Q9LDH0 (XYLT_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-(1,2)-xylosyltransferase
EC=2.4.2.38

Gene names

Name:	XYLT
Ordered Locus Names:	At5g55500
ORF Names:	MTE17.21

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	534 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes beta-1,2 glycosidic linkages. Ref.1
Catalytic activity	UDP-D-xylose + N(4)-(N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-(N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6))-beta-D-mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl)asparagine = UDP + N(4)-(N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-(N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6))-(beta-D-xylosyl-(1->2))-beta-D-mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl)asparagine. Ref.1
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus › Golgi stack membrane; Single-pass type II membrane protein By similarity. Note: Membrane-bound form in trans cisternae of Golgi By similarity.
Allergenic properties	Renders plant glycoproteins immunogenic and allergenic in human. This is due to the presence of beta-1,2-xylose and/or core alpha-1,3-fucose which are not found in mammalian proteins, and which constitute epitopes for carbohydrate-reactive antibodies.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	posttranslational protein targeting to membrane, translocation Inferred from direct assay. Source: TAIR protein N-linked glycosylation Inferred from direct assay Ref.1. Source: TAIR
Cellular component	Golgi cisterna membrane Inferred from electronic annotation. Source: UniProtKB-SubCell Golgi medial cisterna Inferred from direct assay. Source: TAIR integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	glycoprotein 2-beta-D-xylosyltransferase activity Inferred from direct assay Ref.1. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 534	534	Beta-(1,2)-xylosyltransferase		PRO_0000080576
Regions
	Topological domain	1 – 11	11	Cytoplasmic Potential
	Transmembrane	12 – 34	23	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	35 – 534	500	Lumenal Potential
	Compositional bias	378 – 385	8	Poly-Ser
Amino acid modifications
	Glycosylation	51	1	N-linked (GlcNAc...) Potential
	Glycosylation	301	1	N-linked (GlcNAc...) Potential
	Glycosylation	479	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	54	1	A → S in AAF77064. Ref.3
	Sequence conflict	125	1	N → S in AAF77064. Ref.3
	Sequence conflict	383	1	Missing in AAF77064. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q9LDH0 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: A7DCA7FDE8365874	FASTA	534	60,235
	10 20 30 40 50 60 MSKRNPKILK IFLYMLLLNS LFLIIYFVFH SSSFSPEQSQ PPHIYHVSVN NQSAIQKPWP 70 80 90 100 110 120 ILPSYLPWTP PQRNLPTGSC EGYFGNGFTK RVDFLKPRIG GGGEGSWFRC FYSETLQSSI 130 140 150 160 170 180 CEGRNLRMVP DRIVMSRGGE KLEEVMGRKE EEELPAFRQG AFEVAEEVSS RLGFKRHRRF 190 200 210 220 230 240 GGGEGGSAVS RRLVNDEMLN EYMQEGGIDR HTMRDLVASI RAVDTNDFVC EEWVEEPTLL 250 260 270 280 290 300 VTRFEYANLF HTVTDWYSAY VSSRVTGLPN RPHVVFVDGH CTTQLEETWT ALFSGIRYAK 310 320 330 340 350 360 NFTKPVCFRH AILSPLGYET ALFKGLSGEI DCKGDSAHNL WQNPDDKRTA RISEFGEMIR 370 380 390 400 410 420 AAFGLPVNRH RSLEKPLSSS SSSASVYNVL FVRREDYLAH PRHGGKVQSR LINEEEVFDS 430 440 450 460 470 480 LHHWVATGST GLTKCGINLV NGLLAHMSMK DQVRAIQDAS VIIGAHGAGL THIVSATPNT 490 500 510 520 530 TIFEIISVEF QRPHFELIAK WKGLEYHAMH LANSRAEPTA VIEKLTEIMK SLGC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and functional expression of beta 1,2-xylosyltransferase cDNA from Arabidopsis thaliana." Strasser R., Mucha J., Mach L., Altmann F., Wilson I.B.H., Gloessl J., Steinkellner H. FEBS Lett. 472:105-108(2000) [PubMed: 10781814] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY. Strain: cv. Columbia.
[2]	"Arabidopsis cDNA for beta1,2-xylosyltransferase." Bakker H. Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	Gomord V., Baltresca V., Kiefer-Meyer M.-C., Faye L. Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Wassilewskija.
[4]	"Structural analysis of Arabidopsis thaliana chromosome 5. VII. Sequence features of the regions of 1,013,767 bp covered by sixteen physically assigned P1 and TAC clones." Nakamura Y., Sato S., Asamizu E., Kaneko T., Kotani H., Miyajima N., Tabata S. DNA Res. 5:297-308(1998) [PubMed: 9872454] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[5]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[6]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ272121 mRNA. Translation: CAB90610.1. AJ277603 mRNA. Translation: CAB89489.1. AF272852 mRNA. Translation: AAF77064.1. AB015479 Genomic DNA. Translation: BAB08567.1. CP002688 Genomic DNA. Translation: AED96635.1. AF411785 mRNA. Translation: AAL06475.1. AY143907 mRNA. Translation: AAN28846.1.
IPI	IPI00521380.
PIR	T52649.
RefSeq	NP_568825.1. NM_124932.3.
UniGene	At.1454.
3D structure databases
ProteinModelPortal	Q9LDH0.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9LDH0.
Protein family/group databases
CAZy	GT61. Glycosyltransferase Family 61.
Proteomic databases
PRIDE	Q9LDH0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G55500.1; AT5G55500.1; AT5G55500.
GeneID	835643.
GenomeReviews	Gene locus AT5G55500 in contig BA000015_GR.
KEGG	ath:AT5G55500.
NMPDR	fig|3702.1.peg.27471.
Organism-specific databases
TAIR	At5g55500.
Phylogenomic databases
eggNOG	KOG4698.
GeneTree	EPGT00050000013514.
HOGENOM	HBG746855.
InParanoid	Q9LDH0.
OMA	YANLFHT.
PhylomeDB	Q9LDH0.
ProtClustDB	CLSN2690024.
Enzyme and pathway databases
BioCyc	ARA:AT5G55500-MONOMER. MetaCyc:AT5G55500-MONOMER.
BRENDA	2.4.2.38. 399.
Gene expression databases
ArrayExpress	Q9LDH0.
Genevestigator	Q9LDH0.
GermOnline	AT5G55500. Arabidopsis thaliana.
Family and domain databases
KO	K03714.
ProtoNet	Search...

Entry information

Entry name

XYLT_ARATH

Accession

Primary (citable) accession number: Q9LDH0
Secondary accession number(s): Q9LKK7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 16, 2001
Last sequence update:	October 1, 2000
Last modified:	December 14, 2011
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program