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Protein

7-dehydrocholesterol reductase

Gene

DWF5

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Production of cholesterol by reduction of C7-C8 double bond of 7-dehydrocholesterol (7-DHC). Lesions in the gene coding for the enzyme cause dwarfism.

Catalytic activityi

Cholesterol + NADP+ = cholesta-5,7-dien-3-beta-ol + NADPH.

Pathwayi: steroid biosynthesis

This protein is involved in the pathway steroid biosynthesis, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway steroid biosynthesis and in Lipid metabolism.

GO - Molecular functioni

  • 7-dehydrocholesterol reductase activity Source: UniProtKB-EC
  • sterol delta7 reductase activity Source: TAIR

GO - Biological processi

  • brassinosteroid biosynthetic process Source: TAIR
  • cholesterol biosynthetic process Source: UniProtKB-KW

Keywordsi

Molecular functionOxidoreductase
Biological processCholesterol biosynthesis, Cholesterol metabolism, Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism
LigandNADP

Enzyme and pathway databases

BioCyciARA:AT1G50430-MONOMER
MetaCyc:AT1G50430-MONOMER
BRENDAi1.3.1.21 399
ReactomeiR-ATH-6807047 Cholesterol biosynthesis via desmosterol
R-ATH-6807062 Cholesterol biosynthesis via lathosterol
UniPathwayiUPA00062

Names & Taxonomyi

Protein namesi
Recommended name:
7-dehydrocholesterol reductase (EC:1.3.1.21)
Short name:
7-DHC reductase
Alternative name(s):
Protein DWARF 5
Sterol Delta(7)-reductase
Gene namesi
Name:DWF5
Synonyms:ST7R
Ordered Locus Names:At1g50430
ORF Names:F11F12.21
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G50430
TAIRilocus:2008071 AT1G50430

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei12 – 34HelicalSequence analysisAdd BLAST23
Transmembranei64 – 86HelicalSequence analysisAdd BLAST23
Transmembranei107 – 126HelicalSequence analysisAdd BLAST20
Transmembranei136 – 155HelicalSequence analysisAdd BLAST20
Transmembranei195 – 212HelicalSequence analysisAdd BLAST18
Transmembranei227 – 249HelicalSequence analysisAdd BLAST23
Transmembranei261 – 283HelicalSequence analysisAdd BLAST23
Transmembranei287 – 309HelicalSequence analysisAdd BLAST23
Transmembranei371 – 393HelicalSequence analysisAdd BLAST23

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi257D → N in DWF5-4; dwarf plant. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002075091 – 4327-dehydrocholesterol reductaseAdd BLAST432

Proteomic databases

PaxDbiQ9LDU6

PTM databases

iPTMnetiQ9LDU6
SwissPalmiQ9LDU6

Expressioni

Gene expression databases

ExpressionAtlasiQ9LDU6 baseline and differential
GenevisibleiQ9LDU6 AT

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
NIP1-1Q8VZW12EBI-4439540,EBI-4424378

Protein-protein interaction databases

BioGridi26690, 5 interactors
IntActiQ9LDU6, 5 interactors
STRINGi3702.AT1G50430.1

Structurei

3D structure databases

ProteinModelPortaliQ9LDU6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ERG4/ERG24 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1435 Eukaryota
ENOG410XP67 LUCA
HOGENOMiHOG000193296
InParanoidiQ9LDU6
KOiK00213
OMAiTNHQKDL
OrthoDBiEOG093609EP
PhylomeDBiQ9LDU6

Family and domain databases

InterProiView protein in InterPro
IPR001171 Ergosterol_biosynth_ERG4_ERG24
IPR018083 Sterol_reductase_CS
PfamiView protein in Pfam
PF01222 ERG4_ERG24, 1 hit
PROSITEiView protein in PROSITE
PS01017 STEROL_REDUCT_1, 1 hit
PS01018 STEROL_REDUCT_2, 1 hit

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q9LDU6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAETVHSPIV TYASMLSLLA FCPPFVILLW YTMVHQDGSV TQTFGFFWEN
60 70 80 90 100
GVQGLINIWP RPTLIAWKII FCYGAFEAIL QLLLPGKRVE GPISPAGNRP
110 120 130 140 150
VYKANGLAAY FVTLATYLGL WWFGIFNPAI VYDHLGEIFS ALIFGSFIFC
160 170 180 190 200
VLLYIKGHVA PSSSDSGSCG NLIIDFYWGM ELYPRIGKSF DIKVFTNCRF
210 220 230 240 250
GMMSWAVLAV TYCIKQYEIN GKVSDSMLVN TILMLVYVTK FFWWEAGYWN
260 270 280 290 300
TMDIAHDRAG FYICWGCLVW VPSVYTSPGM YLVNHPVELG TQLAIYILVA
310 320 330 340 350
GILCIYINYD CDRQRQEFRR TNGKCLVWGR APSKIVASYT TTSGETKTSL
360 370 380 390 400
LLTSGWWGLA RHFHYVPEIL SAFFWTVPAL FDNFLAYFYV IFLTLLLFDR
410 420 430
AKRDDDRCRS KYGKYWKLYC EKVKYRIIPG IY
Length:432
Mass (Da):49,591
Last modified:October 1, 2000 - v1
Checksum:i5458E495BE1EF4B0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti117Y → H in AAC49278 (PubMed:8631902).Curated1
Sequence conflicti308N → K in AAC49278 (PubMed:8631902).Curated1
Sequence conflicti391 – 392Missing in AAC49278 (PubMed:8631902).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U49398 mRNA Translation: AAC49278.1
AF239701 Genomic DNA Translation: AAF63498.1
AC012561 Genomic DNA Translation: AAF87888.1
CP002684 Genomic DNA Translation: AEE32547.1
AY099589 mRNA Translation: AAM20440.1
BT000245 mRNA Translation: AAN15564.1
PIRiF96540
RefSeqiNP_175460.1, NM_103926.5 [Q9LDU6-1]
UniGeneiAt.25165

Genome annotation databases

EnsemblPlantsiAT1G50430.1; AT1G50430.1; AT1G50430 [Q9LDU6-1]
GeneIDi841465
GrameneiAT1G50430.1; AT1G50430.1; AT1G50430 [Q9LDU6-1]
KEGGiath:AT1G50430

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiST7R_ARATH
AccessioniPrimary (citable) accession number: Q9LDU6
Secondary accession number(s): Q38930
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 31, 2002
Last sequence update: October 1, 2000
Last modified: April 25, 2018
This is version 132 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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