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Protein

Enolase 1

Gene

ENO1

Organism
Hevea brasiliensis (Para rubber tree) (Siphonia brasiliensis)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

2-phospho-D-glycerate = phosphoenolpyruvate + H2O.

Cofactori

Mg2+By similarityNote: Mg2+ is required for catalysis and for stabilizing the dimer.By similarity

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. no protein annotated in this organism
  3. no protein annotated in this organism
  4. Enolase 1 (ENO1), Enolase 2 (ENO2)
  5. no protein annotated in this organism
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei164SubstrateBy similarity1
Binding sitei173SubstrateBy similarity1
Active sitei216Proton donorBy similarity1
Metal bindingi251MagnesiumBy similarity1
Metal bindingi301MagnesiumBy similarity1
Binding sitei301SubstrateBy similarity1
Metal bindingi328MagnesiumBy similarity1
Binding sitei328SubstrateBy similarity1
Active sitei353Proton acceptorBy similarity1
Binding sitei404SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-12858.
BRENDAi4.2.1.11. 2665.
UniPathwayiUPA00109; UER00187.

Names & Taxonomyi

Protein namesi
Recommended name:
Enolase 1 (EC:4.2.1.11)
Alternative name(s):
2-phospho-D-glycerate hydro-lyase 1
2-phosphoglycerate dehydratase 1
Allergen: Hev b 9
Gene namesi
Name:ENO1
OrganismiHevea brasiliensis (Para rubber tree) (Siphonia brasiliensis)
Taxonomic identifieri3981 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsMalpighialesEuphorbiaceaeCrotonoideaeMicrandreaeHevea

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Allergenic propertiesi

Causes an allergic reaction in human. Involved in latex allergic reactions.1 Publication

Keywords - Diseasei

Allergen

Protein family/group databases

Allergomei3317. Hev b 9.0101.
404. Hev b 9.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001340691 – 445Enolase 1Add BLAST445

Proteomic databases

PRIDEiQ9LEJ0.

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ9LEJ0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni380 – 383Substrate bindingBy similarity4

Sequence similaritiesi

Belongs to the enolase family.Curated

Family and domain databases

CDDicd03313. enolase. 1 hit.
Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
HAMAPiMF_00318. Enolase. 1 hit.
InterProiIPR000941. Enolase.
IPR020810. Enolase_C.
IPR029065. Enolase_C-like.
IPR020809. Enolase_CS.
IPR020811. Enolase_N.
IPR029017. Enolase_N-like.
[Graphical view]
PANTHERiPTHR11902. PTHR11902. 1 hit.
PfamiPF00113. Enolase_C. 1 hit.
PF03952. Enolase_N. 1 hit.
[Graphical view]
PIRSFiPIRSF001400. Enolase. 1 hit.
PRINTSiPR00148. ENOLASE.
SMARTiSM01192. Enolase_C. 1 hit.
SM01193. Enolase_N. 1 hit.
[Graphical view]
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
TIGRFAMsiTIGR01060. eno. 1 hit.
PROSITEiPS00164. ENOLASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9LEJ0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAITIVSVRA RQIFDSRGNP TVEADVKLSD GYLARAAVPS GASTGIYEAL
60 70 80 90 100
ELRDGGSDYL GKGVSKAVEN VNIIIGPALV GKDPTDQVGI DNFMVQQLDG
110 120 130 140 150
TVNEWGWCKQ KLGANAILAV SLAVCKAGAH VKGIPLYEHI ANLAGNKNLV
160 170 180 190 200
LPVPAFNVIN GGSHAGNKLA MQEFMILPVG ASSFKEAMKM GAEVYHHLKS
210 220 230 240 250
VIKKKYGQDA TNVGDEGGFA PNIQENKEGL ELLKTAIAKA GYTGKVVIGM
260 270 280 290 300
DVAASEFYGS DQTYDLNFKE ENNNGSQKIS GEALKDLYKS FVAEYPIVSI
310 320 330 340 350
EDPFDQDDWA HYAKLTSEIG EKVQIVGDDL LVTNPKRVEK AIKEKACNAL
360 370 380 390 400
LLKVNQIGSV TESIEAVKMS KRAGWGVMAS HRSGETEDTF IADLSVGLAT
410 420 430 440
GQIKTGAPCR SERLAKYNQL LRIEEELGSE AVYAGANFRK PVEPY
Length:445
Mass (Da):47,830
Last modified:October 1, 2000 - v1
Checksum:i238883FB249F5544
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ132580 mRNA. Translation: CAC00532.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ132580 mRNA. Translation: CAC00532.1.

3D structure databases

ProteinModelPortaliQ9LEJ0.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

Allergomei3317. Hev b 9.0101.
404. Hev b 9.

Proteomic databases

PRIDEiQ9LEJ0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00109; UER00187.
BioCyciMetaCyc:MONOMER-12858.
BRENDAi4.2.1.11. 2665.

Family and domain databases

CDDicd03313. enolase. 1 hit.
Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
HAMAPiMF_00318. Enolase. 1 hit.
InterProiIPR000941. Enolase.
IPR020810. Enolase_C.
IPR029065. Enolase_C-like.
IPR020809. Enolase_CS.
IPR020811. Enolase_N.
IPR029017. Enolase_N-like.
[Graphical view]
PANTHERiPTHR11902. PTHR11902. 1 hit.
PfamiPF00113. Enolase_C. 1 hit.
PF03952. Enolase_N. 1 hit.
[Graphical view]
PIRSFiPIRSF001400. Enolase. 1 hit.
PRINTSiPR00148. ENOLASE.
SMARTiSM01192. Enolase_C. 1 hit.
SM01193. Enolase_N. 1 hit.
[Graphical view]
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
TIGRFAMsiTIGR01060. eno. 1 hit.
PROSITEiPS00164. ENOLASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiENO1_HEVBR
AccessioniPrimary (citable) accession number: Q9LEJ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: October 1, 2000
Last modified: October 5, 2016
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. Allergens
    Nomenclature of allergens and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.