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Protein

Phosphoglycerate kinase 1, chloroplastic

Gene

PGK1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate.

Pathwayi: Calvin cycle

This protein is involved in the pathway Calvin cycle, which is part of Carbohydrate biosynthesis.
View all proteins of this organism that are known to be involved in the pathway Calvin cycle and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei116SubstrateBy similarity1
Binding sitei197SubstrateBy similarity1
Binding sitei230SubstrateBy similarity1
Binding sitei281ATPBy similarity1
Binding sitei372ATP; via carbonyl oxygenBy similarity1
Binding sitei403ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi432 – 435ATPBy similarity4

GO - Molecular functioni

GO - Biological processi

  • glycolytic process Source: InterPro
  • reductive pentose-phosphate cycle Source: UniProtKB-UniPathway
  • response to cadmium ion Source: TAIR
  • response to cold Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Calvin cycle

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT3G12780-MONOMER.
ReactomeiR-ATH-70171. Glycolysis.
R-ATH-70263. Gluconeogenesis.
UniPathwayiUPA00116.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoglycerate kinase 1, chloroplastic (EC:2.7.2.3)
Gene namesi
Name:PGK1
Ordered Locus Names:At3g12780
ORF Names:MBK21.15
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G12780.

Subcellular locationi

GO - Cellular componenti

  • apoplast Source: TAIR
  • cell wall Source: TAIR
  • chloroplast Source: TAIR
  • chloroplast envelope Source: TAIR
  • chloroplast stroma Source: TAIR
  • cytoplasm Source: TAIR
  • cytosol Source: TAIR
  • membrane Source: TAIR
  • mitochondrion Source: TAIR
  • nucleus Source: TAIR
  • stromule Source: TAIR
  • thylakoid Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 75ChloroplastSequence analysisAdd BLAST75
ChainiPRO_000040793076 – 481Phosphoglycerate kinase 1, chloroplasticAdd BLAST406

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei81PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9LD57.
PRIDEiQ9LD57.

2D gel databases

World-2DPAGE0003:Q9LD57.

PTM databases

iPTMnetiQ9LD57.

Expressioni

Gene expression databases

GenevisibleiQ9LD57. AT.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

BioGridi5795. 5 interactors.
IntActiQ9LD57. 1 interactor.
STRINGi3702.AT3G12780.1.

Structurei

3D structure databases

ProteinModelPortaliQ9LD57.
SMRiQ9LD57.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni100 – 102Substrate bindingBy similarity3
Regioni139 – 142Substrate bindingBy similarity4

Sequence similaritiesi

Belongs to the phosphoglycerate kinase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1367. Eukaryota.
COG0126. LUCA.
HOGENOMiHOG000227107.
InParanoidiQ9LD57.
KOiK00927.
OMAiSITYLSG.
OrthoDBiEOG09360DE5.
PhylomeDBiQ9LD57.

Family and domain databases

CDDicd00318. Phosphoglycerate_kinase. 1 hit.
Gene3Di3.40.50.1260. 1 hit.
3.40.50.1270. 1 hit.
HAMAPiMF_00145. Phosphoglyc_kinase. 1 hit.
InterProiIPR001576. Phosphoglycerate_kinase.
IPR015901. Phosphoglycerate_kinase_C.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
[Graphical view]
PANTHERiPTHR11406. PTHR11406. 1 hit.
PfamiPF00162. PGK. 1 hit.
[Graphical view]
PIRSFiPIRSF000724. Pgk. 1 hit.
PRINTSiPR00477. PHGLYCKINASE.
SUPFAMiSSF53748. SSF53748. 1 hit.
PROSITEiPS00111. PGLYCERATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9LD57-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASAAASSAF SLLKSTGAVA SSAGTRARAS LLPIPSTSVS ARPLGFSATL
60 70 80 90 100
DSRRFSLHVA SKVESVRGKG SRGVVSMAKK SVGDLTSADL KGKKVFVRAD
110 120 130 140 150
LNVPLDDNQT ITDDTRIRAA IPTIKYLIEN GAKVILSTHL GRPKGVTPKF
160 170 180 190 200
SLAPLVPRLS ELLGIEVTKA DDCIGPEVES LVASLPEGGV LLLENVRFYK
210 220 230 240 250
EEEKNDPEFA KKLASLADLY VNDAFGTAHR AHASTEGVTK FLKPSVAGFL
260 270 280 290 300
LQKELDYLVG AVSNPKRPFA AIVGGSKVSS KIGVIESLLE KCDILLLGGG
310 320 330 340 350
MIFTFYKAQG LSVGSSLVEE DKLELATELL AKAKAKGVSL LLPTDVVVAD
360 370 380 390 400
KFAPDANSKI VPASGIEDGW MGLDIGPDSI KTFNEALDTT QTVIWNGPMG
410 420 430 440 450
VFEMEKFAAG TEAIANKLAE LSEKGVTTII GGGDSVAAVE KVGVAGVMSH
460 470 480
ISTGGGASLE LLEGKVLPGV IALDEAIPVT V
Length:481
Mass (Da):50,112
Last modified:October 1, 2000 - v1
Checksum:iF27EE9BF7F2062EF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF247558 mRNA. Translation: AAF70258.1.
AB024033 Genomic DNA. Translation: BAB02423.1.
CP002686 Genomic DNA. Translation: AEE75246.1.
AF360234 mRNA. Translation: AAK25944.1.
AF428418 mRNA. Translation: AAL16186.1.
AY045623 mRNA. Translation: AAK73981.1.
AY059841 mRNA. Translation: AAL24323.1.
AY062953 mRNA. Translation: AAL33785.1.
AY093998 mRNA. Translation: AAM16259.1.
AY114638 mRNA. Translation: AAM47957.1.
AY126991 mRNA. Translation: AAM83218.1.
AK227225 mRNA. Translation: BAE99262.1.
AK316922 mRNA. Translation: BAH19627.1.
U37701 mRNA. Translation: AAB60303.1.
PIRiS71368.
RefSeqiNP_187884.1. NM_112114.4.
UniGeneiAt.20378.
At.53281.

Genome annotation databases

EnsemblPlantsiAT3G12780.1; AT3G12780.1; AT3G12780.
GeneIDi820461.
GrameneiAT3G12780.1; AT3G12780.1; AT3G12780.
KEGGiath:AT3G12780.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF247558 mRNA. Translation: AAF70258.1.
AB024033 Genomic DNA. Translation: BAB02423.1.
CP002686 Genomic DNA. Translation: AEE75246.1.
AF360234 mRNA. Translation: AAK25944.1.
AF428418 mRNA. Translation: AAL16186.1.
AY045623 mRNA. Translation: AAK73981.1.
AY059841 mRNA. Translation: AAL24323.1.
AY062953 mRNA. Translation: AAL33785.1.
AY093998 mRNA. Translation: AAM16259.1.
AY114638 mRNA. Translation: AAM47957.1.
AY126991 mRNA. Translation: AAM83218.1.
AK227225 mRNA. Translation: BAE99262.1.
AK316922 mRNA. Translation: BAH19627.1.
U37701 mRNA. Translation: AAB60303.1.
PIRiS71368.
RefSeqiNP_187884.1. NM_112114.4.
UniGeneiAt.20378.
At.53281.

3D structure databases

ProteinModelPortaliQ9LD57.
SMRiQ9LD57.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi5795. 5 interactors.
IntActiQ9LD57. 1 interactor.
STRINGi3702.AT3G12780.1.

PTM databases

iPTMnetiQ9LD57.

2D gel databases

World-2DPAGE0003:Q9LD57.

Proteomic databases

PaxDbiQ9LD57.
PRIDEiQ9LD57.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G12780.1; AT3G12780.1; AT3G12780.
GeneIDi820461.
GrameneiAT3G12780.1; AT3G12780.1; AT3G12780.
KEGGiath:AT3G12780.

Organism-specific databases

TAIRiAT3G12780.

Phylogenomic databases

eggNOGiKOG1367. Eukaryota.
COG0126. LUCA.
HOGENOMiHOG000227107.
InParanoidiQ9LD57.
KOiK00927.
OMAiSITYLSG.
OrthoDBiEOG09360DE5.
PhylomeDBiQ9LD57.

Enzyme and pathway databases

UniPathwayiUPA00116.
BioCyciARA:AT3G12780-MONOMER.
ReactomeiR-ATH-70171. Glycolysis.
R-ATH-70263. Gluconeogenesis.

Miscellaneous databases

PROiQ9LD57.

Gene expression databases

GenevisibleiQ9LD57. AT.

Family and domain databases

CDDicd00318. Phosphoglycerate_kinase. 1 hit.
Gene3Di3.40.50.1260. 1 hit.
3.40.50.1270. 1 hit.
HAMAPiMF_00145. Phosphoglyc_kinase. 1 hit.
InterProiIPR001576. Phosphoglycerate_kinase.
IPR015901. Phosphoglycerate_kinase_C.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
[Graphical view]
PANTHERiPTHR11406. PTHR11406. 1 hit.
PfamiPF00162. PGK. 1 hit.
[Graphical view]
PIRSFiPIRSF000724. Pgk. 1 hit.
PRINTSiPR00477. PHGLYCKINASE.
SUPFAMiSSF53748. SSF53748. 1 hit.
PROSITEiPS00111. PGLYCERATE_KINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPGKH1_ARATH
AccessioniPrimary (citable) accession number: Q9LD57
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 3, 2011
Last sequence update: October 1, 2000
Last modified: November 30, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.