Reviewed, UniProtKB/Swiss-Prot Q9LDR4 (ERG24_ARATH)

Names and origin

Protein names

Recommended name:
    Delta(14)-sterol reductase
    EC=1.3.1.70
Alternative name(s):
    C-14 sterol reductase
    Sterol C14-reductase
    Protein FACKEL

Gene names

Name:	FK
Ordered Locus Names:	At3g52940
ORF Names:	F8J2_111

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	369 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Reduces the C14=C15 double bond of 4,4-dimethyl-cholesta-8,14,24-trienol to produce 4,4-dimethyl-cholesta-8,24-dienol. Required for cell division and expansion and is involved in proper organization of the embryo.
Catalytic activity	4,4-dimethyl-5-alpha-cholesta-8,24-dien-3-beta-ol + NADP+ = 4,4-dimethyl-5-alpha-cholesta-8,14,24-trien-3-beta-ol + NADPH.
Pathway	Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 2/6.
Subcellular location	Membrane; Multi-pass membrane protein Probable.
Sequence similarities	Belongs to the ERG4/ERG24 family.

Ontologies

Keywords
Biological process	Lipid synthesis Steroid biosynthesis Sterol biosynthesis
Cellular component	Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW sterol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	delta14-sterol reductase activity Ref.1 Ref.2 Inferred from mutant phenotype. Source: TAIR
Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q9LDR4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 369	369	Delta(14)-sterol reductase		PRO_0000207499
Regions
	Transmembrane	15 – 34	20	Potential
	Transmembrane	54 – 76	23	Potential
	Transmembrane	86 – 105	20	Potential
	Transmembrane	146 – 168	23	Potential
	Transmembrane	178 – 195	18	Potential
	Transmembrane	208 – 230	23	Potential
	Transmembrane	240 – 262	23	Potential
	Transmembrane	275 – 297	23	Potential
	Transmembrane	317 – 336	20	Potential

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified January 31, 2002. Version 2. Checksum: F18674BCF27C0502	FASTA	369	41,913
	10 20 30 40 50 60 MLLDMDLGVL LPSLQSVYVL VFYFVYLAVA GEILPGKVIR GVLLSDGSQL RYRCNGLLAL 70 80 90 100 110 120 ILLVAILGIC AKLGIVSPLV VADRGLELLS ATFIFCVLVT LALYVTGRSS SNKGSSLKPH 130 140 150 160 170 180 VSGNLVHDWW FGIQLNPQFM SIDLKFFFVR AGMMGWLLIN LSILAKSVQD GSLSQSMILY 190 200 210 220 230 240 QIFCALYILD YFVHEEYMTS TWDIIAERLG FMLVFGDLLW IPFTFSIQGW WLLHNKVELT 250 260 270 280 290 300 VPAIVVNCLV FLIGYMVFRG ANKQKHIFKK NPKTPIWGKP PVVVGGKLLV SGYWGIARHC 310 320 330 340 350 360 NYLGDLMLAL SFSLPCGISS PVPYFYPIYL LILLIWRERR DEVRCAEKYK EIWAEYLRLV PWRILPYVY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"FACKEL is a sterol C-14 reductase required for organized cell division and expansion in Arabidopsis embryogenesis." Schrick K., Mayer U., Horrichs A., Kuhnt C., Bellini C., Dangl J., Schmidt J., Juergens G. Genes Dev. 14:1471-1484(2000) [PubMed: 10859166] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION.
[2]	"A critical role of sterols in embryonic patterning and meristem programming revealed by the fackel mutants of Arabidopsis thaliana." Jang J.-C., Fujioka S., Tasaka M., Seto H., Takatsuto S., Ishii A., Aida M., Yoshida S., Sheen J. Genes Dev. 14:1485-1497(2000) [PubMed: 10859167] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[3]	"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana." Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. Nature 408:820-822(2000) [PubMed: 11130713] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF256535 mRNA. Translation: AAF82282.1. AF256536 Genomic DNA. Translation: AAF82283.1. AF257178 mRNA. Translation: AAF81279.1. Different initiation. AF263244 Genomic DNA. Translation: AAF82768.1. AL132969 Genomic DNA. Translation: CAC01296.1. Different initiation. AY064964 mRNA. Translation: AAL38381.1. AY133650 mRNA. Translation: AAM91480.1.
IPI	IPI00539254.
RefSeq	NP_566975.1.
UniGene	At.9856
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q9LDR4.
Proteomic databases
PRIDE	Q9LDR4.
Genome annotation databases
GeneID	824460.
GenomeReviews	Gene locus AT3G52940 in contig BA000014_GR.
KEGG	ath:AT3G52940.
NMPDR	fig|3702.1.peg.16568.
Organism-specific databases
TAIR	At3g52940.
Phylogenomic databases
OMA	IQGWWLL.
Enzyme and pathway databases
BioCyc	MetaCyc:AT3G52940-MON.
BRENDA	1.3.1.70. 302.
Gene expression databases
ArrayExpress	Q9LDR4.
Genevestigator	Q9LDR4.
Family and domain databases
InterPro	IPR001171. Ergosterol_biosynth_ERG4_ERG24. IPR018083. Sterol_reductase_CS. [Graphical view]
Pfam	PF01222. ERG4_ERG24. 1 hit. [Graphical view]
PROSITE	PS01017. STEROL_REDUCT_1. 1 hit. PS01018. STEROL_REDUCT_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ERG24_ARATH

Accession

Primary (citable) accession number: Q9LDR4
Secondary accession number(s): Q9LD62

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 31, 2002
Last sequence update:	January 31, 2002
Last modified:	November 3, 2009
This is version 60 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)