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Protein
Submitted name:

Alpha-2,3-/2,8-sialyltransferase

Gene

cst-II

Organism
Campylobacter jejuni
Status
Unreviewed-Annotation score: Annotation score: 1 out of 5-Experimental evidence at protein leveli

Functioni

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei129 – 1291Important for catalytic activityCombined sources
Binding sitei156 – 1561SubstrateCombined sources
Binding sitei162 – 1621SubstrateCombined sources
Active sitei188 – 1881Proton donor/acceptorCombined sources

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

GlycosyltransferaseImported, Transferase

Protein family/group databases

CAZyiGT42. Glycosyltransferase Family 42.

Names & Taxonomyi

Protein namesi
Submitted name:
Alpha-2,3-/2,8-sialyltransferaseImported
Submitted name:
Alpha-2,3/8-sialyltransferaseImported
Gene namesi
Name:cst-IIImported
OrganismiCampylobacter jejuniImported
Taxonomic identifieri197 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter

Interactioni

Protein-protein interaction databases

STRINGi354242.Cjejjejuni_010100006050.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1RO7X-ray1.80A/B/C/D1-259[»]
1RO8X-ray2.05A/B1-259[»]
2DRJX-ray2.25A1-259[»]
2WQQX-ray2.25A1-291[»]
2X61X-ray1.95A/B1-258[»]
2X62X-ray2.20A/B1-259[»]
2X63X-ray2.00A2-259[»]
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

eggNOGiENOG4105I85. Bacteria.
ENOG4111TI4. LUCA.

Family and domain databases

InterProiIPR009251. A-2_3-sialyltransferase.
[Graphical view]
PfamiPF06002. CST-I. 1 hit.
[Graphical view]
SUPFAMiSSF102414. SSF102414. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9LAK3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKVIIAGNG PSLKEIDYSR LPNDFDVFRC NQFYFEDKYY LGKKCKAVFY
60 70 80 90 100
NPILFFEQYY TLKHLIQNQE YETELIMCSN YNQAHLENEN FVKTFYDYFP
110 120 130 140 150
DAHLGYDFFK QLKDFNAYFK FHEIYFNQRI TSGVYMCAVA IALGYKEIYL
160 170 180 190 200
SGIDFYQNGS SYAFDTKQKN LLKLAPNFKN DNSHYIGHSK NTDIKALEFL
210 220 230 240 250
EKTYKIKLYC LCPNSLLANF IELAPNLNSN FIIQEKNNYT KDILIPSSEA
260 270 280 290
YGKFSKNINF KKIKIKENIY YKLIKDLLRL PSDIKHYFKG K
Length:291
Mass (Da):34,545
Last modified:October 1, 2000 - v1
Checksum:iAB0741D5FCEDAE6A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF130984 Genomic DNA. Translation: AAF31771.1.
AF216647 Genomic DNA. Translation: AAL36462.1.
AY644679 Genomic DNA. Translation: AAV52039.1.
RefSeqiWP_002852706.1. NZ_CZHY01000012.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF130984 Genomic DNA. Translation: AAF31771.1.
AF216647 Genomic DNA. Translation: AAL36462.1.
AY644679 Genomic DNA. Translation: AAV52039.1.
RefSeqiWP_002852706.1. NZ_CZHY01000012.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1RO7X-ray1.80A/B/C/D1-259[»]
1RO8X-ray2.05A/B1-259[»]
2DRJX-ray2.25A1-259[»]
2WQQX-ray2.25A1-291[»]
2X61X-ray1.95A/B1-258[»]
2X62X-ray2.20A/B1-259[»]
2X63X-ray2.00A2-259[»]
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi354242.Cjejjejuni_010100006050.

Protein family/group databases

CAZyiGT42. Glycosyltransferase Family 42.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG4105I85. Bacteria.
ENOG4111TI4. LUCA.

Family and domain databases

InterProiIPR009251. A-2_3-sialyltransferase.
[Graphical view]
PfamiPF06002. CST-I. 1 hit.
[Graphical view]
SUPFAMiSSF102414. SSF102414. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Cloning and sequencing of the cst-II gene from Campylobacter jejuni ATCC 700297."
    Gilbert M., Michniewicz J., Wakarchuk W.W.
    Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: ATCC 700297Imported.
  2. "Biosynthesis of ganglioside mimics in Campylobacter jejuni OH4384. Identification of the glycosyltransferase genes, enzymatic synthesis of model compounds, and characterization of nanomole amounts by 600-mhz (1)h and (13)c NMR analysis."
    Gilbert M., Brisson J.R., Karwaski M.F., Michniewicz J., Cunningham A.M., Wu Y., Young N.M., Wakarchuk W.W.
    J. Biol. Chem. 275:3896-3906(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: OH4384Imported.
  3. "Structural analysis of the sialyltransferase CstII from Campylobacter jejuni in complex with a substrate analog."
    Chiu C.P., Watts A.G., Lairson L.L., Gilbert M., Lim D., Wakarchuk W.W., Withers S.G., Strynadka N.C.
    Nat. Struct. Mol. Biol. 11:163-170(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS) OF 1-259, ACTIVE SITE.
  4. "Antecedent infections in Fisher Syndrome: a common pathogenesis of molecular mimicry."
    Koga M., Gilbert M., Li J., Koike S., Takahashi M., Furukawa K., Hirata K., Yuki N.
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: CF 93-6Imported.
  5. "Identification of a sialate O-acetyltransferase from Campylobacter jejuni: demonstration of direct transfer to the C-9 position of terminalalpha-2, 8-linked sialic acid."
    Houliston R.S., Endtz H.P., Yuki N., Li J., Jarrell H.C., Koga M., van Belkum A., Karwaski M.F., Wakarchuk W.W., Gilbert M.
    J. Biol. Chem. 281:11480-11486(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: OH4384Imported.
  6. "The charged sialic acid exhibits its power again: a new high-throughput screening technology."
    Wang P.G.
    Nat. Methods 3:589-590(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.25 ANGSTROMS) OF 1-259.
  7. "NMR spectroscopic characterization of the sialyltransferase CstII from Campylobacter jejuni: histidine 188 is the general base."
    Chan P.H., Lairson L.L., Lee H.J., Wakarchuk W.W., Strynadka N.C., Withers S.G., McIntosh L.P.
    Biochemistry 48:11220-11230(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.25 ANGSTROMS).
  8. "Structural and kinetic analysis of substrate binding to the sialyltransferase Cst-II from Campylobacter jejuni."
    Lee H.J., Lairson L.L., Rich J.R., Lameignere E., Wakarchuk W.W., Withers S.G., Strynadka N.C.
    J. Biol. Chem. 286:35922-35932(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS) OF 1-258.

Entry informationi

Entry nameiQ9LAK3_CAMJU
AccessioniPrimary (citable) accession number: Q9LAK3
Entry historyi
Integrated into UniProtKB/TrEMBL: October 1, 2000
Last sequence update: October 1, 2000
Last modified: July 6, 2016
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.