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Q9LCT6 (DDLB_PSEAE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 87. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase B

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase B
D-alanylalanine synthetase B
Gene names
Name:ddlB
Ordered Locus Names:PA4410
OrganismPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) [Reference proteome] [HAMAP]
Taxonomic identifier208964 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Protein attributes

Sequence length319 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 319319D-alanine--D-alanine ligase B HAMAP-Rule MF_00047
PRO_0000177856

Regions

Domain117 – 312196ATP-grasp
Nucleotide binding143 – 19856ATP By similarity

Sites

Metal binding2661Magnesium or manganese 1 By similarity
Metal binding2791Magnesium or manganese 1 By similarity
Metal binding2791Magnesium or manganese 2 By similarity
Metal binding2811Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9LCT6 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 9470EF555A745419

FASTA31934,363
        10         20         30         40         50         60 
MNLCLDSLLN GTQDPKAFGR VAVLFGGKSA EREVSLKSGA MVLQSLLAAG VDAFGIDVGE 

        70         80         90        100        110        120 
DLLQRLVEEK IDRAFIILHG RGGEDGSMQG LLECAGIPYT GSGVLASALA MDKLRTKRVW 

       130        140        150        160        170        180 
LSLGLPTPDY AVLASEDDCR EAAQRLGFPL IVKPAHEGSS IGMAKVGGLD ELIAAWREAA 

       190        200        210        220        230        240 
RYDSQVLVEQ WISGPEFTVA TLRGQVLPAI RLGTPHTFYD YDAKYLASDT RYQVPCGLDE 

       250        260        270        280        290        300 
AKERELKELT ARACDALGIQ GWGRADVMQD AEGRFWLLEV NTAPGMTDHS LVPMAARAAG 

       310 
LDFQQLVLAI LADSREARG 

« Hide

References

« Hide 'large scale' references
[1]"Physical mapping of 38 loci including aimE, ampC, ampR, arcA, aroK, catR, cypH, dapB, envA, envC, ftsA, ftsZ, groEL, murE, opdE, oprD, oprF, oprH, oprI, oprK, oprP, pbpB, pbpC, pheS, phoA, phoB, phoS, ponA, pyoS1, qin, rpoB, rpoH, sodB, soxR, sucC."
Levesque R.C., Liao X., Lightfoot J., Charlebois I., Ouellet C., Morency M., Dewar K., Siehnel R., Lam J., Hancock R.E.
Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
[2]"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen."
Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. expand/collapse author list , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.
Nature 406:959-964(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U19797 Genomic DNA. Translation: AAF26456.1.
AE004091 Genomic DNA. Translation: AAG07798.1.
PIRC83094.
RefSeqNP_253100.1. NC_002516.2.

3D structure databases

ProteinModelPortalQ9LCT6.
SMRQ9LCT6. Positions 20-311.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING208964.PA4410.

Protocols and materials databases

DNASU881282.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAG07798; AAG07798; PA4410.
GeneID881282.
KEGGpae:PA4410.
PATRIC19843545. VBIPseAer58763_4619.

Organism-specific databases

PseudoCAPPA4410.

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011592.
KOK01921.
OMACANREEL.
OrthoDBEOG6ND0KB.
PhylomeDBQ9LCT6.

Enzyme and pathway databases

UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 2 hits.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDLB_PSEAE
AccessionPrimary (citable) accession number: Q9LCT6
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: October 1, 2000
Last modified: July 9, 2014
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways