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Protein

Eukaryotic translation initiation factor 3 subunit A

Gene

TIF3A1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome.UniRule annotation

GO - Molecular functioni

  • translation initiation factor activity Source: TAIR

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Initiation factor

Keywords - Biological processi

Protein biosynthesis

Enzyme and pathway databases

ReactomeiR-ATH-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-ATH-72689. Formation of a pool of free 40S subunits.
R-ATH-72695. Formation of the ternary complex, and subsequently, the 43S complex.
R-ATH-72702. Ribosomal scanning and start codon recognition.

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 3 subunit AUniRule annotation
Short name:
eIF3aUniRule annotation
Alternative name(s):
Eukaryotic translation initiation factor 3 large subunit
Eukaryotic translation initiation factor 3 subunit 10UniRule annotation
eIF-3-thetaUniRule annotation
p114
Gene namesi
Name:TIF3A1
Ordered Locus Names:At4g11420
ORF Names:F25E4.40
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G11420.

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

  • cytosol Source: TAIR
  • eukaryotic 43S preinitiation complex Source: UniProtKB-HAMAP
  • eukaryotic 48S preinitiation complex Source: UniProtKB-HAMAP
  • eukaryotic translation initiation factor 3 complex Source: TAIR
  • plasma membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 987987Eukaryotic translation initiation factor 3 subunit APRO_0000123540Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki61 – 61Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiQ9LD55.
PRIDEiQ9LD55.

PTM databases

iPTMnetiQ9LD55.
SwissPalmiQ9LD55.

Expressioni

Gene expression databases

ExpressionAtlasiQ9LD55. baseline and differential.
GenevisibleiQ9LD55. AT.

Interactioni

Subunit structurei

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex. Binds to the translation initiation factor TIF3H1 (PubMed:15548739).UniRule annotation1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
At5g61200F4K2102EBI-7217371,EBI-7216904

Protein-protein interaction databases

BioGridi12045. 5 interactions.
IntActiQ9LD55. 1 interaction.
MINTiMINT-7980004.
STRINGi3702.AT4G11420.1.

Structurei

3D structure databases

ProteinModelPortaliQ9LD55.
SMRiQ9LD55. Positions 6-599.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini373 – 510138PCIUniRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili93 – 12230UniRule annotationAdd
BLAST
Coiled coili556 – 742187UniRule annotationAdd
BLAST
Coiled coili797 – 85862UniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the eIF-3 subunit A family.UniRule annotation
Contains 1 PCI domain.UniRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG2072. Eukaryota.
ENOG410XQ37. LUCA.
HOGENOMiHOG000246822.
InParanoidiQ9LD55.
KOiK03254.
OMAiAREDSWG.
PhylomeDBiQ9LD55.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
HAMAPiMF_03000. eIF3a.
InterProiIPR027512. EIF3A.
IPR000717. PCI_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF01399. PCI. 1 hit.
[Graphical view]
SMARTiSM00088. PINT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9LD55-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANFAKPENA LKRADELINV GQKQDALQAL HDLITSKRYR AWQKPLEKIM
60 70 80 90 100
FKYLDLCVDL KRGRFAKDGL IQYRIVCQQV NVSSLEEVIK HFLHLATDKA
110 120 130 140 150
EQARSQADAL EEALDVDDLE ADRKPEDLQL SIVSGEKGKD RSDRELVTPW
160 170 180 190 200
FKFLWETYRT VLEILRNNSK LEALYAMTAH KAFQFCKQYK RTTEFRRLCE
210 220 230 240 250
IIRNHLANLN KYRDQRDRPD LSAPESLQLY LDTRFDQLKV ATELGLWQEA
260 270 280 290 300
FRSVEDIYGL MCMVKKTPKS SLLMVYYSKL TEIFWISSSH LYHAYAWFKL
310 320 330 340 350
FSLQKNFNKN LSQKDLQLIA SSVVLAALSI PPFDRAQSAS HMELENEKER
360 370 380 390 400
NLRMANLIGF NLEPKFEGKD MLSRSALLSE LVSKGVLSCA SQEVKDLFHV
410 420 430 440 450
LEHEFHPLDL GSKIQPLLEK ISKSGGKLSS APSLPEVQLS QYVPSLEKLA
460 470 480 490 500
TLRLLQQVSK IYQTIRIESL SQLVPFFQFS EVEKISVDAV KNNFVAMKVD
510 520 530 540 550
HMKGVVIFGN LGIESDGLRD HLAVFAESLS KVRAMLYPVP SKASKLAGVI
560 570 580 590 600
PNLADTVEKE HKRLLARKSI IEKRKEDQER QQLEMEREEE QKRLKLQKLT
610 620 630 640 650
EEAEQKRLAA ELAERRKQRI LREIEEKELE EAQALLEETE KRMKKGKKKP
660 670 680 690 700
LLDGEKVTKQ SVKERALTEQ LKERQEMEKK LQKLAKTMDY LERAKREEAA
710 720 730 740 750
PLIEAAYQRR LVEEREFYER EQQREVELSK ERHESDLKEK NRLSRMLGNK
760 770 780 790 800
EIFQAQVISR RQAEFDRIRT EREERISKII REKKQERDIK RKQIYYLKIE
810 820 830 840 850
EERIRKLQEE EEARKQEEAE RLKKVEAERK ANLDKAFEKQ RQREIELEEK
860 870 880 890 900
SRREREELLR GTNAPPARLA EPTVTPVGTT APAAAAAAAG APAAPYVPKW
910 920 930 940 950
KRQTTEVSGP SAPTSSETDR RSNRGPPPGD DHWGSNRGAA QNTDRWTSNR
960 970 980
ERSGPPAEGG DRWGSGPRGS DDRRSTFGSS RPRPTQR
Length:987
Mass (Da):114,300
Last modified:October 1, 2000 - v1
Checksum:iF38BA715209D55FB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF291711 mRNA. Translation: AAG53635.1.
AL050399 Genomic DNA. Translation: CAB82147.1.
AL161531 Genomic DNA. Translation: CAB81243.1.
CP002687 Genomic DNA. Translation: AEE83010.1.
PIRiT10562.
RefSeqiNP_192881.1. NM_117213.3.
UniGeneiAt.24381.

Genome annotation databases

EnsemblPlantsiAT4G11420.1; AT4G11420.1; AT4G11420.
GeneIDi826746.
GrameneiAT4G11420.1; AT4G11420.1; AT4G11420.
KEGGiath:AT4G11420.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF291711 mRNA. Translation: AAG53635.1.
AL050399 Genomic DNA. Translation: CAB82147.1.
AL161531 Genomic DNA. Translation: CAB81243.1.
CP002687 Genomic DNA. Translation: AEE83010.1.
PIRiT10562.
RefSeqiNP_192881.1. NM_117213.3.
UniGeneiAt.24381.

3D structure databases

ProteinModelPortaliQ9LD55.
SMRiQ9LD55. Positions 6-599.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi12045. 5 interactions.
IntActiQ9LD55. 1 interaction.
MINTiMINT-7980004.
STRINGi3702.AT4G11420.1.

PTM databases

iPTMnetiQ9LD55.
SwissPalmiQ9LD55.

Proteomic databases

PaxDbiQ9LD55.
PRIDEiQ9LD55.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G11420.1; AT4G11420.1; AT4G11420.
GeneIDi826746.
GrameneiAT4G11420.1; AT4G11420.1; AT4G11420.
KEGGiath:AT4G11420.

Organism-specific databases

TAIRiAT4G11420.

Phylogenomic databases

eggNOGiKOG2072. Eukaryota.
ENOG410XQ37. LUCA.
HOGENOMiHOG000246822.
InParanoidiQ9LD55.
KOiK03254.
OMAiAREDSWG.
PhylomeDBiQ9LD55.

Enzyme and pathway databases

ReactomeiR-ATH-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-ATH-72689. Formation of a pool of free 40S subunits.
R-ATH-72695. Formation of the ternary complex, and subsequently, the 43S complex.
R-ATH-72702. Ribosomal scanning and start codon recognition.

Miscellaneous databases

PROiQ9LD55.

Gene expression databases

ExpressionAtlasiQ9LD55. baseline and differential.
GenevisibleiQ9LD55. AT.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
HAMAPiMF_03000. eIF3a.
InterProiIPR027512. EIF3A.
IPR000717. PCI_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF01399. PCI. 1 hit.
[Graphical view]
SMARTiSM00088. PINT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Plant initiation factor 3 subunit composition resembles mammalian initiation factor 3 and has a novel subunit."
    Burks E.A., Bezerra P.P., Le H., Gallie D.R., Browning K.S.
    J. Biol. Chem. 276:2122-2131(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
  2. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
    Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
    , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
    Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Translational regulation via 5' mRNA leader sequences revealed by mutational analysis of the Arabidopsis translation initiation factor subunit eIF3h."
    Kim T.-H., Kim B.-H., Yahalom A., Chamovitz D.A., von Arnim A.G.
    Plant Cell 16:3341-3356(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH TIF3H1.
  5. "Multidimensional protein identification technology (MudPIT) analysis of ubiquitinated proteins in plants."
    Maor R., Jones A., Nuehse T.S., Studholme D.J., Peck S.C., Shirasu K.
    Mol. Cell. Proteomics 6:601-610(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-61.
    Strain: cv. Landsberg erecta.

Entry informationi

Entry nameiEIF3A_ARATH
AccessioniPrimary (citable) accession number: Q9LD55
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2002
Last sequence update: October 1, 2000
Last modified: February 17, 2016
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Translation initiation factors
    List of translation initiation factor entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.