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Protein

Methylcrotonoyl-CoA carboxylase beta chain, mitochondrial

Gene

MCCB

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Carboxyltransferase subunit of the 3-methylcrotonyl-CoA carboxylase, an enzyme that catalyzes the conversion of 3-methylcrotonyl-CoA to 3-methylglutaconyl-CoA, a critical step for leucine and isovaleric acid catabolism.By similarity

Catalytic activityi

ATP + 3-methylcrotonoyl-CoA + HCO3- = ADP + phosphate + 3-methylglutaconyl-CoA.

Pathwayi: L-leucine degradation

This protein is involved in step 2 of the subpathway that synthesizes (S)-3-hydroxy-3-methylglutaryl-CoA from 3-isovaleryl-CoA.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Isovaleryl-CoA dehydrogenase, mitochondrial (IVD)
  2. Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial (MCCA), Methylcrotonoyl-CoA carboxylase beta chain, mitochondrial (MCCB)
  3. no protein annotated in this organism
This subpathway is part of the pathway L-leucine degradation, which is itself part of Amino-acid degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-3-hydroxy-3-methylglutaryl-CoA from 3-isovaleryl-CoA, the pathway L-leucine degradation and in Amino-acid degradation.

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • cobalt ion binding Source: TAIR
  • methylcrotonoyl-CoA carboxylase activity Source: UniProtKB-EC
  • zinc ion binding Source: TAIR

GO - Biological processi

  • leucine catabolic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT4G34030-MONOMER.
BRENDAi6.4.1.4. 399.
ReactomeiR-ATH-196780. Biotin transport and metabolism.
R-ATH-70895. Branched-chain amino acid catabolism.
UniPathwayiUPA00363; UER00861.

Names & Taxonomyi

Protein namesi
Recommended name:
Methylcrotonoyl-CoA carboxylase beta chain, mitochondrial (EC:6.4.1.4)
Short name:
MCCase subunit beta
Alternative name(s):
3-methylcrotonyl-CoA carboxylase 2
3-methylcrotonyl-CoA:carbon dioxide ligase subunit beta
Gene namesi
Name:MCCB
Ordered Locus Names:At4g34030
ORF Names:F17I5.220, F28A23.210
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G34030.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial matrix Source: UniProtKB-SubCell
  • mitochondrion Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2626MitochondrionSequence analysisAdd
BLAST
Chaini27 – 587561Methylcrotonoyl-CoA carboxylase beta chain, mitochondrialPRO_0000000292Add
BLAST

Proteomic databases

PaxDbiQ9LDD8.
PRIDEiQ9LDD8.

Expressioni

Tissue specificityi

In roots, cotyledons, leaves, flowers, ovaries, siliques and embryos.

Gene expression databases

GenevisibleiQ9LDD8. AT.

Interactioni

Subunit structurei

Probably a heterodimer composed of biotin-containing alpha subunits and beta subunits.By similarity

Protein-protein interaction databases

BioGridi14831. 2 interactions.
IntActiQ9LDD8. 1 interaction.
MINTiMINT-8061614.
STRINGi3702.AT4G34030.1.

Structurei

3D structure databases

ProteinModelPortaliQ9LDD8.
SMRiQ9LDD8. Positions 47-587.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini74 – 581508CarboxyltransferaseAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni367 – 39630Acyl-CoA bindingSequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the AccD/PCCB family.Curated
Contains 1 carboxyltransferase domain.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0540. Eukaryota.
COG4799. LUCA.
HOGENOMiHOG000218692.
InParanoidiQ9LDD8.
KOiK01969.
OMAiQCIVVAN.
OrthoDBiEOG093606CI.
PhylomeDBiQ9LDD8.

Family and domain databases

Gene3Di3.90.226.10. 2 hits.
InterProiIPR000022. Carboxyl_trans.
IPR029045. ClpP/crotonase-like_dom.
IPR011763. COA_CT_C.
IPR011762. COA_CT_N.
[Graphical view]
PfamiPF01039. Carboxyl_trans. 1 hit.
[Graphical view]
SUPFAMiSSF52096. SSF52096. 2 hits.
PROSITEiPS50989. COA_CT_CTER. 1 hit.
PS50980. COA_CT_NTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9LDD8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRILGRRVV SASKELTSIQ QWRIRPGTDS RPDPFRTFRG LQKGFCVGIL
60 70 80 90 100
PDGVDRNSEA FSSNSIAMEG ILSELRSHIK KVLAGGGEEA VKRNRSRNKL
110 120 130 140 150
LPRERIDRLL DPGSSFLELS QLAGHELYEE PLPSGGIITG IGPIHGRICM
160 170 180 190 200
FMANDPTVKG GTYYPITIKK HLRAQEIAAR CRLPCIYLVD SGGAYLPKQA
210 220 230 240 250
EVFPDKENFG RVFYNESVMS SDGIPQIAIV LGSCTAGGAY IPAMADESVM
260 270 280 290 300
VKGNGTIFLA GPPLVKAATG EEVSAEDLGG ATVHCTVSGV SDYFAQDELH
310 320 330 340 350
GLAIGRNIVK NLHMAAKQGM EGTFGSKNLV YKEPLYDINE LRSIAPVDHK
360 370 380 390 400
QQFDVRSIIA RIVDGSEFDE FKKQYGTTLV TGFARIYGQT VGIIGNNGIL
410 420 430 440 450
FNESALKGAH FIELCSQRKI PLVFLQNITG FMVGSRAEAN GIAKAGAKMV
460 470 480 490 500
MAVSCAKVPK ITIITGASFG AGNYAMCGRA YSPDFMFIWP NARIGIMGGA
510 520 530 540 550
QAAGVLTQIE RATKKRQGIK WTEEEEEAFK KKTVDAYERE ANPYYSTARL
560 570 580
WDDGVIDPCD TRKVLGLCLS AALNRPLEDT RFGVFRM
Length:587
Mass (Da):64,013
Last modified:October 1, 2000 - v1
Checksum:iBD6E0EEFDC762955
GO

Sequence cautioni

The sequence CAA17569 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence CAA19885 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence CAB80120 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti143 – 1431P → T in AAK62371 (PubMed:14593172).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF059511 Genomic DNA. Translation: AAF35259.1.
AF059510 mRNA. Translation: AAF35258.1.
AL021961 Genomic DNA. Translation: CAA17569.1. Sequence problems.
AL031032 Genomic DNA. Translation: CAA19885.1. Sequence problems.
AL161584 Genomic DNA. Translation: CAB80120.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE86312.1.
AF386926 mRNA. Translation: AAK62371.1.
BT000384 mRNA. Translation: AAN15703.1.
RefSeqiNP_567950.1. NM_119564.4.
UniGeneiAt.19955.

Genome annotation databases

EnsemblPlantsiAT4G34030.1; AT4G34030.1; AT4G34030.
GeneIDi829549.
GrameneiAT4G34030.1; AT4G34030.1; AT4G34030.
KEGGiath:AT4G34030.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF059511 Genomic DNA. Translation: AAF35259.1.
AF059510 mRNA. Translation: AAF35258.1.
AL021961 Genomic DNA. Translation: CAA17569.1. Sequence problems.
AL031032 Genomic DNA. Translation: CAA19885.1. Sequence problems.
AL161584 Genomic DNA. Translation: CAB80120.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE86312.1.
AF386926 mRNA. Translation: AAK62371.1.
BT000384 mRNA. Translation: AAN15703.1.
RefSeqiNP_567950.1. NM_119564.4.
UniGeneiAt.19955.

3D structure databases

ProteinModelPortaliQ9LDD8.
SMRiQ9LDD8. Positions 47-587.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi14831. 2 interactions.
IntActiQ9LDD8. 1 interaction.
MINTiMINT-8061614.
STRINGi3702.AT4G34030.1.

Proteomic databases

PaxDbiQ9LDD8.
PRIDEiQ9LDD8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G34030.1; AT4G34030.1; AT4G34030.
GeneIDi829549.
GrameneiAT4G34030.1; AT4G34030.1; AT4G34030.
KEGGiath:AT4G34030.

Organism-specific databases

TAIRiAT4G34030.

Phylogenomic databases

eggNOGiKOG0540. Eukaryota.
COG4799. LUCA.
HOGENOMiHOG000218692.
InParanoidiQ9LDD8.
KOiK01969.
OMAiQCIVVAN.
OrthoDBiEOG093606CI.
PhylomeDBiQ9LDD8.

Enzyme and pathway databases

UniPathwayiUPA00363; UER00861.
BioCyciARA:AT4G34030-MONOMER.
BRENDAi6.4.1.4. 399.
ReactomeiR-ATH-196780. Biotin transport and metabolism.
R-ATH-70895. Branched-chain amino acid catabolism.

Miscellaneous databases

PROiQ9LDD8.

Gene expression databases

GenevisibleiQ9LDD8. AT.

Family and domain databases

Gene3Di3.90.226.10. 2 hits.
InterProiIPR000022. Carboxyl_trans.
IPR029045. ClpP/crotonase-like_dom.
IPR011763. COA_CT_C.
IPR011762. COA_CT_N.
[Graphical view]
PfamiPF01039. Carboxyl_trans. 1 hit.
[Graphical view]
SUPFAMiSSF52096. SSF52096. 2 hits.
PROSITEiPS50989. COA_CT_CTER. 1 hit.
PS50980. COA_CT_NTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMCCB_ARATH
AccessioniPrimary (citable) accession number: Q9LDD8
Secondary accession number(s): O49501, Q94F35
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 2, 2002
Last sequence update: October 1, 2000
Last modified: September 7, 2016
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Temporal and spatial accumulation of the alpha and beta subunits during development at approximately equal molar ratios.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.