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Protein

Ribosomal RNA small subunit methyltransferase G

Gene

rsmG

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Specifically methylates the N7 position of guanine in position 527 of 16S rRNA. Shows a marked preference for deproteinized 16S rRNA as substrate and is completely inactive with native 30S subunits as substrate.UniRule annotation1 Publication

Catalytic activityi

S-adenosyl-L-methionine + guanine(527) in 16S rRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(527) in 16S rRNA.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei88 – 881S-adenosyl-L-methionine; via carbonyl oxygen
Binding sitei93 – 931S-adenosyl-L-methionine; via carbonyl oxygen
Binding sitei158 – 1581S-adenosyl-L-methionine

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

rRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciTTHE300852:GH8R-2021-MONOMER.
BRENDAi2.1.1.170. 2305.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal RNA small subunit methyltransferase GUniRule annotation (EC:2.1.1.170UniRule annotation)
Alternative name(s):
16S rRNA 7-methylguanosine methyltransferaseUniRule annotation
Short name:
16S rRNA m7G methyltransferaseUniRule annotation
Glucose-inhibited division protein B
Gene namesi
Name:rsmGUniRule annotation
Synonyms:gidB
Ordered Locus Names:TTHA1971
OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifieri300852 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
Proteomesi
  • UP000000532 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Low-level streptomycin resistance.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 249249Ribosomal RNA small subunit methyltransferase GPRO_0000184358Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi164 ↔ 2491 Publication

Keywords - PTMi

Disulfide bond

Interactioni

Protein-protein interaction databases

STRINGi300852.TTHA1971.

Structurei

Secondary structure

1
249
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi7 – 93Combined sources
Helixi12 – 2413Combined sources
Helixi30 – 323Combined sources
Helixi33 – 4513Combined sources
Helixi58 – 7013Combined sources
Helixi71 – 744Combined sources
Beta strandi83 – 875Combined sources
Turni90 – 945Combined sources
Helixi95 – 1017Combined sources
Beta strandi106 – 1127Combined sources
Helixi114 – 12714Combined sources
Beta strandi130 – 1367Combined sources
Helixi139 – 1424Combined sources
Turni146 – 1505Combined sources
Beta strandi152 – 1609Combined sources
Helixi163 – 1708Combined sources
Helixi171 – 1733Combined sources
Beta strandi174 – 18411Combined sources
Helixi189 – 1924Combined sources
Helixi195 – 2017Combined sources
Beta strandi204 – 21310Combined sources
Turni215 – 2173Combined sources
Beta strandi220 – 2289Combined sources
Helixi242 – 2454Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3G88X-ray1.87A/B1-249[»]
3G89X-ray1.50A/B1-249[»]
3G8AX-ray2.10A/B/C/D/E/F1-249[»]
3G8BX-ray2.10A/B1-249[»]
ProteinModelPortaliQ9LCY2.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9LCY2.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni111 – 1133S-adenosyl-L-methionine binding
Regioni139 – 1402S-adenosyl-L-methionine binding
Regioni245 – 2462RNA bindingSequence analysis

Sequence similaritiesi

Belongs to the methyltransferase superfamily. RNA methyltransferase RsmG family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107YBR. Bacteria.
COG0357. LUCA.
HOGENOMiHOG000221014.
KOiK03501.
OMAiGVYLKHF.
OrthoDBiEOG6HF639.
PhylomeDBiQ9LCY2.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_00074. 16SrRNA_methyltr_G.
InterProiIPR003682. rRNA_ssu_MeTfrase_G.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF02527. GidB. 1 hit.
[Graphical view]
PIRSFiPIRSF003078. GidB. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00138. rsmG_gidB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9LCY2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFHGKHPGGL SERGRALLLE GGKALGLDLK PHLEAFSRLY ALLQEASGKV
60 70 80 90 100
NLTALRGEEE VVVKHFLDSL TLLRLPLWQG PLRVLDLGTG AGFPGLPLKI
110 120 130 140 150
VRPELELVLV DATRKKVAFV ERAIEVLGLK GARALWGRAE VLAREAGHRE
160 170 180 190 200
AYARAVARAV APLCVLSELL LPFLEVGGAA VAMKGPRVEE ELAPLPPALE
210 220 230 240
RLGGRLGEVL ALQLPLSGEA RHLVVLEKTA PTPPAYPRRP GVPERHPLC
Length:249
Mass (Da):26,832
Last modified:March 29, 2005 - v2
Checksum:iD346C3401D4A1619
GO

Sequence cautioni

The sequence CAB89206.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti198 – 22124ALERL…SGEAR → LWSGSAGGLGRSSPSKLPSP GRRC in CAB89206 (PubMed:11167017).CuratedAdd
BLAST
Sequence conflicti229 – 2291T → R in CAB89206 (PubMed:11167017).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ277593 Genomic DNA. Translation: CAB89206.1. Different initiation.
AP008226 Genomic DNA. Translation: BAD71794.1.
RefSeqiWP_011229055.1. NC_006461.1.
YP_145237.1. NC_006461.1.

Genome annotation databases

EnsemblBacteriaiBAD71794; BAD71794; BAD71794.
GeneIDi3169844.
KEGGittj:TTHA1971.
PATRICi23958921. VBITheThe93045_1942.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ277593 Genomic DNA. Translation: CAB89206.1. Different initiation.
AP008226 Genomic DNA. Translation: BAD71794.1.
RefSeqiWP_011229055.1. NC_006461.1.
YP_145237.1. NC_006461.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3G88X-ray1.87A/B1-249[»]
3G89X-ray1.50A/B1-249[»]
3G8AX-ray2.10A/B/C/D/E/F1-249[»]
3G8BX-ray2.10A/B1-249[»]
ProteinModelPortaliQ9LCY2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi300852.TTHA1971.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD71794; BAD71794; BAD71794.
GeneIDi3169844.
KEGGittj:TTHA1971.
PATRICi23958921. VBITheThe93045_1942.

Phylogenomic databases

eggNOGiENOG4107YBR. Bacteria.
COG0357. LUCA.
HOGENOMiHOG000221014.
KOiK03501.
OMAiGVYLKHF.
OrthoDBiEOG6HF639.
PhylomeDBiQ9LCY2.

Enzyme and pathway databases

BioCyciTTHE300852:GH8R-2021-MONOMER.
BRENDAi2.1.1.170. 2305.

Miscellaneous databases

EvolutionaryTraceiQ9LCY2.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_00074. 16SrRNA_methyltr_G.
InterProiIPR003682. rRNA_ssu_MeTfrase_G.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF02527. GidB. 1 hit.
[Graphical view]
PIRSFiPIRSF003078. GidB. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00138. rsmG_gidB. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and characterization of the dnaA upstream region of Thermus thermophilus."
    Nardmann J., Messer W.
    Gene 261:299-303(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: HB8 / ATCC 27634 / DSM 579.
  2. "Complete genome sequence of Thermus thermophilus HB8."
    Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.
    Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: HB8 / ATCC 27634 / DSM 579.
  3. "Structural and functional studies of the Thermus thermophilus 16S rRNA methyltransferase RsmG."
    Gregory S.T., Demirci H., Belardinelli R., Monshupanee T., Gualerzi C., Dahlberg A.E., Jogl G.
    RNA 15:1693-1704(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.50 ANGSTROMS) IN COMPLEXES WITH AMP; S-ADENOSYL-L-HOMOCYSTEINE AND S-ADENOSYL-L-METHIONINE, FUNCTION, DISRUPTION PHENOTYPE, DISULFIDE BOND.
    Strain: HB8 / ATCC 27634 / DSM 579.

Entry informationi

Entry nameiRSMG_THET8
AccessioniPrimary (citable) accession number: Q9LCY2
Secondary accession number(s): Q5SGV9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 16, 2002
Last sequence update: March 29, 2005
Last modified: November 11, 2015
This is version 89 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.