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Protein

Malate dehydrogenase

Gene

mdh

Organism
Vibrio mimicus
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reversible oxidation of malate to oxaloacetate.By similarity

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei27NADBy similarity1
Binding sitei74SubstratePROSITE-ProRule annotation1
Binding sitei80SubstratePROSITE-ProRule annotation1
Binding sitei87NADBy similarity1
Binding sitei112SubstratePROSITE-ProRule annotation1
Binding sitei146SubstratePROSITE-ProRule annotation1
Active sitei170Proton acceptorBy similarity1
Binding sitei220NADBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi1 – 6NADBy similarity6
Nucleotide bindingi110 – 112NADBy similarity3

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

NAD

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenase (EC:1.1.1.37)
Gene namesi
Name:mdh
OrganismiVibrio mimicus
Taxonomic identifieri674 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000113331‹1 – ›222Malate dehydrogenaseAdd BLAST›222

Proteomic databases

PRIDEiQ9L8F6.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi675806.VII_003296.

Structurei

3D structure databases

ProteinModelPortaliQ9L8F6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 1 family.Curated

Phylogenomic databases

eggNOGiCOG0039. LUCA.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR010097. Malate_DH_type1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01772. MDH_euk_gproteo. 1 hit.
PROSITEiPS00068. MDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Fragment.

Q9L8F6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
AAGGIGQALA LLLKNRLPAG SDLALYDIAP VTPGVAADLS HIPTPVTIKG
60 70 80 90 100
YAGEDPTPAL EGADVVLVSA GVARKPGMDR ADLFNVNAGI VKALAETIAV
110 120 130 140 150
VCPKACVGII TNPVNTTVPI AAEVLKKAGV YDKRKLFGVT TLDVIRSETF
160 170 180 190 200
VAALKDKDPG QVRVPVIGGH SGVTILPLLS QVEGVTFTDE EVAALTKRIQ
210 220
NAGTEVVEAK AGGGSATFSM GQ
Length:222
Mass (Da):22,601
Last modified:October 1, 2000 - v1
Checksum:i593BDCAFBEAE464A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Non-terminal residuei11
Sequence conflicti211A → R in AAK15060 (PubMed:10986258).Curated1
Sequence conflicti211A → R in AAK15061 (PubMed:10986258).Curated1
Sequence conflicti211A → R in AAK15062 (PubMed:10986258).Curated1
Sequence conflicti218F → L in AAK15060 (PubMed:10986258).Curated1
Sequence conflicti218F → L in AAK15061 (PubMed:10986258).Curated1
Sequence conflicti218F → L in AAK15062 (PubMed:10986258).Curated1
Non-terminal residuei2221

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti194A → V in strain: 523-80. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF207856 Genomic DNA. Translation: AAF40141.1.
AF238335 Genomic DNA. Translation: AAK15060.1.
AF238336 Genomic DNA. Translation: AAK15061.1.
AF238337 Genomic DNA. Translation: AAK15062.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF207856 Genomic DNA. Translation: AAF40141.1.
AF238335 Genomic DNA. Translation: AAK15060.1.
AF238336 Genomic DNA. Translation: AAK15061.1.
AF238337 Genomic DNA. Translation: AAK15062.1.

3D structure databases

ProteinModelPortaliQ9L8F6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi675806.VII_003296.

Proteomic databases

PRIDEiQ9L8F6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiCOG0039. LUCA.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR010097. Malate_DH_type1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01772. MDH_euk_gproteo. 1 hit.
PROSITEiPS00068. MDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMDH_VIBMI
AccessioniPrimary (citable) accession number: Q9L8F6
Secondary accession number(s): Q99PX6, Q9AIU1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: October 1, 2000
Last modified: October 5, 2016
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.