Reviewed, UniProtKB/Swiss-Prot Q9LEC8 (DHEB_NICPL)

Names and origin

Protein names

Recommended name:
    Glutamate dehydrogenase B
      Short name=GDH B
    EC=1.4.1.3

Gene names

Name:

GDHB

Organism

Nicotiana plumbaginifolia (Leadwort-leaved tobacco)

Taxonomic identifier

4092 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Nicotianoideae › Nicotianeae › Nicotiana

Protein attributes

Sequence length	411 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	L-glutamate + H2O + NAD(P)+ = 2-oxoglutarate + NH3 + NAD(P)H.
Sequence similarities	Belongs to the Glu/Leu/Phe/Val dehydrogenases family.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	amino acid metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	binding Inferred from electronic annotation. Source: InterPro glutamate dehydrogenase [NAD(P)+] activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 411	411	Glutamate dehydrogenase B		PRO_0000182751
Sites
	Active site	102	1	By similarity
Experimental info
	Sequence conflict	92	1	A → R in CAA69601. Ref.1
	Sequence conflict	368	1	A → D in CAA69601. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9LEC8-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 236D4915B8B670AE	FASTA	411	44,508
	10 20 30 40 50 60 MNALAATNRN FKLASRLLGL DSKLEQCLLI PFREIKVECT IPKDDGSLAT FIGFRVQHDN 70 80 90 100 110 120 ARGPMKGGIR YHPEVDPDEV NALAQLMTWK TAVANIPYGG AKGGIGCSPS DLSISELERL 130 140 150 160 170 180 TRVFTQKIHD LIGVHTDVPA PDMGTNPQTM AWILDEYSKF HGYSPAVVTG KPIDLGGSLG 190 200 210 220 230 240 RDAATGRGVL FAAEALLRDH GKSIAGQRFV VQGFGNVGSW AAQLITEQGG KIVAVSDITG 250 260 270 280 290 300 AIKNKNGIDI ASLLKHVKEN RGVKGFHGAD SIDPNSILVE DCDVLIPAAL GGVINRDNAK 310 320 330 340 350 360 DIKAKFIVEA ANHPTDPEAD EILAKKGVVI LPDIYANSGG VTVSYFEWVQ NIQGFMWDEE 370 380 390 400 410 RVNTELKAYM NRGFKDVKDM CKTHNCDLRM GAFTLGVNRV ARATTLRGWE A

References

[1]	"Isolation and characterization of two cDNA clones encoding for glutamate dehydrogenase in Nicotiana plumbaginifolia." Ficarelli A., Tassi F., Restivo F.M. Plant Cell Physiol. 40:339-342(1999) [PubMed: 10353219] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Leaf.
[2]	"Genomic organisation of GDH genes in Nicotiana plumbaginifolia." Restivo F.M., Panizzi N., De Monte G., Ficarelli A. Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Leaf.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Y08293 Genomic DNA. Translation: CAA69601.2. AJ277950 Genomic DNA. Translation: CAB94837.1.
PIR	T16982.
3D structure databases
HSSP	HSSP built from PDB template 1B26 based on UniProtKB P96110.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.4.1.3. 257040.
Family and domain databases
InterPro	IPR006095. Glu/Leu/Phe/Val_DH. IPR006096. Glu/Leu/Phe/Val_DH_C. IPR006097. Glu/Leu/Phe/Val_DH_dimer. IPR014362. Glu_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR11606:SF2. GLFV_DH. 1 hit.
Pfam	PF00208. ELFV_dehydrog. 1 hit. PF02812. ELFV_dehydrog_N. 1 hit. [Graphical view]
PIRSF	PIRSF000185. Glu_DH. 1 hit.
PRINTS	PR00082. GLFDHDRGNASE.
PROSITE	PS00074. GLFV_DEHYDROGENASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHEB_NICPL

Accession

Primary (citable) accession number: Q9LEC8
Secondary accession number(s): O24119

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	October 1, 2000
Last modified:	June 16, 2009
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)