Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B

Gene

gatB

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln).

Catalytic activityi

ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate.UniRule annotation
ATP + L-aspartyl-tRNA(Asn) + L-glutamine = ADP + phosphate + L-asparaginyl-tRNA(Asn) + L-glutamate.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit BUniRule annotation (EC:6.3.5.-UniRule annotation)
Short name:
Asp/Glu-ADT subunit BUniRule annotation
Gene namesi
Name:gatBUniRule annotation
Ordered Locus Names:TTHA0366
OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifieri300852 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
Proteomesi
  • UP000000532 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001488581 – 469Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit BAdd BLAST469

Proteomic databases

PRIDEiQ9LCX2.

Interactioni

Subunit structurei

Heterotrimer of A, B and C subunits.UniRule annotation

Protein-protein interaction databases

STRINGi300852.TTHA0366.

Structurei

Secondary structure

1469
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 14Combined sources11
Beta strandi20 – 22Combined sources3
Turni39 – 43Combined sources5
Helixi53 – 66Combined sources14
Beta strandi72 – 74Combined sources3
Beta strandi77 – 80Combined sources4
Beta strandi89 – 93Combined sources5
Beta strandi95 – 97Combined sources3
Beta strandi99 – 102Combined sources4
Beta strandi104 – 107Combined sources4
Beta strandi110 – 121Combined sources12
Beta strandi125 – 129Combined sources5
Beta strandi132 – 136Combined sources5
Beta strandi143 – 149Combined sources7
Helixi156 – 173Combined sources18
Helixi180 – 182Combined sources3
Beta strandi185 – 193Combined sources9
Beta strandi196 – 199Combined sources4
Beta strandi204 – 209Combined sources6
Helixi212 – 230Combined sources19
Turni245 – 248Combined sources4
Helixi277 – 284Combined sources8
Helixi291 – 298Combined sources8
Turni299 – 302Combined sources4
Helixi305 – 312Combined sources8
Helixi315 – 324Combined sources10
Beta strandi327 – 330Combined sources4
Helixi332 – 340Combined sources9
Helixi342 – 350Combined sources9
Helixi361 – 372Combined sources12
Helixi378 – 388Combined sources11
Turni389 – 391Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3KFUX-ray3.00F/I1-396[»]
ProteinModelPortaliQ9LCX2.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9LCX2.

Family & Domainsi

Sequence similaritiesi

Belongs to the GatB/GatE family. GatB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CHT. Bacteria.
COG0064. LUCA.
HOGENOMiHOG000223742.
KOiK02434.
OMAiESADYRY.
PhylomeDBiQ9LCX2.

Family and domain databases

HAMAPiMF_00121. GatB. 1 hit.
InterProiIPR004413. Apn/Gln-ADT_bsu.
IPR017959. Asn/Gln-tRNA_amidoTrfase_suB/E.
IPR006075. Asn/Gln-tRNA_Trfase_suB/E_cat.
IPR018027. Asn/Gln_amidotransferase.
IPR003789. Asn/Gln_tRNA_amidoTrfrase-rel.
IPR017958. Gln-tRNA_amidoTrfase_suB_CS.
[Graphical view]
PANTHERiPTHR11659. PTHR11659. 1 hit.
PfamiPF02934. GatB_N. 1 hit.
PF02637. GatB_Yqey. 1 hit.
[Graphical view]
SMARTiSM00845. GatB_Yqey. 1 hit.
[Graphical view]
SUPFAMiSSF89095. SSF89095. 1 hit.
TIGRFAMsiTIGR00133. gatB. 1 hit.
PROSITEiPS01234. GATB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9LCX2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYEAVIGLEV HLHLKTRTKM FCGCRADYFG AEPNTHTCPV CLGLPGALPV
60 70 80 90 100
PNRVAVEHGL RLALALGAEV PERLVFHRKN YFYPDLPKNY QISQYDLPLG
110 120 130 140 150
RGGSLPLGER RVRIKRLHLE EDAGKSLHLE GRTLLDLNRA GSPLIELVTE
160 170 180 190 200
PDLKTPEEAR LFLQRIQALV QTLGISDASP EEGKLRADVN VSVRRVGEPL
210 220 230 240 250
GTKVEIKNLN SFKSVQRALE YEIRRQTEIL RRGEKVKQAT MGFEEGSGKT
260 270 280 290 300
YPMRTKEEEA DYRYFPEPDL PPVAIPRDWL EEVRRSLPEL PWEKEARYRA
310 320 330 340 350
LGIKEKDAEV LAYTPSLARF LDQALPLGLA SPQALANWLL ADVAGLLHER
360 370 380 390 400
GLRLEETRLS PEGLARLVGL FERGEVTSRV AKSLLPEVLE GQDPEALVRE
410 420 430 440 450
RGLKVVADEG ALKALVAEAI AAMPEAAESV RQGKVKALDA LVGQVMRKTR
460
GQARPDLVRR LLLEALGVG
Length:469
Mass (Da):52,236
Last modified:October 1, 2000 - v1
Checksum:i10D01DFF9C9B0625
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF202448 Genomic DNA. Translation: AAF91177.1.
AP008226 Genomic DNA. Translation: BAD70189.1.
RefSeqiWP_011227883.1. NC_006461.1.
YP_143632.1. NC_006461.1.

Genome annotation databases

EnsemblBacteriaiBAD70189; BAD70189; BAD70189.
GeneIDi3169083.
KEGGittj:TTHA0366.
PATRICi23955696. VBITheThe93045_0366.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF202448 Genomic DNA. Translation: AAF91177.1.
AP008226 Genomic DNA. Translation: BAD70189.1.
RefSeqiWP_011227883.1. NC_006461.1.
YP_143632.1. NC_006461.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3KFUX-ray3.00F/I1-396[»]
ProteinModelPortaliQ9LCX2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi300852.TTHA0366.

Proteomic databases

PRIDEiQ9LCX2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD70189; BAD70189; BAD70189.
GeneIDi3169083.
KEGGittj:TTHA0366.
PATRICi23955696. VBITheThe93045_0366.

Phylogenomic databases

eggNOGiENOG4105CHT. Bacteria.
COG0064. LUCA.
HOGENOMiHOG000223742.
KOiK02434.
OMAiESADYRY.
PhylomeDBiQ9LCX2.

Miscellaneous databases

EvolutionaryTraceiQ9LCX2.

Family and domain databases

HAMAPiMF_00121. GatB. 1 hit.
InterProiIPR004413. Apn/Gln-ADT_bsu.
IPR017959. Asn/Gln-tRNA_amidoTrfase_suB/E.
IPR006075. Asn/Gln-tRNA_Trfase_suB/E_cat.
IPR018027. Asn/Gln_amidotransferase.
IPR003789. Asn/Gln_tRNA_amidoTrfrase-rel.
IPR017958. Gln-tRNA_amidoTrfase_suB_CS.
[Graphical view]
PANTHERiPTHR11659. PTHR11659. 1 hit.
PfamiPF02934. GatB_N. 1 hit.
PF02637. GatB_Yqey. 1 hit.
[Graphical view]
SMARTiSM00845. GatB_Yqey. 1 hit.
[Graphical view]
SUPFAMiSSF89095. SSF89095. 1 hit.
TIGRFAMsiTIGR00133. gatB. 1 hit.
PROSITEiPS01234. GATB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGATB_THET8
AccessioniPrimary (citable) accession number: Q9LCX2
Secondary accession number(s): Q5SLC7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 2, 2002
Last sequence update: October 1, 2000
Last modified: November 2, 2016
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.