Reviewed, UniProtKB/Swiss-Prot Q9LEK8 (PIN1_DIGLA)

Names and origin

Protein names

Recommended name:
    Peptidyl-prolyl cis-trans isomerase 1
    EC=5.2.1.8
Alternative name(s):
    Rotamase Pin1
    PPIase Pin1
    DlPar13

Gene names

Name:

PARV12.8

Organism

Digitalis lanata (Foxglove)

Taxonomic identifier

49450 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Lamiales › Plantaginaceae › Digitalideae › Digitalis

Protein attributes

Sequence length	118 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Prolyl cis/trans isomerase with specificity for phospho-Ser-Pro bonds. Ref.1
Catalytic activity	Peptidylproline (omega=180) = peptidylproline (omega=0).
Enzyme regulation	Inhibited in vitro by juglone.
Subcellular location	Cytoplasm. Nucleus Ref.1.
Tissue specificity	Expressed in roots, stems, leaves, flowers and seedlings. Ref.1
Post-translational modification	The N-terminus is blocked.
Miscellaneous	Like all plant Pin1-type PPIases, do not contain the N-terminal WW domain found in other eukaryotic parvulins, but contains a four-amino acid insertion next to the phospho-specific recognition site of the active site. These extra amino acids may be important for mediating the substrate interaction of plant enzymes. Three amino acid residues (P79S, E91D and A95G) differ when compared with the partial sequence of purified protein. The existence of two or more genes coding for this protein is not exluded.
Sequence similarities	Belongs to the ppiC/parvulin rotamase family. Contains 1 PpiC domain.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Molecular function	Isomerase Rotamase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	protein folding Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	peptidyl-prolyl cis-trans isomerase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 118	118	Peptidyl-prolyl cis-trans isomerase 1		PRO_0000193441
Regions
	Domain	3 – 118	116	PpiC

Sequences

	Sequence		Length	Mass (Da)
	Q9LEK8-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 131B74FB4AC3F229	FASTA	118	12,834
	10 20 30 40 50 60 MSSEKVRASH ILIKHQGSRR KSSWKDPDGS LISATTRDDA VSQLQSLRQE LLSDPASFSD 70 80 90 100 110 LASRHSHCSS AKRGGDLGPF GRGQMQKPFE EATFALKVGE ISDIVDTDSG VHIIKRTG

References

[1]

"Functional replacement of the essential ess1 in yeast by the plant parvulin DlPar13." Metzner M., Stoller G., Ruecknagel P., Lu K.P., Fischer G., Luckner M., Kuellertz G. J. Biol. Chem. 276:13524-13529(2001) [PubMed: 11118437] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, FUNCTION. Strain: cv. VIII.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ133755 mRNA. Translation: CAB94994.1.
3D structure databases
SMR	Q9LEK8. Positions 2-118.
ModBase	Search...
Enzyme and pathway databases
BRENDA	5.2.1.8. 255268.
Family and domain databases
InterPro	IPR000297. PPIase_PpiC. [Graphical view]
Pfam	PF00639. Rotamase. 1 hit. [Graphical view]
PROSITE	PS01096. PPIC_PPIASE_1. 1 hit. PS50198. PPIC_PPIASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PIN1_DIGLA

Accession

Primary (citable) accession number: Q9LEK8

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2003
Last sequence update:	October 1, 2000
Last modified:	January 19, 2010
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)