Protein SUPPRESSOR OF GENE SILENCING 3 - Arabidopsis thaliana (Mouse-ear cress)

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Q9LDX1 (SGS3_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 61. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Protein SUPPRESSOR OF GENE SILENCING 3
Short name=AtSGS3

Gene names

Name:	SGS3
Ordered Locus Names:	At5g23570
ORF Names:	MQM1.17

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	625 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for post-transcriptional gene silencing and natural virus resistance. May bind nucleic acids and is essential for the biogenesis of trans-acting siRNAs but is not required for silencing induced by IR-PTGS. Involved in the juvenile-to-adult transition regulation. In case of begomoviruses infection, it is targeted by the viral protein V2 leading to suppression of post-transcriptional gene silencing. Ref.6 Ref.7
Subunit structure	Interacts with begomoviruses protein V2. Ref.8
Subcellular location	Cytoplasm › perinuclear region. Note: Accumulates in inclusion bodies in the cell periphery. May interact with the ER network from the perinuclear region out to the cell periphery By similarity. Ref.8
Sequence similarities	Belongs to the SGS3 family.

Ontologies

Keywords
Biological process	Host-virus interaction RNA-mediated gene silencing
Cellular component	Cytoplasm
Domain	Coiled coil
Molecular function	Antiviral protein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	production of ta-siRNAs involved in RNA interference Inferred from mutant phenotype PubMed 16131612 PubMed 16682354. Source: TAIR regulation of defense response to virus Inferred from electronic annotation. Source: UniProtKB-KW vegetative phase change Inferred from mutant phenotype Ref.6. Source: TAIR virus induced gene silencing Inferred from mutant phenotype Ref.1. Source: TAIR virus-host interaction Inferred from electronic annotation. Source: UniProtKB-KW wax biosynthetic process Inferred from genetic interaction PubMed 22689894. Source: TAIR
Cellular_component	cytosol Inferred from direct assay PubMed 21166475. Source: TAIR endoplasmic reticulum Inferred from direct assay PubMed 22689894. Source: TAIR perinuclear region of cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 625

625

Protein SUPPRESSOR OF GENE SILENCING 3

PRO_0000333289

Regions

Coiled coil

452 – 533

Potential

Coiled coil

564 – 615

Potential

Compositional bias

75 – 111

Gly-rich

Compositional bias

156 – 189

Asp-rich

Compositional bias

540 – 543

Poly-Gln

Experimental info

Mutagenesis

500

E → K in sgs3-3; complete suppression of post-transcriptional gene silencing. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

Q9LDX1 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 456E2A1396706A96
Show »

FASTA

625

71,972

        10         20         30         40         50         60 
MSSRAGPMSK EKNVQGGYRP EVEQLVQGLA GTRLASSQDD GGEWEVISKK NKNKPGNTSG 

        70         80         90        100        110        120 
KTWVSQNSNP PRAWGGQQQG RGSNVSGRGN NVSGRGNGNG RGIQANISGR GRALSRKYDN 

       130        140        150        160        170        180 
NFVAPPPVSR PPLEGGWNWQ ARGGSAQHTA VQEFPDVEDD VDNASEEEND SDALDDSDDD 

       190        200        210        220        230        240 
LASDDYDSDV SQKSHGSRKQ NKWFKKFFGS LDSLSIEQIN EPQRQWHCPA CQNGPGAIDW 

       250        260        270        280        290        300 
YNLHPLLAHA RTKGARRVKL HRELAEVLEK DLQMRGASVI PCGEIYGQWK GLGEDEKDYE 

       310        320        330        340        350        360 
IVWPPMVIIM NTRLDKDDND KWLGMGNQEL LEYFDKYEAL RARHSYGPQG HRGMSVLMFE 

       370        380        390        400        410        420 
SSATGYLEAE RLHRELAEMG LDRIAWGQKR SMFSGGVRQL YGFLATKQDL DIFNQHSQGK 

       430        440        450        460        470        480 
TRLKFELKSY QEMVVKELRQ ISEDNQQLNY FKNKLSKQNK HAKVLEESLE IMSEKLRRTA 

       490        500        510        520        530        540 
EDNRIVRQRT KMQHEQNREE MDAHDRFFMD SIKQIHERRD AKEENFEMLQ QQERAKVVGQ 

       550        560        570        580        590        600 
QQQNINPSSN DDCRKRAEEV SSFIEFQEKE MEEFVEEREM LIKDQEKKME DMKKRHHEEI 

       610        620 
FDLEKEFDEA LEQLMYKHGL HNEDD

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Arabidopsis SGS2 and SGS3 genes are required for posttranscriptional gene silencing and natural virus resistance." Mourrain P., Beclin C., Elmayan T., Feuerbach F., Godon C., Morel J.-B., Jouette D., Lacombe A.-M., Nikic S., Picault N., Remoue K., Sanial M., Vo T.-A., Vaucheret H. Cell 101:533-542(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF GLU-500.
[2]	"Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones." Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S. DNA Res. 7:31-63(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"The SGS3 protein involved in PTGS finds a family." Bateman A. BMC Bioinformatics 3:21-21(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY.
[6]	"SGS3 and SGS2/SDE1/RDR6 are required for juvenile development and the production of trans-acting siRNAs in Arabidopsis." Peragine A., Yoshikawa M., Wu G., Albrecht H.L., Poethig R.S. Genes Dev. 18:2368-2379(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[7]	"Geminivirus VIGS of endogenous genes requires SGS2/SDE1 and SGS3 and defines a new branch in the genetic pathway for silencing in plants." Muangsan N., Beclin C., Vaucheret H., Robertson D. Plant J. 38:1004-1014(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[8]	"Interaction with host SGS3 is required for suppression of RNA silencing by tomato yellow leaf curl virus V2 protein." Glick E., Zrachya A., Levy Y., Mett A., Gidoni D., Belausov E., Citovsky V., Gafni Y. Proc. Natl. Acad. Sci. U.S.A. 105:157-161(2008) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, INTERACTION WITH TOMATO YELLOW LEAF CURL VIRUS PROTEIN V2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF239719 Genomic DNA. Translation: AAF73960.1. AB025633 Genomic DNA. Translation: BAA97244.1. CP002688 Genomic DNA. Translation: AED93184.1. BT002944 mRNA. Translation: AAO22757.1. BT004380 mRNA. Translation: AAO42374.1.
IPI	IPI00531176.
RefSeq	NP_197747.1. NM_122263.2.
UniGene	At.31001.
3D structure databases
ProteinModelPortal	Q9LDX1.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-29658N.
MINT	MINT-7014693.
STRING	3702.AT5G23570.1-P.
Proteomic databases
PaxDb	Q9LDX1.
PRIDE	Q9LDX1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G23570.1; AT5G23570.1; AT5G23570.
GeneID	832422.
KEGG	ath:AT5G23570.
Organism-specific databases
TAIR	At5g23570.
Phylogenomic databases
eggNOG	NOG259720.
HOGENOM	HOG000085166.
InParanoid	Q9LDX1.
OMA	PPMVIIM.
PhylomeDB	Q9LDX1.
ProtClustDB	CLSN2914848.
Gene expression databases
Genevestigator	Q9LDX1.
Family and domain databases
InterPro	IPR005380. XS_domain. IPR005381. Znf-XS_domain. [Graphical view]
Pfam	PF03468. XS. 1 hit. PF03470. zf-XS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SGS3_ARATH

Accession

Primary (citable) accession number: Q9LDX1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	October 1, 2000
Last modified:	May 1, 2013
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents