Q9LE15 (PER4_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Peroxidase 4
Short name=Atperox P4
EC=1.11.1.7

Alternative name(s):
ATP46

Gene names

Name:	PER4
Synonyms:	P4
Ordered Locus Names:	At1g14540
ORF Names:	T5E21.4, F14L17.32

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	315 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Removal of H2O2, oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.
Catalytic activity	2 phenolic donor + H2O2 = 2 phenoxyl radical of the donor + 2 H2O.
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per subunit By similarity. Binds 2 calcium ions per subunit By similarity.
Subcellular location	Secreted By similarity.
Miscellaneous	There are 73 peroxidase genes in A.thaliana.
Sequence similarities	Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Cellular component	Secreted
Domain	Signal
Ligand	Calcium Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
PTM	Disulfide bond Glycoprotein Pyrrolidone carboxylic acid
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cellular response to hypoxia Inferred from expression pattern. Source: TAIR hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	heme binding Inferred from electronic annotation. Source: InterPro peroxidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Chain	20 – 315	296	Peroxidase 4		PRO_0000023670
Sites
	Active site	61	1	Proton acceptor By similarity
	Metal binding	62	1	Calcium 1 By similarity
	Metal binding	65	1	Calcium 1; via carbonyl oxygen By similarity
	Metal binding	67	1	Calcium 1; via carbonyl oxygen By similarity
	Metal binding	69	1	Calcium 1 By similarity
	Metal binding	71	1	Calcium 1 By similarity
	Metal binding	188	1	Iron (heme axial ligand) By similarity
	Metal binding	189	1	Calcium 2 By similarity
	Metal binding	234	1	Calcium 2 By similarity
	Metal binding	237	1	Calcium 2 By similarity
	Metal binding	242	1	Calcium 2 By similarity
	Binding site	158	1	Substrate; via carbonyl oxygen By similarity
	Site	57	1	Transition state stabilizer By similarity
Amino acid modifications
	Modified residue	20	1	Pyrrolidone carboxylic acid By similarity
	Glycosylation	205	1	N-linked (GlcNAc...) Potential
	Disulfide bond	30 ↔ 110		By similarity
	Disulfide bond	63 ↔ 68		By similarity
	Disulfide bond	116 ↔ 311		By similarity
	Disulfide bond	195 ↔ 221		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9LE15 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 2DE6FA79CD16836D	FASTA	315	34,411
	10 20 30 40 50 60 MAIFKILVLL LSLCCFSQAQ LSPTFYDQTC QNALSTIRSS IRTAISRERR MAASLIRLHF 70 80 90 100 110 120 HDCFVNGCDA SVMLVATPTM ESERDSLANF QSARGFEVID QAKSAVESVC PGVVSCADII 130 140 150 160 170 180 AVAARDASEY VGGPRYDVKV GRRDSTNAFR AIADRDLPNF RASLNDLSEL FLRKGLNTRD 190 200 210 220 230 240 LVALSGAHTL GQAQCLTFKG RLYDNSSDID AGFSSTRKRR CPVNGGDTTL APLDQVTPNS 250 260 270 280 290 300 FDNNYYRNLM QKKGLLESDQ VLFGTGASTD SIVTEYSRNP SRFASDFSAA MIKMGDIQTL 310 TGSDGQIRRI CSAVN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Analysis and expression of the class III peroxidase large gene family in Arabidopsis thaliana." Tognolli M., Penel C., Greppin H., Simon P. Gene 288:129-138(2002) [PubMed: 12034502] [Abstract] Cited for: GENE FAMILY ORGANIZATION, NOMENCLATURE. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC010657 Genomic DNA. Translation: AAF63178.1. AC012188 Genomic DNA. Translation: AAF43954.1. CP002684 Genomic DNA. Translation: AEE29178.1.
IPI	IPI00521464.
RefSeq	NP_172906.1. NM_101321.3.
UniGene	At.41966.
3D structure databases
ProteinModelPortal	Q9LE15.
SMR	Q9LE15. Positions 20-315.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9LE15.
Protein family/group databases
PeroxiBase	80. AtPrx04.
Proteomic databases
PRIDE	Q9LE15.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G14540.1; AT1G14540.1; AT1G14540.
GeneID	838016.
GenomeReviews	Gene locus AT1G14540 in contig CT485782_GR.
KEGG	ath:AT1G14540.
NMPDR	fig|3702.1.peg.1739.
Organism-specific databases
GeneFarm	1503. 61.
TAIR	At1g14540.
Phylogenomic databases
GeneTree	EPGT00070000028277.
HOGENOM	HBG597790.
InParanoid	Q9LE15.
OMA	AISRERR.
PhylomeDB	Q9LE15.
ProtClustDB	CLSN2682957.
Gene expression databases
ArrayExpress	Q9LE15.
Genevestigator	Q9LE15.
GermOnline	AT1G14540. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR010255. Haem_peroxidase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR000823. Peroxidase_pln. IPR019794. Peroxidases_AS. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view]
KO	K00430.
Pfam	PF00141. peroxidase. 1 hit. [Graphical view]
PRINTS	PR00458. PEROXIDASE. PR00461. PLPEROXIDASE.
SUPFAM	SSF48113. Peroxidase_super. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. 1 hit. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PER4_ARATH

Accession

Primary (citable) accession number: Q9LE15

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2002
Last sequence update:	October 1, 2000
Last modified:	December 14, 2011
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program