Reviewed, UniProtKB/Swiss-Prot Q9LDD8 (MCCB_ARATH)

Names and origin

Protein names

Recommended name:
    Methylcrotonoyl-CoA carboxylase beta chain, mitochondrial
      Short name=MCCase subunit beta
    EC=6.4.1.4
Alternative name(s):
    3-methylcrotonyl-CoA:carbon dioxide ligase subunit beta
    3-methylcrotonyl-CoA carboxylase 2

Gene names

Name:	MCCB
Ordered Locus Names:	At4g34030
ORF Names:	F28A23.210, F17I5.220

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	587 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + 3-methylcrotonoyl-CoA + HCO3- = ADP + phosphate + 3-methylglutaconyl-CoA.
Pathway	Amino-acid degradation; L-leucine degradation; (S)-3-hydroxy-3-methylglutaryl-CoA from 3-isovaleryl-CoA: step 2/3.
Subunit structure	Probably a heterodimer composed of biotin-containing alpha subunits and beta subunits By similarity.
Subcellular location	Mitochondrion matrix.
Tissue specificity	In roots, cotyledons, leaves, flowers, ovaries, siliques and embryos.
Miscellaneous	Temporal and spatial accumulation of the alpha and beta subunits during development at approximately equal molar ratios.
Sequence similarities	Belongs to the accD/PCCB family. Contains 1 carboxyltransferase domain.
Sequence caution	The sequence CAA17569.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAA19885.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAB80120.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	leucine catabolic process Inferred from direct assay. Source: TAIR
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW methylcrotonoyl-CoA carboxylase activity Inferred from direct assay. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 26	26	Mitochondrion Potential
	Chain	27 – 587	561	Methylcrotonoyl-CoA carboxylase beta chain, mitochondrial		PRO_0000000292
Regions
	Domain	74 – 581	508	Carboxyltransferase
	Region	367 – 396	30	Acyl-CoA binding Potential
Experimental info
	Sequence conflict	143	1	P → T in AAK62371. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q9LDD8-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: BD6E0EEFDC762955	FASTA	587	64,013
	10 20 30 40 50 60 MLRILGRRVV SASKELTSIQ QWRIRPGTDS RPDPFRTFRG LQKGFCVGIL PDGVDRNSEA 70 80 90 100 110 120 FSSNSIAMEG ILSELRSHIK KVLAGGGEEA VKRNRSRNKL LPRERIDRLL DPGSSFLELS 130 140 150 160 170 180 QLAGHELYEE PLPSGGIITG IGPIHGRICM FMANDPTVKG GTYYPITIKK HLRAQEIAAR 190 200 210 220 230 240 CRLPCIYLVD SGGAYLPKQA EVFPDKENFG RVFYNESVMS SDGIPQIAIV LGSCTAGGAY 250 260 270 280 290 300 IPAMADESVM VKGNGTIFLA GPPLVKAATG EEVSAEDLGG ATVHCTVSGV SDYFAQDELH 310 320 330 340 350 360 GLAIGRNIVK NLHMAAKQGM EGTFGSKNLV YKEPLYDINE LRSIAPVDHK QQFDVRSIIA 370 380 390 400 410 420 RIVDGSEFDE FKKQYGTTLV TGFARIYGQT VGIIGNNGIL FNESALKGAH FIELCSQRKI 430 440 450 460 470 480 PLVFLQNITG FMVGSRAEAN GIAKAGAKMV MAVSCAKVPK ITIITGASFG AGNYAMCGRA 490 500 510 520 530 540 YSPDFMFIWP NARIGIMGGA QAAGVLTQIE RATKKRQGIK WTEEEEEAFK KKTVDAYERE 550 560 570 580 ANPYYSTARL WDDGVIDPCD TRKVLGLCLS AALNRPLEDT RFGVFRM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular characterization of the non-biotin-containing subunit of 3-methylcrotonyl-CoA carboxylase." McKean A.L., Ke J., Song J., Che P., Achenbach S., Nikolau B.J., Wurtele E.S. J. Biol. Chem. 275:5582-5590(2000) [PubMed: 10681539] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. Strain: cv. Columbia and cv. Landsberg erecta.
[2]	"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana." Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R. Nature 402:769-777(1999) [PubMed: 10617198] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF059511 Genomic DNA. Translation: AAF35259.1. AF059510 mRNA. Translation: AAF35258.1. AL021961 Genomic DNA. Translation: CAA17569.1. Sequence problems. AL031032 Genomic DNA. Translation: CAA19885.1. Sequence problems. AL161584 Genomic DNA. Translation: CAB80120.1. Sequence problems. AF386926 mRNA. Translation: AAK62371.1. BT000384 mRNA. Translation: AAN15703.1.
IPI	IPI00531611.
RefSeq	NP_567950.1.
UniGene	At.19955
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q9LDD8.
Proteomic databases
PRIDE	Q9LDD8.
Genome annotation databases
GeneID	829549.
GenomeReviews	Gene locus AT4G34030 in contig CT486007_GR.
KEGG	ath:AT4G34030.
NMPDR	fig|3702.1.peg.21458.
Organism-specific databases
TAIR	At4g34030.
Phylogenomic databases
OMA	VADHYAD.
Gene expression databases
ArrayExpress	Q9LDD8.
Genevestigator	Q9LDD8.
GermOnline	AT4G34030. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR000022. Carboxyl_trans. IPR011763. COA_CT_C. IPR011762. COA_CT_N. [Graphical view]
Pfam	PF01039. Carboxyl_trans. 1 hit. [Graphical view]
PROSITE	PS50989. COA_CT_CTER. 1 hit. PS50980. COA_CT_NTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MCCB_ARATH

Accession

Primary (citable) accession number: Q9LDD8
Secondary accession number(s): O49501, Q94F35

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 2, 2002
Last sequence update:	October 1, 2000
Last modified:	November 3, 2009
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)