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Q9LDV4

- ALAT2_ARATH

UniProt

Q9LDV4 - ALAT2_ARATH

Protein

Alanine aminotransferase 2, mitochondrial

Gene

ALAAT2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 90 (01 Oct 2014)
      Sequence version 1 (01 Oct 2000)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    L-alanine + 2-oxoglutarate = pyruvate + L-glutamate.

    Cofactori

    Pyridoxal phosphate.By similarity

    Pathwayi

    GO - Molecular functioni

    1. L-alanine:2-oxoglutarate aminotransferase activity Source: UniProtKB-EC
    2. pyridoxal phosphate binding Source: InterPro

    GO - Biological processi

    1. biosynthetic process Source: InterPro
    2. L-alanine catabolic process Source: UniProtKB-UniPathway
    3. response to hypoxia Source: UniProtKB

    Keywords - Molecular functioni

    Aminotransferase, Transferase

    Keywords - Ligandi

    Pyridoxal phosphate

    Enzyme and pathway databases

    BioCyciARA:GQT-1694-MONOMER.
    ARA:GQT-1695-MONOMER.
    UniPathwayiUPA00322.
    UPA00528; UER00586.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alanine aminotransferase 2, mitochondrial (EC:2.6.1.2)
    Short name:
    AtAlaAT2
    Short name:
    AtAlaATm
    Alternative name(s):
    Alanine-2-oxoglutarate aminotransferase 3 (EC:2.6.1.-)
    Gene namesi
    Name:ALAAT2
    Synonyms:AOAT3
    Ordered Locus Names:At1g72330
    ORF Names:T10D10.20, T9N14.10
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G72330.

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrion Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 4646MitochondrionSequence AnalysisAdd
    BLAST
    Chaini47 – 540494Alanine aminotransferase 2, mitochondrialPRO_0000416043Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei357 – 3571N6-(pyridoxal phosphate)lysineBy similarity

    Post-translational modificationi

    The N-terminus is blocked.By similarity

    Proteomic databases

    PRIDEiQ9LDV4.

    Expressioni

    Tissue specificityi

    Expressed in shoots, essentially in leaves and flowers, mostly in vascular tissues. Also detected in stems and roots.2 Publications

    Inductioni

    Rapidly induced upon low-oxygen stress in roots and shoots.1 Publication

    Gene expression databases

    GenevestigatoriQ9LDV4.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    STRINGi3702.AT1G72330.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9LDV4.
    SMRiQ9LDV4. Positions 63-539.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    HOGENOMiHOG000215020.
    InParanoidiQ9LDV4.
    KOiK00814.
    PhylomeDBiQ9LDV4.

    Family and domain databases

    Gene3Di3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    InterProiIPR004839. Aminotransferase_I/II.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    [Graphical view]
    PfamiPF00155. Aminotran_1_2. 1 hit.
    [Graphical view]
    SUPFAMiSSF53383. SSF53383. 2 hits.

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9LDV4-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MRRFLINQAK GLVDHSRRQH HHKSPSFLSP QPRPLASSPP ALSRFFSSTS    50
    EMSASDSTSS LPVTLDSINP KVLKCEYAVR GEIVNIAQKL QEDLKTNKDA 100
    YPFDEIIYCN IGNPQSLGQL PIKFFREVLA LCDHASLLDE SETHGLFSTD 150
    SIDRAWRILD HIPGRATGAY SHSQGIKGLR DVIAAGIEAR DGFPADPNDI 200
    FLTDGASPAV HMMMQLLLSS EKDGILSPIP QYPLYSASIA LHGGSLVPYY 250
    LDEATGWGLE ISDLKKQLEE ARSKGISVRA LVVINPGNPT GQVLAEENQR 300
    DIVNFCKQEG LVLLADEVYQ ENVYVPDKKF HSFKKVARSL GYGEKDISLV 350
    SFQSVSKGYY GECGKRGGYM EVTGFTSDVR EQIYKMASVN LCSNISGQIL 400
    ASLVMSPPKP GDDSYDSYMA ERDGILSSMA KRAKTLEDAL NSLEGVTCNR 450
    AEGAMYLFPR INLPQKAIEA AEAEKTAPDA FYCKRLLNAT GVVVVPGSGF 500
    GQVPGTWHFR CTILPQEDKI PAIVNRLTEF HKSFMDEFRN 540
    Length:540
    Mass (Da):59,511
    Last modified:October 1, 2000 - v1
    Checksum:i4D1D4D4102CBD661
    GO
    Isoform 2 (identifier: Q9LDV4-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         410-540: PGDDSYDSYM...HKSFMDEFRN → KEMEFSHPWLNVQRLWKTLSTV

    Note: Derived from EST data. No experimental confirmation available.

    Show »
    Length:431
    Mass (Da):47,710
    Checksum:i62385DCABF0834D1
    GO

    Sequence cautioni

    The sequence AAG51787.1 differs from that shown. Reason: Erroneous gene model prediction.
    The sequence AEE35307.1 differs from that shown. Reason: Erroneous gene model prediction.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti265 – 2651K → E in AAK59591. (PubMed:14593172)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei410 – 540131PGDDS…DEFRN → KEMEFSHPWLNVQRLWKTLS TV in isoform 2. CuratedVSP_042467Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF275371 mRNA. Translation: AAF82781.1.
    AC016529 Genomic DNA. Translation: AAG52580.1.
    AC067754 Genomic DNA. Translation: AAG51787.1. Sequence problems.
    CP002684 Genomic DNA. Translation: AEE35305.1.
    CP002684 Genomic DNA. Translation: AEE35306.1.
    CP002684 Genomic DNA. Translation: AEE35307.1. Sequence problems.
    AY035086 mRNA. Translation: AAK59591.2.
    PIRiB96747.
    RefSeqiNP_001077811.1. NM_001084342.1. [Q9LDV4-2]
    NP_001185380.1. NM_001198451.1.
    NP_565040.2. NM_105892.4. [Q9LDV4-1]
    UniGeneiAt.15579.

    Genome annotation databases

    EnsemblPlantsiAT1G72330.1; AT1G72330.1; AT1G72330. [Q9LDV4-1]
    GeneIDi843565.
    KEGGiath:AT1G72330.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF275371 mRNA. Translation: AAF82781.1 .
    AC016529 Genomic DNA. Translation: AAG52580.1 .
    AC067754 Genomic DNA. Translation: AAG51787.1 . Sequence problems.
    CP002684 Genomic DNA. Translation: AEE35305.1 .
    CP002684 Genomic DNA. Translation: AEE35306.1 .
    CP002684 Genomic DNA. Translation: AEE35307.1 . Sequence problems.
    AY035086 mRNA. Translation: AAK59591.2 .
    PIRi B96747.
    RefSeqi NP_001077811.1. NM_001084342.1. [Q9LDV4-2 ]
    NP_001185380.1. NM_001198451.1.
    NP_565040.2. NM_105892.4. [Q9LDV4-1 ]
    UniGenei At.15579.

    3D structure databases

    ProteinModelPortali Q9LDV4.
    SMRi Q9LDV4. Positions 63-539.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 3702.AT1G72330.1-P.

    Proteomic databases

    PRIDEi Q9LDV4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G72330.1 ; AT1G72330.1 ; AT1G72330 . [Q9LDV4-1 ]
    GeneIDi 843565.
    KEGGi ath:AT1G72330.

    Organism-specific databases

    TAIRi AT1G72330.

    Phylogenomic databases

    HOGENOMi HOG000215020.
    InParanoidi Q9LDV4.
    KOi K00814.
    PhylomeDBi Q9LDV4.

    Enzyme and pathway databases

    UniPathwayi UPA00322 .
    UPA00528 ; UER00586 .
    BioCyci ARA:GQT-1694-MONOMER.
    ARA:GQT-1695-MONOMER.

    Gene expression databases

    Genevestigatori Q9LDV4.

    Family and domain databases

    Gene3Di 3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    InterProi IPR004839. Aminotransferase_I/II.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    [Graphical view ]
    Pfami PF00155. Aminotran_1_2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53383. SSF53383. 2 hits.
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and characterization of Arabidopsis alanine aminotransferase genes."
      Ismond K.P., Dennis E.S., Dolferus R.
      Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Strain: cv. C24.
    2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 9-540 (ISOFORM 1).
      Strain: cv. Columbia.
    5. "Identification of photorespiratory glutamate:glyoxylate aminotransferase (GGAT) gene in Arabidopsis."
      Igarashi D., Miwa T., Seki M., Kobayashi M., Kato T., Tabata S., Shinozaki K., Ohsumi C.
      Plant J. 33:975-987(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, GENE FAMILY, NOMENCLATURE.
    6. "Alanine aminotransferase catalyses the breakdown of alanine after hypoxia in Arabidopsis thaliana."
      Miyashita Y., Dolferus R., Ismond K.P., Good A.G.
      Plant J. 49:1108-1121(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, INDUCTION BY HYPOXIC STRESS.

    Entry informationi

    Entry nameiALAT2_ARATH
    AccessioniPrimary (citable) accession number: Q9LDV4
    Secondary accession number(s): F4IDA2
    , F4IDA4, Q94C83, Q9C7S4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 21, 2012
    Last sequence update: October 1, 2000
    Last modified: October 1, 2014
    This is version 90 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3