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Q9LAI0

- T2BLB_BACSQ

UniProt

Q9LAI0 - T2BLB_BACSQ

Protein

Type-2 restriction enzyme BslI subunit beta

Gene

bslIRbeta

Organism
Bacillus sp. (strain NEB-606)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 36 (01 Oct 2014)
      Sequence version 1 (01 Oct 2000)
      Previous versions | rss
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    Functioni

    Recognizes the double-stranded sequence 5'-CCN7GG-3' and cleaves after N-7.

    Catalytic activityi

    Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

    Cofactori

    Binds 1 zinc ion per subunit.

    Temperature dependencei

    Is one of the thermostable restriction enzymes that remain active after 20 to 30 cycles of thermal PCR cycling.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri62 – 8221CHC2-typeAdd
    BLAST

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    Restriction system

    Keywords - Ligandi

    Metal-binding, Zinc

    Protein family/group databases

    REBASEi386. BslI.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Type-2 restriction enzyme BslI subunit beta (EC:3.1.21.4)
    Short name:
    R.BslIbeta
    Alternative name(s):
    Endonuclease BslI subunit beta
    Type II restriction enzyme BslI subunit beta
    Type IIT restriction enzyme BslI subunit beta
    Gene namesi
    Name:bslIRbeta
    OrganismiBacillus sp. (strain NEB-606)
    Taxonomic identifieri114630 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

    Pathology & Biotechi

    Biotechnological usei

    Could be used to screen carcinogenic mutations in a restriction endonuclease-mediated selective PCR (or REMS-PCR) assay. In particular, could be used to detect the vast majority of mutations that occur at codons 12 or 13 of the Ras oncogenes that encode glycine (codons GGN) at those positions.

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi62 – 621C → K: Loss of activity. 1 Publication
    Mutagenesisi66 – 661H → K: 50% of wild-type activity. 1 Publication
    Mutagenesisi79 – 791C → K: 10% of wild-type activity. 1 Publication
    Mutagenesisi82 – 821C → K: 10% of wild-type activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 301301Type-2 restriction enzyme BslI subunit betaPRO_0000077284Add
    BLAST

    Interactioni

    Subunit structurei

    Heterotetramer of two alpha and two beta subunits. The alpha subunit is believed to be responsible for DNA recognition, while the beta subunit is thought to mediate cleavage.

    Family & Domainsi

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri62 – 8221CHC2-typeAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Sequencei

    Sequence statusi: Complete.

    Q9LAI0-1 [UniParc]FASTAAdd to Basket

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    MEQQKFPNPR IFEDIDATDF SKHNKKHVTE DFVAENFKDV GWRVYRPFND    50
    TGIDLIAKKF VCPDGHTKWN QNLTKEMTCS ECGKSLIEIT RFIQVKTREV 100
    KQVKTREAKG EKFFFGYTLK SKDFRTDPRH VFLLYSDFTM DFIILPMYDY 150
    LNLFYTNQSL GSTHFSTPSF RQGNNKLNGL SKDKNDNWVW SGVSFNEFVN 200
    EKGMDKLSCP IYDIELESYT KKIQELKFSL FYRYSPGRKN QVSAPTVEFI 250
    NNHFSIFISL PKEAIASKRK AHLESLRQDL PEDLKKSVNE GYLVKFKGVD 300
    L 301
    Length:301
    Mass (Da):35,272
    Last modified:October 1, 2000 - v1
    Checksum:i9EFAD4778F5E5A4C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF135191 Genomic DNA. Translation: AAF32531.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF135191 Genomic DNA. Translation: AAF32531.1 .

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 386. BslI.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    ProtoNeti Search...

    Publicationsi

    1. "Cloning, expression, and purification of a thermostable nonhomodimeric restriction enzyme, BslI."
      Hsieh P.-C., Xiao J.-P., O'Loane D., Xu S.-Y.
      J. Bacteriol. 182:949-955(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF N-TERMINUS, CHARACTERIZATION.
    2. "Glucocorticoid receptor-like Zn(Cys)4 motifs in BslI restriction endonuclease."
      Scheuring Vanamee E., Hsieh P.-C., Zhu Z., Yates D., Garman E., Xu S.-Y., Aggarwal A.K.
      J. Mol. Biol. 334:595-603(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: ZINC-BINDING, ABSORPTION SPECTROSCOPY, MUTAGENESIS OF CYS-62; HIS-66; CYS-79 AND CYS-82.
      Strain: NEB-606.

    Entry informationi

    Entry nameiT2BLB_BACSQ
    AccessioniPrimary (citable) accession number: Q9LAI0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 16, 2004
    Last sequence update: October 1, 2000
    Last modified: October 1, 2014
    This is version 36 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries

    External Data

    Dasty 3