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Q9LD57 (PGKH1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoglycerate kinase 1, chloroplastic

EC=2.7.2.3
Gene names
Name:PGK1
Ordered Locus Names:At3g12780
ORF Names:MBK21.15
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length481 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate. HAMAP-Rule MF_00145

Pathway

Carbohydrate biosynthesis; Calvin cycle. HAMAP-Rule MF_00145

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00145

Subcellular location

Plastidchloroplast Potential HAMAP-Rule MF_00145.

Sequence similarities

Belongs to the phosphoglycerate kinase family.

Ontologies

Keywords
   Biological processCalvin cycle
   Cellular componentChloroplast
Plastid
   DomainTransit peptide
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processglycolysis

Inferred from electronic annotation. Source: InterPro

reductive pentose-phosphate cycle

Inferred from electronic annotation. Source: UniProtKB-UniPathway

response to cadmium ion

Inferred from expression pattern PubMed 16502469. Source: TAIR

response to cold

Inferred from expression pattern PubMed 14617066PubMed 16923014. Source: TAIR

   Cellular_componentapoplast

Inferred from direct assay PubMed 18538804. Source: TAIR

cell wall

Inferred from direct assay PubMed 16287169. Source: TAIR

chloroplast

Inferred from direct assay PubMed 15028209PubMed 18431481. Source: TAIR

chloroplast envelope

Inferred from direct assay PubMed 12766230. Source: TAIR

chloroplast stroma

Inferred from direct assay PubMed 16207701PubMed 18633119PubMed 20061580. Source: TAIR

cytoplasm

Inferred from direct assay PubMed 15610358. Source: TAIR

cytosol

Inferred from direct assay PubMed 21166475. Source: TAIR

membrane

Inferred from direct assay PubMed 17432890. Source: TAIR

mitochondrion

Inferred from direct assay PubMed 14671022. Source: TAIR

nucleus

Inferred from direct assay PubMed 14617066. Source: TAIR

stromule

Inferred from direct assay PubMed 16923014. Source: TAIR

thylakoid

Inferred from direct assay PubMed 16648217. Source: TAIR

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

phosphoglycerate kinase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 7575Chloroplast Potential
Chain76 – 481406Phosphoglycerate kinase 1, chloroplastic HAMAP-Rule MF_00145
PRO_0000407930

Regions

Nucleotide binding432 – 4354ATP By similarity
Region100 – 1023Substrate binding By similarity
Region139 – 1424Substrate binding By similarity

Sites

Binding site1161Substrate By similarity
Binding site1971Substrate By similarity
Binding site2301Substrate By similarity
Binding site2811ATP By similarity
Binding site3721ATP; via carbonyl oxygen By similarity
Binding site4031ATP By similarity

Amino acid modifications

Modified residue811Phosphoserine Ref.8

Sequences

Sequence LengthMass (Da)Tools
Q9LD57 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: F27EE9BF7F2062EF

FASTA48150,112
        10         20         30         40         50         60 
MASAAASSAF SLLKSTGAVA SSAGTRARAS LLPIPSTSVS ARPLGFSATL DSRRFSLHVA 

        70         80         90        100        110        120 
SKVESVRGKG SRGVVSMAKK SVGDLTSADL KGKKVFVRAD LNVPLDDNQT ITDDTRIRAA 

       130        140        150        160        170        180 
IPTIKYLIEN GAKVILSTHL GRPKGVTPKF SLAPLVPRLS ELLGIEVTKA DDCIGPEVES 

       190        200        210        220        230        240 
LVASLPEGGV LLLENVRFYK EEEKNDPEFA KKLASLADLY VNDAFGTAHR AHASTEGVTK 

       250        260        270        280        290        300 
FLKPSVAGFL LQKELDYLVG AVSNPKRPFA AIVGGSKVSS KIGVIESLLE KCDILLLGGG 

       310        320        330        340        350        360 
MIFTFYKAQG LSVGSSLVEE DKLELATELL AKAKAKGVSL LLPTDVVVAD KFAPDANSKI 

       370        380        390        400        410        420 
VPASGIEDGW MGLDIGPDSI KTFNEALDTT QTVIWNGPMG VFEMEKFAAG TEAIANKLAE 

       430        440        450        460        470        480 
LSEKGVTTII GGGDSVAAVE KVGVAGVMSH ISTGGGASLE LLEGKVLPGV IALDEAIPVT 


V 

« Hide

References

« Hide 'large scale' references
[1]"Structure and regulation of nuclear genes encoding chloroplast and cytosolic phosphoglycerate kinase in Arabidopsis thaliana."
Shih M.-C.
Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones."
Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.
DNA Res. 7:131-135(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. expand/collapse author list , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[6]"Analysis of multiple occurrences of alternative splicing events in Arabidopsis thaliana using novel sequenced full-length cDNAs."
Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M., Shinozaki K.
DNA Res. 16:155-164(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
Tissue: Rosette leaf.
[7]"Two phosphoglycerate kinase cDNAs from Arabidopsis thaliana."
Loebler M.
DNA Seq. 8:247-252(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 83-481.
Strain: cv. Columbia.
Tissue: Leaf.
[8]"Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks."
Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A., Grossmann J., Gruissem W., Baginsky S.
Plant Physiol. 150:889-903(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-81, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF247558 mRNA. Translation: AAF70258.1.
AB024033 Genomic DNA. Translation: BAB02423.1.
CP002686 Genomic DNA. Translation: AEE75246.1.
AF360234 mRNA. Translation: AAK25944.1.
AF428418 mRNA. Translation: AAL16186.1.
AY045623 mRNA. Translation: AAK73981.1.
AY059841 mRNA. Translation: AAL24323.1.
AY062953 mRNA. Translation: AAL33785.1.
AY093998 mRNA. Translation: AAM16259.1.
AY114638 mRNA. Translation: AAM47957.1.
AY126991 mRNA. Translation: AAM83218.1.
AK227225 mRNA. Translation: BAE99262.1.
AK316922 mRNA. Translation: BAH19627.1.
U37701 mRNA. Translation: AAB60303.1.
PIRS71368.
RefSeqNP_187884.1. NM_112114.3.
UniGeneAt.20378.
At.53281.

3D structure databases

ProteinModelPortalQ9LD57.
SMRQ9LD57. Positions 77-475.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid5795. 5 interactions.
STRING3702.AT3G12780.1-P.

2D gel databases

World-2DPAGE0003:Q9LD57.

Proteomic databases

PaxDbQ9LD57.
PRIDEQ9LD57.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT3G12780.1; AT3G12780.1; AT3G12780.
GeneID820461.
KEGGath:AT3G12780.

Organism-specific databases

TAIRAT3G12780.

Phylogenomic databases

eggNOGCOG0126.
HOGENOMHOG000227107.
InParanoidQ9LD57.
KOK00927.
OMAFGLADKM.
PhylomeDBQ9LD57.
ProtClustDBPLN03034.

Enzyme and pathway databases

BioCycARA:AT3G12780-MONOMER.
UniPathwayUPA00116.

Gene expression databases

GenevestigatorQ9LD57.

Family and domain databases

Gene3D3.40.50.1260. 1 hit.
3.40.50.1270. 1 hit.
HAMAPMF_00145. Phosphoglyc_kinase.
InterProIPR001576. Phosphoglycerate_kinase.
IPR015901. Phosphoglycerate_kinase_C.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
[Graphical view]
PANTHERPTHR11406. PTHR11406. 1 hit.
PfamPF00162. PGK. 1 hit.
[Graphical view]
PIRSFPIRSF000724. Pgk. 1 hit.
PRINTSPR00477. PHGLYCKINASE.
SUPFAMSSF53748. SSF53748. 1 hit.
PROSITEPS00111. PGLYCERATE_KINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePGKH1_ARATH
AccessionPrimary (citable) accession number: Q9LD57
Entry history
Integrated into UniProtKB/Swiss-Prot: May 3, 2011
Last sequence update: October 1, 2000
Last modified: April 16, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names