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Protein

Protein SUPPRESSOR OF GENE SILENCING 3

Gene

SGS3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for post-transcriptional gene silencing and natural virus resistance. May bind nucleic acids and is essential for the biogenesis of trans-acting siRNAs but is not required for silencing induced by IR-PTGS. Involved in the juvenile-to-adult transition regulation. In case of begomoviruses infection, it is targeted by the viral protein V2 leading to suppression of post-transcriptional gene silencing.2 Publications

GO - Molecular functioni

  1. ribonuclease P activity Source: GO_Central

GO - Biological processi

  1. defense response to virus Source: TAIR
  2. production of ta-siRNAs involved in RNA interference Source: TAIR
  3. regulation of defense response to virus Source: UniProtKB-KW
  4. RNA phosphodiester bond hydrolysis, endonucleolytic Source: GO_Central
  5. vegetative phase change Source: TAIR
  6. viral process Source: UniProtKB-KW
  7. virus induced gene silencing Source: TAIR
  8. wax biosynthetic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Antiviral protein

Keywords - Biological processi

Host-virus interaction, RNA-mediated gene silencing

Names & Taxonomyi

Protein namesi
Recommended name:
Protein SUPPRESSOR OF GENE SILENCING 3
Short name:
AtSGS3
Gene namesi
Name:SGS3
Ordered Locus Names:At5g23570
ORF Names:MQM1.17
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G23570.

Subcellular locationi

  1. Cytoplasmperinuclear region 1 Publication

  2. Note: Accumulates in inclusion bodies in the cell periphery. May interact with the ER network from the perinuclear region out to the cell periphery (By similarity).By similarity

GO - Cellular componenti

  1. cytoplasm Source: TAIR
  2. cytosol Source: TAIR
  3. endoplasmic reticulum Source: TAIR
  4. nucleolar ribonuclease P complex Source: GO_Central
  5. perinuclear region of cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi500 – 5001E → K in sgs3-3; complete suppression of post-transcriptional gene silencing. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 625625Protein SUPPRESSOR OF GENE SILENCING 3PRO_0000333289Add
BLAST

Proteomic databases

PaxDbiQ9LDX1.
PRIDEiQ9LDX1.

Expressioni

Gene expression databases

GenevestigatoriQ9LDX1.

Interactioni

Subunit structurei

Interacts with begomoviruses protein V2.1 Publication

Protein-protein interaction databases

DIPiDIP-29658N.
IntActiQ9LDX1. 1 interaction.
MINTiMINT-7014693.
STRINGi3702.AT5G23570.1-P.

Structurei

3D structure databases

SMRiQ9LDX1. Positions 422-463.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili452 – 53382Sequence AnalysisAdd
BLAST
Coiled coili564 – 61552Sequence AnalysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi75 – 11137Gly-richAdd
BLAST
Compositional biasi156 – 18934Asp-richAdd
BLAST
Compositional biasi540 – 5434Poly-Gln

Sequence similaritiesi

Belongs to the SGS3 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG259720.
HOGENOMiHOG000085166.
InParanoidiQ9LDX1.
OMAiSKARHAY.
PhylomeDBiQ9LDX1.

Family and domain databases

InterProiIPR005380. XS_domain.
IPR005381. Znf-XS_domain.
[Graphical view]
PfamiPF03468. XS. 1 hit.
PF03470. zf-XS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9LDX1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSRAGPMSK EKNVQGGYRP EVEQLVQGLA GTRLASSQDD GGEWEVISKK
60 70 80 90 100
NKNKPGNTSG KTWVSQNSNP PRAWGGQQQG RGSNVSGRGN NVSGRGNGNG
110 120 130 140 150
RGIQANISGR GRALSRKYDN NFVAPPPVSR PPLEGGWNWQ ARGGSAQHTA
160 170 180 190 200
VQEFPDVEDD VDNASEEEND SDALDDSDDD LASDDYDSDV SQKSHGSRKQ
210 220 230 240 250
NKWFKKFFGS LDSLSIEQIN EPQRQWHCPA CQNGPGAIDW YNLHPLLAHA
260 270 280 290 300
RTKGARRVKL HRELAEVLEK DLQMRGASVI PCGEIYGQWK GLGEDEKDYE
310 320 330 340 350
IVWPPMVIIM NTRLDKDDND KWLGMGNQEL LEYFDKYEAL RARHSYGPQG
360 370 380 390 400
HRGMSVLMFE SSATGYLEAE RLHRELAEMG LDRIAWGQKR SMFSGGVRQL
410 420 430 440 450
YGFLATKQDL DIFNQHSQGK TRLKFELKSY QEMVVKELRQ ISEDNQQLNY
460 470 480 490 500
FKNKLSKQNK HAKVLEESLE IMSEKLRRTA EDNRIVRQRT KMQHEQNREE
510 520 530 540 550
MDAHDRFFMD SIKQIHERRD AKEENFEMLQ QQERAKVVGQ QQQNINPSSN
560 570 580 590 600
DDCRKRAEEV SSFIEFQEKE MEEFVEEREM LIKDQEKKME DMKKRHHEEI
610 620
FDLEKEFDEA LEQLMYKHGL HNEDD
Length:625
Mass (Da):71,972
Last modified:October 1, 2000 - v1
Checksum:i456E2A1396706A96
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF239719 Genomic DNA. Translation: AAF73960.1.
AB025633 Genomic DNA. Translation: BAA97244.1.
CP002688 Genomic DNA. Translation: AED93184.1.
BT002944 mRNA. Translation: AAO22757.1.
BT004380 mRNA. Translation: AAO42374.1.
RefSeqiNP_197747.1. NM_122263.2.
UniGeneiAt.31001.

Genome annotation databases

EnsemblPlantsiAT5G23570.1; AT5G23570.1; AT5G23570.
GeneIDi832422.
KEGGiath:AT5G23570.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF239719 Genomic DNA. Translation: AAF73960.1.
AB025633 Genomic DNA. Translation: BAA97244.1.
CP002688 Genomic DNA. Translation: AED93184.1.
BT002944 mRNA. Translation: AAO22757.1.
BT004380 mRNA. Translation: AAO42374.1.
RefSeqiNP_197747.1. NM_122263.2.
UniGeneiAt.31001.

3D structure databases

SMRiQ9LDX1. Positions 422-463.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-29658N.
IntActiQ9LDX1. 1 interaction.
MINTiMINT-7014693.
STRINGi3702.AT5G23570.1-P.

Proteomic databases

PaxDbiQ9LDX1.
PRIDEiQ9LDX1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G23570.1; AT5G23570.1; AT5G23570.
GeneIDi832422.
KEGGiath:AT5G23570.

Organism-specific databases

TAIRiAT5G23570.

Phylogenomic databases

eggNOGiNOG259720.
HOGENOMiHOG000085166.
InParanoidiQ9LDX1.
OMAiSKARHAY.
PhylomeDBiQ9LDX1.

Miscellaneous databases

PROiQ9LDX1.

Gene expression databases

GenevestigatoriQ9LDX1.

Family and domain databases

InterProiIPR005380. XS_domain.
IPR005381. Znf-XS_domain.
[Graphical view]
PfamiPF03468. XS. 1 hit.
PF03470. zf-XS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Arabidopsis SGS2 and SGS3 genes are required for posttranscriptional gene silencing and natural virus resistance."
    Mourrain P., Beclin C., Elmayan T., Feuerbach F., Godon C., Morel J.-B., Jouette D., Lacombe A.-M., Nikic S., Picault N., Remoue K., Sanial M., Vo T.-A., Vaucheret H.
    Cell 101:533-542(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF GLU-500.
  2. "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones."
    Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S.
    DNA Res. 7:31-63(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "The SGS3 protein involved in PTGS finds a family."
    Bateman A.
    BMC Bioinformatics 3:21-21(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY.
  6. "SGS3 and SGS2/SDE1/RDR6 are required for juvenile development and the production of trans-acting siRNAs in Arabidopsis."
    Peragine A., Yoshikawa M., Wu G., Albrecht H.L., Poethig R.S.
    Genes Dev. 18:2368-2379(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Geminivirus VIGS of endogenous genes requires SGS2/SDE1 and SGS3 and defines a new branch in the genetic pathway for silencing in plants."
    Muangsan N., Beclin C., Vaucheret H., Robertson D.
    Plant J. 38:1004-1014(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "Interaction with host SGS3 is required for suppression of RNA silencing by tomato yellow leaf curl virus V2 protein."
    Glick E., Zrachya A., Levy Y., Mett A., Gidoni D., Belausov E., Citovsky V., Gafni Y.
    Proc. Natl. Acad. Sci. U.S.A. 105:157-161(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, INTERACTION WITH TOMATO YELLOW LEAF CURL VIRUS PROTEIN V2.

Entry informationi

Entry nameiSGS3_ARATH
AccessioniPrimary (citable) accession number: Q9LDX1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: October 1, 2000
Last modified: April 29, 2015
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.