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Protein

Copper chaperone for superoxide dismutase, chloroplastic/cytosolic

Gene

CCS

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Copper chaperone for the superoxide dismutases CSD1, CSD2 and CSD3. Binds copper ions and delivers them specifically to CSDs. Is required for assistance in CSDs disulfide bond formation and thereby activation of CSDs. May be involved in the negative regulation of heat stress-responsive genes and thermotolerance.4 Publications

Cofactori

Cu2+PROSITE-ProRule annotationNote: Binds 2 copper ions per subunit.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi97Copper 1PROSITE-ProRule annotation1
Metal bindingi100Copper 1PROSITE-ProRule annotation1
Metal bindingi300Copper 2PROSITE-ProRule annotation1
Metal bindingi302Copper 2PROSITE-ProRule annotation1

GO - Molecular functioni

  • superoxide dismutase copper chaperone activity Source: TAIR
  • zinc ion binding Source: GO_Central

GO - Biological processi

  • cellular copper ion homeostasis Source: TAIR
  • metal ion transport Source: InterPro
  • superoxide metabolic process Source: InterPro

Keywordsi

Molecular functionChaperone
Biological processStress response
LigandCopper, Metal-binding

Enzyme and pathway databases

ReactomeiR-ATH-3299685 Detoxification of Reactive Oxygen Species

Names & Taxonomyi

Protein namesi
Recommended name:
Copper chaperone for superoxide dismutase, chloroplastic/cytosolic
Short name:
AtCCS
Alternative name(s):
Superoxide dismutase copper chaperone
Gene namesi
Name:CCS
Ordered Locus Names:At1g12520
ORF Names:F5O11.26, T12C24.6
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G12520
TAIRilocus:2034740 AT1G12520

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Cytoplasm, Plastid

Pathology & Biotechi

Disruption phenotypei

No visible phenotype under normal growth conditions, but mutant plants have increased tolerance to heat stress.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 67ChloroplastSequence analysisAdd BLAST67
ChainiPRO_000042275768 – 320Copper chaperone for superoxide dismutase, chloroplastic/cytosolicAdd BLAST253

Proteomic databases

PaxDbiQ9LD47
PRIDEiQ9LD47

Expressioni

Tissue specificityi

Expressed in roots, shoots, stems and flowers, and at lower levels in rosette and cauline leaves.1 Publication

Inductioni

By copper and senescence. Down-regulated by heat stress.2 Publications

Gene expression databases

ExpressionAtlasiQ9LD47 baseline and differential
GenevisibleiQ9LD47 AT

Interactioni

Subunit structurei

Interacts with CSD1.1 Publication

Protein-protein interaction databases

BioGridi23048, 4 interactors
IntActiQ9LD47, 5 interactors
STRINGi3702.AT1G12520.1

Structurei

3D structure databases

ProteinModelPortaliQ9LD47
SMRiQ9LD47
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini87 – 150HMAPROSITE-ProRule annotationAdd BLAST64

Sequence similaritiesi

In the C-terminal section; belongs to the Cu-Zn superoxide dismutase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG4656 Eukaryota
COG2608 LUCA
HOGENOMiHOG000263450
InParanoidiQ9LD47
KOiK04569
OMAiGVTLWEE
OrthoDBiEOG09360JBV
PhylomeDBiQ9LD47

Family and domain databases

CDDicd00371 HMA, 1 hit
Gene3Di2.60.40.200, 1 hit
InterProiView protein in InterPro
IPR006121 HMA_dom
IPR036163 HMA_dom_sf
IPR036423 SOD-like_Cu/Zn_dom_sf
IPR024134 SOD_Cu/Zn_/chaperone
IPR001424 SOD_Cu_Zn_dom
PANTHERiPTHR10003 PTHR10003, 1 hit
PfamiView protein in Pfam
PF00403 HMA, 1 hit
PF00080 Sod_Cu, 1 hit
SUPFAMiSSF49329 SSF49329, 1 hit
SSF55008 SSF55008, 1 hit
PROSITEiView protein in PROSITE
PS50846 HMA_2, 1 hit

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9LD47-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASILRSVAT TSAVVAAASA IPIAIAFSSS SSSSSTNPKS QSLNFSFLSR
60 70 80 90 100
SSPRLLGLSR SFVSSPMATA LTSDRNLHQE DRAMPQLLTE FMVDMTCEGC
110 120 130 140 150
VNAVKNKLET IEGIEKVEVD LSNQVVRILG SSPVKAMTQA LEQTGRKARL
160 170 180 190 200
IGQGVPQDFL VSAAVAEFKG PDIFGVVRFA QVSMELARIE ANFTGLSPGT
210 220 230 240 250
HSWCINEYGD LTNGAASTGS LYNPFQDQTG TEPLGDLGTL EADKNGEAFY
260 270 280 290 300
SGKKEKLKVA DLIGRAVVVY KTDDNKSGPG LTAAVIARSA GVGENYKKLC
310 320
SCDGTVIWEA TNSDFVASKV
Length:320
Mass (Da):33,851
Last modified:October 1, 2000 - v1
Checksum:iFCD7A30742B91840
GO
Isoform 2 (identifier: Q9LD47-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-91: Missing.

Note: No experimental confirmation available.
Show »
Length:229
Mass (Da):24,319
Checksum:iD22CD688EA8D04D9
GO
Isoform 3 (identifier: Q9LD47-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-136: Missing.

Note: No experimental confirmation available.
Show »
Length:184
Mass (Da):19,489
Checksum:i1073BECE557618CE
GO

Sequence cautioni

The sequence AAK91374 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti239T → A in AAY22970 (PubMed:16126858).Curated1
Sequence conflicti313S → N in AAY22970 (PubMed:16126858).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0466121 – 136Missing in isoform 3. 1 PublicationAdd BLAST136
Alternative sequenceiVSP_0466131 – 91Missing in isoform 2. 1 PublicationAdd BLAST91

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ003058 mRNA Translation: AAY22970.1
AC025416 Genomic DNA Translation: AAF79650.1
AC025417 Genomic DNA Translation: AAF88100.1
CP002684 Genomic DNA Translation: AEE28891.1
CP002684 Genomic DNA Translation: AEE28892.1
CP002684 Genomic DNA Translation: AEE28893.1
AK175663 mRNA Translation: BAD43426.1
AK176660 mRNA Translation: BAD44423.1
AK221656 mRNA Translation: BAD95327.1
AY050357 mRNA Translation: AAK91374.1 Different initiation.
AY094034 mRNA Translation: AAM16190.1
AF179371 mRNA Translation: AAD52685.1
PIRiD86259
RefSeqiNP_001031029.1, NM_001035952.3 [Q9LD47-2]
NP_001031030.1, NM_001035953.2 [Q9LD47-3]
NP_563910.2, NM_101123.4 [Q9LD47-1]
UniGeneiAt.10574
At.75575

Genome annotation databases

EnsemblPlantsiAT1G12520.1; AT1G12520.1; AT1G12520 [Q9LD47-1]
AT1G12520.2; AT1G12520.2; AT1G12520 [Q9LD47-2]
AT1G12520.3; AT1G12520.3; AT1G12520 [Q9LD47-3]
GeneIDi837808
GrameneiAT1G12520.1; AT1G12520.1; AT1G12520 [Q9LD47-1]
AT1G12520.2; AT1G12520.2; AT1G12520 [Q9LD47-2]
AT1G12520.3; AT1G12520.3; AT1G12520 [Q9LD47-3]
KEGGiath:AT1G12520

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiCCS_ARATH
AccessioniPrimary (citable) accession number: Q9LD47
Secondary accession number(s): Q4ZJI5
, Q67Y08, Q681F4, Q94A55, Q9SPD6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 26, 2013
Last sequence update: October 1, 2000
Last modified: May 23, 2018
This is version 125 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health