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Protein

CBL-interacting serine/threonine-protein kinase 24

Gene

CIPK24

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the regulatory pathway for the control of intracellular Na+ and K+ homeostasis and salt tolerance. Activates the vacuolar H+/Ca2+ antiporter CAX1 and operates in synergy with CBL4/SOS3 to activate the plasma membrane Na+/H+ antiporter SOS1. CIPK serine-threonine protein kinases interact with CBL proteins. Binding of a CBL protein to the regulatory NAF domain of CIPK protein lead to the activation of the kinase in a calcium-dependent manner. Phosphorylates CBL1, CBL4 and CBL10.4 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.1 Publication

Cofactori

Mn2+1 Publication

Kineticsi

  1. KM=131.2 µM for synthetic substrate1 Publication
  1. Vmax=1304 pmol/min/mg enzyme1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei40ATPPROSITE-ProRule annotation1
Active sitei134Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi17 – 25ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • protein kinase activity Source: TAIR
  • protein serine/threonine kinase activity Source: GO_Central

GO - Biological processi

  • intracellular signal transduction Source: GO_Central
  • response to salt stress Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Manganese, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
CBL-interacting serine/threonine-protein kinase 24 (EC:2.7.11.1)
Alternative name(s):
Protein SALT OVERLY SENSITIVE 2
SNF1-related kinase 3.11
Gene namesi
Name:CIPK24
Synonyms:SnRK3.11, SOS2
Ordered Locus Names:At5g35410
ORF Names:F6I13.1, K21B8.3
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G35410.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: GO_Central
  • plant-type vacuole membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi40K → N: Abolishes autophosphorylation. 1 Publication1
Mutagenesisi156S → D: Increases kinase activity. 1 Publication1
Mutagenesisi168T → D: Increases kinase activity. 1 Publication1
Mutagenesisi175Y → D: Increases kinase activity. 1 Publication1
Mutagenesisi197G → E: Abolishes autophosphorylation. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000858771 – 446CBL-interacting serine/threonine-protein kinase 24Add BLAST446

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei156PhosphoserineBy similarity1
Modified residuei168PhosphothreonineBy similarity1

Post-translational modificationi

Autophosphorylated.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9LDI3.
PRIDEiQ9LDI3.

PTM databases

iPTMnetiQ9LDI3.

Expressioni

Inductioni

Up-regulated in roots by salt stress.

Gene expression databases

ExpressionAtlasiQ9LDI3. baseline and differential.
GenevisibleiQ9LDI3. AT.

Interactioni

Subunit structurei

Interacts with CBL1, CBL2, CBL4/SOS3, CBL5, CBL9, CBL10 and with the protein phosphatase 2C ABI2.6 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ABI2O047194EBI-537551,EBI-537680
CAM4P258542EBI-537551,EBI-1235664
CAM7P592202EBI-537551,EBI-1236031
CBL1O814453EBI-537551,EBI-974530
CBL10Q7FRS85EBI-537551,EBI-2026616
CBL10Q7FRS8-24EBI-537551,EBI-2026677
CBL4O812239EBI-537551,EBI-537541
CML9Q9S7442EBI-537551,EBI-1236048
NDPK2O649033EBI-537551,EBI-349517

Protein-protein interaction databases

BioGridi18756. 34 interactors.
DIPiDIP-34745N.
IntActiQ9LDI3. 30 interactors.
MINTiMINT-274819.
STRINGi3702.AT5G35410.1.

Structurei

Secondary structure

1446
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi11 – 13Combined sources3
Beta strandi24 – 30Combined sources7
Turni31 – 34Combined sources4
Beta strandi35 – 43Combined sources9
Helixi44 – 48Combined sources5
Helixi53 – 65Combined sources13
Beta strandi77 – 79Combined sources3
Beta strandi81 – 88Combined sources8
Beta strandi92 – 95Combined sources4
Helixi97 – 103Combined sources7
Helixi108 – 127Combined sources20
Turni137 – 139Combined sources3
Beta strandi140 – 142Combined sources3
Beta strandi148 – 150Combined sources3
Beta strandi173 – 176Combined sources4
Helixi178 – 182Combined sources5
Helixi188 – 205Combined sources18
Beta strandi212 – 214Combined sources3
Helixi215 – 224Combined sources10
Helixi235 – 244Combined sources10
Turni249 – 251Combined sources3
Helixi255 – 260Combined sources6
Helixi262 – 265Combined sources4
Helixi281 – 286Combined sources6
Helixi312 – 316Combined sources5
Turni320 – 322Combined sources3
Helixi324 – 328Combined sources5
Beta strandi340 – 343Combined sources4
Helixi347 – 360Combined sources14
Beta strandi363 – 376Combined sources14
Helixi381 – 383Combined sources3
Beta strandi386 – 395Combined sources10
Beta strandi398 – 408Combined sources11
Helixi410 – 423Combined sources14
Turni425 – 427Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2EHBX-ray2.10D304-446[»]
4D28X-ray3.30A/B/C/D1-446[»]
ProteinModelPortaliQ9LDI3.
SMRiQ9LDI3.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9LDI3.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini11 – 264Protein kinasePROSITE-ProRule annotationAdd BLAST254
Domaini305 – 329NAFPROSITE-ProRule annotationAdd BLAST25

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni152 – 179Activation loopAdd BLAST28
Regioni336 – 365PPIAdd BLAST30

Domaini

The activation loop within the kinase domain is the target of phosphorylation/activation by upstream protein kinases. The PPI motif mediates the interaction with the ABI (abscisic acid-insensitive) phosphatases. The C-terminal part (309-446) of the protein is required for the phosphorylation of CBL, but is not involved in autophosphorylation.1 Publication

Sequence similaritiesi

Contains 1 NAF domain.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0583. Eukaryota.
COG0515. LUCA.
HOGENOMiHOG000233016.
InParanoidiQ9LDI3.
OMAiWFSPGAT.
OrthoDBiEOG093609SU.
PhylomeDBiQ9LDI3.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR018451. NAF/FISL_domain.
IPR004041. NAF_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF03822. NAF. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50816. NAF. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9LDI3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKKMRRVGK YEVGRTIGEG TFAKVKFARN TDTGDNVAIK IMAKSTILKN
60 70 80 90 100
RMVDQIKREI SIMKIVRHPN IVRLYEVLAS PSKIYIVLEF VTGGELFDRI
110 120 130 140 150
VHKGRLEESE SRKYFQQLVD AVAHCHCKGV YHRDLKPENL LLDTNGNLKV
160 170 180 190 200
SDFGLSALPQ EGVELLRTTC GTPNYVAPEV LSGQGYDGSA ADIWSCGVIL
210 220 230 240 250
FVILAGYLPF SETDLPGLYR KINAAEFSCP PWFSAEVKFL IHRILDPNPK
260 270 280 290 300
TRIQIQGIKK DPWFRLNYVP IRAREEEEVN LDDIRAVFDG IEGSYVAENV
310 320 330 340 350
ERNDEGPLMM NAFEMITLSQ GLNLSALFDR RQDFVKRQTR FVSRREPSEI
360 370 380 390 400
IANIEAVANS MGFKSHTRNF KTRLEGLSSI KAGQLAVVIE IYEVAPSLFM
410 420 430 440
VDVRKAAGET LEYHKFYKKL CSKLENIIWR ATEGIPKSEI LRTITF
Length:446
Mass (Da):50,635
Last modified:October 1, 2000 - v1
Checksum:i99FDF3A778E1093D
GO

Sequence cautioni

The sequence AAF67384 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF237670 Genomic DNA. Translation: AAF62923.1.
AF395081 mRNA. Translation: AAK72257.1.
AB025611 Genomic DNA. Translation: BAA98146.1.
AF262044 Genomic DNA. Translation: AAF67384.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED93966.1.
AY099621 mRNA. Translation: AAM20472.1.
BT002138 mRNA. Translation: AAN72149.1.
RefSeqiNP_198391.1. NM_122932.5.
UniGeneiAt.7930.

Genome annotation databases

EnsemblPlantsiAT5G35410.1; AT5G35410.1; AT5G35410.
GeneIDi833502.
GrameneiAT5G35410.1; AT5G35410.1; AT5G35410.
KEGGiath:AT5G35410.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF237670 Genomic DNA. Translation: AAF62923.1.
AF395081 mRNA. Translation: AAK72257.1.
AB025611 Genomic DNA. Translation: BAA98146.1.
AF262044 Genomic DNA. Translation: AAF67384.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED93966.1.
AY099621 mRNA. Translation: AAM20472.1.
BT002138 mRNA. Translation: AAN72149.1.
RefSeqiNP_198391.1. NM_122932.5.
UniGeneiAt.7930.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2EHBX-ray2.10D304-446[»]
4D28X-ray3.30A/B/C/D1-446[»]
ProteinModelPortaliQ9LDI3.
SMRiQ9LDI3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi18756. 34 interactors.
DIPiDIP-34745N.
IntActiQ9LDI3. 30 interactors.
MINTiMINT-274819.
STRINGi3702.AT5G35410.1.

PTM databases

iPTMnetiQ9LDI3.

Proteomic databases

PaxDbiQ9LDI3.
PRIDEiQ9LDI3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G35410.1; AT5G35410.1; AT5G35410.
GeneIDi833502.
GrameneiAT5G35410.1; AT5G35410.1; AT5G35410.
KEGGiath:AT5G35410.

Organism-specific databases

TAIRiAT5G35410.

Phylogenomic databases

eggNOGiKOG0583. Eukaryota.
COG0515. LUCA.
HOGENOMiHOG000233016.
InParanoidiQ9LDI3.
OMAiWFSPGAT.
OrthoDBiEOG093609SU.
PhylomeDBiQ9LDI3.

Miscellaneous databases

EvolutionaryTraceiQ9LDI3.
PROiQ9LDI3.

Gene expression databases

ExpressionAtlasiQ9LDI3. baseline and differential.
GenevisibleiQ9LDI3. AT.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR018451. NAF/FISL_domain.
IPR004041. NAF_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF03822. NAF. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50816. NAF. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCIPKO_ARATH
AccessioniPrimary (citable) accession number: Q9LDI3
Secondary accession number(s): Q9LKR2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 21, 2004
Last sequence update: October 1, 2000
Last modified: November 30, 2016
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.