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Q9LAI0

- T2BLB_BACSQ

UniProt

Q9LAI0 - T2BLB_BACSQ

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Protein

Type-2 restriction enzyme BslI subunit beta

Gene

bslIRbeta

Organism
Bacillus sp. (strain NEB-606)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Recognizes the double-stranded sequence 5'-CCN7GG-3' and cleaves after N-7.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Cofactori

Binds 1 zinc ion per subunit.

Temperature dependencei

Is one of the thermostable restriction enzymes that remain active after 20 to 30 cycles of thermal PCR cycling.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri62 – 8221CHC2-typeAdd
BLAST

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

REBASEi386. BslI.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme BslI subunit beta (EC:3.1.21.4)
Short name:
R.BslIbeta
Alternative name(s):
Endonuclease BslI subunit beta
Type II restriction enzyme BslI subunit beta
Type IIT restriction enzyme BslI subunit beta
Gene namesi
Name:bslIRbeta
OrganismiBacillus sp. (strain NEB-606)
Taxonomic identifieri114630 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Pathology & Biotechi

Biotechnological usei

Could be used to screen carcinogenic mutations in a restriction endonuclease-mediated selective PCR (or REMS-PCR) assay. In particular, could be used to detect the vast majority of mutations that occur at codons 12 or 13 of the Ras oncogenes that encode glycine (codons GGN) at those positions.

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi62 – 621C → K: Loss of activity. 1 Publication
Mutagenesisi66 – 661H → K: 50% of wild-type activity. 1 Publication
Mutagenesisi79 – 791C → K: 10% of wild-type activity. 1 Publication
Mutagenesisi82 – 821C → K: 10% of wild-type activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 301301Type-2 restriction enzyme BslI subunit betaPRO_0000077284Add
BLAST

Interactioni

Subunit structurei

Heterotetramer of two alpha and two beta subunits. The alpha subunit is believed to be responsible for DNA recognition, while the beta subunit is thought to mediate cleavage.

Family & Domainsi

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri62 – 8221CHC2-typeAdd
BLAST

Keywords - Domaini

Zinc-finger

Sequencei

Sequence statusi: Complete.

Q9LAI0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEQQKFPNPR IFEDIDATDF SKHNKKHVTE DFVAENFKDV GWRVYRPFND
60 70 80 90 100
TGIDLIAKKF VCPDGHTKWN QNLTKEMTCS ECGKSLIEIT RFIQVKTREV
110 120 130 140 150
KQVKTREAKG EKFFFGYTLK SKDFRTDPRH VFLLYSDFTM DFIILPMYDY
160 170 180 190 200
LNLFYTNQSL GSTHFSTPSF RQGNNKLNGL SKDKNDNWVW SGVSFNEFVN
210 220 230 240 250
EKGMDKLSCP IYDIELESYT KKIQELKFSL FYRYSPGRKN QVSAPTVEFI
260 270 280 290 300
NNHFSIFISL PKEAIASKRK AHLESLRQDL PEDLKKSVNE GYLVKFKGVD

L
Length:301
Mass (Da):35,272
Last modified:October 1, 2000 - v1
Checksum:i9EFAD4778F5E5A4C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF135191 Genomic DNA. Translation: AAF32531.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF135191 Genomic DNA. Translation: AAF32531.1 .

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 386. BslI.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

ProtoNeti Search...

Publicationsi

  1. "Cloning, expression, and purification of a thermostable nonhomodimeric restriction enzyme, BslI."
    Hsieh P.-C., Xiao J.-P., O'Loane D., Xu S.-Y.
    J. Bacteriol. 182:949-955(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF N-TERMINUS, CHARACTERIZATION.
  2. "Glucocorticoid receptor-like Zn(Cys)4 motifs in BslI restriction endonuclease."
    Scheuring Vanamee E., Hsieh P.-C., Zhu Z., Yates D., Garman E., Xu S.-Y., Aggarwal A.K.
    J. Mol. Biol. 334:595-603(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: ZINC-BINDING, ABSORPTION SPECTROSCOPY, MUTAGENESIS OF CYS-62; HIS-66; CYS-79 AND CYS-82.
    Strain: NEB-606.

Entry informationi

Entry nameiT2BLB_BACSQ
AccessioniPrimary (citable) accession number: Q9LAI0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 16, 2004
Last sequence update: October 1, 2000
Last modified: October 1, 2014
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries

External Data

Dasty 3