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Protein

Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B

Gene

gatB

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln).

Catalytic activityi

ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate.UniRule annotation
ATP + L-aspartyl-tRNA(Asn) + L-glutamine = ADP + phosphate + L-asparaginyl-tRNA(Asn) + L-glutamate.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciTTHE300852:GH8R-384-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit BUniRule annotation (EC:6.3.5.-UniRule annotation)
Short name:
Asp/Glu-ADT subunit BUniRule annotation
Gene namesi
Name:gatBUniRule annotation
Ordered Locus Names:TTHA0366
OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifieri300852 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
Proteomesi
  • UP000000532 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 469469Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit BPRO_0000148858Add
BLAST

Interactioni

Subunit structurei

Heterotrimer of A, B and C subunits.UniRule annotation

Protein-protein interaction databases

STRINGi300852.TTHA0366.

Structurei

Secondary structure

1
469
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi4 – 1411Combined sources
Beta strandi20 – 223Combined sources
Turni39 – 435Combined sources
Helixi53 – 6614Combined sources
Beta strandi72 – 743Combined sources
Beta strandi77 – 804Combined sources
Beta strandi89 – 935Combined sources
Beta strandi95 – 973Combined sources
Beta strandi99 – 1024Combined sources
Beta strandi104 – 1074Combined sources
Beta strandi110 – 12112Combined sources
Beta strandi125 – 1295Combined sources
Beta strandi132 – 1365Combined sources
Beta strandi143 – 1497Combined sources
Helixi156 – 17318Combined sources
Helixi180 – 1823Combined sources
Beta strandi185 – 1939Combined sources
Beta strandi196 – 1994Combined sources
Beta strandi204 – 2096Combined sources
Helixi212 – 23019Combined sources
Turni245 – 2484Combined sources
Helixi277 – 2848Combined sources
Helixi291 – 2988Combined sources
Turni299 – 3024Combined sources
Helixi305 – 3128Combined sources
Helixi315 – 32410Combined sources
Beta strandi327 – 3304Combined sources
Helixi332 – 3409Combined sources
Helixi342 – 3509Combined sources
Helixi361 – 37212Combined sources
Helixi378 – 38811Combined sources
Turni389 – 3913Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3KFUX-ray3.00F/I1-396[»]
ProteinModelPortaliQ9LCX2.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9LCX2.

Family & Domainsi

Sequence similaritiesi

Belongs to the GatB/GatE family. GatB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CHT. Bacteria.
COG0064. LUCA.
HOGENOMiHOG000223742.
KOiK02434.
OMAiESADYRY.
OrthoDBiEOG6RJV5B.
PhylomeDBiQ9LCX2.

Family and domain databases

HAMAPiMF_00121. GatB.
InterProiIPR004413. Apn/Gln-ADT_bsu.
IPR017959. Asn/Gln-tRNA_amidoTrfase_suB/E.
IPR006075. Asn/Gln-tRNA_Trfase_suB/E_cat.
IPR018027. Asn/Gln_amidotransferase.
IPR003789. Asn/Gln_tRNA_amidoTrfrase-rel.
IPR017958. Gln-tRNA_amidoTrfase_suB_CS.
[Graphical view]
PANTHERiPTHR11659. PTHR11659. 1 hit.
PfamiPF02934. GatB_N. 1 hit.
PF02637. GatB_Yqey. 1 hit.
[Graphical view]
SMARTiSM00845. GatB_Yqey. 1 hit.
[Graphical view]
SUPFAMiSSF89095. SSF89095. 1 hit.
TIGRFAMsiTIGR00133. gatB. 1 hit.
PROSITEiPS01234. GATB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9LCX2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYEAVIGLEV HLHLKTRTKM FCGCRADYFG AEPNTHTCPV CLGLPGALPV
60 70 80 90 100
PNRVAVEHGL RLALALGAEV PERLVFHRKN YFYPDLPKNY QISQYDLPLG
110 120 130 140 150
RGGSLPLGER RVRIKRLHLE EDAGKSLHLE GRTLLDLNRA GSPLIELVTE
160 170 180 190 200
PDLKTPEEAR LFLQRIQALV QTLGISDASP EEGKLRADVN VSVRRVGEPL
210 220 230 240 250
GTKVEIKNLN SFKSVQRALE YEIRRQTEIL RRGEKVKQAT MGFEEGSGKT
260 270 280 290 300
YPMRTKEEEA DYRYFPEPDL PPVAIPRDWL EEVRRSLPEL PWEKEARYRA
310 320 330 340 350
LGIKEKDAEV LAYTPSLARF LDQALPLGLA SPQALANWLL ADVAGLLHER
360 370 380 390 400
GLRLEETRLS PEGLARLVGL FERGEVTSRV AKSLLPEVLE GQDPEALVRE
410 420 430 440 450
RGLKVVADEG ALKALVAEAI AAMPEAAESV RQGKVKALDA LVGQVMRKTR
460
GQARPDLVRR LLLEALGVG
Length:469
Mass (Da):52,236
Last modified:October 1, 2000 - v1
Checksum:i10D01DFF9C9B0625
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF202448 Genomic DNA. Translation: AAF91177.1.
AP008226 Genomic DNA. Translation: BAD70189.1.
RefSeqiWP_011227883.1. NC_006461.1.
YP_143632.1. NC_006461.1.

Genome annotation databases

EnsemblBacteriaiBAD70189; BAD70189; BAD70189.
GeneIDi3169083.
KEGGittj:TTHA0366.
PATRICi23955696. VBITheThe93045_0366.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF202448 Genomic DNA. Translation: AAF91177.1.
AP008226 Genomic DNA. Translation: BAD70189.1.
RefSeqiWP_011227883.1. NC_006461.1.
YP_143632.1. NC_006461.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3KFUX-ray3.00F/I1-396[»]
ProteinModelPortaliQ9LCX2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi300852.TTHA0366.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD70189; BAD70189; BAD70189.
GeneIDi3169083.
KEGGittj:TTHA0366.
PATRICi23955696. VBITheThe93045_0366.

Phylogenomic databases

eggNOGiENOG4105CHT. Bacteria.
COG0064. LUCA.
HOGENOMiHOG000223742.
KOiK02434.
OMAiESADYRY.
OrthoDBiEOG6RJV5B.
PhylomeDBiQ9LCX2.

Enzyme and pathway databases

BioCyciTTHE300852:GH8R-384-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ9LCX2.

Family and domain databases

HAMAPiMF_00121. GatB.
InterProiIPR004413. Apn/Gln-ADT_bsu.
IPR017959. Asn/Gln-tRNA_amidoTrfase_suB/E.
IPR006075. Asn/Gln-tRNA_Trfase_suB/E_cat.
IPR018027. Asn/Gln_amidotransferase.
IPR003789. Asn/Gln_tRNA_amidoTrfrase-rel.
IPR017958. Gln-tRNA_amidoTrfase_suB_CS.
[Graphical view]
PANTHERiPTHR11659. PTHR11659. 1 hit.
PfamiPF02934. GatB_N. 1 hit.
PF02637. GatB_Yqey. 1 hit.
[Graphical view]
SMARTiSM00845. GatB_Yqey. 1 hit.
[Graphical view]
SUPFAMiSSF89095. SSF89095. 1 hit.
TIGRFAMsiTIGR00133. gatB. 1 hit.
PROSITEiPS01234. GATB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The heterotrimeric Thermus thermophilus Asp-tRNA(Asn) amidotransferase can also generate Gln-tRNA(Gln)."
    Becker H.D., Min B., Jacobi C., Raczniak G., Pelaschier J., Roy H., Klein S., Kern D., Soell D.
    FEBS Lett. 476:140-144(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
  2. "Complete genome sequence of Thermus thermophilus HB8."
    Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.
    Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: HB8 / ATCC 27634 / DSM 579.

Entry informationi

Entry nameiGATB_THET8
AccessioniPrimary (citable) accession number: Q9LCX2
Secondary accession number(s): Q5SLC7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 2, 2002
Last sequence update: October 1, 2000
Last modified: November 11, 2015
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.