Q9LAP7 (AAGAR_ALTAG) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha-agarase
EC=3.2.1.158

Gene names

Name:

agaA

Organism

Alteromonas agarilytica

Taxonomic identifier

105692 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Alteromonadaceae › Alteromonas

Protein attributes

Sequence length	1429 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Alpha-agarase. Does not hydrolyze agarotetraose, agarohexaose, kappa-carrageenan, iota-carrageenen or lambda-carrageenan. Ref.2
Catalytic activity	Endohydrolysis of 1,3-alpha-L-galactosidic linkages in agarose, yielding agarotetraose as the major product. Ref.2
Cofactor	Calcium. Ref.2
Subunit structure	Homodimer. Ref.2
Sequence similarities	Belongs to the glycosyl hydrolase 96 family. Contains 3 CBM6 (carbohydrate binding type-6) domains.
Biophysicochemical properties	pH dependence: Optimum pH is 7.2. Active between pH 6.0 and 9.0. Ref.2 Temperature dependence: Inactive above 60 degrees Celsius. Ref.2

Ontologies

Keywords
Biological process	Carbohydrate metabolism Polysaccharide degradation
Domain	Repeat Signal
Ligand	Calcium Metal-binding
Molecular function	Glycosidase Hydrolase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	cell adhesion Inferred from electronic annotation. Source: InterPro polysaccharide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alpha-agarase activity Inferred from electronic annotation. Source: EC calcium ion binding Inferred from electronic annotation. Source: InterPro carbohydrate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 26	26	Ref.1
	Chain	27 – 1429	1403	Alpha-agarase Ref.1		PRO_5000055969
Regions
	Domain	29 – 161	133	CBM6 1
	Domain	211 – 345	135	CBM6 2
	Domain	662 – 793	132	CBM6 3
Experimental info
	Sequence conflict	42 – 43	2	TY → FF AA sequence Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9LAP7 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 2ADD331171CBB5DC	FASTA	1,429	154,671
	10 20 30 40 50 60 MFKTKRSLLN SSIAISFAVL GVQAQAETLE LQAESFANSG GTYSDGQPNP VTIYNVNGQG 70 80 90 100 110 120 AINFVNAGDY VDYNINALGG EYDIEYFVGT GVTSGPNIEV LVDVNGTWQS QGSVAVPYGS 130 140 150 160 170 180 WDDFQSLTPS HTVTLPVGTS TVRLLAVGST WQWNLESFRL TQVSPVEPVG DADNDGVNDN 190 200 210 220 230 240 QDLCPNSPSG VTVDNNGCQI TGGTDPGGES FVIQMEAFDS TGSDDSRAKG VIIGERGYPQ 250 260 270 280 290 300 DKHTVVDSVQ TTDWVDYSIN FPSSANYSVS MLASGQTDHA TAVLYLDGTE INEVPVHTGS 310 320 330 340 350 360 QADFANFQLA GSVYIASGTH TIRVQAQSST GEFSWLWFGD ALTFTNLDSD GGNGGEATQD 370 380 390 400 410 420 ADNDGVLDSS DSCPNTPTGE PADVTGCSAS QLDDDNDGVS NNVDQCPNTV AGTEVDADGC 430 440 450 460 470 480 EVIFADADND GIEDSQDFCP NTPAGEAVNN SGCGASQLDA DNDGVTNNID QCPNTPAGTQ 490 500 510 520 530 540 VDASGCETDN GGEPGDSYYH NGQGLLFGRV DGATNFLGEE GYVANPDNYD VTTDLLETDD 550 560 570 580 590 600 AIRANSTEVF RGEIYDADGH IAFYEHIDDS VRLYIDGQLV LSNDSWENSS QTTDLNLTPG 610 620 630 640 650 660 WHNFELRLGN ADGGSGAVSG IGFGIDVDGG TNFVHPSNLS PSMFRASGQV VVDPILPPSG 670 680 690 700 710 720 GIYIQLEDFD ETGTVGRVAS DPNDGFVKGD SNVGWVTNGD WGKYHNVFLE AGTYRAFITV 730 740 750 760 770 780 STPAGGSYGA RVDIDGEPFA WGYFDSTGGW DIAAEYELYG GHLVVESTGN HTLHVEAVGG 790 800 810 820 830 840 SDWQWSGDLV RLAKVSDSAV KQPRVYNPNE HIVAEIQGPA TGLQYLKTPV EIPLANKVLK 850 860 870 880 890 900 SDVWYTYPQN RNLVVDGDTP YADFGATGAF WGHPPEHDFY DDTVIMDWAV NVVDDFQSEG 910 920 930 940 950 960 FEYTARGEFD WGYGWFTEFT TNPQPHYVQT LDGRNVRMTF MGYLSHDGYN NNWLSNHSPA 970 980 990 1000 1010 1020 FVPFMKSQVD QILKANPDKL MFDTQTNSTR STDMRTFGGD FSPYAMENFR VWLLKKYSNA 1030 1040 1050 1060 1070 1080 QLVSMGINDI TSFDYGAYLR AQGITHTDWS NAGDTISGNI PMMEDFIYFN RDVWNQKFAE 1090 1100 1110 1120 1130 1140 VLEYIRQQRP NIEIGASTHL FESRGYIFNE NITFLSGELN LGARTSISEL PTNILVHLKG 1150 1160 1170 1180 1190 1200 AQAVDKTLAY FPYPWEFDEL RIQNAPRFGR GWVAQAYAYG GLFSIPANVW VGGEVFTWSP 1210 1220 1230 1240 1250 1260 GADNYRDIYQ FVRAQANLLD GYTSYAKAGY VHAMFSSMKA GFIDGGNQVQ SSVKILTEDN 1270 1280 1290 1300 1310 1320 INFDMLVFGD AGYPVVPRQA DFDKFEYIFY DGDLNYLTAE QQAVLDAQGS KVKHIGQRGT 1330 1340 1350 1360 1370 1380 IAGLQINVSI NGSVSNETVS AVSRIHETDS TAPYVVHLIN RPFAGGVTPI LNNVEVAIPA 1390 1400 1410 1420 SYFPQGVTSA KLHLPDGSSS TVAVSTNANG DTVVSVSNLE VWGILELAH

References

[1]	"Alpha-agarases define a new family of glycoside hydrolases, distinct from beta-agarase families." Flament D., Barbeyron T., Jam M., Potin P., Czjzek M., Kloareg B., Michel G. Appl. Environ. Microbiol. 73:4691-4694(2007) [PubMed: 17513582] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 27-43 AND 802-821. Strain: GJ1B.
[2]	"Purification and characterization of the alpha-agarase from Alteromonas agarlyticus (Cataldi) comb. nov., strain GJ1B." Potin P., Richard C., Rochas C., Kloareg B. Eur. J. Biochem. 214:599-607(1993) [PubMed: 8513809] [Abstract] Cited for: FUNCTION, CATALYTIC ACTIVITY, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF121273 Genomic DNA. Translation: AAF26838.1.
3D structure databases
ProteinModelPortal	Q9LAP7.
ModBase	Search...
Protein family/group databases
CAZy	CBM6. Carbohydrate-Binding Module Family 6. GH96. Glycoside Hydrolase Family 96.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-16655.
BRENDA	3.2.1.158. 8321.
Family and domain databases
InterPro	IPR006584. Cellulose-bd_IV. IPR005084. CMB_fam6. IPR008979. Galactose-bd-like. IPR003367. Thrombospondin_3-like_rpt. [Graphical view]
Pfam	PF03422. CBM_6. 3 hits. PF02412. TSP_3. 5 hits. [Graphical view]
SMART	SM00606. CBD_IV. 1 hit. [Graphical view]
SUPFAM	SSF49785. Gal_bind_like. 3 hits.
PROSITE	PS51175. CBM6. 3 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AAGAR_ALTAG

Accession

Primary (citable) accession number: Q9LAP7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 5, 2010
Last sequence update:	October 1, 2000
Last modified:	September 21, 2011
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program