Q9LCV9 (Q9LCV9_STRCL) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names	Submitted name: Carboxyethylarginine synthase EMBL AAF86619.1
Organism	Streptomyces clavuligerus EMBL AAF86619.1
Taxonomic identifier	1901 [NCBI]
Taxonomic lineage	Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	573 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Sequence similarities

Belongs to the TPP enzyme family. RuleBase RU003702

Ontologies

Keywords
Ligand	Magnesium PDB 1UPB PDB 1UPA PDB 1UPC PDB 2IHT PDB 2IHV PDB 2IHU Metal-binding PDB 1UPB PDB 1UPA PDB 1UPC PDB 2IHT PDB 2IHV PDB 2IHU Thiamine pyrophosphate RuleBase RU004476 PDB 1UPB PDB 1UPA PDB 1UPC PDB 2IHT PDB 2IHV
Technical term	3D-structure PDB 1UPB PDB 1UPA PDB 1UPC PDB 2IHT PDB 2IHV PDB 2IHU
Gene Ontology (GO)
Molecular function	magnesium ion binding Inferred from electronic annotation. Source: InterPro thiamine pyrophosphate binding Inferred from electronic annotation. Source: InterPro transferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Region	410 – 413	4	Thiamine pyrophosphate binding PDB 1UPB PDB 1UPA PDB 1UPC PDB 2IHT PDB 2IHV
	Region	436 – 438	3	Thiamine pyrophosphate binding PDB 1UPB PDB 1UPA PDB 1UPC PDB 2IHT PDB 2IHV
	Region	463 – 465	3	Thiamine pyrophosphate binding PDB 1UPB PDB 1UPA PDB 1UPC PDB 2IHT PDB 2IHV
	Region	490 – 495	6	Thiamine pyrophosphate binding PDB 1UPB PDB 1UPA PDB 1UPC PDB 2IHT PDB 2IHV
Sites
	Metal binding	463	1	Magnesium PDB 1UPB PDB 1UPA PDB 1UPC PDB 2IHT PDB 2IHV PDB 2IHU
	Metal binding	490	1	Magnesium PDB 1UPB PDB 1UPA PDB 1UPC PDB 2IHT PDB 2IHV PDB 2IHU
	Metal binding	492	1	Magnesium; via carbonyl oxygen PDB 1UPB PDB 1UPA PDB 1UPC PDB 2IHT PDB 2IHV PDB 2IHU
	Binding site	57	1	Thiamine pyrophosphate PDB 1UPB PDB 1UPA PDB 1UPC PDB 2IHT PDB 2IHV
	Binding site	561	1	Thiamine pyrophosphate PDB 1UPB PDB 1UPA PDB 1UPC PDB 2IHT PDB 2IHV

Sequences

	Sequence		Length	Mass (Da)
	Q9LCV9 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 87624F6C0281C714	FASTA	573	60,907
	10 20 30 40 50 60 MSRVSTAPSG KPTAAHALLS RLRDHGVGKV FGVVGREAAS ILFDEVEGID FVLTRHEFTA 70 80 90 100 110 120 GVAADVLARI TGRPQACWAT LGPGMTNLST GIATSVLDRS PVIALAAQSE SHDIFPNDTH 130 140 150 160 170 180 QCLDSVAIVA PMSKYAVELQ RPHEITDLVD SAVNAAMTEP VGPSFISLPV DLLGSSEGID 190 200 210 220 230 240 TTVPNPPANT PAKPVGVVAD GWQKAADQAA ALLAEAKHPV LVVGAAAIRS GAVPAIRALA 250 260 270 280 290 300 ERLNIPVITT YIAKGVLPVG HELNYGAVTG YMDGILNFPA LQTMFAPVDL VLTVGYDYAE 310 320 330 340 350 360 DLRPSMWQKG IEKKTVRISP TVNPIPRVYR PDVDVVTDVL AFVEHFETAT ASFGAKQRHD 370 380 390 400 410 420 IEPLRARIAE FLADPETYED GMRVHQVIDS MNTVMEEAAE PGEGTIVSDI GFFRHYGVLF 430 440 450 460 470 480 ARADQPFGFL TSAGCSSFGY GIPAAIGAQM ARPDQPTFLI AGDGGFHSNS SDLETIARLN 490 500 510 520 530 540 LPIVTVVVNN DTNGLIELYQ NIGHHRSHDP AVKFGGVDFV ALAEANGVDA TRATNREELL 550 560 570 AALRKGAELG RPFLIEVPVN YDFQPGGFGA LSI

References

[1]	"A pathway-specific transcriptional activator regulates late steps of clavulanic acid biosynthesis in Streptomyces clavuligerus." Paradkar A.S., Aidoo K.A., Jensen S.E. Mol. Microbiol. 27:831-843(1998) [PubMed: 9515708] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: NRRL3585 EMBL AAF86619.1.
[2]	"Cloning, sequencing and disruption of a gene from Streptomyces clavuligerus involved in clavulanic acid biosynthesis." Aidoo K.A., Wong A., Alexander D.C., Rittammer R.A.R., Jensen S.E. Gene 147:41-46(1994) [PubMed: 8088547] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: NRRL3585 EMBL AAF86619.1.
[3]	"Molecular analysis of a beta-lactam resistance gene encoded within the cephamycin gene cluster of Streptomyces clavuligerus." Paradkar A.S., Aidoo K.A., Wong A., Jensen S.E. J. Bacteriol. 178:6266-6274(1996) [PubMed: 8892828] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: NRRL3585 EMBL AAF86619.1.
[4]	"Enzymes catalyzing the early steps of clavulanic acid biosynthesis are encoded by two sets of paralogous genes in Streptomyces clavuligerus." Jensen S.E., Elder K.J., Aidoo K.A., Paradkar A.S. Antimicrob. Agents Chemother. 44:720-726(2000) [PubMed: 10681345] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: NRRL3585 EMBL AAF86619.1.
[5]	"Crystal structure and mechanistic implications of N2-(2-carboxyethyl)arginine synthase, the first enzyme in the clavulanic acid biosynthesis pathway." Caines M.E., Elkins J.M., Hewitson K.S., Schofield C.J. J. Biol. Chem. 279:5685-5692(2004) [PubMed: 14623876] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.35 ANGSTROMS) IN COMPLEX WITH MAGNESIUM AND THIAMINE PYROPHOSPHATE.
[6]	"Structural and mechanistic studies on N(2)-(2-carboxyethyl)arginine synthase." Caines M.E., Sorensen J.L., Schofield C.J. Biochem. Biophys. Res. Commun. 385:512-517(2009) [PubMed: 19477162] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) IN COMPLEX WITH MAGNESIUM AND THIAMINE PYROPHOSPHATE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U87786 Genomic DNA. Translation: AAF86619.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1UPA	X-ray	2.35	A/B/C/D	1-573	[»]
1UPB	X-ray	2.35	A/B/C/D	1-573	[»]
1UPC	X-ray	2.45	A/B/C/D/E/F	1-573	[»]
2IHT	X-ray	2.00	A/B/C/D	1-573	[»]
2IHU	X-ray	2.05	A/B/C/D	1-573	[»]
2IHV	X-ray	2.30	A/B/C/D	1-573	[»]

ProteinModelPortal

Q9LCV9.

SMR

Q9LCV9. Positions 11-573.

ModBase

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Protocols and materials databases

StructuralBiologyKnowledgebase

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Genome annotation databases

PATRIC

29741625. VBIStrCla15562_0195.

Family and domain databases

InterPro

IPR012000. Thiamin_PyroP_enz_cen_dom.
IPR012001. Thiamin_PyroP_enz_TPP-bd_dom.
IPR000399. TPP-bd_CS.
IPR011766. TPP_enzyme-bd_C.
[Graphical view]

Pfam

PF02775. TPP_enzyme_C. 1 hit.
PF00205. TPP_enzyme_M. 1 hit.
PF02776. TPP_enzyme_N. 1 hit.
[Graphical view]

PROSITE

PS00187. TPP_ENZYMES. 1 hit.
[Graphical view]

ProtoNet

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Entry information

Entry name

Q9LCV9_STRCL

Accession

Primary (citable) accession number: Q9LCV9

Entry history

Integrated into UniProtKB/TrEMBL:	October 1, 2000
Last sequence update:	October 1, 2000
Last modified:	December 14, 2011
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)