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Q9LE06

- BCAT4_ARATH

UniProt

Q9LE06 - BCAT4_ARATH

Protein

Methionine aminotransferase BCAT4

Gene

BCAT4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 1 (01 Oct 2000)
      Previous versions | rss
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    Functioni

    Convert 2-oxo acids to branched-chain amino acids. Acts on methionine and its derivatives and the corresponding 2-oxo acids. Catalyzes the initial deamination of methionine to 4-methylthio-2-oxobutyrate as well as the transamination of other typical intermediates of the methionine chain elongation pathway.1 Publication

    Catalytic activityi

    L-methionine + a 2-oxo acid = 2-oxo-4-methylthiobutanoate + an L-amino acid.1 Publication

    Cofactori

    Pyridoxal phosphate.Curated

    Kineticsi

    1. KM=0.045 mM for 2-oxo acid 4-methylthio-2-oxobutyrate (MTOB)1 Publication
    2. KM=0.93 mM for methionine1 Publication
    3. KM=4.86 mM for leucine1 Publication

    Vmax=2.7 µmol/min/mg enzyme with MTOB as substrate1 Publication

    Vmax=0.089 µmol/min/mg enzyme with methionine as substrate1 Publication

    Vmax=0.11 µmol/min/mg enzyme with leucine as substrate1 Publication

    GO - Molecular functioni

    1. branched-chain-amino-acid transaminase activity Source: InterPro
    2. methionine-oxo-acid transaminase activity Source: TAIR

    GO - Biological processi

    1. branched-chain amino acid metabolic process Source: InterPro
    2. glucosinolate biosynthetic process Source: TAIR
    3. response to light stimulus Source: UniProtKB
    4. response to wounding Source: UniProtKB

    Keywords - Molecular functioni

    Aminotransferase, Transferase

    Keywords - Ligandi

    Pyridoxal phosphate

    Enzyme and pathway databases

    BioCyciARA:AT3G19710-MONOMER.
    MetaCyc:AT3G19710-MONOMER.
    BRENDAi2.6.1.42. 401.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Methionine aminotransferase BCAT4 (EC:2.6.1.88)
    Alternative name(s):
    Branched-chain-amino-acid aminotransferase 4
    Short name:
    Atbcat-4
    Methionine-oxo-acid transaminase BCAT4
    Gene namesi
    Name:BCAT4
    Ordered Locus Names:At3g19710
    ORF Names:MMB12_16
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 3

    Organism-specific databases

    TAIRiAT3G19710.

    Subcellular locationi

    Cytoplasm 2 Publications

    GO - Cellular componenti

    1. cytosol Source: TAIR

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Disruption phenotypei

    Reduced methionine-derived aliphatic glucosinolate accumulation, but increased levels of free methionine and S-methylmethionine.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 354354Methionine aminotransferase BCAT4PRO_0000103297Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei198 – 1981N6-(pyridoxal phosphate)lysineBy similarity

    Post-translational modificationi

    Ubiquitinated.1 Publication

    Keywords - PTMi

    Ubl conjugation

    Proteomic databases

    PaxDbiQ9LE06.
    PRIDEiQ9LE06.

    Expressioni

    Tissue specificityi

    Mostly expressed in phloem.1 Publication

    Inductioni

    Transient induction by wounding. Follows a diurnal rhythm.1 Publication

    Gene expression databases

    GenevestigatoriQ9LE06.

    Interactioni

    Protein-protein interaction databases

    BioGridi6841. 1 interaction.
    STRINGi3702.AT3G19710.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9LE06.
    SMRiQ9LE06. Positions 20-350.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0115.
    HOGENOMiHOG000276704.
    InParanoidiQ9LE06.
    KOiK00826.
    OMAiKWEELAF.
    PhylomeDBiQ9LE06.

    Family and domain databases

    InterProiIPR001544. Aminotrans_IV.
    IPR005786. B_amino_transII.
    [Graphical view]
    PANTHERiPTHR11825. PTHR11825. 1 hit.
    PfamiPF01063. Aminotran_4. 1 hit.
    [Graphical view]
    PIRSFiPIRSF006468. BCAT1. 1 hit.
    SUPFAMiSSF56752. SSF56752. 1 hit.
    TIGRFAMsiTIGR01123. ilvE_II. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9LE06-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAPSAQPLPV SVSDEKYANV KWEELAFKFV RTDYMYVAKC NHGESFQEGK    50
    ILPFADLQLN PCAAVLQYGQ GLYEGLKAYR TEDGRILLFR PDQNGLRLQA 100
    GADRLYMPYP SVDQFVSAIK QVALANKKWI PPPGKGTLYI RPILFGSGPI 150
    LGSFPIPETT FTAFACPVGR YHKDNSGLNL KIEDQFRRAF PSGTGGVKSI 200
    TNYCPVWIPL AEAKKQGFSD ILFLDAATGK NIEELFAANV FMLKGNVVST 250
    PTIAGTILPG VTRNCVMELC RDFGYQVEER TIPLVDFLDA DEAFCTGTAS 300
    IVTSIASVTF KDKKTGFKTG EETLAAKLYE TLSDIQTGRV EDTKGWTVEI 350
    DRQG 354
    Length:354
    Mass (Da):39,019
    Last modified:October 1, 2000 - v1
    Checksum:i313EA4C5B5AE1898
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ271732 mRNA. Translation: CAB93129.1.
    AP000417 Genomic DNA. Translation: BAB02558.1.
    CP002686 Genomic DNA. Translation: AEE76278.1.
    AY052676 mRNA. Translation: AAK96580.1.
    AF446892 mRNA. Translation: AAL38625.1.
    AK226525 mRNA. Translation: BAE98664.1.
    PIRiT52401.
    RefSeqiNP_188605.1. NM_112861.3.
    UniGeneiAt.22630.
    At.25237.

    Genome annotation databases

    EnsemblPlantsiAT3G19710.1; AT3G19710.1; AT3G19710.
    GeneIDi821508.
    KEGGiath:AT3G19710.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ271732 mRNA. Translation: CAB93129.1 .
    AP000417 Genomic DNA. Translation: BAB02558.1 .
    CP002686 Genomic DNA. Translation: AEE76278.1 .
    AY052676 mRNA. Translation: AAK96580.1 .
    AF446892 mRNA. Translation: AAL38625.1 .
    AK226525 mRNA. Translation: BAE98664.1 .
    PIRi T52401.
    RefSeqi NP_188605.1. NM_112861.3.
    UniGenei At.22630.
    At.25237.

    3D structure databases

    ProteinModelPortali Q9LE06.
    SMRi Q9LE06. Positions 20-350.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 6841. 1 interaction.
    STRINGi 3702.AT3G19710.1-P.

    Proteomic databases

    PaxDbi Q9LE06.
    PRIDEi Q9LE06.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT3G19710.1 ; AT3G19710.1 ; AT3G19710 .
    GeneIDi 821508.
    KEGGi ath:AT3G19710.

    Organism-specific databases

    TAIRi AT3G19710.

    Phylogenomic databases

    eggNOGi COG0115.
    HOGENOMi HOG000276704.
    InParanoidi Q9LE06.
    KOi K00826.
    OMAi KWEELAF.
    PhylomeDBi Q9LE06.

    Enzyme and pathway databases

    BioCyci ARA:AT3G19710-MONOMER.
    MetaCyc:AT3G19710-MONOMER.
    BRENDAi 2.6.1.42. 401.

    Gene expression databases

    Genevestigatori Q9LE06.

    Family and domain databases

    InterProi IPR001544. Aminotrans_IV.
    IPR005786. B_amino_transII.
    [Graphical view ]
    PANTHERi PTHR11825. PTHR11825. 1 hit.
    Pfami PF01063. Aminotran_4. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF006468. BCAT1. 1 hit.
    SUPFAMi SSF56752. SSF56752. 1 hit.
    TIGRFAMsi TIGR01123. ilvE_II. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The branched-chain amino acid transaminase gene family in Arabidopsis encodes plastid and mitochondrial proteins."
      Diebold R., Schuster J., Daschner K., Binder S.
      Plant Physiol. 129:540-550(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION.
      Strain: cv. Columbia.
    2. "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones."
      Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.
      DNA Res. 7:217-221(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
      Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
      , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
      Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    6. "Branched-chain aminotransferase4 is part of the chain elongation pathway in the biosynthesis of methionine-derived glucosinolates in Arabidopsis."
      Schuster J., Knill T., Reichelt M., Gershenzon J., Binder S.
      Plant Cell 18:2664-2679(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE, CATALYTIC ACTIVITY, INDUCTION BY WOUNDING, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, BIOPHYSICOCHEMICAL PROPERTIES.
    7. "Tandem affinity purification and mass spectrometric analysis of ubiquitylated proteins in Arabidopsis."
      Saracco S.A., Hansson M., Scalf M., Walker J.M., Smith L.M., Vierstra R.D.
      Plant J. 59:344-358(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiBCAT4_ARATH
    AccessioniPrimary (citable) accession number: Q9LE06
    Secondary accession number(s): Q0WW34
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 6, 2002
    Last sequence update: October 1, 2000
    Last modified: October 1, 2014
    This is version 105 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Although all the other members of the family possess a branched-chain amino acid aminotransferase activity, the exact function of BCAT4 remains unclear at present.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3