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Protein

LAG1 longevity assurance homolog 1

Gene

LAG1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Mediates resistance to sphinganine-analog mycotoxins (SAMs) by restoring the sphingolipid biosynthesis. Could salvage the transport of GPI-anchored proteins from the endoplasmic reticulum to the Golgi apparatus in ceramides-depleted cells after SAM exposure (By similarity).By similarity

GO - Molecular functioni

  • sphingosine N-acyltransferase activity Source: TAIR

GO - Biological processi

  • ceramide biosynthetic process Source: GO_Central
  • very long-chain fatty acid biosynthetic process Source: TAIR
Complete GO annotation...

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism

Enzyme and pathway databases

ReactomeiR-ATH-1660661. Sphingolipid de novo biosynthesis.

Names & Taxonomyi

Protein namesi
Recommended name:
LAG1 longevity assurance homolog 1
Short name:
LAG1 homolog 1
Gene namesi
Name:LAG1
Ordered Locus Names:At3g25540
ORF Names:MWL2.19
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G25540.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei16 – 3621HelicalSequence analysisAdd
BLAST
Transmembranei85 – 10521HelicalSequence analysisAdd
BLAST
Transmembranei131 – 15121HelicalSequence analysisAdd
BLAST
Transmembranei157 – 17721HelicalSequence analysisAdd
BLAST
Transmembranei216 – 23621HelicalSequence analysisAdd
BLAST
Transmembranei260 – 28021HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • endoplasmic reticulum Source: TAIR
  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • Golgi apparatus Source: TAIR
  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 310310LAG1 longevity assurance homolog 1PRO_0000185518Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei300 – 3001PhosphoserineBy similarity
Modified residuei302 – 3021PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9LDF2.
PRIDEiQ9LDF2.

PTM databases

iPTMnetiQ9LDF2.

Expressioni

Gene expression databases

GenevisibleiQ9LDF2. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT3G25540.1.

Structurei

3D structure databases

ProteinModelPortaliQ9LDF2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini76 – 289214TLCPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 TLC (TRAM/LAG1/CLN8) domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1607. Eukaryota.
COG5058. LUCA.
HOGENOMiHOG000239939.
InParanoidiQ9LDF2.
OMAiHHITTLV.
PhylomeDBiQ9LDF2.

Family and domain databases

InterProiIPR016439. Lag1/Lac1.
IPR006634. TLC-dom.
[Graphical view]
PANTHERiPTHR12560. PTHR12560. 2 hits.
PfamiPF03798. TRAM_LAG1_CLN8. 1 hit.
[Graphical view]
PIRSFiPIRSF005225. LAG1_LAC1. 1 hit.
SMARTiSM00724. TLC. 1 hit.
[Graphical view]
PROSITEiPS50922. TLC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9LDF2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGLFESVKSI DWEQESFPTY QDLGFLPLFA VFFPTIRFLL DRFVFEKLAS
60 70 80 90 100
LVIYGRMSTN KSDNIKDRKK NSPKVRKFKE SAWKCIYYLS AELLALSVTY
110 120 130 140 150
NEPWFSNTLY FWIGPGDQIW PDQPMKMKLK FLYMFAAGFY TYSIFALVFW
160 170 180 190 200
ETRRSDFGVS MGHHITTLVL IVLSYICRLT RAGSVILALH DASDVFLEIG
210 220 230 240 250
KMSKYCGAES LASISFVLFA LSWVVLRLIY YPFWILWSTS YQIIMTVDKE
260 270 280 290 300
KHPNGPILYY MFNTLLYFLL VLHIFWWVLI YRMLVKQVQD RGKLSEDVRS
310
DSESDDEHED
Length:310
Mass (Da):36,498
Last modified:October 1, 2000 - v1
Checksum:iB3E1F2D5D3A4AEAD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF198179 mRNA. Translation: AAF66102.1.
AB025639 Genomic DNA. Translation: BAB01323.1.
CP002686 Genomic DNA. Translation: AEE77023.1.
AF360152 mRNA. Translation: AAK25862.1.
AY142597 mRNA. Translation: AAN13166.1.
RefSeqiNP_566769.1. NM_113450.5.
UniGeneiAt.6098.

Genome annotation databases

EnsemblPlantsiAT3G25540.1; AT3G25540.1; AT3G25540.
GeneIDi822140.
GrameneiAT3G25540.1; AT3G25540.1; AT3G25540.
KEGGiath:AT3G25540.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF198179 mRNA. Translation: AAF66102.1.
AB025639 Genomic DNA. Translation: BAB01323.1.
CP002686 Genomic DNA. Translation: AEE77023.1.
AF360152 mRNA. Translation: AAK25862.1.
AY142597 mRNA. Translation: AAN13166.1.
RefSeqiNP_566769.1. NM_113450.5.
UniGeneiAt.6098.

3D structure databases

ProteinModelPortaliQ9LDF2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT3G25540.1.

PTM databases

iPTMnetiQ9LDF2.

Proteomic databases

PaxDbiQ9LDF2.
PRIDEiQ9LDF2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G25540.1; AT3G25540.1; AT3G25540.
GeneIDi822140.
GrameneiAT3G25540.1; AT3G25540.1; AT3G25540.
KEGGiath:AT3G25540.

Organism-specific databases

TAIRiAT3G25540.

Phylogenomic databases

eggNOGiKOG1607. Eukaryota.
COG5058. LUCA.
HOGENOMiHOG000239939.
InParanoidiQ9LDF2.
OMAiHHITTLV.
PhylomeDBiQ9LDF2.

Enzyme and pathway databases

ReactomeiR-ATH-1660661. Sphingolipid de novo biosynthesis.

Miscellaneous databases

PROiQ9LDF2.

Gene expression databases

GenevisibleiQ9LDF2. AT.

Family and domain databases

InterProiIPR016439. Lag1/Lac1.
IPR006634. TLC-dom.
[Graphical view]
PANTHERiPTHR12560. PTHR12560. 2 hits.
PfamiPF03798. TRAM_LAG1_CLN8. 1 hit.
[Graphical view]
PIRSFiPIRSF005225. LAG1_LAC1. 1 hit.
SMARTiSM00724. TLC. 1 hit.
[Graphical view]
PROSITEiPS50922. TLC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A longevity assurance gene homolog of tomato mediates resistance to Alternaria alternata f. sp. lycopersici toxins and fumonisin B1."
    Brandwagt B.F., Mesbah L.A., Takken F.L.W., Laurent P.L., Kneppers T.J.A., Hille J., Nijkamp H.J.J.
    Proc. Natl. Acad. Sci. U.S.A. 97:4961-4966(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
  2. "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones."
    Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.
    DNA Res. 7:131-135(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.

Entry informationi

Entry nameiLAG11_ARATH
AccessioniPrimary (citable) accession number: Q9LDF2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: October 1, 2000
Last modified: February 17, 2016
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.