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Q9LDV4

- ALAT2_ARATH

UniProt

Q9LDV4 - ALAT2_ARATH

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Protein
Alanine aminotransferase 2, mitochondrial
Gene
ALAAT2, AOAT3, At1g72330, T10D10.20, T9N14.10
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalytic activityi

L-alanine + 2-oxoglutarate = pyruvate + L-glutamate.

Cofactori

Pyridoxal phosphate By similarity.

Pathwayi

GO - Molecular functioni

  1. L-alanine:2-oxoglutarate aminotransferase activity Source: UniProtKB-EC
  2. pyridoxal phosphate binding Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. L-alanine catabolic process Source: UniProtKB-UniPathway
  2. biosynthetic process Source: InterPro
  3. response to hypoxia Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciARA:GQT-1694-MONOMER.
ARA:GQT-1695-MONOMER.
UniPathwayiUPA00322.
UPA00528; UER00586.

Names & Taxonomyi

Protein namesi
Recommended name:
Alanine aminotransferase 2, mitochondrial (EC:2.6.1.2)
Short name:
AtAlaAT2
Short name:
AtAlaATm
Alternative name(s):
Alanine-2-oxoglutarate aminotransferase 3 (EC:2.6.1.-)
Gene namesi
Name:ALAAT2
Synonyms:AOAT3
Ordered Locus Names:At1g72330
ORF Names:T10D10.20, T9N14.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G72330.

Subcellular locationi

Mitochondrion Reviewed prediction

GO - Cellular componenti

  1. mitochondrion Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 4646Mitochondrion Reviewed prediction
Add
BLAST
Chaini47 – 540494Alanine aminotransferase 2, mitochondrial
PRO_0000416043Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei357 – 3571N6-(pyridoxal phosphate)lysine By similarity

Post-translational modificationi

The N-terminus is blocked By similarity.

Proteomic databases

PRIDEiQ9LDV4.

Expressioni

Tissue specificityi

Expressed in shoots, essentially in leaves and flowers, mostly in vascular tissues. Also detected in stems and roots.2 Publications

Inductioni

Rapidly induced upon low-oxygen stress in roots and shoots.1 Publication

Gene expression databases

GenevestigatoriQ9LDV4.

Interactioni

Subunit structurei

Homodimer By similarity.

Protein-protein interaction databases

STRINGi3702.AT1G72330.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ9LDV4.
SMRiQ9LDV4. Positions 63-539.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

HOGENOMiHOG000215020.
InParanoidiQ9LDV4.
KOiK00814.
PhylomeDBiQ9LDV4.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00155. Aminotran_1_2. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 2 hits.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9LDV4-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MRRFLINQAK GLVDHSRRQH HHKSPSFLSP QPRPLASSPP ALSRFFSSTS    50
EMSASDSTSS LPVTLDSINP KVLKCEYAVR GEIVNIAQKL QEDLKTNKDA 100
YPFDEIIYCN IGNPQSLGQL PIKFFREVLA LCDHASLLDE SETHGLFSTD 150
SIDRAWRILD HIPGRATGAY SHSQGIKGLR DVIAAGIEAR DGFPADPNDI 200
FLTDGASPAV HMMMQLLLSS EKDGILSPIP QYPLYSASIA LHGGSLVPYY 250
LDEATGWGLE ISDLKKQLEE ARSKGISVRA LVVINPGNPT GQVLAEENQR 300
DIVNFCKQEG LVLLADEVYQ ENVYVPDKKF HSFKKVARSL GYGEKDISLV 350
SFQSVSKGYY GECGKRGGYM EVTGFTSDVR EQIYKMASVN LCSNISGQIL 400
ASLVMSPPKP GDDSYDSYMA ERDGILSSMA KRAKTLEDAL NSLEGVTCNR 450
AEGAMYLFPR INLPQKAIEA AEAEKTAPDA FYCKRLLNAT GVVVVPGSGF 500
GQVPGTWHFR CTILPQEDKI PAIVNRLTEF HKSFMDEFRN 540
Length:540
Mass (Da):59,511
Last modified:October 1, 2000 - v1
Checksum:i4D1D4D4102CBD661
GO
Isoform 2 (identifier: Q9LDV4-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     410-540: PGDDSYDSYM...HKSFMDEFRN → KEMEFSHPWLNVQRLWKTLSTV

Note: Derived from EST data. No experimental confirmation available.

Show »
Length:431
Mass (Da):47,710
Checksum:i62385DCABF0834D1
GO

Sequence cautioni

The sequence AAG51787.1 differs from that shown. Reason: Erroneous gene model prediction.
The sequence AEE35307.1 differs from that shown. Reason: Erroneous gene model prediction.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei410 – 540131PGDDS…DEFRN → KEMEFSHPWLNVQRLWKTLS TV in isoform 2.
VSP_042467Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti265 – 2651K → E in AAK59591. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF275371 mRNA. Translation: AAF82781.1.
AC016529 Genomic DNA. Translation: AAG52580.1.
AC067754 Genomic DNA. Translation: AAG51787.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE35305.1.
CP002684 Genomic DNA. Translation: AEE35306.1.
CP002684 Genomic DNA. Translation: AEE35307.1. Sequence problems.
AY035086 mRNA. Translation: AAK59591.2.
PIRiB96747.
RefSeqiNP_001077811.1. NM_001084342.1. [Q9LDV4-2]
NP_001185380.1. NM_001198451.1.
NP_565040.2. NM_105892.4. [Q9LDV4-1]
UniGeneiAt.15579.

Genome annotation databases

EnsemblPlantsiAT1G72330.1; AT1G72330.1; AT1G72330. [Q9LDV4-1]
GeneIDi843565.
KEGGiath:AT1G72330.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF275371 mRNA. Translation: AAF82781.1 .
AC016529 Genomic DNA. Translation: AAG52580.1 .
AC067754 Genomic DNA. Translation: AAG51787.1 . Sequence problems.
CP002684 Genomic DNA. Translation: AEE35305.1 .
CP002684 Genomic DNA. Translation: AEE35306.1 .
CP002684 Genomic DNA. Translation: AEE35307.1 . Sequence problems.
AY035086 mRNA. Translation: AAK59591.2 .
PIRi B96747.
RefSeqi NP_001077811.1. NM_001084342.1. [Q9LDV4-2 ]
NP_001185380.1. NM_001198451.1.
NP_565040.2. NM_105892.4. [Q9LDV4-1 ]
UniGenei At.15579.

3D structure databases

ProteinModelPortali Q9LDV4.
SMRi Q9LDV4. Positions 63-539.
ModBasei Search...

Protein-protein interaction databases

STRINGi 3702.AT1G72330.1-P.

Proteomic databases

PRIDEi Q9LDV4.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT1G72330.1 ; AT1G72330.1 ; AT1G72330 . [Q9LDV4-1 ]
GeneIDi 843565.
KEGGi ath:AT1G72330.

Organism-specific databases

TAIRi AT1G72330.

Phylogenomic databases

HOGENOMi HOG000215020.
InParanoidi Q9LDV4.
KOi K00814.
PhylomeDBi Q9LDV4.

Enzyme and pathway databases

UniPathwayi UPA00322 .
UPA00528 ; UER00586 .
BioCyci ARA:GQT-1694-MONOMER.
ARA:GQT-1695-MONOMER.

Gene expression databases

Genevestigatori Q9LDV4.

Family and domain databases

Gene3Di 3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProi IPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view ]
Pfami PF00155. Aminotran_1_2. 1 hit.
[Graphical view ]
SUPFAMi SSF53383. SSF53383. 2 hits.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of Arabidopsis alanine aminotransferase genes."
    Ismond K.P., Dennis E.S., Dolferus R.
    Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Strain: cv. C24.
  2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 9-540 (ISOFORM 1).
    Strain: cv. Columbia.
  5. "Identification of photorespiratory glutamate:glyoxylate aminotransferase (GGAT) gene in Arabidopsis."
    Igarashi D., Miwa T., Seki M., Kobayashi M., Kato T., Tabata S., Shinozaki K., Ohsumi C.
    Plant J. 33:975-987(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, GENE FAMILY, NOMENCLATURE.
  6. "Alanine aminotransferase catalyses the breakdown of alanine after hypoxia in Arabidopsis thaliana."
    Miyashita Y., Dolferus R., Ismond K.P., Good A.G.
    Plant J. 49:1108-1121(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, INDUCTION BY HYPOXIC STRESS.

Entry informationi

Entry nameiALAT2_ARATH
AccessioniPrimary (citable) accession number: Q9LDV4
Secondary accession number(s): F4IDA2
, F4IDA4, Q94C83, Q9C7S4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 21, 2012
Last sequence update: October 1, 2000
Last modified: May 14, 2014
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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