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Q9LDX1

- SGS3_ARATH

UniProt

Q9LDX1 - SGS3_ARATH

Protein

Protein SUPPRESSOR OF GENE SILENCING 3

Gene

SGS3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 68 (01 Oct 2014)
      Sequence version 1 (01 Oct 2000)
      Previous versions | rss
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    Functioni

    Required for post-transcriptional gene silencing and natural virus resistance. May bind nucleic acids and is essential for the biogenesis of trans-acting siRNAs but is not required for silencing induced by IR-PTGS. Involved in the juvenile-to-adult transition regulation. In case of begomoviruses infection, it is targeted by the viral protein V2 leading to suppression of post-transcriptional gene silencing.2 Publications

    GO - Biological processi

    1. defense response to virus Source: TAIR
    2. production of ta-siRNAs involved in RNA interference Source: TAIR
    3. regulation of defense response to virus Source: UniProtKB-KW
    4. vegetative phase change Source: TAIR
    5. viral process Source: UniProtKB-KW
    6. virus induced gene silencing Source: TAIR
    7. wax biosynthetic process Source: TAIR

    Keywords - Molecular functioni

    Antiviral protein

    Keywords - Biological processi

    Host-virus interaction, RNA-mediated gene silencing

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein SUPPRESSOR OF GENE SILENCING 3
    Short name:
    AtSGS3
    Gene namesi
    Name:SGS3
    Ordered Locus Names:At5g23570
    ORF Names:MQM1.17
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G23570.

    Subcellular locationi

    Cytoplasmperinuclear region 1 Publication
    Note: Accumulates in inclusion bodies in the cell periphery. May interact with the ER network from the perinuclear region out to the cell periphery By similarity.By similarity

    GO - Cellular componenti

    1. cytoplasm Source: TAIR
    2. cytosol Source: TAIR
    3. endoplasmic reticulum Source: TAIR
    4. perinuclear region of cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi500 – 5001E → K in sgs3-3; complete suppression of post-transcriptional gene silencing. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 625625Protein SUPPRESSOR OF GENE SILENCING 3PRO_0000333289Add
    BLAST

    Proteomic databases

    PaxDbiQ9LDX1.
    PRIDEiQ9LDX1.

    Expressioni

    Gene expression databases

    GenevestigatoriQ9LDX1.

    Interactioni

    Subunit structurei

    Interacts with begomoviruses protein V2.1 Publication

    Protein-protein interaction databases

    DIPiDIP-29658N.
    IntActiQ9LDX1. 1 interaction.
    MINTiMINT-7014693.
    STRINGi3702.AT5G23570.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9LDX1.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili452 – 53382Sequence AnalysisAdd
    BLAST
    Coiled coili564 – 61552Sequence AnalysisAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi75 – 11137Gly-richAdd
    BLAST
    Compositional biasi156 – 18934Asp-richAdd
    BLAST
    Compositional biasi540 – 5434Poly-Gln

    Sequence similaritiesi

    Belongs to the SGS3 family.Curated

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG259720.
    HOGENOMiHOG000085166.
    InParanoidiQ9LDX1.
    OMAiNQHSQGK.
    PhylomeDBiQ9LDX1.

    Family and domain databases

    InterProiIPR005380. XS_domain.
    IPR005381. Znf-XS_domain.
    [Graphical view]
    PfamiPF03468. XS. 1 hit.
    PF03470. zf-XS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9LDX1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSSRAGPMSK EKNVQGGYRP EVEQLVQGLA GTRLASSQDD GGEWEVISKK    50
    NKNKPGNTSG KTWVSQNSNP PRAWGGQQQG RGSNVSGRGN NVSGRGNGNG 100
    RGIQANISGR GRALSRKYDN NFVAPPPVSR PPLEGGWNWQ ARGGSAQHTA 150
    VQEFPDVEDD VDNASEEEND SDALDDSDDD LASDDYDSDV SQKSHGSRKQ 200
    NKWFKKFFGS LDSLSIEQIN EPQRQWHCPA CQNGPGAIDW YNLHPLLAHA 250
    RTKGARRVKL HRELAEVLEK DLQMRGASVI PCGEIYGQWK GLGEDEKDYE 300
    IVWPPMVIIM NTRLDKDDND KWLGMGNQEL LEYFDKYEAL RARHSYGPQG 350
    HRGMSVLMFE SSATGYLEAE RLHRELAEMG LDRIAWGQKR SMFSGGVRQL 400
    YGFLATKQDL DIFNQHSQGK TRLKFELKSY QEMVVKELRQ ISEDNQQLNY 450
    FKNKLSKQNK HAKVLEESLE IMSEKLRRTA EDNRIVRQRT KMQHEQNREE 500
    MDAHDRFFMD SIKQIHERRD AKEENFEMLQ QQERAKVVGQ QQQNINPSSN 550
    DDCRKRAEEV SSFIEFQEKE MEEFVEEREM LIKDQEKKME DMKKRHHEEI 600
    FDLEKEFDEA LEQLMYKHGL HNEDD 625
    Length:625
    Mass (Da):71,972
    Last modified:October 1, 2000 - v1
    Checksum:i456E2A1396706A96
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF239719 Genomic DNA. Translation: AAF73960.1.
    AB025633 Genomic DNA. Translation: BAA97244.1.
    CP002688 Genomic DNA. Translation: AED93184.1.
    BT002944 mRNA. Translation: AAO22757.1.
    BT004380 mRNA. Translation: AAO42374.1.
    RefSeqiNP_197747.1. NM_122263.2.
    UniGeneiAt.31001.

    Genome annotation databases

    EnsemblPlantsiAT5G23570.1; AT5G23570.1; AT5G23570.
    GeneIDi832422.
    KEGGiath:AT5G23570.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF239719 Genomic DNA. Translation: AAF73960.1 .
    AB025633 Genomic DNA. Translation: BAA97244.1 .
    CP002688 Genomic DNA. Translation: AED93184.1 .
    BT002944 mRNA. Translation: AAO22757.1 .
    BT004380 mRNA. Translation: AAO42374.1 .
    RefSeqi NP_197747.1. NM_122263.2.
    UniGenei At.31001.

    3D structure databases

    ProteinModelPortali Q9LDX1.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-29658N.
    IntActi Q9LDX1. 1 interaction.
    MINTi MINT-7014693.
    STRINGi 3702.AT5G23570.1-P.

    Proteomic databases

    PaxDbi Q9LDX1.
    PRIDEi Q9LDX1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G23570.1 ; AT5G23570.1 ; AT5G23570 .
    GeneIDi 832422.
    KEGGi ath:AT5G23570.

    Organism-specific databases

    TAIRi AT5G23570.

    Phylogenomic databases

    eggNOGi NOG259720.
    HOGENOMi HOG000085166.
    InParanoidi Q9LDX1.
    OMAi NQHSQGK.
    PhylomeDBi Q9LDX1.

    Gene expression databases

    Genevestigatori Q9LDX1.

    Family and domain databases

    InterProi IPR005380. XS_domain.
    IPR005381. Znf-XS_domain.
    [Graphical view ]
    Pfami PF03468. XS. 1 hit.
    PF03470. zf-XS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Arabidopsis SGS2 and SGS3 genes are required for posttranscriptional gene silencing and natural virus resistance."
      Mourrain P., Beclin C., Elmayan T., Feuerbach F., Godon C., Morel J.-B., Jouette D., Lacombe A.-M., Nikic S., Picault N., Remoue K., Sanial M., Vo T.-A., Vaucheret H.
      Cell 101:533-542(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF GLU-500.
    2. "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones."
      Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S.
      DNA Res. 7:31-63(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "The SGS3 protein involved in PTGS finds a family."
      Bateman A.
      BMC Bioinformatics 3:21-21(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY.
    6. "SGS3 and SGS2/SDE1/RDR6 are required for juvenile development and the production of trans-acting siRNAs in Arabidopsis."
      Peragine A., Yoshikawa M., Wu G., Albrecht H.L., Poethig R.S.
      Genes Dev. 18:2368-2379(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "Geminivirus VIGS of endogenous genes requires SGS2/SDE1 and SGS3 and defines a new branch in the genetic pathway for silencing in plants."
      Muangsan N., Beclin C., Vaucheret H., Robertson D.
      Plant J. 38:1004-1014(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    8. "Interaction with host SGS3 is required for suppression of RNA silencing by tomato yellow leaf curl virus V2 protein."
      Glick E., Zrachya A., Levy Y., Mett A., Gidoni D., Belausov E., Citovsky V., Gafni Y.
      Proc. Natl. Acad. Sci. U.S.A. 105:157-161(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, INTERACTION WITH TOMATO YELLOW LEAF CURL VIRUS PROTEIN V2.

    Entry informationi

    Entry nameiSGS3_ARATH
    AccessioniPrimary (citable) accession number: Q9LDX1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 20, 2008
    Last sequence update: October 1, 2000
    Last modified: October 1, 2014
    This is version 68 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3