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Protein

Type-2 restriction enzyme BslI subunit alpha

Gene

bslIRalpha

Organism
Bacillus sp. (strain NEB-606)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Recognizes the double-stranded sequence 5'-CCN7GG-3' and cleaves after N-7.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Cofactori

Zn2+Note: Binds 2 Zn2+ ions per subunit.

Temperature dependencei

Is one of the thermostable restriction enzymes that remain active after 20 to 30 cycles of thermal PCR cycling.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri36 – 5318C4-type 1Add
BLAST
Zinc fingeri63 – 8422C4-type 2Add
BLAST

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

REBASEi386. BslI.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme BslI subunit alpha (EC:3.1.21.4)
Short name:
R.BslIalpha
Alternative name(s):
Endonuclease BslI subunit alpha
Type II restriction enzyme BslI subunit alpha
Type IIT restriction enzyme BslI subunit alpha
Gene namesi
Name:bslIRalpha
OrganismiBacillus sp. (strain NEB-606)
Taxonomic identifieri114630 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Pathology & Biotechi

Biotechnological usei

Could be used to screen carcinogenic mutations in a restriction endonuclease-mediated selective PCR (or REMS-PCR) assay. In particular, could be used to detect the vast majority of mutations that occur at codons 12 or 13 of the Ras oncogenes that encode glycine (codons GGN) at those positions.

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi36 – 361C → A: 10% of wild-type activity. 1 Publication
Mutagenesisi39 – 391C → A: 10% of wild-type activity. 1 Publication
Mutagenesisi50 – 501C → A: Loss of activity. 1 Publication
Mutagenesisi53 – 531C → A: 10% of wild-type activity. 1 Publication
Mutagenesisi53 – 531C → R: Loss of activity. 1 Publication
Mutagenesisi63 – 631C → A: Loss of activity. 1 Publication
Mutagenesisi66 – 661C → A: 50% of wild-type activity. 1 Publication
Mutagenesisi79 – 791C → A: 50% of wild-type activity. 1 Publication
Mutagenesisi84 – 841C → A: 50% of wild-type activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 225225Type-2 restriction enzyme BslI subunit alphaPRO_0000077283Add
BLAST

Interactioni

Subunit structurei

Heterotetramer of two alpha and two beta subunits. The alpha subunit is believed to be responsible for DNA recognition, while the beta subunit is thought to mediate cleavage.

Family & Domainsi

Domaini

One of the zinc-binding motifs may participate in protein-DNA interactions and the other might mediate protein-protein interactions.

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri36 – 5318C4-type 1Add
BLAST
Zinc fingeri63 – 8422C4-type 2Add
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Sequencei

Sequence statusi: Complete.

Q9LAI1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MERQLKSIAY AFVANDIDVY IPDGESNCIV VTKLVCKDCG QYWHTSLSEC
60 70 80 90 100
YFCGTLNFYL YECNSCGKKY SLTSSSKSCD TDGCNGKLIK RCSNPECISR
110 120 130 140 150
TNEEIQRATD EQGGVFDLNS SFNVSLNHCV TCGSKENYYK TYRIYSYRTE
160 170 180 190 200
VEPNIEALRE FANNNKLNSD EDVIIIKHLV DNVIHYGYIP YSKLDETTEI
210 220
TTTFSRFSDL VSELFPVNVP PNVTE
Length:225
Mass (Da):25,605
Last modified:September 30, 2000 - v1
Checksum:i4969BF02325E12B2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF135191 Genomic DNA. Translation: AAF32530.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF135191 Genomic DNA. Translation: AAF32530.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

REBASEi386. BslI.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

ProtoNetiSearch...

Publicationsi

  1. "Cloning, expression, and purification of a thermostable nonhomodimeric restriction enzyme, BslI."
    Hsieh P.-C., Xiao J.-P., O'Loane D., Xu S.-Y.
    J. Bacteriol. 182:949-955(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF N-TERMINUS, CHARACTERIZATION, MUTAGENESIS OF CYS-53.
  2. "Glucocorticoid receptor-like Zn(Cys)4 motifs in BslI restriction endonuclease."
    Scheuring Vanamee E., Hsieh P.-C., Zhu Z., Yates D., Garman E., Xu S.-Y., Aggarwal A.K.
    J. Mol. Biol. 334:595-603(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: ZINC-BINDING, ABSORPTION SPECTROSCOPY, MUTAGENESIS OF CYSTEINE RESIDUES.

Entry informationi

Entry nameiT2BLA_BACSQ
AccessioniPrimary (citable) accession number: Q9LAI1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 15, 2004
Last sequence update: September 30, 2000
Last modified: November 25, 2014
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.