Q9LD57 (PGKH1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoglycerate kinase 1, chloroplastic
EC=2.7.2.3

Gene names

Name:	PGK1
Ordered Locus Names:	At3g12780
ORF Names:	MBK21.15

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	481 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate.
Pathway	Carbohydrate biosynthesis; Calvin cycle.
Subunit structure	Monomer By similarity.
Subcellular location	Plastid › chloroplast Potential.
Sequence similarities	Belongs to the phosphoglycerate kinase family.

Ontologies

Keywords
Biological process	Calvin cycle
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glycolysis Inferred from electronic annotation. Source: InterPro reductive pentose-phosphate cycle Inferred from electronic annotation. Source: UniProtKB-UniPathway response to cadmium ion Inferred from expression pattern PubMed 16502469. Source: TAIR response to cold Inferred from expression pattern PubMed 14617066PubMed 16923014. Source: TAIR
Cellular_component	apoplast Inferred from direct assay PubMed 18538804. Source: TAIR cell wall Inferred from direct assay PubMed 16287169. Source: TAIR chloroplast envelope Inferred from direct assay PubMed 12766230. Source: TAIR chloroplast stroma Inferred from direct assay PubMed 16207701PubMed 18633119PubMed 20061580. Source: TAIR cytosol Inferred from direct assay PubMed 21166475. Source: TAIR membrane Inferred from direct assay PubMed 17432890. Source: TAIR mitochondrion Inferred from direct assay PubMed 14671022. Source: TAIR nucleus Inferred from direct assay PubMed 14617066. Source: TAIR stromule Inferred from direct assay PubMed 16923014. Source: TAIR thylakoid Inferred from direct assay PubMed 16648217. Source: TAIR
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoglycerate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 75	75	Chloroplast Potential
	Chain	76 – 481	406	Phosphoglycerate kinase 1, chloroplastic		PRO_0000407930
Regions
	Nucleotide binding	432 – 435	4	ATP By similarity
	Region	100 – 102	3	Substrate binding By similarity
	Region	139 – 142	4	Substrate binding By similarity
Sites
	Binding site	116	1	Substrate By similarity
	Binding site	197	1	Substrate By similarity
	Binding site	230	1	Substrate By similarity
	Binding site	281	1	ATP By similarity
	Binding site	372	1	ATP; via carbonyl oxygen By similarity
	Binding site	403	1	ATP By similarity
Amino acid modifications
	Modified residue	81	1	Phosphoserine Ref.8

Sequences

	Sequence		Length	Mass (Da)
	Q9LD57 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: F27EE9BF7F2062EF	FASTA	481	50,112
	10 20 30 40 50 60 MASAAASSAF SLLKSTGAVA SSAGTRARAS LLPIPSTSVS ARPLGFSATL DSRRFSLHVA 70 80 90 100 110 120 SKVESVRGKG SRGVVSMAKK SVGDLTSADL KGKKVFVRAD LNVPLDDNQT ITDDTRIRAA 130 140 150 160 170 180 IPTIKYLIEN GAKVILSTHL GRPKGVTPKF SLAPLVPRLS ELLGIEVTKA DDCIGPEVES 190 200 210 220 230 240 LVASLPEGGV LLLENVRFYK EEEKNDPEFA KKLASLADLY VNDAFGTAHR AHASTEGVTK 250 260 270 280 290 300 FLKPSVAGFL LQKELDYLVG AVSNPKRPFA AIVGGSKVSS KIGVIESLLE KCDILLLGGG 310 320 330 340 350 360 MIFTFYKAQG LSVGSSLVEE DKLELATELL AKAKAKGVSL LLPTDVVVAD KFAPDANSKI 370 380 390 400 410 420 VPASGIEDGW MGLDIGPDSI KTFNEALDTT QTVIWNGPMG VFEMEKFAAG TEAIANKLAE 430 440 450 460 470 480 LSEKGVTTII GGGDSVAAVE KVGVAGVMSH ISTGGGASLE LLEGKVLPGV IALDEAIPVT V

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure and regulation of nuclear genes encoding chloroplast and cytosolic phosphoglycerate kinase in Arabidopsis thaliana." Shih M.-C. Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones." Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S. DNA Res. 7:131-135(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[6]	"Analysis of multiple occurrences of alternative splicing events in Arabidopsis thaliana using novel sequenced full-length cDNAs." Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M., Shinozaki K. DNA Res. 16:155-164(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia. Tissue: Rosette leaf.
[7]	"Two phosphoglycerate kinase cDNAs from Arabidopsis thaliana." Loebler M. DNA Seq. 8:247-252(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 83-481. Strain: cv. Columbia. Tissue: Leaf.
[8]	"Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks." Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A., Grossmann J., Gruissem W., Baginsky S. Plant Physiol. 150:889-903(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-81, MASS SPECTROMETRY. Strain: cv. Columbia. Tissue: Seedling.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF247558 mRNA. Translation: AAF70258.1. AB024033 Genomic DNA. Translation: BAB02423.1. CP002686 Genomic DNA. Translation: AEE75246.1. AF360234 mRNA. Translation: AAK25944.1. AF428418 mRNA. Translation: AAL16186.1. AY045623 mRNA. Translation: AAK73981.1. AY059841 mRNA. Translation: AAL24323.1. AY062953 mRNA. Translation: AAL33785.1. AY093998 mRNA. Translation: AAM16259.1. AY114638 mRNA. Translation: AAM47957.1. AY126991 mRNA. Translation: AAM83218.1. AK227225 mRNA. Translation: BAE99262.1. AK316922 mRNA. Translation: BAH19627.1. U37701 mRNA. Translation: AAB60303.1.
IPI	IPI00535490.
PIR	S71368.
RefSeq	NP_187884.1. NM_112114.3.
UniGene	At.20378. At.53281.
3D structure databases
ProteinModelPortal	Q9LD57.
SMR	Q9LD57. Positions 77-475.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT3G12780.1-P.
2D gel databases
World-2DPAGE	0003:Q9LD57.
Proteomic databases
PaxDb	Q9LD57.
PRIDE	Q9LD57.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT3G12780.1; AT3G12780.1; AT3G12780.
GeneID	820461.
KEGG	ath:AT3G12780.
Organism-specific databases
TAIR	At3g12780.
Phylogenomic databases
eggNOG	COG0126.
HOGENOM	HOG000227107.
InParanoid	Q9LD57.
KO	K00927.
OMA	KFGLADK.
PhylomeDB	Q9LD57.
ProtClustDB	PLN03034.
Enzyme and pathway databases
UniPathway	UPA00116.
Gene expression databases
Genevestigator	Q9LD57.
Family and domain databases
Gene3D	3.40.50.1260. 1 hit. 3.40.50.1270. 1 hit.
InterPro	IPR001576. Phosphoglycerate_kinase. IPR015901. Phosphoglycerate_kinase_C. IPR015911. Phosphoglycerate_kinase_CS. IPR015824. Phosphoglycerate_kinase_N. [Graphical view]
PANTHER	PTHR11406. PTHR11406. 1 hit.
Pfam	PF00162. PGK. 1 hit. [Graphical view]
PIRSF	PIRSF000724. Pgk. 1 hit.
PRINTS	PR00477. PHGLYCKINASE.
SUPFAM	SSF53748. PGK. 1 hit.
PROSITE	PS00111. PGLYCERATE_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PGKH1_ARATH

Accession

Primary (citable) accession number: Q9LD57

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 3, 2011
Last sequence update:	October 1, 2000
Last modified:	May 29, 2013
This is version 93 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program