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Protein

Methionine aminotransferase BCAT4

Gene

BCAT4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Converts 2-oxo acids to branched-chain amino acids. Shows activity with L-Leu, L-Ile and L-Val as amino donors and alpha-keto-glutarate as an amino acceptor, but no activity for D-isomers of Leu, Ile, Val, Asp, Glu or Ala. Acts on methionine and its derivatives and the corresponding 2-oxo acids. Catalyzes the initial deamination of methionine to 4-methylthio-2-oxobutyrate as well as the transamination of other typical intermediates of the methionine chain elongation pathway.2 Publications

Miscellaneous

Although all the other members of the family possess a branched-chain amino acid aminotransferase activity, the exact function of BCAT4 remains unclear at present.

Catalytic activityi

L-methionine + a 2-oxo acid = 2-oxo-4-methylthiobutanoate + an L-amino acid.2 Publications

Cofactori

Kineticsi

  1. KM=0.045 mM for 2-oxo acid 4-methylthio-2-oxobutyrate (MTOB)1 Publication
  2. KM=0.93 mM for methionine1 Publication
  3. KM=4.86 mM for leucine1 Publication
  4. KM=1.61 mM for L-leucine1 Publication
  1. Vmax=2.7 µmol/min/mg enzyme with MTOB as substrate1 Publication
  2. Vmax=0.089 µmol/min/mg enzyme with methionine as substrate1 Publication
  3. Vmax=0.11 µmol/min/mg enzyme with leucine as substrate1 Publication

GO - Molecular functioni

GO - Biological processi

  • branched-chain amino acid metabolic process Source: InterPro
  • glucosinolate biosynthetic process Source: TAIR
  • response to light stimulus Source: UniProtKB
  • response to wounding Source: UniProtKB

Keywordsi

Molecular functionAminotransferase, Transferase
LigandPyridoxal phosphate

Enzyme and pathway databases

BioCyciARA:AT3G19710-MONOMER
MetaCyc:AT3G19710-MONOMER
BRENDAi2.6.1.88 399
ReactomeiR-ATH-70895 Branched-chain amino acid catabolism

Names & Taxonomyi

Protein namesi
Recommended name:
Methionine aminotransferase BCAT4 (EC:2.6.1.88)
Alternative name(s):
Branched-chain-amino-acid aminotransferase 4
Short name:
Atbcat-4
Methionine-oxo-acid transaminase BCAT4
Gene namesi
Name:BCAT4
Ordered Locus Names:At3g19710
ORF Names:MMB12_16
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G19710
TAIRilocus:2091216 AT3G19710

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Reduced methionine-derived aliphatic glucosinolate accumulation, but increased levels of free methionine and S-methylmethionine.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001032971 – 354Methionine aminotransferase BCAT4Add BLAST354

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei198N6-(pyridoxal phosphate)lysineBy similarity1

Proteomic databases

PaxDbiQ9LE06
PRIDEiQ9LE06

PTM databases

iPTMnetiQ9LE06

Expressioni

Tissue specificityi

Mostly expressed in phloem.1 Publication

Inductioni

Transient induction by wounding. Follows a diurnal rhythm.1 Publication

Gene expression databases

ExpressionAtlasiQ9LE06 baseline and differential
GenevisibleiQ9LE06 AT

Interactioni

Protein-protein interaction databases

BioGridi6841, 1 interactor
STRINGi3702.AT3G19710.1

Structurei

3D structure databases

ProteinModelPortaliQ9LE06
SMRiQ9LE06
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0975 Eukaryota
COG0115 LUCA
HOGENOMiHOG000276704
InParanoidiQ9LE06
KOiK21346
OMAiRYHKDNS
OrthoDBiEOG09360CSJ
PhylomeDBiQ9LE06

Family and domain databases

CDDicd01557 BCAT_beta_family, 1 hit
InterProiView protein in InterPro
IPR001544 Aminotrans_IV
IPR036038 Aminotransferase-like
IPR005786 B_amino_transII
IPR033939 BCAT_family
PfamiView protein in Pfam
PF01063 Aminotran_4, 1 hit
PIRSFiPIRSF006468 BCAT1, 1 hit
SUPFAMiSSF56752 SSF56752, 1 hit
TIGRFAMsiTIGR01123 ilvE_II, 1 hit

Sequencei

Sequence statusi: Complete.

Q9LE06-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPSAQPLPV SVSDEKYANV KWEELAFKFV RTDYMYVAKC NHGESFQEGK
60 70 80 90 100
ILPFADLQLN PCAAVLQYGQ GLYEGLKAYR TEDGRILLFR PDQNGLRLQA
110 120 130 140 150
GADRLYMPYP SVDQFVSAIK QVALANKKWI PPPGKGTLYI RPILFGSGPI
160 170 180 190 200
LGSFPIPETT FTAFACPVGR YHKDNSGLNL KIEDQFRRAF PSGTGGVKSI
210 220 230 240 250
TNYCPVWIPL AEAKKQGFSD ILFLDAATGK NIEELFAANV FMLKGNVVST
260 270 280 290 300
PTIAGTILPG VTRNCVMELC RDFGYQVEER TIPLVDFLDA DEAFCTGTAS
310 320 330 340 350
IVTSIASVTF KDKKTGFKTG EETLAAKLYE TLSDIQTGRV EDTKGWTVEI

DRQG
Length:354
Mass (Da):39,019
Last modified:October 1, 2000 - v1
Checksum:i313EA4C5B5AE1898
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ271732 mRNA Translation: CAB93129.1
AP000417 Genomic DNA Translation: BAB02558.1
CP002686 Genomic DNA Translation: AEE76278.1
AY052676 mRNA Translation: AAK96580.1
AF446892 mRNA Translation: AAL38625.1
AK226525 mRNA Translation: BAE98664.1
PIRiT52401
RefSeqiNP_188605.1, NM_112861.4
UniGeneiAt.22630
At.25237

Genome annotation databases

EnsemblPlantsiAT3G19710.1; AT3G19710.1; AT3G19710
GeneIDi821508
GrameneiAT3G19710.1; AT3G19710.1; AT3G19710
KEGGiath:AT3G19710

Similar proteinsi

Entry informationi

Entry nameiBCAT4_ARATH
AccessioniPrimary (citable) accession number: Q9LE06
Secondary accession number(s): Q0WW34
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 6, 2002
Last sequence update: October 1, 2000
Last modified: April 25, 2018
This is version 130 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health