Reviewed, UniProtKB/Swiss-Prot Q9LD46 (CRD1_CHLRE)

Names and origin

Protein names

Recommended name:
    Magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase 1, chloroplastic
      Short name=Mg-protoporphyrin IX monomethyl ester oxidative cyclase 1
    EC=1.14.13.81
Alternative name(s):
    Copper response defect 1 protein
    Copper-response target 1 protein

Gene names

Name:

CRD1

Organism

Chlamydomonas reinhardtii

Taxonomic identifier

3055 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Chlorophyta › Chlorophyceae › Chlamydomonadales › Chlamydomonadaceae › Chlamydomonas

Protein attributes

Sequence length	407 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the formation of the isocyclic ring in chlorophyll biosynthesis under oxygen- and copper-deficient conditions. Mediates the cyclase reaction, which results in the formation of divinylprotochlorophyllide (Pchlide) characteristic of all chlorophylls from magnesium-protoporphyrin IX 13-monomethyl ester (MgPMME). Ref.1 Ref.2
Catalytic activity	Magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O2 = 131-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADP+ + H2O. 131-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O2 = 131-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADP+ + 2 H2O. 131-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O2 = divinylprotochlorophyllide + NADP+ + 2 H2O.
Cofactor	Iron By similarity.
Pathway	Porphyrin biosynthesis; chlorophyll biosynthesis.
Subcellular location	Plastid › chloroplast thylakoid membrane. Ref.2 Ref.3
Induction	Induced in absence of copper and oxygen. Regulated by CRR1 protein, which activates its transcription in absence of copper. Ref.1 Ref.3 Ref.4
Sequence similarities	Belongs to the acsF family.

Ontologies

Keywords
Biological process	Chlorophyll biosynthesis Photosynthesis
Cellular component	Chloroplast Membrane Plastid Thylakoid
Domain	Transit peptide
Ligand	Iron Metal-binding NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	chlorophyll biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW photosynthesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast thylakoid membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Chloroplast Potential
	Chain	? – 407		Magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase 1, chloroplastic		PRO_0000000599

Sequences

	Sequence		Length	Mass (Da)
	Q9LD46-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: FD13EE5AC9C55CFE	FASTA	407	47,212
	10 20 30 40 50 60 MQTTLKQQRA SGRVSARQPF RSAAVARPRR STVRVQASAA PLNDGLGFET MRDGIKVAAK 70 80 90 100 110 120 ETLLTPRFYT TDFDEMEQLF SKEINPNLDM EELNACLNEF RNDYNKVHFV RNETFKAAAD 130 140 150 160 170 180 KVTGETRRIF IEFLERSCTA EFSGFLLYKE LARRMKASSP EVAEMFLLMS RDEARHAGFL 190 200 210 220 230 240 NKALSDFNLA LDLGFLTKNR TYTYFKPKFI IYATFLSEKI GYWRYITIYR HLQRNPDNQF 250 260 270 280 290 300 YPLFEYFENW CQDENRHGDF LAACLKAKPE LLNTFEAKLW SKFFCLSVYI TMYLNDHQRT 310 320 330 340 350 360 KFYESLGLNT RQFNQHVIIE TNRATERLFP VVPDVEDPRF FEILNKMVDV NAKLVELSAS 370 380 390 400 SSPLAGLQKL PLLERMASYC LQLLFFKEKD VGSVDIAGSG ASRNLAY

References

[1]	"The Crd1 gene encodes a putative di-iron enzyme required for photosystem I accumulation in copper deficiency and hypoxia in Chlamydomonas reinhardtii." Moseley J.L., Quinn J., Eriksson M., Merchant S. EMBO J. 19:2139-2151(2000) [PubMed: 10811605] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INDUCTION.
[2]	"Adaptation to Fe-deficiency requires remodeling of the photosynthetic apparatus." Moseley J.L., Allinger T., Herzog S., Hoerth P., Wehinger E., Merchant S., Hippler M. EMBO J. 21:6709-6720(2002) [PubMed: 12485992] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[3]	"Reciprocal expression of two candidate di-iron enzymes affecting photosystem I and light-harvesting complex accumulation." Moseley J.L., Page M.D., Alder N.P., Eriksson M., Quinn J., Soto F., Theg S.M., Hippler M., Merchant S. Plant Cell 14:673-688(2002) [PubMed: 11910013] [Abstract] Cited for: SUBCELLULAR LOCATION, INDUCTION.
[4]	"Oxygen deficiency responsive gene expression in Chlamydomonas reinhardtii through a copper-sensing signal transduction pathway." Quinn J.M., Eriksson M., Moseley J.L., Merchant S. Plant Physiol. 128:463-471(2002) [PubMed: 11842150] [Abstract] Cited for: INDUCTION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF226628 Genomic DNA. Translation: AAF65221.1. AF237671 mRNA. Translation: AAF63477.1.
RefSeq	XP_001692557.1.
UniGene	Cre.5891
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	Q9LD46.
Genome annotation databases
GeneID	5718021.
KEGG	cre:CHLREDRAFT_183476.
Enzyme and pathway databases
BRENDA	1.14.13.81. 144.
Family and domain databases
InterPro	IPR008434. AcsF. IPR003251. Rubrerythrin. [Graphical view]
Pfam	PF02915. Rubrerythrin. 1 hit. [Graphical view]
TIGRFAMs	TIGR02029. AcsF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CRD1_CHLRE

Accession

Primary (citable) accession number: Q9LD46

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2005
Last sequence update:	October 1, 2000
Last modified:	June 16, 2009
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)