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Q9L8F6 (MDH_VIBMI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Malate dehydrogenase
EC=1.1.1.37
Gene names
Name:mdh
OrganismVibrio mimicus
Taxonomic identifier674 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length222 AA.
Sequence statusFragment.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the reversible oxidation of malate to oxaloacetate By similarity. HAMAP-Rule MF_01516

Catalytic activity

(S)-malate + NAD+ = oxaloacetate + NADH. HAMAP-Rule MF_01516

Subunit structure

Homodimer By similarity.

Sequence similarities

Belongs to the LDH/MDH superfamily. MDH type 1 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain‹1 – ›222›222Malate dehydrogenase HAMAP-Rule MF_01516
PRO_0000113331

Regions

Nucleotide binding1 – 66NAD By similarity
Nucleotide binding110 – 1123NAD By similarity

Sites

Active site1701Proton acceptor By similarity
Binding site271NAD By similarity
Binding site741Substrate By similarity
Binding site801Substrate By similarity
Binding site871NAD By similarity
Binding site1121Substrate By similarity
Binding site1461Substrate By similarity
Binding site2201NAD By similarity

Natural variations

Natural variant1941A → V in strain: 523-80.

Experimental info

Sequence conflict2111A → R in AAK15060. Ref.2
Sequence conflict2111A → R in AAK15061. Ref.2
Sequence conflict2111A → R in AAK15062. Ref.2
Sequence conflict2181F → L in AAK15060. Ref.2
Sequence conflict2181F → L in AAK15061. Ref.2
Sequence conflict2181F → L in AAK15062. Ref.2
Non-terminal residue11
Non-terminal residue2221

Sequences

Sequence LengthMass (Da)Tools
Q9L8F6 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 593BDCAFBEAE464A

FASTA22222,601
        10         20         30         40         50         60 
AAGGIGQALA LLLKNRLPAG SDLALYDIAP VTPGVAADLS HIPTPVTIKG YAGEDPTPAL 

        70         80         90        100        110        120 
EGADVVLVSA GVARKPGMDR ADLFNVNAGI VKALAETIAV VCPKACVGII TNPVNTTVPI 

       130        140        150        160        170        180 
AAEVLKKAGV YDKRKLFGVT TLDVIRSETF VAALKDKDPG QVRVPVIGGH SGVTILPLLS 

       190        200        210        220 
QVEGVTFTDE EVAALTKRIQ NAGTEVVEAK AGGGSATFSM GQ

« Hide

References

[1]"Infectious CTXPhi and the Vibrio pathogenicity island prophage in Vibrio mimicus: evidence for recent horizontal transfer between V. mimicus and V. cholerae."
Boyd E.F., Moyer K.E., Shi L., Waldor M.K.
Infect. Immun. 68:1507-1513(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: PT5.
[2]"Molecular analyses of a putative CTXphi precursor and evidence for independent acquisition of distinct CTXphis by toxigenic Vibrio cholerae."
Boyd E.F., Heilpern A.J., Waldor M.K.
J. Bacteriol. 182:5530-5538(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 19-222.
Strain: 523-80, PT48 and PT5.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF207856 Genomic DNA. Translation: AAF40141.1.
AF238335 Genomic DNA. Translation: AAK15060.1.
AF238336 Genomic DNA. Translation: AAK15061.1.
AF238337 Genomic DNA. Translation: AAK15062.1.

3D structure databases

ProteinModelPortalQ9L8F6.
SMRQ9L8F6. Positions 1-222.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPMF_01516. Malate_dehydrog_1.
InterProIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR010097. Malate_DH_type1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERPTHR11540. PTHR11540. 1 hit.
PfamPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
SUPFAMSSF56327. Lactate_DH/Glyco_hydro_4_C. 1 hit.
TIGRFAMsTIGR01772. MDH_euk_gproteo. 1 hit.
PROSITEPS00068. MDH. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMDH_VIBMI
AccessionPrimary (citable) accession number: Q9L8F6
Secondary accession number(s): Q99PX6, Q9AIU1
Entry history
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: October 1, 2000
Last modified: April 3, 2013
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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