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Q9LDX1 (SGS3_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Protein SUPPRESSOR OF GENE SILENCING 3

Short name=AtSGS3
Gene names
Name:SGS3
Ordered Locus Names:At5g23570
ORF Names:MQM1.17
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length625 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for post-transcriptional gene silencing and natural virus resistance. May bind nucleic acids and is essential for the biogenesis of trans-acting siRNAs but is not required for silencing induced by IR-PTGS. Involved in the juvenile-to-adult transition regulation. In case of begomoviruses infection, it is targeted by the viral protein V2 leading to suppression of post-transcriptional gene silencing. Ref.6 Ref.7

Subunit structure

Interacts with begomoviruses protein V2. Ref.8

Subcellular location

Cytoplasmperinuclear region. Note: Accumulates in inclusion bodies in the cell periphery. May interact with the ER network from the perinuclear region out to the cell periphery By similarity. Ref.8

Sequence similarities

Belongs to the SGS3 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 625625Protein SUPPRESSOR OF GENE SILENCING 3
PRO_0000333289

Regions

Coiled coil452 – 53382 Potential
Coiled coil564 – 61552 Potential
Compositional bias75 – 11137Gly-rich
Compositional bias156 – 18934Asp-rich
Compositional bias540 – 5434Poly-Gln

Experimental info

Mutagenesis5001E → K in sgs3-3; complete suppression of post-transcriptional gene silencing. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q9LDX1 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 456E2A1396706A96

FASTA62571,972
        10         20         30         40         50         60 
MSSRAGPMSK EKNVQGGYRP EVEQLVQGLA GTRLASSQDD GGEWEVISKK NKNKPGNTSG 

        70         80         90        100        110        120 
KTWVSQNSNP PRAWGGQQQG RGSNVSGRGN NVSGRGNGNG RGIQANISGR GRALSRKYDN 

       130        140        150        160        170        180 
NFVAPPPVSR PPLEGGWNWQ ARGGSAQHTA VQEFPDVEDD VDNASEEEND SDALDDSDDD 

       190        200        210        220        230        240 
LASDDYDSDV SQKSHGSRKQ NKWFKKFFGS LDSLSIEQIN EPQRQWHCPA CQNGPGAIDW 

       250        260        270        280        290        300 
YNLHPLLAHA RTKGARRVKL HRELAEVLEK DLQMRGASVI PCGEIYGQWK GLGEDEKDYE 

       310        320        330        340        350        360 
IVWPPMVIIM NTRLDKDDND KWLGMGNQEL LEYFDKYEAL RARHSYGPQG HRGMSVLMFE 

       370        380        390        400        410        420 
SSATGYLEAE RLHRELAEMG LDRIAWGQKR SMFSGGVRQL YGFLATKQDL DIFNQHSQGK 

       430        440        450        460        470        480 
TRLKFELKSY QEMVVKELRQ ISEDNQQLNY FKNKLSKQNK HAKVLEESLE IMSEKLRRTA 

       490        500        510        520        530        540 
EDNRIVRQRT KMQHEQNREE MDAHDRFFMD SIKQIHERRD AKEENFEMLQ QQERAKVVGQ 

       550        560        570        580        590        600 
QQQNINPSSN DDCRKRAEEV SSFIEFQEKE MEEFVEEREM LIKDQEKKME DMKKRHHEEI 

       610        620 
FDLEKEFDEA LEQLMYKHGL HNEDD 

« Hide

References

« Hide 'large scale' references
[1]"Arabidopsis SGS2 and SGS3 genes are required for posttranscriptional gene silencing and natural virus resistance."
Mourrain P., Beclin C., Elmayan T., Feuerbach F., Godon C., Morel J.-B., Jouette D., Lacombe A.-M., Nikic S., Picault N., Remoue K., Sanial M., Vo T.-A., Vaucheret H.
Cell 101:533-542(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF GLU-500.
[2]"Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones."
Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S.
DNA Res. 7:31-63(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"The SGS3 protein involved in PTGS finds a family."
Bateman A.
BMC Bioinformatics 3:21-21(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY.
[6]"SGS3 and SGS2/SDE1/RDR6 are required for juvenile development and the production of trans-acting siRNAs in Arabidopsis."
Peragine A., Yoshikawa M., Wu G., Albrecht H.L., Poethig R.S.
Genes Dev. 18:2368-2379(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[7]"Geminivirus VIGS of endogenous genes requires SGS2/SDE1 and SGS3 and defines a new branch in the genetic pathway for silencing in plants."
Muangsan N., Beclin C., Vaucheret H., Robertson D.
Plant J. 38:1004-1014(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[8]"Interaction with host SGS3 is required for suppression of RNA silencing by tomato yellow leaf curl virus V2 protein."
Glick E., Zrachya A., Levy Y., Mett A., Gidoni D., Belausov E., Citovsky V., Gafni Y.
Proc. Natl. Acad. Sci. U.S.A. 105:157-161(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, INTERACTION WITH TOMATO YELLOW LEAF CURL VIRUS PROTEIN V2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF239719 Genomic DNA. Translation: AAF73960.1.
AB025633 Genomic DNA. Translation: BAA97244.1.
CP002688 Genomic DNA. Translation: AED93184.1.
BT002944 mRNA. Translation: AAO22757.1.
BT004380 mRNA. Translation: AAO42374.1.
RefSeqNP_197747.1. NM_122263.2.
UniGeneAt.31001.

3D structure databases

ProteinModelPortalQ9LDX1.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-29658N.
IntActQ9LDX1. 1 interaction.
MINTMINT-7014693.
STRING3702.AT5G23570.1-P.

Proteomic databases

PaxDbQ9LDX1.
PRIDEQ9LDX1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G23570.1; AT5G23570.1; AT5G23570.
GeneID832422.
KEGGath:AT5G23570.

Organism-specific databases

TAIRAT5G23570.

Phylogenomic databases

eggNOGNOG259720.
HOGENOMHOG000085166.
InParanoidQ9LDX1.
OMANQHSQGK.
PhylomeDBQ9LDX1.

Gene expression databases

GenevestigatorQ9LDX1.

Family and domain databases

InterProIPR005380. XS_domain.
IPR005381. Znf-XS_domain.
[Graphical view]
PfamPF03468. XS. 1 hit.
PF03470. zf-XS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSGS3_ARATH
AccessionPrimary (citable) accession number: Q9LDX1
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: October 1, 2000
Last modified: May 14, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names