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Protein

CDPK-related kinase 7

Gene

CRK7

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in signal transduction pathways that involve calcium as a second messenger.By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by calcium and calmodulin. Autophosphorylation may play an important role in the regulation of the kinase activity (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei156ATPPROSITE-ProRule annotation1
Active sitei252Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi130 – 138ATPPROSITE-ProRule annotation9
Calcium bindingi441 – 4521By similarityAdd BLAST12
Calcium bindingi478 – 4892By similarityAdd BLAST12
Calcium bindingi518 – 5293By similarityAdd BLAST12
Calcium bindingi550 – 5614By similarityAdd BLAST12

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Calcium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-ATH-3371571. HSF1-dependent transactivation.
R-ATH-4086398. Ca2+ pathway.
R-ATH-442729. CREB phosphorylation through the activation of CaMKII.
R-ATH-5578775. Ion homeostasis.
R-ATH-936837. Ion transport by P-type ATPases.

Names & Taxonomyi

Protein namesi
Recommended name:
CDPK-related kinase 7 (EC:2.7.11.1)
Short name:
AtCRK7
Alternative name(s):
Calcium/calmodulin-dependent protein kinase CRK7
Gene namesi
Name:CRK7
Ordered Locus Names:At3g56760
ORF Names:T8M16.90
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G56760.
TAIRilocus:2103655. AT3G56760.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00004205342 – 577CDPK-related kinase 7Add BLAST576

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycineBy similarity1
Modified residuei292PhosphoserineBy similarity1
Modified residuei334Phosphoserine; by CPK1, CPK10 and CPK341 Publication1
Modified residuei558PhosphoserineBy similarity1

Post-translational modificationi

Autophosphorylated.By similarity

Keywords - PTMi

Lipoprotein, Myristate, Phosphoprotein

Proteomic databases

PaxDbiQ9LET1.
PRIDEiQ9LET1.

PTM databases

iPTMnetiQ9LET1.

Expressioni

Gene expression databases

GenevisibleiQ9LET1. AT.

Interactioni

Subunit structurei

Binds calmodulin (CaM) in a calcium-dependent manner.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi3702.AT3G56760.1.

Structurei

3D structure databases

ProteinModelPortaliQ9LET1.
SMRiQ9LET1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini124 – 386Protein kinasePROSITE-ProRule annotationAdd BLAST263
Domaini428 – 464EF-hand 1Add BLAST37
Domaini465 – 500EF-hand 2Add BLAST36
Domaini501 – 540EF-hand 3Add BLAST40
Domaini543 – 572EF-hand 4Add BLAST30

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni391 – 421Autoinhibitory domainBy similarityAdd BLAST31
Regioni410 – 430Calmodulin binding (CaMBD)By similarityAdd BLAST21

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi29 – 63Ser-richAdd BLAST35

Domaini

There are 3 contiguous domains conserved in the CDPK subfamily: a kinase domain, an autoinhibitory (junction) domain and a calmodulin-like domain. The autoinhibitory domain (391-421) inactivates kinase activity under calcium-free conditions (By similarity).By similarity

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0032. Eukaryota.
ENOG410XRMJ. LUCA.
HOGENOMiHOG000233030.
KOiK00924.
OMAiYKSSPAN.
OrthoDBiEOG093606GB.
PhylomeDBiQ9LET1.

Family and domain databases

InterProiView protein in InterPro
IPR011992. EF-hand-dom_pair.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
PfamiView protein in Pfam
PF00069. Pkinase. 1 hit.
SMARTiView protein in SMART
SM00220. S_TKc. 1 hit.
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiView protein in PROSITE
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9LET1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGLCHGKPIE QQSKNLPISN EIEETPKNSS QKAKSSGFPF YSPSPLPSLF
60 70 80 90 100
KTSPAVSSSS VSSTPLRIFK RPFPPPSPAK HIRALLARRH GSVKPNEASI
110 120 130 140 150
PEGSECEVGL DKKFGFSKQF ASHYEIDGEV GRGHFGYTCS AKGKKGSLKG
160 170 180 190 200
QDVAVKVIPK SKMTTAIAIE DVRREVKILR ALTGHKNLVQ FYDAFEDDEN
210 220 230 240 250
VYIVMELCQG GELLDKILQR GGKYSEVDAK KVMIQILSVV AYCHLQGVVH
260 270 280 290 300
RDLKPENFLF TTKDESSPLK AIDFGLSDYV RPDERLNDIV GSAYYVAPEV
310 320 330 340 350
LHRTYGTEAD MWSIGVIAYI LLCGSRPFWA RSESGIFRAV LKAEPNFEEA
360 370 380 390 400
PWPSLSPDAV DFVKRLLNKD YRKRLTAAQA LCHPWLVGSH ELKIPSDMII
410 420 430 440 450
YKLVKVYIMS SSLRKSALAA LAKTLTVPQL TYLQEQFNLL GPSKNGYISM
460 470 480 490 500
QNYKTAILKS STEATKDSRV LDFVHMISCL QYKKLDFEEF CASALSVYQL
510 520 530 540 550
EAMETWEQHA RRAYELYEKD GNRVIMIEEL ATELGLGPSV PVHVVLQDWI
560 570
RHSDGKLSFL GFVRLLHGVS SRTLQKA
Length:577
Mass (Da):64,547
Last modified:October 1, 2000 - v1
Checksum:i7E1EAA8E48E8C934
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL390921 Genomic DNA. Translation: CAC00739.1.
CP002686 Genomic DNA. Translation: AEE79562.1.
PIRiT51264.
RefSeqiNP_191235.1. NM_115535.3.
UniGeneiAt.34902.

Genome annotation databases

EnsemblPlantsiAT3G56760.1; AT3G56760.1; AT3G56760.
GeneIDi824843.
GrameneiAT3G56760.1; AT3G56760.1; AT3G56760.
KEGGiath:AT3G56760.

Similar proteinsi

Entry informationi

Entry nameiCAMK7_ARATH
AccessioniPrimary (citable) accession number: Q9LET1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 9, 2013
Last sequence update: October 1, 2000
Last modified: September 27, 2017
This is version 139 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families