Reviewed, UniProtKB/Swiss-Prot Q9LAM9 (MSRAB_STRGC)

Names and origin

Protein names

Recommended name:
    Peptide methionine sulfoxide reductase msrA/msrB
Including the following 2 domains:
    1- Recommended name:
            Peptide methionine sulfoxide reductase msrA
                Short name=Protein-methionine-S-oxide reductase
              EC=1.8.4.11
        Alternative name(s):
            Peptide-methionine (S)-S-oxide reductase
              Short name=Peptide Met(O) reductase
    2- Recommended name:
            Peptide methionine sulfoxide reductase msrB
              EC=1.8.4.12
        Alternative name(s):
            Peptide-methionine (R)-S-oxide reductase

Gene names

Name:	msrAB
Synonyms:	msrA
Ordered Locus Names:	SGO_0278

Organism

Streptococcus gordonii (strain Challis / ATCC 35105 / CH1 / DL1 / V288) [Complete proteome] [HAMAP]

Taxonomic identifier

29390 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	311 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Has an important function as a repair enzyme for proteins that have been inactivated by oxidation. Catalyzes the reversible oxidation-reduction of methionine sulfoxide in proteins to methionine By similarity. Involved in protection against oxidative stress when the bacterium enters the host bloodstream and required for maximal growth under aerobic and anaerobic conditions.
Catalytic activity	Peptide-L-methionine + thioredoxin disulfide + H2O = peptide-L-methionine (S)-S-oxide + thioredoxin. HAMAP MF_01400 L-methionine + thioredoxin disulfide + H2O = L-methionine (S)-S-oxide + thioredoxin. HAMAP MF_01400 Peptide-L-methionine + thioredoxin disulfide + H2O = peptide-L-methionine (R)-S-oxide + thioredoxin.
Sequence similarities	In the N-terminal section; belongs to the msrA Met sulfoxide reductase family. In the C-terminal section; belongs to the msrB Met sulfoxide reductase family.

Ontologies

Keywords
Molecular function	Oxidoreductase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW protein modification process Inferred from electronic annotation. Source: HAMAP
Molecular function	peptide-methionine (R)-S-oxide reductase activity Inferred from electronic annotation. Source: EC peptide-methionine-(S)-S-oxide reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 311	311	Peptide methionine sulfoxide reductase msrA/msrB HAMAP MF_01400		PRO_0000138516
Regions
	Region	1 – 155	155	Peptide methionine sulfoxide reductase A HAMAP MF_01400
	Region	172 – 295	124	Peptide methionine sulfoxide reductase B HAMAP MF_01400
Sites
	Active site	10	1	By similarity
	Active site	284	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9LAM9-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 7D4B7CAF81DBE692	FASTA	311	35,672
	10 20 30 40 50 60 MAEIYLAGGC FWGLEEYFSR IEGVKKTTVG YANGQVESTN YQLIHQTDHA ETVHLIYDEK 70 80 90 100 110 120 RVSLREILLY YFRVIDPLSV NKQGNDVGRQ YRTGVYYTNQ ADKAVIEQVF AEQEKQLGQK 130 140 150 160 170 180 IAVELEPLRH YVLAEDYHQD YLKKNPGGYC HINVNDAYQP LVDPGQYEKP TDAELKEQLT 190 200 210 220 230 240 QEQYQVTQLS ATERPFHNAY NATFEEGIYV DVTTGEPLFF AGDKFESGCG WPSFSRPIAR 250 260 270 280 290 300 EVLRYYEDKS HGMERIEVRS RSGNAHLGHV FTDGPESAGG LRYCINSAAL RFIPKEKMEA 310 EGYAYLLQHM K

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A shift from oral to blood pH is a stimulus for adaptive gene expression of Streptococcus gordonii CH1 and induces protection against oxidative stress and enhanced bacterial growth by expression of msrA." Vriesema A.J.M., Dankert J., Zaat S.A.J. Infect. Immun. 68:1061-1068(2000) [PubMed: 10678908] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
[2]	"Genome-wide transcriptional changes in Streptococcus gordonii in response to competence signaling peptide." Vickerman M.M., Iobst S., Jesionowski A.M., Gill S.R. J. Bacteriol. 189:7799-7807(2007) [PubMed: 17720781] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF128264 Genomic DNA. Translation: AAF36477.1. CP000725 Genomic DNA. Translation: ABV11083.1.
RefSeq	YP_001449597.1.
3D structure databases
HSSP	HSSP built from PDB template 1L1D based on UniProtKB P14930.
ModBase	Search...
Genome annotation databases
GeneID	5599737.
GenomeReviews	Gene locus SGO_0278 in contig CP000725_GR.
KEGG	sgo:SGO_0278.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	Q9LAM9. DERVIYL.
Family and domain databases
HAMAP	MF_01400. Fused. [Tree] MF_01401. Fused. [Tree]
InterPro	IPR002579. Methionine_sulphoxide_MsrB. IPR002569. MsrA. [Graphical view]
Gene3D	G3DSA:3.30.1060.10. MsrA. 1 hit. G3DSA:2.170.150.20. MsrB. 1 hit.
Pfam	PF01625. PMSR. 1 hit. PF01641. SelR. 1 hit. [Graphical view]
ProDom	PD004057. DUF25. 1 hit. PD003489. PMSR. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00401. msrA. 1 hit. TIGR00357. MsrB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MSRAB_STRGC

Accession

Primary (citable) accession number: Q9LAM9
Secondary accession number(s): A8AUY7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 2, 2002
Last sequence update:	October 1, 2000
Last modified:	June 16, 2009
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)