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Q9LAP7

- AAGAR_ALTAG

UniProt

Q9LAP7 - AAGAR_ALTAG

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Protein

Alpha-agarase

Gene

agaA

Organism
Alteromonas agarilytica
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Alpha-agarase. Does not hydrolyze agarotetraose, agarohexaose, kappa-carrageenan, iota-carrageenan or lambda-carrageenan.1 Publication

Catalytic activityi

Endohydrolysis of 1,3-alpha-L-galactosidic linkages in agarose, yielding agarotetraose as the major product.1 Publication

Cofactori

Calcium.1 Publication

pH dependencei

Optimum pH is 7.2. Active between pH 6.0 and 9.0.1 Publication

Temperature dependencei

Inactive above 60 degrees Celsius.1 Publication

GO - Molecular functioni

  1. alpha-agarase activity Source: UniProtKB-EC
  2. calcium ion binding Source: InterPro
  3. carbohydrate binding Source: InterPro

GO - Biological processi

  1. cell adhesion Source: InterPro
  2. polysaccharide catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Polysaccharide degradation

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-16655.
BRENDAi3.2.1.158. 8321.

Protein family/group databases

CAZyiCBM6. Carbohydrate-Binding Module Family 6.
GH96. Glycoside Hydrolase Family 96.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-agaraseImported (EC:3.2.1.158)
Gene namesi
Name:agaAImported
OrganismiAlteromonas agarilytica
Taxonomic identifieri105692 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesAlteromonadaceaeAlteromonas

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 26261 PublicationAdd
BLAST
Chaini27 – 14291403Alpha-agarase1 PublicationPRO_5000055969Add
BLAST

Interactioni

Subunit structurei

Homodimer.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ9LAP7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini29 – 161133CBM6 1PROSITE-ProRule annotationAdd
BLAST
Domaini211 – 345135CBM6 2PROSITE-ProRule annotationAdd
BLAST
Domaini662 – 793132CBM6 3PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the glycosyl hydrolase 96 family.Sequence Analysis
Contains 3 CBM6 (carbohydrate binding type-6) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Family and domain databases

Gene3Di2.60.120.260. 3 hits.
4.10.1080.10. 2 hits.
InterProiIPR006584. Cellulose-bd_IV.
IPR029070. Chitinase_insertion.
IPR005084. CMB_fam6.
IPR008979. Galactose-bd-like.
IPR003367. Thrombospondin_3-like_rpt.
IPR028974. TSP_type-3_rpt.
[Graphical view]
PfamiPF03422. CBM_6. 3 hits.
PF02412. TSP_3. 5 hits.
[Graphical view]
SMARTiSM00606. CBD_IV. 1 hit.
[Graphical view]
SUPFAMiSSF103647. SSF103647. 3 hits.
SSF49785. SSF49785. 3 hits.
SSF54556. SSF54556. 1 hit.
PROSITEiPS51175. CBM6. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9LAP7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MFKTKRSLLN SSIAISFAVL GVQAQAETLE LQAESFANSG GTYSDGQPNP
60 70 80 90 100
VTIYNVNGQG AINFVNAGDY VDYNINALGG EYDIEYFVGT GVTSGPNIEV
110 120 130 140 150
LVDVNGTWQS QGSVAVPYGS WDDFQSLTPS HTVTLPVGTS TVRLLAVGST
160 170 180 190 200
WQWNLESFRL TQVSPVEPVG DADNDGVNDN QDLCPNSPSG VTVDNNGCQI
210 220 230 240 250
TGGTDPGGES FVIQMEAFDS TGSDDSRAKG VIIGERGYPQ DKHTVVDSVQ
260 270 280 290 300
TTDWVDYSIN FPSSANYSVS MLASGQTDHA TAVLYLDGTE INEVPVHTGS
310 320 330 340 350
QADFANFQLA GSVYIASGTH TIRVQAQSST GEFSWLWFGD ALTFTNLDSD
360 370 380 390 400
GGNGGEATQD ADNDGVLDSS DSCPNTPTGE PADVTGCSAS QLDDDNDGVS
410 420 430 440 450
NNVDQCPNTV AGTEVDADGC EVIFADADND GIEDSQDFCP NTPAGEAVNN
460 470 480 490 500
SGCGASQLDA DNDGVTNNID QCPNTPAGTQ VDASGCETDN GGEPGDSYYH
510 520 530 540 550
NGQGLLFGRV DGATNFLGEE GYVANPDNYD VTTDLLETDD AIRANSTEVF
560 570 580 590 600
RGEIYDADGH IAFYEHIDDS VRLYIDGQLV LSNDSWENSS QTTDLNLTPG
610 620 630 640 650
WHNFELRLGN ADGGSGAVSG IGFGIDVDGG TNFVHPSNLS PSMFRASGQV
660 670 680 690 700
VVDPILPPSG GIYIQLEDFD ETGTVGRVAS DPNDGFVKGD SNVGWVTNGD
710 720 730 740 750
WGKYHNVFLE AGTYRAFITV STPAGGSYGA RVDIDGEPFA WGYFDSTGGW
760 770 780 790 800
DIAAEYELYG GHLVVESTGN HTLHVEAVGG SDWQWSGDLV RLAKVSDSAV
810 820 830 840 850
KQPRVYNPNE HIVAEIQGPA TGLQYLKTPV EIPLANKVLK SDVWYTYPQN
860 870 880 890 900
RNLVVDGDTP YADFGATGAF WGHPPEHDFY DDTVIMDWAV NVVDDFQSEG
910 920 930 940 950
FEYTARGEFD WGYGWFTEFT TNPQPHYVQT LDGRNVRMTF MGYLSHDGYN
960 970 980 990 1000
NNWLSNHSPA FVPFMKSQVD QILKANPDKL MFDTQTNSTR STDMRTFGGD
1010 1020 1030 1040 1050
FSPYAMENFR VWLLKKYSNA QLVSMGINDI TSFDYGAYLR AQGITHTDWS
1060 1070 1080 1090 1100
NAGDTISGNI PMMEDFIYFN RDVWNQKFAE VLEYIRQQRP NIEIGASTHL
1110 1120 1130 1140 1150
FESRGYIFNE NITFLSGELN LGARTSISEL PTNILVHLKG AQAVDKTLAY
1160 1170 1180 1190 1200
FPYPWEFDEL RIQNAPRFGR GWVAQAYAYG GLFSIPANVW VGGEVFTWSP
1210 1220 1230 1240 1250
GADNYRDIYQ FVRAQANLLD GYTSYAKAGY VHAMFSSMKA GFIDGGNQVQ
1260 1270 1280 1290 1300
SSVKILTEDN INFDMLVFGD AGYPVVPRQA DFDKFEYIFY DGDLNYLTAE
1310 1320 1330 1340 1350
QQAVLDAQGS KVKHIGQRGT IAGLQINVSI NGSVSNETVS AVSRIHETDS
1360 1370 1380 1390 1400
TAPYVVHLIN RPFAGGVTPI LNNVEVAIPA SYFPQGVTSA KLHLPDGSSS
1410 1420
TVAVSTNANG DTVVSVSNLE VWGILELAH
Length:1,429
Mass (Da):154,671
Last modified:October 1, 2000 - v1
Checksum:i2ADD331171CBB5DC
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti42 – 432TY → FF AA sequence (PubMed:17513582)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF121273 Genomic DNA. Translation: AAF26838.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF121273 Genomic DNA. Translation: AAF26838.1 .

3D structure databases

ProteinModelPortali Q9LAP7.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi CBM6. Carbohydrate-Binding Module Family 6.
GH96. Glycoside Hydrolase Family 96.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

BioCyci MetaCyc:MONOMER-16655.
BRENDAi 3.2.1.158. 8321.

Family and domain databases

Gene3Di 2.60.120.260. 3 hits.
4.10.1080.10. 2 hits.
InterProi IPR006584. Cellulose-bd_IV.
IPR029070. Chitinase_insertion.
IPR005084. CMB_fam6.
IPR008979. Galactose-bd-like.
IPR003367. Thrombospondin_3-like_rpt.
IPR028974. TSP_type-3_rpt.
[Graphical view ]
Pfami PF03422. CBM_6. 3 hits.
PF02412. TSP_3. 5 hits.
[Graphical view ]
SMARTi SM00606. CBD_IV. 1 hit.
[Graphical view ]
SUPFAMi SSF103647. SSF103647. 3 hits.
SSF49785. SSF49785. 3 hits.
SSF54556. SSF54556. 1 hit.
PROSITEi PS51175. CBM6. 3 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Alpha-agarases define a new family of glycoside hydrolases, distinct from beta-agarase families."
    Flament D., Barbeyron T., Jam M., Potin P., Czjzek M., Kloareg B., Michel G.
    Appl. Environ. Microbiol. 73:4691-4694(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 27-43 AND 802-821.
    Strain: GJ1BImported.
  2. "Purification and characterization of the alpha-agarase from Alteromonas agarlyticus (Cataldi) comb. nov., strain GJ1B."
    Potin P., Richard C., Rochas C., Kloareg B.
    Eur. J. Biochem. 214:599-607(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT.

Entry informationi

Entry nameiAAGAR_ALTAG
AccessioniPrimary (citable) accession number: Q9LAP7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: October 1, 2000
Last modified: October 1, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3