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Q9LDH0 (XYLT_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Beta-(1,2)-xylosyltransferase

EC=2.4.2.38
Gene names
Name:XYLT
Ordered Locus Names:At5g55500
ORF Names:MTE17.21
OrganismArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonscore eudicotyledonsrosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length534 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes beta-1,2 glycosidic linkages. Ref.1

Catalytic activity

UDP-D-xylose + N(4)-(N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-(N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6))-beta-D-mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl)asparagine = UDP + N(4)-(N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-(N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6))-(beta-D-xylosyl-(1->2))-beta-D-mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl)asparagine. Ref.1

Pathway

Protein modification; protein glycosylation.

Subcellular location

Golgi apparatusGolgi stack membrane; Single-pass type II membrane protein By similarity. Note: Membrane-bound form in trans cisternae of Golgi By similarity.

Allergenic properties

Renders plant glycoproteins immunogenic and allergenic in human. This is due to the presence of beta-1,2-xylose and/or core alpha-1,3-fucose which are not found in mammalian proteins, and which constitute epitopes for carbohydrate-reactive antibodies.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 534534Beta-(1,2)-xylosyltransferase
PRO_0000080576

Regions

Topological domain1 – 1111Cytoplasmic Potential
Transmembrane12 – 3423Helical; Signal-anchor for type II membrane protein; Potential
Topological domain35 – 534500Lumenal Potential
Compositional bias378 – 3858Poly-Ser

Amino acid modifications

Glycosylation511N-linked (GlcNAc...) Potential
Glycosylation3011N-linked (GlcNAc...) Potential
Glycosylation4791N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict541A → S in AAF77064. Ref.3
Sequence conflict1251N → S in AAF77064. Ref.3
Sequence conflict3831Missing in AAF77064. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Q9LDH0 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: A7DCA7FDE8365874

FASTA53460,235
        10         20         30         40         50         60 
MSKRNPKILK IFLYMLLLNS LFLIIYFVFH SSSFSPEQSQ PPHIYHVSVN NQSAIQKPWP 

        70         80         90        100        110        120 
ILPSYLPWTP PQRNLPTGSC EGYFGNGFTK RVDFLKPRIG GGGEGSWFRC FYSETLQSSI 

       130        140        150        160        170        180 
CEGRNLRMVP DRIVMSRGGE KLEEVMGRKE EEELPAFRQG AFEVAEEVSS RLGFKRHRRF 

       190        200        210        220        230        240 
GGGEGGSAVS RRLVNDEMLN EYMQEGGIDR HTMRDLVASI RAVDTNDFVC EEWVEEPTLL 

       250        260        270        280        290        300 
VTRFEYANLF HTVTDWYSAY VSSRVTGLPN RPHVVFVDGH CTTQLEETWT ALFSGIRYAK 

       310        320        330        340        350        360 
NFTKPVCFRH AILSPLGYET ALFKGLSGEI DCKGDSAHNL WQNPDDKRTA RISEFGEMIR 

       370        380        390        400        410        420 
AAFGLPVNRH RSLEKPLSSS SSSASVYNVL FVRREDYLAH PRHGGKVQSR LINEEEVFDS 

       430        440        450        460        470        480 
LHHWVATGST GLTKCGINLV NGLLAHMSMK DQVRAIQDAS VIIGAHGAGL THIVSATPNT 

       490        500        510        520        530 
TIFEIISVEF QRPHFELIAK WKGLEYHAMH LANSRAEPTA VIEKLTEIMK SLGC 

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning and functional expression of beta 1,2-xylosyltransferase cDNA from Arabidopsis thaliana."
Strasser R., Mucha J., Mach L., Altmann F., Wilson I.B.H., Gloessl J., Steinkellner H.
FEBS Lett. 472:105-108(2000) [PubMed: 10781814] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY.
Strain: cv. Columbia.
[2]"Arabidopsis cDNA for beta1,2-xylosyltransferase."
Bakker H.
Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]Gomord V., Baltresca V., Kiefer-Meyer M.-C., Faye L.
Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Wassilewskija.
[4]"Structural analysis of Arabidopsis thaliana chromosome 5. VII. Sequence features of the regions of 1,013,767 bp covered by sixteen physically assigned P1 and TAC clones."
Nakamura Y., Sato S., Asamizu E., Kaneko T., Kotani H., Miyajima N., Tabata S.
DNA Res. 5:297-308(1998) [PubMed: 9872454] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[5]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[6]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed: 14593172] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ272121 mRNA. Translation: CAB90610.1.
AJ277603 mRNA. Translation: CAB89489.1.
AF272852 mRNA. Translation: AAF77064.1.
AB015479 Genomic DNA. Translation: BAB08567.1.
CP002688 Genomic DNA. Translation: AED96635.1.
AF411785 mRNA. Translation: AAL06475.1.
AY143907 mRNA. Translation: AAN28846.1.
IPIIPI00521380.
PIRT52649.
RefSeqNP_568825.1. NM_124932.3.
UniGeneAt.1454.

3D structure databases

ProteinModelPortalQ9LDH0.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ9LDH0.

Protein family/group databases

CAZyGT61. Glycosyltransferase Family 61.

Proteomic databases

PRIDEQ9LDH0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G55500.1; AT5G55500.1; AT5G55500.
GeneID835643.
GenomeReviewsGene locus AT5G55500 in contig BA000015_GR.
KEGGath:AT5G55500.
NMPDRfig|3702.1.peg.27471.

Organism-specific databases

TAIRAt5g55500.

Phylogenomic databases

eggNOGKOG4698.
GeneTreeEPGT00050000013514.
HOGENOMHBG746855.
InParanoidQ9LDH0.
OMAYANLFHT.
PhylomeDBQ9LDH0.
ProtClustDBCLSN2690024.

Enzyme and pathway databases

BioCycARA:AT5G55500-MONOMER.
MetaCyc:AT5G55500-MONOMER.
BRENDA2.4.2.38. 399.

Gene expression databases

ArrayExpressQ9LDH0.
GenevestigatorQ9LDH0.
GermOnlineAT5G55500. Arabidopsis thaliana.

Family and domain databases

KOK03714.
ProtoNetSearch...

Entry information

Entry nameXYLT_ARATH
AccessionPrimary (citable) accession number: Q9LDH0
Secondary accession number(s): Q9LKK7
Entry history
Integrated into UniProtKB/Swiss-Prot: November 16, 2001
Last sequence update: October 1, 2000
Last modified: December 14, 2011
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways