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Protein

Ribosomal RNA small subunit methyltransferase G

Gene

rsmG

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Specifically methylates the N7 position of guanine in position 527 of 16S rRNA. Shows a marked preference for deproteinized 16S rRNA as substrate and is completely inactive with native 30S subunits as substrate.UniRule annotation1 Publication

Catalytic activityi

S-adenosyl-L-methionine + guanine(527) in 16S rRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(527) in 16S rRNA.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei88S-adenosyl-L-methionine; via carbonyl oxygen1
Binding sitei93S-adenosyl-L-methionine; via carbonyl oxygen1
Binding sitei158S-adenosyl-L-methionine1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

rRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BRENDAi2.1.1.170. 2305.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal RNA small subunit methyltransferase GUniRule annotation (EC:2.1.1.170UniRule annotation)
Alternative name(s):
16S rRNA 7-methylguanosine methyltransferaseUniRule annotation
Short name:
16S rRNA m7G methyltransferaseUniRule annotation
Glucose-inhibited division protein B
Gene namesi
Name:rsmGUniRule annotation
Synonyms:gidB
Ordered Locus Names:TTHA1971
OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifieri300852 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
Proteomesi
  • UP000000532 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Low-level streptomycin resistance.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001843581 – 249Ribosomal RNA small subunit methyltransferase GAdd BLAST249

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi164 ↔ 2491 Publication

Keywords - PTMi

Disulfide bond

Interactioni

Protein-protein interaction databases

STRINGi300852.TTHA1971.

Structurei

Secondary structure

1249
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi7 – 9Combined sources3
Helixi12 – 24Combined sources13
Helixi30 – 32Combined sources3
Helixi33 – 45Combined sources13
Helixi58 – 70Combined sources13
Helixi71 – 74Combined sources4
Beta strandi83 – 87Combined sources5
Turni90 – 94Combined sources5
Helixi95 – 101Combined sources7
Beta strandi106 – 112Combined sources7
Helixi114 – 127Combined sources14
Beta strandi130 – 136Combined sources7
Helixi139 – 142Combined sources4
Turni146 – 150Combined sources5
Beta strandi152 – 160Combined sources9
Helixi163 – 170Combined sources8
Helixi171 – 173Combined sources3
Beta strandi174 – 184Combined sources11
Helixi189 – 192Combined sources4
Helixi195 – 201Combined sources7
Beta strandi204 – 213Combined sources10
Turni215 – 217Combined sources3
Beta strandi220 – 228Combined sources9
Helixi242 – 245Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3G88X-ray1.87A/B1-249[»]
3G89X-ray1.50A/B1-249[»]
3G8AX-ray2.10A/B/C/D/E/F1-249[»]
3G8BX-ray2.10A/B1-249[»]
ProteinModelPortaliQ9LCY2.
SMRiQ9LCY2.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9LCY2.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni111 – 113S-adenosyl-L-methionine binding3
Regioni139 – 140S-adenosyl-L-methionine binding2
Regioni245 – 246RNA bindingSequence analysis2

Sequence similaritiesi

Belongs to the methyltransferase superfamily. RNA methyltransferase RsmG family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107YBR. Bacteria.
COG0357. LUCA.
HOGENOMiHOG000221014.
KOiK03501.
OMAiGVYLKHF.
PhylomeDBiQ9LCY2.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_00074. 16SrRNA_methyltr_G. 1 hit.
InterProiIPR003682. rRNA_ssu_MeTfrase_G.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF02527. GidB. 1 hit.
[Graphical view]
PIRSFiPIRSF003078. GidB. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00138. rsmG_gidB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9LCY2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFHGKHPGGL SERGRALLLE GGKALGLDLK PHLEAFSRLY ALLQEASGKV
60 70 80 90 100
NLTALRGEEE VVVKHFLDSL TLLRLPLWQG PLRVLDLGTG AGFPGLPLKI
110 120 130 140 150
VRPELELVLV DATRKKVAFV ERAIEVLGLK GARALWGRAE VLAREAGHRE
160 170 180 190 200
AYARAVARAV APLCVLSELL LPFLEVGGAA VAMKGPRVEE ELAPLPPALE
210 220 230 240
RLGGRLGEVL ALQLPLSGEA RHLVVLEKTA PTPPAYPRRP GVPERHPLC
Length:249
Mass (Da):26,832
Last modified:March 29, 2005 - v2
Checksum:iD346C3401D4A1619
GO

Sequence cautioni

The sequence CAB89206 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti198 – 221ALERL…SGEAR → LWSGSAGGLGRSSPSKLPSP GRRC in CAB89206 (PubMed:11167017).CuratedAdd BLAST24
Sequence conflicti229T → R in CAB89206 (PubMed:11167017).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ277593 Genomic DNA. Translation: CAB89206.1. Different initiation.
AP008226 Genomic DNA. Translation: BAD71794.1.
RefSeqiWP_011229055.1. NC_006461.1.
YP_145237.1. NC_006461.1.

Genome annotation databases

EnsemblBacteriaiBAD71794; BAD71794; BAD71794.
GeneIDi3169844.
KEGGittj:TTHA1971.
PATRICi23958921. VBITheThe93045_1942.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ277593 Genomic DNA. Translation: CAB89206.1. Different initiation.
AP008226 Genomic DNA. Translation: BAD71794.1.
RefSeqiWP_011229055.1. NC_006461.1.
YP_145237.1. NC_006461.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3G88X-ray1.87A/B1-249[»]
3G89X-ray1.50A/B1-249[»]
3G8AX-ray2.10A/B/C/D/E/F1-249[»]
3G8BX-ray2.10A/B1-249[»]
ProteinModelPortaliQ9LCY2.
SMRiQ9LCY2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi300852.TTHA1971.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD71794; BAD71794; BAD71794.
GeneIDi3169844.
KEGGittj:TTHA1971.
PATRICi23958921. VBITheThe93045_1942.

Phylogenomic databases

eggNOGiENOG4107YBR. Bacteria.
COG0357. LUCA.
HOGENOMiHOG000221014.
KOiK03501.
OMAiGVYLKHF.
PhylomeDBiQ9LCY2.

Enzyme and pathway databases

BRENDAi2.1.1.170. 2305.

Miscellaneous databases

EvolutionaryTraceiQ9LCY2.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_00074. 16SrRNA_methyltr_G. 1 hit.
InterProiIPR003682. rRNA_ssu_MeTfrase_G.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF02527. GidB. 1 hit.
[Graphical view]
PIRSFiPIRSF003078. GidB. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00138. rsmG_gidB. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRSMG_THET8
AccessioniPrimary (citable) accession number: Q9LCY2
Secondary accession number(s): Q5SGV9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 16, 2002
Last sequence update: March 29, 2005
Last modified: November 2, 2016
This is version 91 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.