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Protein

Methionine aminotransferase BCAT4

Gene

BCAT4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Converts 2-oxo acids to branched-chain amino acids. Shows activity with L-Leu, L-Ile and L-Val as amino donors and alpha-keto-glutarate as an amino acceptor, but no activity for D-isomers of Leu, Ile, Val, Asp, Glu or Ala. Acts on methionine and its derivatives and the corresponding 2-oxo acids. Catalyzes the initial deamination of methionine to 4-methylthio-2-oxobutyrate as well as the transamination of other typical intermediates of the methionine chain elongation pathway.2 Publications

Catalytic activityi

L-methionine + a 2-oxo acid = 2-oxo-4-methylthiobutanoate + an L-amino acid.2 Publications

Cofactori

Kineticsi

  1. KM=0.045 mM for 2-oxo acid 4-methylthio-2-oxobutyrate (MTOB)1 Publication
  2. KM=0.93 mM for methionine1 Publication
  3. KM=4.86 mM for leucine1 Publication
  4. KM=1.61 mM for L-leucine1 Publication

Vmax=2.7 µmol/min/mg enzyme with MTOB as substrate1 Publication

Vmax=0.089 µmol/min/mg enzyme with methionine as substrate1 Publication

Vmax=0.11 µmol/min/mg enzyme with leucine as substrate1 Publication

GO - Molecular functioni

  1. branched-chain-amino-acid transaminase activity Source: InterPro
  2. methionine-oxo-acid transaminase activity Source: TAIR

GO - Biological processi

  1. branched-chain amino acid metabolic process Source: InterPro
  2. glucosinolate biosynthetic process Source: TAIR
  3. response to light stimulus Source: UniProtKB
  4. response to wounding Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciARA:AT3G19710-MONOMER.
MetaCyc:AT3G19710-MONOMER.
BRENDAi2.6.1.42. 401.
ReactomeiREACT_249002. Branched-chain amino acid catabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
Methionine aminotransferase BCAT4 (EC:2.6.1.88)
Alternative name(s):
Branched-chain-amino-acid aminotransferase 4
Short name:
Atbcat-4
Methionine-oxo-acid transaminase BCAT4
Gene namesi
Name:BCAT4
Ordered Locus Names:At3g19710
ORF Names:MMB12_16
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 3

Organism-specific databases

TAIRiAT3G19710.

Subcellular locationi

Cytoplasm 2 Publications

GO - Cellular componenti

  1. cytosol Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Reduced methionine-derived aliphatic glucosinolate accumulation, but increased levels of free methionine and S-methylmethionine.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 354354Methionine aminotransferase BCAT4PRO_0000103297Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei198 – 1981N6-(pyridoxal phosphate)lysineBy similarity

Post-translational modificationi

Ubiquitinated.1 Publication

Keywords - PTMi

Ubl conjugation

Proteomic databases

PaxDbiQ9LE06.
PRIDEiQ9LE06.

Expressioni

Tissue specificityi

Mostly expressed in phloem.1 Publication

Inductioni

Transient induction by wounding. Follows a diurnal rhythm.1 Publication

Gene expression databases

GenevestigatoriQ9LE06.

Interactioni

Protein-protein interaction databases

BioGridi6841. 1 interaction.
STRINGi3702.AT3G19710.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ9LE06.
SMRiQ9LE06. Positions 20-350.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0115.
HOGENOMiHOG000276704.
InParanoidiQ9LE06.
KOiK00826.
OMAiKWEELAF.
PhylomeDBiQ9LE06.

Family and domain databases

InterProiIPR001544. Aminotrans_IV.
IPR005786. B_amino_transII.
[Graphical view]
PANTHERiPTHR11825. PTHR11825. 1 hit.
PfamiPF01063. Aminotran_4. 1 hit.
[Graphical view]
PIRSFiPIRSF006468. BCAT1. 1 hit.
SUPFAMiSSF56752. SSF56752. 1 hit.
TIGRFAMsiTIGR01123. ilvE_II. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9LE06-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAPSAQPLPV SVSDEKYANV KWEELAFKFV RTDYMYVAKC NHGESFQEGK
60 70 80 90 100
ILPFADLQLN PCAAVLQYGQ GLYEGLKAYR TEDGRILLFR PDQNGLRLQA
110 120 130 140 150
GADRLYMPYP SVDQFVSAIK QVALANKKWI PPPGKGTLYI RPILFGSGPI
160 170 180 190 200
LGSFPIPETT FTAFACPVGR YHKDNSGLNL KIEDQFRRAF PSGTGGVKSI
210 220 230 240 250
TNYCPVWIPL AEAKKQGFSD ILFLDAATGK NIEELFAANV FMLKGNVVST
260 270 280 290 300
PTIAGTILPG VTRNCVMELC RDFGYQVEER TIPLVDFLDA DEAFCTGTAS
310 320 330 340 350
IVTSIASVTF KDKKTGFKTG EETLAAKLYE TLSDIQTGRV EDTKGWTVEI

DRQG
Length:354
Mass (Da):39,019
Last modified:October 1, 2000 - v1
Checksum:i313EA4C5B5AE1898
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ271732 mRNA. Translation: CAB93129.1.
AP000417 Genomic DNA. Translation: BAB02558.1.
CP002686 Genomic DNA. Translation: AEE76278.1.
AY052676 mRNA. Translation: AAK96580.1.
AF446892 mRNA. Translation: AAL38625.1.
AK226525 mRNA. Translation: BAE98664.1.
PIRiT52401.
RefSeqiNP_188605.1. NM_112861.3.
UniGeneiAt.22630.
At.25237.

Genome annotation databases

EnsemblPlantsiAT3G19710.1; AT3G19710.1; AT3G19710.
GeneIDi821508.
KEGGiath:AT3G19710.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ271732 mRNA. Translation: CAB93129.1.
AP000417 Genomic DNA. Translation: BAB02558.1.
CP002686 Genomic DNA. Translation: AEE76278.1.
AY052676 mRNA. Translation: AAK96580.1.
AF446892 mRNA. Translation: AAL38625.1.
AK226525 mRNA. Translation: BAE98664.1.
PIRiT52401.
RefSeqiNP_188605.1. NM_112861.3.
UniGeneiAt.22630.
At.25237.

3D structure databases

ProteinModelPortaliQ9LE06.
SMRiQ9LE06. Positions 20-350.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi6841. 1 interaction.
STRINGi3702.AT3G19710.1-P.

Proteomic databases

PaxDbiQ9LE06.
PRIDEiQ9LE06.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G19710.1; AT3G19710.1; AT3G19710.
GeneIDi821508.
KEGGiath:AT3G19710.

Organism-specific databases

TAIRiAT3G19710.

Phylogenomic databases

eggNOGiCOG0115.
HOGENOMiHOG000276704.
InParanoidiQ9LE06.
KOiK00826.
OMAiKWEELAF.
PhylomeDBiQ9LE06.

Enzyme and pathway databases

BioCyciARA:AT3G19710-MONOMER.
MetaCyc:AT3G19710-MONOMER.
BRENDAi2.6.1.42. 401.
ReactomeiREACT_249002. Branched-chain amino acid catabolism.

Gene expression databases

GenevestigatoriQ9LE06.

Family and domain databases

InterProiIPR001544. Aminotrans_IV.
IPR005786. B_amino_transII.
[Graphical view]
PANTHERiPTHR11825. PTHR11825. 1 hit.
PfamiPF01063. Aminotran_4. 1 hit.
[Graphical view]
PIRSFiPIRSF006468. BCAT1. 1 hit.
SUPFAMiSSF56752. SSF56752. 1 hit.
TIGRFAMsiTIGR01123. ilvE_II. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The branched-chain amino acid transaminase gene family in Arabidopsis encodes plastid and mitochondrial proteins."
    Diebold R., Schuster J., Daschner K., Binder S.
    Plant Physiol. 129:540-550(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION.
    Strain: cv. Columbia.
  2. "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones."
    Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.
    DNA Res. 7:217-221(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  6. "Branched-chain aminotransferase4 is part of the chain elongation pathway in the biosynthesis of methionine-derived glucosinolates in Arabidopsis."
    Schuster J., Knill T., Reichelt M., Gershenzon J., Binder S.
    Plant Cell 18:2664-2679(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE, CATALYTIC ACTIVITY, INDUCTION BY WOUNDING, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, BIOPHYSICOCHEMICAL PROPERTIES.
  7. "Cloning and functional characterization of Arabidopsis thaliana D-amino acid aminotransferase--D-aspartate behavior during germination."
    Funakoshi M., Sekine M., Katane M., Furuchi T., Yohda M., Yoshikawa T., Homma H.
    FEBS J. 275:1188-1200(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.
  8. "Tandem affinity purification and mass spectrometric analysis of ubiquitylated proteins in Arabidopsis."
    Saracco S.A., Hansson M., Scalf M., Walker J.M., Smith L.M., Vierstra R.D.
    Plant J. 59:344-358(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiBCAT4_ARATH
AccessioniPrimary (citable) accession number: Q9LE06
Secondary accession number(s): Q0WW34
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2002
Last sequence update: October 1, 2000
Last modified: January 7, 2015
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Although all the other members of the family possess a branched-chain amino acid aminotransferase activity, the exact function of BCAT4 remains unclear at present.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.