Reviewed, UniProtKB/Swiss-Prot Q9LAI2 (MTB1_BACSQ)

Names and origin

Protein names

Recommended name:
    Modification methylase BslI
      Short name=M.BslI
    EC=2.1.1.113
Alternative name(s):
    N(4)- cytosine-specific methyltransferase BslI

Gene names

Name:

bslIM

Organism

Bacillus sp. (strain NEB-606)

Taxonomic identifier

114630 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	912 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Recognizes the double-stranded sequence 5'-CCN7GG-3', probably causes specific methylation on C-1 or C-2 on both strands, and protects the DNA from cleavage by the BslI endonuclease. Ref.1
Catalytic activity	S-adenosyl-L-methionine + DNA cytosine = S-adenosyl-L-homocysteine + DNA N(4)-methylcytosine.
Domain	The N-terminal region is required for methylase activity, but its exact role is not clear.
Sequence similarities	Belongs to the N(4)/N(6)-methyltransferase family. N(4) subfamily.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological process	DNA methylation on cytosine Inferred from electronic annotation. Source: InterPro DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro N-methyltransferase activity Inferred from electronic annotation. Source: InterPro site-specific DNA-methyltransferase (cytosine-N4-specific) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 912	912	Modification methylase BslI		PRO_0000087922

Sequences

	Sequence		Length	Mass (Da)
	Q9LAI2-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 8FFF7F1F86102C25	FASTA	912	107,439
	10 20 30 40 50 60 MNWIFNTLIQ FLEDLNIDPS VVSLIDEDAK KLEEQFPKAL KHPVVDEEIV YKILCEKYNL 70 80 90 100 110 120 NALNVKTISE TLNKEYKFGR NSKTALKKYL DYGKEEYLIQ FFNTLMLENN TYIDREYIES 130 140 150 160 170 180 VLAFCEPVSK EKIKNEFIKL WNEANEVNEY GKLKDYLLGI YSKLFSMGLE NLRLIEIYNS 190 200 210 220 230 240 NESLIKKVFK YESTIKELKE YCLSNQESIT AGLAIKMFNE KYMELMKKEY QQDAIALKLE 250 260 270 280 290 300 EHMNQLYVDN NINEYPYIFD RGNDILLLPT EEYDFVYFHI DQDFFNRFQD ENKFLDYVLS 310 320 330 340 350 360 SIKQIYRVLA NEKVFALKID NIYNNEKNLK WELYPKLTIY SEHFIQTKET ARFYKAYDIA 370 380 390 400 410 420 KDLLSKHEFR LLENDSEKNR ENILKEYFSG KISEDELFSL VHVNMKKEHF FEFLNRFKYV 430 440 450 460 470 480 HYGFTFNDCL VLDRVDKSFA NGELENVISN ATEILLIFYK FRADQRRIPC PSCGSLNISG 490 500 510 520 530 540 NSYPEINNRS WECKSPYCPD RSKSNRGKRY SKKSNYMQWG AIYPKSHDII PRELIKKWRR 550 560 570 580 590 600 DIIVINNEQE IFEMLVKYFS FTDEKLLFIN TNELPSVVTE RENRKVVILS QKLKEKAYTS 610 620 630 640 650 660 NVVVKESLEG EIEFFKNGLY LKNFTELYLP EDQRRVSPEI NNFLNSGGRL KLIQGDSYEV 670 680 690 700 710 720 LKSVEDNTFA AAVTSPPYYN AREYSQWPNL YLYFNDMYNI IKECFRTLKP GSVFLYNIAD 730 740 750 760 770 780 IVDNENIIVK SSMGNKRIPL GAYTIYFFQK AGFELLDNII WDKGEPQSNR QKNDGKFTPH 790 800 810 820 830 840 YQKPLNAYEH MFIFKKTGAP LTLSDDWQSK RGSWIKNIVP FQPVFKINSK GENILGHTAP 850 860 870 880 890 900 FPEDIPRFVA NVFTKHDNDI ILDPFSGSLT SAIASYKSNR IGLGIELSPD YVELSRDRAL 910 LEGVTTKILN FN

References

[1]	"Cloning, expression, and purification of a thermostable nonhomodimeric restriction enzyme, BslI." Hsieh P.-C., Xiao J.-P., O'Loane D., Xu S.-Y. J. Bacteriol. 182:949-955(2000) [PubMed: 10648519] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF135191 Genomic DNA. Translation: AAF32529.1.
3D structure databases
ModBase	Search...
Protein family/group databases
REBASE	3310. M.BslI.
Family and domain databases
InterPro	IPR002941. DNA_methylase_N4/N6. IPR001091. MeTrfase_CN4. IPR017985. MeTrfase_CN4_CS. [Graphical view]
Pfam	PF01555. N6_N4_Mtase. 1 hit. [Graphical view]
PRINTS	PR00508. S21N4MTFRASE.
PROSITE	PS00093. N4_MTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTB1_BACSQ

Accession

Primary (citable) accession number: Q9LAI2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 16, 2004
Last sequence update:	October 1, 2000
Last modified:	June 16, 2009
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)