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Protein

Malate dehydrogenase

Gene

mdh

Organism
Vibrio mimicus
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reversible oxidation of malate to oxaloacetate.By similarity

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei27 – 271NADBy similarity
Binding sitei74 – 741SubstratePROSITE-ProRule annotation
Binding sitei80 – 801SubstratePROSITE-ProRule annotation
Binding sitei87 – 871NADBy similarity
Binding sitei112 – 1121SubstratePROSITE-ProRule annotation
Binding sitei146 – 1461SubstratePROSITE-ProRule annotation
Active sitei170 – 1701Proton acceptorBy similarity
Binding sitei220 – 2201NADBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi1 – 66NADBy similarity
Nucleotide bindingi110 – 1123NADBy similarity

GO - Molecular functioni

  1. L-malate dehydrogenase activity Source: UniProtKB-EC

GO - Biological processi

  1. cellular carbohydrate metabolic process Source: InterPro
  2. malate metabolic process Source: InterPro
  3. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

NAD

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenase (EC:1.1.1.37)
Gene namesi
Name:mdh
OrganismiVibrio mimicus
Taxonomic identifieri674 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini‹1 – ›222›222Malate dehydrogenasePRO_0000113331Add
BLAST

Proteomic databases

PRIDEiQ9L8F6.

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ9L8F6.
SMRiQ9L8F6. Positions 1-222.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 1 family.Curated

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR010097. Malate_DH_type1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01772. MDH_euk_gproteo. 1 hit.
PROSITEiPS00068. MDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Fragment.

Q9L8F6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
AAGGIGQALA LLLKNRLPAG SDLALYDIAP VTPGVAADLS HIPTPVTIKG
60 70 80 90 100
YAGEDPTPAL EGADVVLVSA GVARKPGMDR ADLFNVNAGI VKALAETIAV
110 120 130 140 150
VCPKACVGII TNPVNTTVPI AAEVLKKAGV YDKRKLFGVT TLDVIRSETF
160 170 180 190 200
VAALKDKDPG QVRVPVIGGH SGVTILPLLS QVEGVTFTDE EVAALTKRIQ
210 220
NAGTEVVEAK AGGGSATFSM GQ
Length:222
Mass (Da):22,601
Last modified:October 1, 2000 - v1
Checksum:i593BDCAFBEAE464A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11
Sequence conflicti211 – 2111A → R in AAK15060 (PubMed:10986258).Curated
Sequence conflicti211 – 2111A → R in AAK15061 (PubMed:10986258).Curated
Sequence conflicti211 – 2111A → R in AAK15062 (PubMed:10986258).Curated
Sequence conflicti218 – 2181F → L in AAK15060 (PubMed:10986258).Curated
Sequence conflicti218 – 2181F → L in AAK15061 (PubMed:10986258).Curated
Sequence conflicti218 – 2181F → L in AAK15062 (PubMed:10986258).Curated
Non-terminal residuei222 – 2221

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti194 – 1941A → V in strain: 523-80.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF207856 Genomic DNA. Translation: AAF40141.1.
AF238335 Genomic DNA. Translation: AAK15060.1.
AF238336 Genomic DNA. Translation: AAK15061.1.
AF238337 Genomic DNA. Translation: AAK15062.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF207856 Genomic DNA. Translation: AAF40141.1.
AF238335 Genomic DNA. Translation: AAK15060.1.
AF238336 Genomic DNA. Translation: AAK15061.1.
AF238337 Genomic DNA. Translation: AAK15062.1.

3D structure databases

ProteinModelPortaliQ9L8F6.
SMRiQ9L8F6. Positions 1-222.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ9L8F6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR010097. Malate_DH_type1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01772. MDH_euk_gproteo. 1 hit.
PROSITEiPS00068. MDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Infectious CTXPhi and the Vibrio pathogenicity island prophage in Vibrio mimicus: evidence for recent horizontal transfer between V. mimicus and V. cholerae."
    Boyd E.F., Moyer K.E., Shi L., Waldor M.K.
    Infect. Immun. 68:1507-1513(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: PT5.
  2. "Molecular analyses of a putative CTXphi precursor and evidence for independent acquisition of distinct CTXphis by toxigenic Vibrio cholerae."
    Boyd E.F., Heilpern A.J., Waldor M.K.
    J. Bacteriol. 182:5530-5538(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 19-222.
    Strain: 523-80, PT48 and PT5.

Entry informationi

Entry nameiMDH_VIBMI
AccessioniPrimary (citable) accession number: Q9L8F6
Secondary accession number(s): Q99PX6, Q9AIU1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: October 1, 2000
Last modified: October 1, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.