarityAdd
BLAST

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Calcium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT3G56760-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
CDPK-related kinase 7 (EC:2.7.11.1)
Short name:
AtCRK7
Alternative name(s):
Calcium/calmodulin-dependent protein kinase CRK7
Gene namesi
Name:CRK7
Ordered Locus Names:At3g56760
ORF Names:T8M16.90
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G56760.

Subcellular locationi

GO - Cellular componenti

  • membrane Source: TAIR
  • plasma membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 577576CDPK-related kinase 7PRO_0000420534Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycineBy similarity
Modified residuei334 – 3341Phosphoserine; by CPK1, CPK10 and CPK341 Publication

Post-translational modificationi

Autophosphorylated.By similarity

Keywords - PTMi

Lipoprotein, Myristate, Phosphoprotein

Proteomic databases

PaxDbiQ9LET1.
PRIDEiQ9LET1.

PTM databases

iPTMnetiQ9LET1.

Expressioni

Gene expression databases

GenevisibleiQ9LET1. AT.

Interactioni

Subunit structurei

Binds calmodulin (CaM) in a calcium-dependent manner.By similarity

Protein-protein interaction databases

STRINGi3702.AT3G56760.1.

Structurei

3D structure databases

ProteinModelPortaliQ9LET1.
SMRiQ9LET1. Positions 120-566.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini124 – 386263Protein kinasePROSITE-ProRule annotationAdd
BLAST
Domaini428 – 46437EF-hand 1Add
BLAST
Domaini465 – 50036EF-hand 2Add
BLAST
Domaini501 – 54040EF-hand 3Add
BLAST
Domaini543 – 57230EF-hand 4Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni391 – 42131Autoinhibitory domainBy similarityAdd
BLAST
Regioni410 – 43021Calmodulin binding (CaMBD)By similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi29 – 6335Ser-richAdd
BLAST

Domaini

There are 3 contiguous domains conserved in the CDPK subfamily: a kinase domain, an autoinhibitory (junction) domain and a calmodulin-like domain. The autoinhibitory domain (391-421) inactivates kinase activity under calcium-free conditions (By similarity).By similarity

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.PROSITE-ProRule annotation
Contains 4 EF-hand domains.Curated
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0032. Eukaryota.
ENOG410XRMJ. LUCA.
HOGENOMiHOG000233030.
KOiK00924.
OMAiTYLQEQF.
PhylomeDBiQ9LET1.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9LET1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGLCHGKPIE QQSKNLPISN EIEETPKNSS QKAKSSGFPF YSPSPLPSLF
60 70 80 90 100
KTSPAVSSSS VSSTPLRIFK RPFPPPSPAK HIRALLARRH GSVKPNEASI
110 120 130 140 150
PEGSECEVGL DKKFGFSKQF ASHYEIDGEV GRGHFGYTCS AKGKKGSLKG
160 170 180 190 200
QDVAVKVIPK SKMTTAIAIE DVRREVKILR ALTGHKNLVQ FYDAFEDDEN
210 220 230 240 250
VYIVMELCQG GELLDKILQR GGKYSEVDAK KVMIQILSVV AYCHLQGVVH
260 270 280 290 300
RDLKPENFLF TTKDESSPLK AIDFGLSDYV RPDERLNDIV GSAYYVAPEV
310 320 330 340 350
LHRTYGTEAD MWSIGVIAYI LLCGSRPFWA RSESGIFRAV LKAEPNFEEA
360 370 380 390 400
PWPSLSPDAV DFVKRLLNKD YRKRLTAAQA LCHPWLVGSH ELKIPSDMII
410 420 430 440 450
YKLVKVYIMS SSLRKSALAA LAKTLTVPQL TYLQEQFNLL GPSKNGYISM
460 470 480 490 500
QNYKTAILKS STEATKDSRV LDFVHMISCL QYKKLDFEEF CASALSVYQL
510 520 530 540 550
EAMETWEQHA RRAYELYEKD GNRVIMIEEL ATELGLGPSV PVHVVLQDWI
560 570
RHSDGKLSFL GFVRLLHGVS SRTLQKA
Length:577
Mass (Da):64,547
Last modified:October 1, 2000 - v1
Checksum:i7E1EAA8E48E8C934
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL390921 Genomic DNA. Translation: CAC00739.1.
CP002686 Genomic DNA. Translation: AEE79562.1.
PIRiT51264.
RefSeqiNP_191235.1. NM_115535.2.
UniGeneiAt.34902.

Genome annotation databases

EnsemblPlantsiAT3G56760.1; AT3G56760.1; AT3G56760.
GeneIDi824843.
GrameneiAT3G56760.1; AT3G56760.1; AT3G56760.
KEGGiath:AT3G56760.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL390921 Genomic DNA. Translation: CAC00739.1.
CP002686 Genomic DNA. Translation: AEE79562.1.
PIRiT51264.
RefSeqiNP_191235.1. NM_115535.2.
UniGeneiAt.34902.

3D structure databases

ProteinModelPortaliQ9LET1.
SMRiQ9LET1. Positions 120-566.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT3G56760.1.

PTM databases

iPTMnetiQ9LET1.

Proteomic databases

PaxDbiQ9LET1.
PRIDEiQ9LET1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G56760.1; AT3G56760.1; AT3G56760.
GeneIDi824843.
GrameneiAT3G56760.1; AT3G56760.1; AT3G56760.
KEGGiath:AT3G56760.

Organism-specific databases

TAIRiAT3G56760.

Phylogenomic databases

eggNOGiKOG0032. Eukaryota.
ENOG410XRMJ. LUCA.
HOGENOMiHOG000233030.
KOiK00924.
OMAiTYLQEQF.
PhylomeDBiQ9LET1.

Enzyme and pathway databases

BioCyciARA:AT3G56760-MONOMER.

Miscellaneous databases

PROiQ9LET1.

Gene expression databases

GenevisibleiQ9LET1. AT.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
    Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
    , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Calcium-regulated protein kinases of plants."
    Harmon A.C.
    Gravit. Space Biol. Bull. 16:83-90(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW, GENE FAMILY.
  4. Cited for: GENE FAMILY, NOMENCLATURE.
    Strain: cv. Columbia.
  5. "Biochemical and expression analysis of an Arabidopsis calcium-dependent protein kinase-related kinase."
    Du W., Wang Y., Liang S., Lu Y.-T.
    Plant Sci. 168:1181-1192(2005)
    [AGRICOLA] [Europe PMC]
    Cited for: GENE FAMILY.
    Strain: cv. Columbia.
  6. "Calcium-dependent protein kinases from Arabidopsis show substrate specificity differences in an analysis of 103 substrates."
    Curran A., Chang I.-F., Chang C.-L., Garg S., Miguel R.M., Barron Y.D., Li Y., Romanowsky S., Cushman J.C., Gribskov M., Harmon A.C., Harper J.F.
    Front. Plant Sci. 2:36-36(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION AT SER-334.

Entry informationi

Entry nameiCAMK7_ARATH
AccessioniPrimary (citable) accession number: Q9LET1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 9, 2013
Last sequence update: October 1, 2000
Last modified: May 11, 2016
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.