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Protein

Phosphoglycerate kinase 1, chloroplastic

Gene

PGK1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate.

Pathwayi: Calvin cycle

This protein is involved in the pathway Calvin cycle, which is part of Carbohydrate biosynthesis.
View all proteins of this organism that are known to be involved in the pathway Calvin cycle and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei116SubstrateBy similarity1
Binding sitei197SubstrateBy similarity1
Binding sitei230SubstrateBy similarity1
Binding sitei281ATPBy similarity1
Binding sitei372ATP; via carbonyl oxygenBy similarity1
Binding sitei403ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi432 – 435ATPBy similarity4

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • phosphoglycerate kinase activity Source: UniProtKB-EC
  • protein kinase activity Source: TAIR

GO - Biological processi

  • glycolytic process Source: InterPro
  • reductive pentose-phosphate cycle Source: TAIR
  • response to cadmium ion Source: TAIR
  • response to cold Source: TAIR

Keywordsi

Molecular functionKinase, Transferase
Biological processCalvin cycle
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT3G12780-MONOMER
ReactomeiR-ATH-70171 Glycolysis
R-ATH-70263 Gluconeogenesis
UniPathwayiUPA00116

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoglycerate kinase 1, chloroplastic (EC:2.7.2.3)
Gene namesi
Name:PGK1
Ordered Locus Names:At3g12780
ORF Names:MBK21.15
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G12780
TAIRilocus:2087750 AT3G12780

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 75ChloroplastSequence analysisAdd BLAST75
ChainiPRO_000040793076 – 481Phosphoglycerate kinase 1, chloroplasticAdd BLAST406

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei81PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9LD57
PRIDEiQ9LD57

2D gel databases

World-2DPAGE0003:Q9LD57

PTM databases

iPTMnetiQ9LD57

Expressioni

Gene expression databases

ExpressionAtlasiQ9LD57 baseline and differential
GenevisibleiQ9LD57 AT

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

BioGridi5795, 5 interactors
IntActiQ9LD57, 1 interactor
MINTiQ9LD57
STRINGi3702.AT3G12780.1

Structurei

3D structure databases

ProteinModelPortaliQ9LD57
SMRiQ9LD57
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni100 – 102Substrate bindingBy similarity3
Regioni139 – 142Substrate bindingBy similarity4

Sequence similaritiesi

Belongs to the phosphoglycerate kinase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1367 Eukaryota
COG0126 LUCA
HOGENOMiHOG000227107
InParanoidiQ9LD57
KOiK00927
OMAiDMIFDIG
OrthoDBiEOG09360DE5
PhylomeDBiQ9LD57

Family and domain databases

CDDicd00318 Phosphoglycerate_kinase, 1 hit
Gene3Di3.40.50.1260, 3 hits
HAMAPiMF_00145 Phosphoglyc_kinase, 1 hit
InterProiView protein in InterPro
IPR001576 Phosphoglycerate_kinase
IPR015911 Phosphoglycerate_kinase_CS
IPR015824 Phosphoglycerate_kinase_N
IPR036043 Phosphoglycerate_kinase_sf
PANTHERiPTHR11406 PTHR11406, 1 hit
PfamiView protein in Pfam
PF00162 PGK, 1 hit
PIRSFiPIRSF000724 Pgk, 1 hit
PRINTSiPR00477 PHGLYCKINASE
SUPFAMiSSF53748 SSF53748, 1 hit
PROSITEiView protein in PROSITE
PS00111 PGLYCERATE_KINASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9LD57-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASAAASSAF SLLKSTGAVA SSAGTRARAS LLPIPSTSVS ARPLGFSATL
60 70 80 90 100
DSRRFSLHVA SKVESVRGKG SRGVVSMAKK SVGDLTSADL KGKKVFVRAD
110 120 130 140 150
LNVPLDDNQT ITDDTRIRAA IPTIKYLIEN GAKVILSTHL GRPKGVTPKF
160 170 180 190 200
SLAPLVPRLS ELLGIEVTKA DDCIGPEVES LVASLPEGGV LLLENVRFYK
210 220 230 240 250
EEEKNDPEFA KKLASLADLY VNDAFGTAHR AHASTEGVTK FLKPSVAGFL
260 270 280 290 300
LQKELDYLVG AVSNPKRPFA AIVGGSKVSS KIGVIESLLE KCDILLLGGG
310 320 330 340 350
MIFTFYKAQG LSVGSSLVEE DKLELATELL AKAKAKGVSL LLPTDVVVAD
360 370 380 390 400
KFAPDANSKI VPASGIEDGW MGLDIGPDSI KTFNEALDTT QTVIWNGPMG
410 420 430 440 450
VFEMEKFAAG TEAIANKLAE LSEKGVTTII GGGDSVAAVE KVGVAGVMSH
460 470 480
ISTGGGASLE LLEGKVLPGV IALDEAIPVT V
Length:481
Mass (Da):50,112
Last modified:October 1, 2000 - v1
Checksum:iF27EE9BF7F2062EF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF247558 mRNA Translation: AAF70258.1
AB024033 Genomic DNA Translation: BAB02423.1
CP002686 Genomic DNA Translation: AEE75246.1
AF360234 mRNA Translation: AAK25944.1
AF428418 mRNA Translation: AAL16186.1
AY045623 mRNA Translation: AAK73981.1
AY059841 mRNA Translation: AAL24323.1
AY062953 mRNA Translation: AAL33785.1
AY093998 mRNA Translation: AAM16259.1
AY114638 mRNA Translation: AAM47957.1
AY126991 mRNA Translation: AAM83218.1
AK227225 mRNA Translation: BAE99262.1
AK316922 mRNA Translation: BAH19627.1
U37701 mRNA Translation: AAB60303.1
PIRiS71368
RefSeqiNP_187884.1, NM_112114.4
UniGeneiAt.20378
At.53281

Genome annotation databases

EnsemblPlantsiAT3G12780.1; AT3G12780.1; AT3G12780
GeneIDi820461
GrameneiAT3G12780.1; AT3G12780.1; AT3G12780
KEGGiath:AT3G12780

Similar proteinsi

Entry informationi

Entry nameiPGKH1_ARATH
AccessioniPrimary (citable) accession number: Q9LD57
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 3, 2011
Last sequence update: October 1, 2000
Last modified: April 25, 2018
This is version 126 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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