##gff-version 3 Q9L9D7 UniProtKB Chain 1 574 . . . ID=PRO_0000090000;Note=Cocaine esterase Q9L9D7 UniProtKB Region 1 144 . . . Note=1A Q9L9D7 UniProtKB Region 145 240 . . . Note=2 Q9L9D7 UniProtKB Region 241 354 . . . Note=1B Q9L9D7 UniProtKB Region 355 574 . . . Note=3 Q9L9D7 UniProtKB Active site 117 117 . . . Note=Acyl-ester intermediate;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:11742345;Dbxref=PMID:11742345 Q9L9D7 UniProtKB Active site 259 259 . . . Note=Charge relay system;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:11742345;Dbxref=PMID:11742345 Q9L9D7 UniProtKB Active site 287 287 . . . Note=Charge relay system;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:11742345;Dbxref=PMID:11742345 Q9L9D7 UniProtKB Binding site 44 44 . . . . Q9L9D7 UniProtKB Binding site 118 118 . . . . Q9L9D7 UniProtKB Site 44 44 . . . Note=Probably involved in activating the substrate carbonyl and the acyl enzyme for hydrolysis Q9L9D7 UniProtKB Mutagenesis 44 44 . . . Note=Loss of activity. Has no protective effects against cocaine-induced convulsions and lethality in rat. Y->F;Ontology_term=ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:12369817,ECO:0000269|PubMed:16968810;Dbxref=PMID:12369817,PMID:16968810 Q9L9D7 UniProtKB Mutagenesis 55 55 . . . Note=Decrease in activity. Q->A%2CE;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:12369817;Dbxref=PMID:12369817 Q9L9D7 UniProtKB Mutagenesis 117 117 . . . Note=Loss of activity. Has no protective effects against cocaine-induced convulsions and lethality in rat. S->A;Ontology_term=ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:12369817,ECO:0000269|PubMed:16968810;Dbxref=PMID:12369817,PMID:16968810 Q9L9D7 UniProtKB Mutagenesis 117 117 . . . Note=Great decrease in activity. S->C;Ontology_term=ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:12369817,ECO:0000269|PubMed:16968810;Dbxref=PMID:12369817,PMID:16968810 Q9L9D7 UniProtKB Mutagenesis 151 151 . . . Note=Decrease in activity. W->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:12369817;Dbxref=PMID:12369817 Q9L9D7 UniProtKB Mutagenesis 166 166 . . . Note=Decrease in activity. W->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:12369817;Dbxref=PMID:12369817 Q9L9D7 UniProtKB Mutagenesis 169 169 . . . Note=Displays greatly enhanced stability%2C with a half-life of 570 minutes at 37 degrees Celsius. Exhibits 4.5-fold reduction in catalytic efficiency. L->K;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:20436035;Dbxref=PMID:20436035 Q9L9D7 UniProtKB Mutagenesis 172 172 . . . Note=Displays enhanced stability%2C with a half-life of 78 minutes at 37 degrees Celsius%2C and exhibits 3-fold reduction in catalytic efficiency. Displays enhanced stability%2C with a half-life of 370 minutes at 37 degrees Celsius%2C and exhibits 3-fold reduction in catalytic efficiency%3B when associated with Q-173. T->R;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:20436035;Dbxref=PMID:20436035 Q9L9D7 UniProtKB Mutagenesis 173 173 . . . Note=Displays enhanced stability%2C with a half-life of 75 minutes at 37 degrees Celsius%2C and has no deleterious effect on catalytic efficiency. Displays enhanced stability%2C with a half-life of 370 minutes at 37 degrees Celsius%2C and exhibits 3-fold reduction in catalytic efficiency%3B when associated with R-172. G->Q;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:20436035;Dbxref=PMID:20436035 Q9L9D7 UniProtKB Mutagenesis 259 259 . . . Note=Loss of activity. D->N;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:12369817;Dbxref=PMID:12369817 Q9L9D7 UniProtKB Mutagenesis 261 261 . . . Note=Decrease in activity. F->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:12369817;Dbxref=PMID:12369817 Q9L9D7 UniProtKB Mutagenesis 287 287 . . . Note=Loss of activity. H->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:12369817;Dbxref=PMID:12369817 Q9L9D7 UniProtKB Mutagenesis 407 407 . . . Note=Decrease in activity. L->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:12369817;Dbxref=PMID:12369817 Q9L9D7 UniProtKB Mutagenesis 408 408 . . . Note=Decrease in activity. F->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:12369817;Dbxref=PMID:12369817 Q9L9D7 UniProtKB Beta strand 6 15 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3I2K Q9L9D7 UniProtKB Beta strand 21 29 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3I2K Q9L9D7 UniProtKB Beta strand 35 44 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3I2K Q9L9D7 UniProtKB Helix 49 53 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3I2K Q9L9D7 UniProtKB Turn 54 56 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3I2K Q9L9D7 UniProtKB Helix 60 64 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3I2K Q9L9D7 UniProtKB Beta strand 68 73 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3I2K Q9L9D7 UniProtKB Turn 86 89 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3I2K Q9L9D7 UniProtKB Helix 90 103 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3I2K Q9L9D7 UniProtKB Beta strand 107 113 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3I2K Q9L9D7 UniProtKB Helix 118 127 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3I2K Q9L9D7 UniProtKB Beta strand 134 137 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3I2K Q9L9D7 UniProtKB Beta strand 139 141 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3I2K Q9L9D7 UniProtKB Helix 147 151 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3I2K Q9L9D7 UniProtKB Helix 160 177 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3I2K Q9L9D7 UniProtKB Beta strand 178 180 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3I2K Q9L9D7 UniProtKB Helix 185 196 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3I2K Q9L9D7 UniProtKB Helix 199 203 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3I2K Q9L9D7 UniProtKB Beta strand 205 207 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3PUI Q9L9D7 UniProtKB Helix 212 217 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3I2K Q9L9D7 UniProtKB Helix 220 223 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3I2K Q9L9D7 UniProtKB Turn 224 227 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3I2K Q9L9D7 UniProtKB Helix 233 236 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3I2K Q9L9D7 UniProtKB Helix 241 244 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3I2K Q9L9D7 UniProtKB Beta strand 251 258 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3I2K Q9L9D7 UniProtKB Helix 262 272 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3I2K Q9L9D7 UniProtKB Turn 273 275 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3I2K Q9L9D7 UniProtKB Beta strand 278 286 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3I2K Q9L9D7 UniProtKB Beta strand 291 294 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3I2K Q9L9D7 UniProtKB Helix 301 303 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3I2K Q9L9D7 UniProtKB Helix 307 322 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3I2K Q9L9D7 UniProtKB Turn 326 331 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3I2K Q9L9D7 UniProtKB Beta strand 334 340 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3I2K Q9L9D7 UniProtKB Turn 341 343 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3I2K Q9L9D7 UniProtKB Beta strand 344 352 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3I2K Q9L9D7 UniProtKB Beta strand 357 364 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3I2K Q9L9D7 UniProtKB Beta strand 377 381 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3I2K Q9L9D7 UniProtKB Beta strand 387 393 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3I2K Q9L9D7 UniProtKB Beta strand 407 410 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3I2K Q9L9D7 UniProtKB Helix 419 421 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3I2K Q9L9D7 UniProtKB Beta strand 428 431 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3I2K Q9L9D7 UniProtKB Beta strand 439 457 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3I2K Q9L9D7 UniProtKB Beta strand 459 467 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3I2K Q9L9D7 UniProtKB Beta strand 473 482 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3I2K Q9L9D7 UniProtKB Helix 483 485 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3I2K Q9L9D7 UniProtKB Beta strand 489 491 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3I2K Q9L9D7 UniProtKB Beta strand 501 514 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3I2K Q9L9D7 UniProtKB Beta strand 519 526 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3I2K Q9L9D7 UniProtKB Beta strand 537 540 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3I2K Q9L9D7 UniProtKB Helix 542 544 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3I2K Q9L9D7 UniProtKB Helix 547 549 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3I2K Q9L9D7 UniProtKB Beta strand 553 563 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3I2K Q9L9D7 UniProtKB Beta strand 566 572 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3I2K