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Protein

Peptidyl-prolyl cis-trans isomerase FKBP42

Gene

FKBP42

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides (By similarity). Modulates the uptake of MRP substrates into the vacuole; reduces metolachlor-GS (MOC-GS) and enhances 17-beta-estradiol 17-(beta-D-glucuronide) (E217betaG) uptake. Regulates cell elongation and orientation. Functions as a positive regulator of PGP1-mediated auxin transport. Confers drug modulation of PGP1 efflux activity as interaction with NPA or flavonol quercetin prevents its physical and functional interaction with PGP1. Required for the proper localization of auxin-related ABCB transporters. Plays a role in brassinosteroid (BR) signaling pathway.By similarity6 Publications

Catalytic activityi

Peptidylproline (omega=180) = peptidylproline (omega=0).

GO - Molecular functioni

  • calmodulin binding Source: TAIR
  • FK506 binding Source: GO_Central
  • peptidyl-prolyl cis-trans isomerase activity Source: TAIR

GO - Biological processi

  • auxin-activated signaling pathway Source: UniProtKB-KW
  • chaperone-mediated protein folding Source: GO_Central
  • leaf development Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Rotamase

Keywords - Biological processi

Auxin signaling pathway

Keywords - Ligandi

Calmodulin-binding

Protein family/group databases

TCDBi8.A.11.1.1. the immunophilin-like prolyl:peptidyl isomerase regulator (i-ppi) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Peptidyl-prolyl cis-trans isomerase FKBP42 (EC:5.2.1.8)
Short name:
PPIase FKBP42
Alternative name(s):
42 kDa peptidyl-prolyl isomerase
FK506-binding protein 42
Short name:
AtFKBP42
Immunophilin FKBP42
Protein TWISTED DWARF 1
Protein ULTRACURVATA 2
Rotamase
Gene namesi
Name:FKBP42
Synonyms:TWD1, UCU2
Ordered Locus Names:At3g21640
ORF Names:MIL23.21
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G21640.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei339 – 357Helical; Anchor for type IV membrane proteinAdd BLAST19

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endoplasmic reticulum, Membrane, Vacuole

Pathology & Biotechi

Disruption phenotypei

Plants display helical rotation of several organs.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002260871 – 365Peptidyl-prolyl cis-trans isomerase FKBP42Add BLAST365

Proteomic databases

PaxDbiQ9LDC0.

Expressioni

Gene expression databases

GenevisibleiQ9LDC0. AT.

Interactioni

Subunit structurei

Interacts with calmodulin (CaM), MRP1, MRP2, MDR1/PGP1, MDR11/PGP19 and SHD/HSP90. Interacts with 1-naphthylphthalamic acid (NPA).4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ABCB1Q9ZR722EBI-360006,EBI-366396
ABCC1Q9C8G94EBI-360006,EBI-637633

GO - Molecular functioni

  • calmodulin binding Source: TAIR

Protein-protein interaction databases

BioGridi7050. 31 interactors.
IntActiQ9LDC0. 5 interactors.
STRINGi3702.AT3G21640.1.

Structurei

Secondary structure

1365
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi45 – 48Combined sources4
Beta strandi51 – 57Combined sources7
Beta strandi69 – 78Combined sources10
Turni79 – 81Combined sources3
Beta strandi84 – 87Combined sources4
Turni88 – 92Combined sources5
Beta strandi95 – 98Combined sources4
Helixi104 – 106Combined sources3
Helixi107 – 113Combined sources7
Beta strandi121 – 126Combined sources6
Helixi128 – 130Combined sources3
Turni131 – 135Combined sources5
Beta strandi137 – 141Combined sources5
Beta strandi149 – 159Combined sources11
Turni166 – 168Combined sources3
Helixi172 – 188Combined sources17
Beta strandi191 – 193Combined sources3
Helixi196 – 208Combined sources13
Helixi211 – 215Combined sources5
Helixi219 – 229Combined sources11
Helixi231 – 241Combined sources11
Turni242 – 244Combined sources3
Helixi247 – 259Combined sources13
Helixi264 – 275Combined sources12
Turni276 – 278Combined sources3
Helixi280 – 289Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2F4EX-ray2.32A/B1-180[»]
2IF4X-ray2.85A1-338[»]
ProteinModelPortaliQ9LDC0.
SMRiQ9LDC0.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9LDC0.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini67 – 159PPIase FKBP-typePROSITE-ProRule annotationAdd BLAST93
Repeati179 – 212TPR 1Add BLAST34
Repeati230 – 263TPR 2Add BLAST34
Repeati264 – 297TPR 3Add BLAST34

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 163Interaction with MDR1/PGP1Add BLAST163
Regioni163 – 337Interaction with MRP11 PublicationAdd BLAST175
Regioni310 – 326Calmodulin-bindingSequence analysisAdd BLAST17

Sequence similaritiesi

Belongs to the FKBP-type PPIase family.Curated
Contains 1 PPIase FKBP-type domain.PROSITE-ProRule annotation
Contains 3 TPR repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, TPR repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0543. Eukaryota.
COG0545. LUCA.
HOGENOMiHOG000006189.
InParanoidiQ9LDC0.
OMAiPCHLNIA.
OrthoDBiEOG09360ESR.
PhylomeDBiQ9LDC0.

Family and domain databases

Gene3Di1.10.150.160. 1 hit.
1.25.40.10. 1 hit.
InterProiIPR023114. Elongated_TPR_rpt_dom.
IPR023566. PPIase_FKBP.
IPR001179. PPIase_FKBP_dom.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR019734. TPR_repeat.
[Graphical view]
PANTHERiPTHR10516. PTHR10516. 1 hit.
PfamiPF00254. FKBP_C. 1 hit.
PF13181. TPR_8. 1 hit.
[Graphical view]
SMARTiSM00028. TPR. 3 hits.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 1 hit.
PROSITEiPS50059. FKBP_PPIASE. 1 hit.
PS50005. TPR. 2 hits.
PS50293. TPR_REGION. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9LDC0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDESLEHQTQ THDQESEIVT EGSAVVHSEP SQEGNVPPKV DSEAEVLDEK
60 70 80 90 100
VSKQIIKEGH GSKPSKYSTC FLHYRAWTKN SQHKFEDTWH EQQPIELVLG
110 120 130 140 150
KEKKELAGLA IGVASMKSGE RALVHVGWEL AYGKEGNFSF PNVPPMADLL
160 170 180 190 200
YEVEVIGFDE TKEGKARSDM TVEERIGAAD RRKMDGNSLF KEEKLEEAMQ
210 220 230 240 250
QYEMAIAYMG DDFMFQLYGK YQDMALAVKN PCHLNIAACL IKLKRYDEAI
260 270 280 290 300
GHCNIVLTEE EKNPKALFRR GKAKAELGQM DSARDDFRKA QKYAPDDKAI
310 320 330 340 350
RRELRALAEQ EKALYQKQKE MYKGIFKGKD EGGAKSKSLF WLIVLWQWFV
360
SLFSRIFRRH RVKAD
Length:365
Mass (Da):41,806
Last modified:October 1, 2000 - v1
Checksum:iC939B75EEC79EA87
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti107A → P in AAM13008 (PubMed:14593172).Curated1
Sequence conflicti107A → P in AAN65079 (PubMed:14593172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ224640 mRNA. Translation: CAC00654.1.
AB019232 Genomic DNA. Translation: BAB02359.1.
CP002686 Genomic DNA. Translation: AEE76533.1.
AY093009 mRNA. Translation: AAM13008.1.
BT001192 mRNA. Translation: AAN65079.1.
RefSeqiNP_188801.2. NM_113059.3.
UniGeneiAt.5664.

Genome annotation databases

EnsemblPlantsiAT3G21640.1; AT3G21640.1; AT3G21640.
GeneIDi821718.
GrameneiAT3G21640.1; AT3G21640.1; AT3G21640.
KEGGiath:AT3G21640.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ224640 mRNA. Translation: CAC00654.1.
AB019232 Genomic DNA. Translation: BAB02359.1.
CP002686 Genomic DNA. Translation: AEE76533.1.
AY093009 mRNA. Translation: AAM13008.1.
BT001192 mRNA. Translation: AAN65079.1.
RefSeqiNP_188801.2. NM_113059.3.
UniGeneiAt.5664.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2F4EX-ray2.32A/B1-180[»]
2IF4X-ray2.85A1-338[»]
ProteinModelPortaliQ9LDC0.
SMRiQ9LDC0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi7050. 31 interactors.
IntActiQ9LDC0. 5 interactors.
STRINGi3702.AT3G21640.1.

Protein family/group databases

TCDBi8.A.11.1.1. the immunophilin-like prolyl:peptidyl isomerase regulator (i-ppi) family.

Proteomic databases

PaxDbiQ9LDC0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G21640.1; AT3G21640.1; AT3G21640.
GeneIDi821718.
GrameneiAT3G21640.1; AT3G21640.1; AT3G21640.
KEGGiath:AT3G21640.

Organism-specific databases

TAIRiAT3G21640.

Phylogenomic databases

eggNOGiKOG0543. Eukaryota.
COG0545. LUCA.
HOGENOMiHOG000006189.
InParanoidiQ9LDC0.
OMAiPCHLNIA.
OrthoDBiEOG09360ESR.
PhylomeDBiQ9LDC0.

Miscellaneous databases

EvolutionaryTraceiQ9LDC0.
PROiQ9LDC0.

Gene expression databases

GenevisibleiQ9LDC0. AT.

Family and domain databases

Gene3Di1.10.150.160. 1 hit.
1.25.40.10. 1 hit.
InterProiIPR023114. Elongated_TPR_rpt_dom.
IPR023566. PPIase_FKBP.
IPR001179. PPIase_FKBP_dom.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR019734. TPR_repeat.
[Graphical view]
PANTHERiPTHR10516. PTHR10516. 1 hit.
PfamiPF00254. FKBP_C. 1 hit.
PF13181. TPR_8. 1 hit.
[Graphical view]
SMARTiSM00028. TPR. 3 hits.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 1 hit.
PROSITEiPS50059. FKBP_PPIASE. 1 hit.
PS50005. TPR. 2 hits.
PS50293. TPR_REGION. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFKB42_ARATH
AccessioniPrimary (citable) accession number: Q9LDC0
Secondary accession number(s): Q8RWM0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: October 1, 2000
Last modified: November 30, 2016
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.