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Protein

Methylcrotonoyl-CoA carboxylase beta chain, mitochondrial

Gene

MCCB

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Carboxyltransferase subunit of the 3-methylcrotonyl-CoA carboxylase, an enzyme that catalyzes the conversion of 3-methylcrotonyl-CoA to 3-methylglutaconyl-CoA, a critical step for leucine and isovaleric acid catabolism.By similarity

Miscellaneous

Temporal and spatial accumulation of the alpha and beta subunits during development at approximately equal molar ratios.

Catalytic activityi

ATP + 3-methylcrotonoyl-CoA + HCO3- = ADP + phosphate + 3-methylglutaconyl-CoA.

Pathwayi: L-leucine degradation

This protein is involved in step 2 of the subpathway that synthesizes (S)-3-hydroxy-3-methylglutaryl-CoA from 3-isovaleryl-CoA.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Isovaleryl-CoA dehydrogenase, mitochondrial (IVD)
  2. Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial (MCCA), Methylcrotonoyl-CoA carboxylase beta chain, mitochondrial (MCCB)
  3. no protein annotated in this organism
This subpathway is part of the pathway L-leucine degradation, which is itself part of Amino-acid degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-3-hydroxy-3-methylglutaryl-CoA from 3-isovaleryl-CoA, the pathway L-leucine degradation and in Amino-acid degradation.

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • cobalt ion binding Source: TAIR
  • methylcrotonoyl-CoA carboxylase activity Source: UniProtKB-EC
  • zinc ion binding Source: TAIR

GO - Biological processi

  • leucine catabolic process Source: TAIR

Keywordsi

Molecular functionLigase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT4G34030-MONOMER
BRENDAi6.4.1.4 399
ReactomeiR-ATH-196780 Biotin transport and metabolism
R-ATH-70895 Branched-chain amino acid catabolism
UniPathwayiUPA00363; UER00861

Names & Taxonomyi

Protein namesi
Recommended name:
Methylcrotonoyl-CoA carboxylase beta chain, mitochondrial (EC:6.4.1.4)
Short name:
MCCase subunit beta
Alternative name(s):
3-methylcrotonyl-CoA carboxylase 2
3-methylcrotonyl-CoA:carbon dioxide ligase subunit beta
Gene namesi
Name:MCCB
Ordered Locus Names:At4g34030
ORF Names:F17I5.220, F28A23.210
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G34030
TAIRilocus:2124256 AT4G34030

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 26MitochondrionSequence analysisAdd BLAST26
ChainiPRO_000000029227 – 587Methylcrotonoyl-CoA carboxylase beta chain, mitochondrialAdd BLAST561

Proteomic databases

PaxDbiQ9LDD8
PRIDEiQ9LDD8

Expressioni

Tissue specificityi

In roots, cotyledons, leaves, flowers, ovaries, siliques and embryos.

Gene expression databases

ExpressionAtlasiQ9LDD8 baseline and differential
GenevisibleiQ9LDD8 AT

Interactioni

Subunit structurei

Probably a heterodimer composed of biotin-containing alpha subunits and beta subunits.By similarity

Protein-protein interaction databases

BioGridi14831, 2 interactors
IntActiQ9LDD8, 1 interactor
STRINGi3702.AT4G34030.1

Structurei

3D structure databases

ProteinModelPortaliQ9LDD8
SMRiQ9LDD8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini68 – 324CoA carboxyltransferase N-terminalPROSITE-ProRule annotationAdd BLAST257
Domaini333 – 579CoA carboxyltransferase C-terminalPROSITE-ProRule annotationAdd BLAST247

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni68 – 579CarboxyltransferasePROSITE-ProRule annotationAdd BLAST512
Regioni367 – 396Acyl-CoA bindingSequence analysisAdd BLAST30

Sequence similaritiesi

Belongs to the AccD/PCCB family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0540 Eukaryota
COG4799 LUCA
HOGENOMiHOG000218692
InParanoidiQ9LDD8
KOiK01969
OMAiMCGKAYD
OrthoDBiEOG093606CI
PhylomeDBiQ9LDD8

Family and domain databases

InterProiView protein in InterPro
IPR034733 AcCoA_carboxyl
IPR029045 ClpP/crotonase-like_dom_sf
IPR011763 COA_CT_C
IPR011762 COA_CT_N
PfamiView protein in Pfam
PF01039 Carboxyl_trans, 1 hit
SUPFAMiSSF52096 SSF52096, 2 hits
PROSITEiView protein in PROSITE
PS50989 COA_CT_CTER, 1 hit
PS50980 COA_CT_NTER, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9LDD8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRILGRRVV SASKELTSIQ QWRIRPGTDS RPDPFRTFRG LQKGFCVGIL
60 70 80 90 100
PDGVDRNSEA FSSNSIAMEG ILSELRSHIK KVLAGGGEEA VKRNRSRNKL
110 120 130 140 150
LPRERIDRLL DPGSSFLELS QLAGHELYEE PLPSGGIITG IGPIHGRICM
160 170 180 190 200
FMANDPTVKG GTYYPITIKK HLRAQEIAAR CRLPCIYLVD SGGAYLPKQA
210 220 230 240 250
EVFPDKENFG RVFYNESVMS SDGIPQIAIV LGSCTAGGAY IPAMADESVM
260 270 280 290 300
VKGNGTIFLA GPPLVKAATG EEVSAEDLGG ATVHCTVSGV SDYFAQDELH
310 320 330 340 350
GLAIGRNIVK NLHMAAKQGM EGTFGSKNLV YKEPLYDINE LRSIAPVDHK
360 370 380 390 400
QQFDVRSIIA RIVDGSEFDE FKKQYGTTLV TGFARIYGQT VGIIGNNGIL
410 420 430 440 450
FNESALKGAH FIELCSQRKI PLVFLQNITG FMVGSRAEAN GIAKAGAKMV
460 470 480 490 500
MAVSCAKVPK ITIITGASFG AGNYAMCGRA YSPDFMFIWP NARIGIMGGA
510 520 530 540 550
QAAGVLTQIE RATKKRQGIK WTEEEEEAFK KKTVDAYERE ANPYYSTARL
560 570 580
WDDGVIDPCD TRKVLGLCLS AALNRPLEDT RFGVFRM
Length:587
Mass (Da):64,013
Last modified:October 1, 2000 - v1
Checksum:iBD6E0EEFDC762955
GO

Sequence cautioni

The sequence CAA17569 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAA19885 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAB80120 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti143P → T in AAK62371 (PubMed:14593172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF059511 Genomic DNA Translation: AAF35259.1
AF059510 mRNA Translation: AAF35258.1
AL021961 Genomic DNA Translation: CAA17569.1 Sequence problems.
AL031032 Genomic DNA Translation: CAA19885.1 Sequence problems.
AL161584 Genomic DNA Translation: CAB80120.1 Sequence problems.
CP002687 Genomic DNA Translation: AEE86312.1
AF386926 mRNA Translation: AAK62371.1
BT000384 mRNA Translation: AAN15703.1
RefSeqiNP_567950.1, NM_119564.5
UniGeneiAt.19955

Genome annotation databases

EnsemblPlantsiAT4G34030.1; AT4G34030.1; AT4G34030
GeneIDi829549
GrameneiAT4G34030.1; AT4G34030.1; AT4G34030
KEGGiath:AT4G34030

Similar proteinsi

Entry informationi

Entry nameiMCCB_ARATH
AccessioniPrimary (citable) accession number: Q9LDD8
Secondary accession number(s): O49501, Q94F35
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 2, 2002
Last sequence update: October 1, 2000
Last modified: May 23, 2018
This is version 130 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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