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Protein

Methionine aminotransferase BCAT4

Gene

BCAT4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Converts 2-oxo acids to branched-chain amino acids. Shows activity with L-Leu, L-Ile and L-Val as amino donors and alpha-keto-glutarate as an amino acceptor, but no activity for D-isomers of Leu, Ile, Val, Asp, Glu or Ala. Acts on methionine and its derivatives and the corresponding 2-oxo acids. Catalyzes the initial deamination of methionine to 4-methylthio-2-oxobutyrate as well as the transamination of other typical intermediates of the methionine chain elongation pathway.2 Publications

Catalytic activityi

L-methionine + a 2-oxo acid = 2-oxo-4-methylthiobutanoate + an L-amino acid.2 Publications

Cofactori

Kineticsi

  1. KM=0.045 mM for 2-oxo acid 4-methylthio-2-oxobutyrate (MTOB)1 Publication
  2. KM=0.93 mM for methionine1 Publication
  3. KM=4.86 mM for leucine1 Publication
  4. KM=1.61 mM for L-leucine1 Publication
  1. Vmax=2.7 µmol/min/mg enzyme with MTOB as substrate1 Publication
  2. Vmax=0.089 µmol/min/mg enzyme with methionine as substrate1 Publication
  3. Vmax=0.11 µmol/min/mg enzyme with leucine as substrate1 Publication

GO - Molecular functioni

GO - Biological processi

  • aspartate biosynthetic process Source: GO_Central
  • branched-chain amino acid metabolic process Source: InterPro
  • glucosinolate biosynthetic process Source: TAIR
  • leucine biosynthetic process Source: GO_Central
  • response to light stimulus Source: UniProtKB
  • response to wounding Source: UniProtKB
  • valine biosynthetic process Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciARA:AT3G19710-MONOMER.
MetaCyc:AT3G19710-MONOMER.
BRENDAi2.6.1.88. 399.
ReactomeiR-ATH-70895. Branched-chain amino acid catabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
Methionine aminotransferase BCAT4 (EC:2.6.1.88)
Alternative name(s):
Branched-chain-amino-acid aminotransferase 4
Short name:
Atbcat-4
Methionine-oxo-acid transaminase BCAT4
Gene namesi
Name:BCAT4
Ordered Locus Names:At3g19710
ORF Names:MMB12_16
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G19710.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Reduced methionine-derived aliphatic glucosinolate accumulation, but increased levels of free methionine and S-methylmethionine.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001032971 – 354Methionine aminotransferase BCAT4Add BLAST354

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei198N6-(pyridoxal phosphate)lysineBy similarity1

Proteomic databases

PaxDbiQ9LE06.
PRIDEiQ9LE06.

PTM databases

iPTMnetiQ9LE06.

Expressioni

Tissue specificityi

Mostly expressed in phloem.1 Publication

Inductioni

Transient induction by wounding. Follows a diurnal rhythm.1 Publication

Gene expression databases

GenevisibleiQ9LE06. AT.

Interactioni

Protein-protein interaction databases

BioGridi6841. 1 interactor.
STRINGi3702.AT3G19710.1.

Structurei

3D structure databases

ProteinModelPortaliQ9LE06.
SMRiQ9LE06.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0975. Eukaryota.
COG0115. LUCA.
HOGENOMiHOG000276704.
InParanoidiQ9LE06.
KOiK00826.
OMAiGRYHKDN.
OrthoDBiEOG09360CSJ.
PhylomeDBiQ9LE06.

Family and domain databases

CDDicd01557. BCAT_beta_family. 1 hit.
InterProiIPR001544. Aminotrans_IV.
IPR005786. B_amino_transII.
IPR033939. BCAT_family.
[Graphical view]
PANTHERiPTHR11825. PTHR11825. 1 hit.
PfamiPF01063. Aminotran_4. 1 hit.
[Graphical view]
PIRSFiPIRSF006468. BCAT1. 1 hit.
SUPFAMiSSF56752. SSF56752. 1 hit.
TIGRFAMsiTIGR01123. ilvE_II. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9LE06-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPSAQPLPV SVSDEKYANV KWEELAFKFV RTDYMYVAKC NHGESFQEGK
60 70 80 90 100
ILPFADLQLN PCAAVLQYGQ GLYEGLKAYR TEDGRILLFR PDQNGLRLQA
110 120 130 140 150
GADRLYMPYP SVDQFVSAIK QVALANKKWI PPPGKGTLYI RPILFGSGPI
160 170 180 190 200
LGSFPIPETT FTAFACPVGR YHKDNSGLNL KIEDQFRRAF PSGTGGVKSI
210 220 230 240 250
TNYCPVWIPL AEAKKQGFSD ILFLDAATGK NIEELFAANV FMLKGNVVST
260 270 280 290 300
PTIAGTILPG VTRNCVMELC RDFGYQVEER TIPLVDFLDA DEAFCTGTAS
310 320 330 340 350
IVTSIASVTF KDKKTGFKTG EETLAAKLYE TLSDIQTGRV EDTKGWTVEI

DRQG
Length:354
Mass (Da):39,019
Last modified:October 1, 2000 - v1
Checksum:i313EA4C5B5AE1898
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ271732 mRNA. Translation: CAB93129.1.
AP000417 Genomic DNA. Translation: BAB02558.1.
CP002686 Genomic DNA. Translation: AEE76278.1.
AY052676 mRNA. Translation: AAK96580.1.
AF446892 mRNA. Translation: AAL38625.1.
AK226525 mRNA. Translation: BAE98664.1.
PIRiT52401.
RefSeqiNP_188605.1. NM_112861.4.
UniGeneiAt.22630.
At.25237.

Genome annotation databases

EnsemblPlantsiAT3G19710.1; AT3G19710.1; AT3G19710.
GeneIDi821508.
GrameneiAT3G19710.1; AT3G19710.1; AT3G19710.
KEGGiath:AT3G19710.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ271732 mRNA. Translation: CAB93129.1.
AP000417 Genomic DNA. Translation: BAB02558.1.
CP002686 Genomic DNA. Translation: AEE76278.1.
AY052676 mRNA. Translation: AAK96580.1.
AF446892 mRNA. Translation: AAL38625.1.
AK226525 mRNA. Translation: BAE98664.1.
PIRiT52401.
RefSeqiNP_188605.1. NM_112861.4.
UniGeneiAt.22630.
At.25237.

3D structure databases

ProteinModelPortaliQ9LE06.
SMRiQ9LE06.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi6841. 1 interactor.
STRINGi3702.AT3G19710.1.

PTM databases

iPTMnetiQ9LE06.

Proteomic databases

PaxDbiQ9LE06.
PRIDEiQ9LE06.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G19710.1; AT3G19710.1; AT3G19710.
GeneIDi821508.
GrameneiAT3G19710.1; AT3G19710.1; AT3G19710.
KEGGiath:AT3G19710.

Organism-specific databases

TAIRiAT3G19710.

Phylogenomic databases

eggNOGiKOG0975. Eukaryota.
COG0115. LUCA.
HOGENOMiHOG000276704.
InParanoidiQ9LE06.
KOiK00826.
OMAiGRYHKDN.
OrthoDBiEOG09360CSJ.
PhylomeDBiQ9LE06.

Enzyme and pathway databases

BioCyciARA:AT3G19710-MONOMER.
MetaCyc:AT3G19710-MONOMER.
BRENDAi2.6.1.88. 399.
ReactomeiR-ATH-70895. Branched-chain amino acid catabolism.

Miscellaneous databases

PROiQ9LE06.

Gene expression databases

GenevisibleiQ9LE06. AT.

Family and domain databases

CDDicd01557. BCAT_beta_family. 1 hit.
InterProiIPR001544. Aminotrans_IV.
IPR005786. B_amino_transII.
IPR033939. BCAT_family.
[Graphical view]
PANTHERiPTHR11825. PTHR11825. 1 hit.
PfamiPF01063. Aminotran_4. 1 hit.
[Graphical view]
PIRSFiPIRSF006468. BCAT1. 1 hit.
SUPFAMiSSF56752. SSF56752. 1 hit.
TIGRFAMsiTIGR01123. ilvE_II. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiBCAT4_ARATH
AccessioniPrimary (citable) accession number: Q9LE06
Secondary accession number(s): Q0WW34
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2002
Last sequence update: October 1, 2000
Last modified: November 30, 2016
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Although all the other members of the family possess a branched-chain amino acid aminotransferase activity, the exact function of BCAT4 remains unclear at present.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.