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Q9LDC0

- FKB42_ARATH

UniProt

Q9LDC0 - FKB42_ARATH

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Protein

Peptidyl-prolyl cis-trans isomerase FKBP42

Gene

FKBP42

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides By similarity. Modulates the uptake of MRP substrates into the vacuole; reduces metolachlor-GS (MOC-GS) and enhances 17-beta-estradiol 17-(beta-D-glucuronide) (E217betaG) uptake. Regulates cell elongation and orientation. Functions as a positive regulator of PGP1-mediated auxin transport. Confers drug modulation of PGP1 efflux activity as interaction with NPA or flavonol quercetin prevents its physical and functional interaction with PGP1. Required for the proper localization of auxin-related ABCB transporters. Plays a role in brassinosteroid (BR) signaling pathway.By similarity6 Publications

Catalytic activityi

Peptidylproline (omega=180) = peptidylproline (omega=0).

GO - Molecular functioni

  1. calmodulin binding Source: TAIR
  2. FK506 binding Source: RefGenome
  3. peptidyl-prolyl cis-trans isomerase activity Source: TAIR

GO - Biological processi

  1. auxin-activated signaling pathway Source: UniProtKB-KW
  2. leaf development Source: TAIR
  3. peptidyl-proline modification Source: RefGenome
  4. protein folding Source: UniProtKB-KW
  5. protein peptidyl-prolyl isomerization Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Rotamase

Keywords - Biological processi

Auxin signaling pathway

Keywords - Ligandi

Calmodulin-binding

Enzyme and pathway databases

BioCyciARA:AT3G21640-MONOMER.

Protein family/group databases

TCDBi8.A.11.1.1. the immunophilin-like prolyl:peptidyl isomerase regulator (i-ppi) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Peptidyl-prolyl cis-trans isomerase FKBP42 (EC:5.2.1.8)
Short name:
PPIase FKBP42
Alternative name(s):
42 kDa peptidyl-prolyl isomerase
FK506-binding protein 42
Short name:
AtFKBP42
Immunophilin FKBP42
Protein TWISTED DWARF 1
Protein ULTRACURVATA 2
Rotamase
Gene namesi
Name:FKBP42
Synonyms:TWD1, UCU2
Ordered Locus Names:At3g21640
ORF Names:MIL23.21
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 3

Organism-specific databases

TAIRiAT3G21640.

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB-KW
  2. integral component of membrane Source: UniProtKB-KW
  3. plasma membrane Source: TAIR
  4. vacuole Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endoplasmic reticulum, Membrane, Vacuole

Pathology & Biotechi

Disruption phenotypei

Plants display helical rotation of several organs.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 365365Peptidyl-prolyl cis-trans isomerase FKBP42PRO_0000226087Add
BLAST

Proteomic databases

PaxDbiQ9LDC0.
PRIDEiQ9LDC0.

Expressioni

Gene expression databases

GenevestigatoriQ9LDC0.

Interactioni

Subunit structurei

Interacts with calmodulin (CaM), MRP1, MRP2, MDR1/PGP1, MDR11/PGP19 and SHD/HSP90. Interacts with 1-naphthylphthalamic acid (NPA).4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ABCB1Q9ZR722EBI-360006,EBI-366396
ABCC1Q9C8G94EBI-360006,EBI-637633

Protein-protein interaction databases

BioGridi7050. 5 interactions.
IntActiQ9LDC0. 5 interactions.

Structurei

Secondary structure

1
365
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi45 – 484
Beta strandi51 – 577
Beta strandi69 – 7810
Turni79 – 813
Beta strandi84 – 874
Turni88 – 925
Beta strandi95 – 984
Helixi104 – 1063
Helixi107 – 1137
Beta strandi121 – 1266
Helixi128 – 1303
Turni131 – 1355
Beta strandi137 – 1415
Beta strandi149 – 15911
Turni166 – 1683
Helixi172 – 18817
Beta strandi191 – 1933
Helixi196 – 20813
Helixi211 – 2155
Helixi219 – 22911
Helixi231 – 24111
Turni242 – 2443
Helixi247 – 25913
Helixi264 – 27512
Turni276 – 2783
Helixi280 – 28910

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2F4EX-ray2.32A/B1-180[»]
2IF4X-ray2.85A1-338[»]
ProteinModelPortaliQ9LDC0.
SMRiQ9LDC0. Positions 35-338.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9LDC0.

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei339 – 35719Helical; Anchor for type IV membrane proteinAdd
BLAST

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini67 – 15993PPIase FKBP-typePROSITE-ProRule annotationAdd
BLAST
Repeati179 – 21234TPR 1Add
BLAST
Repeati230 – 26334TPR 2Add
BLAST
Repeati264 – 29734TPR 3Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 163163Interaction with MDR1/PGP1Add
BLAST
Regioni163 – 337175Interaction with MRP1Add
BLAST
Regioni310 – 32617Calmodulin-bindingSequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the FKBP-type PPIase family.Curated
Contains 1 PPIase FKBP-type domain.PROSITE-ProRule annotation
Contains 3 TPR repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, TPR repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0545.
HOGENOMiHOG000006189.
InParanoidiQ9LDC0.
OMAiHSDKEGT.
PhylomeDBiQ9LDC0.

Family and domain databases

Gene3Di1.10.150.160. 1 hit.
1.25.40.10. 1 hit.
InterProiIPR023114. Elongated_TPR_rpt_dom.
IPR023566. PPIase_FKBP.
IPR001179. PPIase_FKBP_dom.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR019734. TPR_repeat.
[Graphical view]
PANTHERiPTHR10516. PTHR10516. 1 hit.
PfamiPF00254. FKBP_C. 1 hit.
PF13181. TPR_8. 1 hit.
[Graphical view]
SMARTiSM00028. TPR. 3 hits.
[Graphical view]
PROSITEiPS50059. FKBP_PPIASE. 1 hit.
PS50005. TPR. 2 hits.
PS50293. TPR_REGION. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9LDC0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDESLEHQTQ THDQESEIVT EGSAVVHSEP SQEGNVPPKV DSEAEVLDEK
60 70 80 90 100
VSKQIIKEGH GSKPSKYSTC FLHYRAWTKN SQHKFEDTWH EQQPIELVLG
110 120 130 140 150
KEKKELAGLA IGVASMKSGE RALVHVGWEL AYGKEGNFSF PNVPPMADLL
160 170 180 190 200
YEVEVIGFDE TKEGKARSDM TVEERIGAAD RRKMDGNSLF KEEKLEEAMQ
210 220 230 240 250
QYEMAIAYMG DDFMFQLYGK YQDMALAVKN PCHLNIAACL IKLKRYDEAI
260 270 280 290 300
GHCNIVLTEE EKNPKALFRR GKAKAELGQM DSARDDFRKA QKYAPDDKAI
310 320 330 340 350
RRELRALAEQ EKALYQKQKE MYKGIFKGKD EGGAKSKSLF WLIVLWQWFV
360
SLFSRIFRRH RVKAD
Length:365
Mass (Da):41,806
Last modified:October 1, 2000 - v1
Checksum:iC939B75EEC79EA87
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti107 – 1071A → P in AAM13008. (PubMed:14593172)Curated
Sequence conflicti107 – 1071A → P in AAN65079. (PubMed:14593172)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ224640 mRNA. Translation: CAC00654.1.
AB019232 Genomic DNA. Translation: BAB02359.1.
CP002686 Genomic DNA. Translation: AEE76533.1.
AY093009 mRNA. Translation: AAM13008.1.
BT001192 mRNA. Translation: AAN65079.1.
RefSeqiNP_188801.2. NM_113059.2.
UniGeneiAt.5664.

Genome annotation databases

EnsemblPlantsiAT3G21640.1; AT3G21640.1; AT3G21640.
GeneIDi821718.
KEGGiath:AT3G21640.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ224640 mRNA. Translation: CAC00654.1 .
AB019232 Genomic DNA. Translation: BAB02359.1 .
CP002686 Genomic DNA. Translation: AEE76533.1 .
AY093009 mRNA. Translation: AAM13008.1 .
BT001192 mRNA. Translation: AAN65079.1 .
RefSeqi NP_188801.2. NM_113059.2.
UniGenei At.5664.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2F4E X-ray 2.32 A/B 1-180 [» ]
2IF4 X-ray 2.85 A 1-338 [» ]
ProteinModelPortali Q9LDC0.
SMRi Q9LDC0. Positions 35-338.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 7050. 5 interactions.
IntActi Q9LDC0. 5 interactions.

Protein family/group databases

TCDBi 8.A.11.1.1. the immunophilin-like prolyl:peptidyl isomerase regulator (i-ppi) family.

Proteomic databases

PaxDbi Q9LDC0.
PRIDEi Q9LDC0.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT3G21640.1 ; AT3G21640.1 ; AT3G21640 .
GeneIDi 821718.
KEGGi ath:AT3G21640.

Organism-specific databases

TAIRi AT3G21640.

Phylogenomic databases

eggNOGi COG0545.
HOGENOMi HOG000006189.
InParanoidi Q9LDC0.
OMAi HSDKEGT.
PhylomeDBi Q9LDC0.

Enzyme and pathway databases

BioCyci ARA:AT3G21640-MONOMER.

Miscellaneous databases

EvolutionaryTracei Q9LDC0.
PROi Q9LDC0.

Gene expression databases

Genevestigatori Q9LDC0.

Family and domain databases

Gene3Di 1.10.150.160. 1 hit.
1.25.40.10. 1 hit.
InterProi IPR023114. Elongated_TPR_rpt_dom.
IPR023566. PPIase_FKBP.
IPR001179. PPIase_FKBP_dom.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR019734. TPR_repeat.
[Graphical view ]
PANTHERi PTHR10516. PTHR10516. 1 hit.
Pfami PF00254. FKBP_C. 1 hit.
PF13181. TPR_8. 1 hit.
[Graphical view ]
SMARTi SM00028. TPR. 3 hits.
[Graphical view ]
PROSITEi PS50059. FKBP_PPIASE. 1 hit.
PS50005. TPR. 2 hits.
PS50293. TPR_REGION. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Structure and evolution of FKBP-like genes in Arabidopsis."
    Kolukisaoglu U., Berger J., Eckhoff A., Moeller A., Saal B., Bellini C., Schulz B.
    Submitted (FEB-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
  2. "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones."
    Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.
    DNA Res. 7:131-135(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Characterization of Arabidopsis thaliana AtFKBP42 that is membrane-bound and interacts with Hsp90."
    Kamphausen T., Fanghaenel J., Neumann D., Schulz B., Rahfeld J.-U.
    Plant J. 32:263-276(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, INTERACTION WITH CALMODULIN AND SHD/HSP90, DISRUPTION PHENOTYPE.
  6. "TWISTED DWARF1, a unique plasma membrane-anchored immunophilin-like protein, interacts with Arabidopsis multidrug resistance-like transporters AtPGP1 and AtPGP19."
    Geisler M., Kolukisaoglu H.U., Bouchard R., Billion K., Berger J., Saal B., Frangne N., Koncz-Kalman Z., Koncz C., Dudler R., Blakeslee J.J., Murphy A.S., Martinoia E., Schulz B.
    Mol. Biol. Cell 14:4238-4249(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH MDR1/PGP1 AND MDR11/PGP19.
  7. "Arabidopsis immunophilin-like TWD1 functionally interacts with vacuolar ABC transporters."
    Geisler M., Girin M., Brandt S., Vincenzetti V., Plaza S., Paris N., Kobae Y., Maeshima M., Billion K., Kolukisaoglu U.H., Schulz B., Martinoia E.
    Mol. Biol. Cell 15:3393-3405(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH MRP1 AND MRP2.
  8. "The ULTRACURVATA2 gene of Arabidopsis encodes an FK506-binding protein involved in auxin and brassinosteroid signaling."
    Perez-Perez J.M., Ponce M.R., Micol J.L.
    Plant Physiol. 134:101-117(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. "Immunophilins and parvulins. Superfamily of peptidyl prolyl isomerases in Arabidopsis."
    He Z., Li L., Luan S.
    Plant Physiol. 134:1248-1267(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  10. "Immunophilin-like TWISTED DWARF1 modulates auxin efflux activities of Arabidopsis P-glycoproteins."
    Bouchard R., Bailly A., Blakeslee J.J., Oehring S.C., Vincenzetti V., Lee O.R., Paponov I., Palme K., Mancuso S., Murphy A.S., Schulz B., Geisler M.
    J. Biol. Chem. 281:30603-30612(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  11. "Modulation of P-glycoproteins by auxin transport inhibitors is mediated by interaction with immunophilins."
    Bailly A., Sovero V., Vincenzetti V., Santelia D., Bartnik D., Koenig B.W., Mancuso S., Martinoia E., Geisler M.
    J. Biol. Chem. 283:21817-21826(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH NPA.
  12. "The ER-localized TWD1 immunophilin is necessary for localization of multidrug resistance-like proteins required for polar auxin transport in Arabidopsis roots."
    Wu G., Otegui M.S., Spalding E.P.
    Plant Cell 22:3295-3304(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  13. "Crystal structure of a plant immunophilin domain involved in regulation of MDR-type ABC transporters."
    Weiergraeber O.H., Eckhoff A., Granzin J.
    FEBS Lett. 580:251-255(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.32 ANGSTROMS) OF 1-180.
  14. "Crystal structure of a multi-domain immunophilin from Arabidopsis thaliana: a paradigm for regulation of plant ABC transporters."
    Granzin J., Eckhoff A., Weiergraber O.H.
    J. Mol. Biol. 364:799-809(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.85 ANGSTROMS) OF 1-338.
  15. "Solid-state NMR characterization of the putative membrane anchor of TWD1 from Arabidopsis thaliana."
    Scheidt H.A., Vogel A., Eckhoff A., Koenig B.W., Huster D.
    Eur. Biophys. J. 36:393-404(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 335-365, MEMBRANE ANCHOR.

Entry informationi

Entry nameiFKB42_ARATH
AccessioniPrimary (citable) accession number: Q9LDC0
Secondary accession number(s): Q8RWM0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: October 1, 2000
Last modified: October 29, 2014
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3