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Q9LAP7

- AAGAR_ALTAG

UniProt

Q9LAP7 - AAGAR_ALTAG

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Protein
Alpha-agarase
Gene
agaA
Organism
Alteromonas agarilytica
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Alpha-agarase. Does not hydrolyze agarotetraose, agarohexaose, kappa-carrageenan, iota-carrageenan or lambda-carrageenan.1 Publication

Catalytic activityi

Endohydrolysis of 1,3-alpha-L-galactosidic linkages in agarose, yielding agarotetraose as the major product.1 Publication

Cofactori

Calcium.1 Publication

pH dependencei

Optimum pH is 7.2. Active between pH 6.0 and 9.0.1 Publication

Temperature dependencei

Inactive above 60 degrees Celsius.1 Publication

GO - Molecular functioni

  1. alpha-agarase activity Source: UniProtKB-EC
  2. calcium ion binding Source: InterPro
  3. carbohydrate binding Source: InterPro

GO - Biological processi

  1. cell adhesion Source: InterPro
  2. polysaccharide catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Polysaccharide degradation

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-16655.
BRENDAi3.2.1.158. 8321.

Protein family/group databases

CAZyiCBM6. Carbohydrate-Binding Module Family 6.
GH96. Glycoside Hydrolase Family 96.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-agarase (EC:3.2.1.158)
Gene namesi
Name:agaA
OrganismiAlteromonas agarilytica
Taxonomic identifieri105692 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesAlteromonadaceaeAlteromonas

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 26261 Publication
Add
BLAST
Chaini27 – 14291403Alpha-agarase1 Publication
PRO_5000055969Add
BLAST

Interactioni

Subunit structurei

Homodimer.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ9LAP7.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini29 – 161133CBM6 1
Add
BLAST
Domaini211 – 345135CBM6 2
Add
BLAST
Domaini662 – 793132CBM6 3
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Repeat, Signal

Family and domain databases

Gene3Di2.60.120.260. 3 hits.
4.10.1080.10. 2 hits.
InterProiIPR006584. Cellulose-bd_IV.
IPR029070. Chitinase_insertion.
IPR005084. CMB_fam6.
IPR008979. Galactose-bd-like.
IPR003367. Thrombospondin_3-like_rpt.
IPR028974. TSP_type-3_rpt.
[Graphical view]
PfamiPF03422. CBM_6. 3 hits.
PF02412. TSP_3. 5 hits.
[Graphical view]
SMARTiSM00606. CBD_IV. 1 hit.
[Graphical view]
SUPFAMiSSF103647. SSF103647. 3 hits.
SSF49785. SSF49785. 3 hits.
SSF54556. SSF54556. 1 hit.
PROSITEiPS51175. CBM6. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9LAP7-1 [UniParc]FASTAAdd to Basket

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MFKTKRSLLN SSIAISFAVL GVQAQAETLE LQAESFANSG GTYSDGQPNP     50
VTIYNVNGQG AINFVNAGDY VDYNINALGG EYDIEYFVGT GVTSGPNIEV 100
LVDVNGTWQS QGSVAVPYGS WDDFQSLTPS HTVTLPVGTS TVRLLAVGST 150
WQWNLESFRL TQVSPVEPVG DADNDGVNDN QDLCPNSPSG VTVDNNGCQI 200
TGGTDPGGES FVIQMEAFDS TGSDDSRAKG VIIGERGYPQ DKHTVVDSVQ 250
TTDWVDYSIN FPSSANYSVS MLASGQTDHA TAVLYLDGTE INEVPVHTGS 300
QADFANFQLA GSVYIASGTH TIRVQAQSST GEFSWLWFGD ALTFTNLDSD 350
GGNGGEATQD ADNDGVLDSS DSCPNTPTGE PADVTGCSAS QLDDDNDGVS 400
NNVDQCPNTV AGTEVDADGC EVIFADADND GIEDSQDFCP NTPAGEAVNN 450
SGCGASQLDA DNDGVTNNID QCPNTPAGTQ VDASGCETDN GGEPGDSYYH 500
NGQGLLFGRV DGATNFLGEE GYVANPDNYD VTTDLLETDD AIRANSTEVF 550
RGEIYDADGH IAFYEHIDDS VRLYIDGQLV LSNDSWENSS QTTDLNLTPG 600
WHNFELRLGN ADGGSGAVSG IGFGIDVDGG TNFVHPSNLS PSMFRASGQV 650
VVDPILPPSG GIYIQLEDFD ETGTVGRVAS DPNDGFVKGD SNVGWVTNGD 700
WGKYHNVFLE AGTYRAFITV STPAGGSYGA RVDIDGEPFA WGYFDSTGGW 750
DIAAEYELYG GHLVVESTGN HTLHVEAVGG SDWQWSGDLV RLAKVSDSAV 800
KQPRVYNPNE HIVAEIQGPA TGLQYLKTPV EIPLANKVLK SDVWYTYPQN 850
RNLVVDGDTP YADFGATGAF WGHPPEHDFY DDTVIMDWAV NVVDDFQSEG 900
FEYTARGEFD WGYGWFTEFT TNPQPHYVQT LDGRNVRMTF MGYLSHDGYN 950
NNWLSNHSPA FVPFMKSQVD QILKANPDKL MFDTQTNSTR STDMRTFGGD 1000
FSPYAMENFR VWLLKKYSNA QLVSMGINDI TSFDYGAYLR AQGITHTDWS 1050
NAGDTISGNI PMMEDFIYFN RDVWNQKFAE VLEYIRQQRP NIEIGASTHL 1100
FESRGYIFNE NITFLSGELN LGARTSISEL PTNILVHLKG AQAVDKTLAY 1150
FPYPWEFDEL RIQNAPRFGR GWVAQAYAYG GLFSIPANVW VGGEVFTWSP 1200
GADNYRDIYQ FVRAQANLLD GYTSYAKAGY VHAMFSSMKA GFIDGGNQVQ 1250
SSVKILTEDN INFDMLVFGD AGYPVVPRQA DFDKFEYIFY DGDLNYLTAE 1300
QQAVLDAQGS KVKHIGQRGT IAGLQINVSI NGSVSNETVS AVSRIHETDS 1350
TAPYVVHLIN RPFAGGVTPI LNNVEVAIPA SYFPQGVTSA KLHLPDGSSS 1400
TVAVSTNANG DTVVSVSNLE VWGILELAH 1429
Length:1,429
Mass (Da):154,671
Last modified:October 1, 2000 - v1
Checksum:i2ADD331171CBB5DC
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti42 – 432TY → FF AA sequence 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF121273 Genomic DNA. Translation: AAF26838.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF121273 Genomic DNA. Translation: AAF26838.1 .

3D structure databases

ProteinModelPortali Q9LAP7.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi CBM6. Carbohydrate-Binding Module Family 6.
GH96. Glycoside Hydrolase Family 96.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

BioCyci MetaCyc:MONOMER-16655.
BRENDAi 3.2.1.158. 8321.

Family and domain databases

Gene3Di 2.60.120.260. 3 hits.
4.10.1080.10. 2 hits.
InterProi IPR006584. Cellulose-bd_IV.
IPR029070. Chitinase_insertion.
IPR005084. CMB_fam6.
IPR008979. Galactose-bd-like.
IPR003367. Thrombospondin_3-like_rpt.
IPR028974. TSP_type-3_rpt.
[Graphical view ]
Pfami PF03422. CBM_6. 3 hits.
PF02412. TSP_3. 5 hits.
[Graphical view ]
SMARTi SM00606. CBD_IV. 1 hit.
[Graphical view ]
SUPFAMi SSF103647. SSF103647. 3 hits.
SSF49785. SSF49785. 3 hits.
SSF54556. SSF54556. 1 hit.
PROSITEi PS51175. CBM6. 3 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Alpha-agarases define a new family of glycoside hydrolases, distinct from beta-agarase families."
    Flament D., Barbeyron T., Jam M., Potin P., Czjzek M., Kloareg B., Michel G.
    Appl. Environ. Microbiol. 73:4691-4694(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 27-43 AND 802-821.
    Strain: GJ1B.
  2. "Purification and characterization of the alpha-agarase from Alteromonas agarlyticus (Cataldi) comb. nov., strain GJ1B."
    Potin P., Richard C., Rochas C., Kloareg B.
    Eur. J. Biochem. 214:599-607(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT.

Entry informationi

Entry nameiAAGAR_ALTAG
AccessioniPrimary (citable) accession number: Q9LAP7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: October 1, 2000
Last modified: June 11, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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