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Protein

Glutamate dehydrogenase B

Gene

GDHB

Organism
Nicotiana plumbaginifolia (Leadwort-leaved tobacco) (Tex-Mex tobacco)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

L-glutamate + H2O + NAD(P)+ = 2-oxoglutarate + NH3 + NAD(P)H.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei102 – 1021PROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate dehydrogenase B (EC:1.4.1.3)
Short name:
GDH B
Gene namesi
Name:GDHB
OrganismiNicotiana plumbaginifolia (Leadwort-leaved tobacco) (Tex-Mex tobacco)
Taxonomic identifieri4092 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeNicotianoideaeNicotianeaeNicotiana

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 411411Glutamate dehydrogenase BPRO_0000182751Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ9LEC8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR006095. Glu/Leu/Phe/Val_DH.
IPR006096. Glu/Leu/Phe/Val_DH_C.
IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
IPR014362. Glu_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00208. ELFV_dehydrog. 1 hit.
PF02812. ELFV_dehydrog_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000185. Glu_DH. 1 hit.
PRINTSiPR00082. GLFDHDRGNASE.
SMARTiSM00839. ELFV_dehydrog. 1 hit.
[Graphical view]
PROSITEiPS00074. GLFV_DEHYDROGENASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9LEC8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNALAATNRN FKLASRLLGL DSKLEQCLLI PFREIKVECT IPKDDGSLAT
60 70 80 90 100
FIGFRVQHDN ARGPMKGGIR YHPEVDPDEV NALAQLMTWK TAVANIPYGG
110 120 130 140 150
AKGGIGCSPS DLSISELERL TRVFTQKIHD LIGVHTDVPA PDMGTNPQTM
160 170 180 190 200
AWILDEYSKF HGYSPAVVTG KPIDLGGSLG RDAATGRGVL FAAEALLRDH
210 220 230 240 250
GKSIAGQRFV VQGFGNVGSW AAQLITEQGG KIVAVSDITG AIKNKNGIDI
260 270 280 290 300
ASLLKHVKEN RGVKGFHGAD SIDPNSILVE DCDVLIPAAL GGVINRDNAK
310 320 330 340 350
DIKAKFIVEA ANHPTDPEAD EILAKKGVVI LPDIYANSGG VTVSYFEWVQ
360 370 380 390 400
NIQGFMWDEE RVNTELKAYM NRGFKDVKDM CKTHNCDLRM GAFTLGVNRV
410
ARATTLRGWE A
Length:411
Mass (Da):44,508
Last modified:October 1, 2000 - v1
Checksum:i236D4915B8B670AE
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti92 – 921A → R in CAA69601 (PubMed:10353219).Curated
Sequence conflicti368 – 3681A → D in CAA69601 (PubMed:10353219).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y08293 Genomic DNA. Translation: CAA69601.2.
AJ277950 Genomic DNA. Translation: CAB94837.1.
PIRiT16982.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y08293 Genomic DNA. Translation: CAA69601.2.
AJ277950 Genomic DNA. Translation: CAB94837.1.
PIRiT16982.

3D structure databases

ProteinModelPortaliQ9LEC8.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR006095. Glu/Leu/Phe/Val_DH.
IPR006096. Glu/Leu/Phe/Val_DH_C.
IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
IPR014362. Glu_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00208. ELFV_dehydrog. 1 hit.
PF02812. ELFV_dehydrog_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000185. Glu_DH. 1 hit.
PRINTSiPR00082. GLFDHDRGNASE.
SMARTiSM00839. ELFV_dehydrog. 1 hit.
[Graphical view]
PROSITEiPS00074. GLFV_DEHYDROGENASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Isolation and characterization of two cDNA clones encoding for glutamate dehydrogenase in Nicotiana plumbaginifolia."
    Ficarelli A., Tassi F., Restivo F.M.
    Plant Cell Physiol. 40:339-342(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Leaf.
  2. "Genomic organisation of GDH genes in Nicotiana plumbaginifolia."
    Restivo F.M., Panizzi N., De Monte G., Ficarelli A.
    Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Tissue: Leaf.

Entry informationi

Entry nameiDHEB_NICPL
AccessioniPrimary (citable) accession number: Q9LEC8
Secondary accession number(s): O24119
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: October 1, 2000
Last modified: November 26, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.