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Q9L8F6

- MDH_VIBMI

UniProt

Q9L8F6 - MDH_VIBMI

Protein

Malate dehydrogenase

Gene

mdh

Organism
Vibrio mimicus
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 67 (01 Oct 2014)
      Sequence version 1 (01 Oct 2000)
      Previous versions | rss
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    Functioni

    Catalyzes the reversible oxidation of malate to oxaloacetate.By similarity

    Catalytic activityi

    (S)-malate + NAD+ = oxaloacetate + NADH.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei27 – 271NADBy similarity
    Binding sitei74 – 741SubstratePROSITE-ProRule annotation
    Binding sitei80 – 801SubstratePROSITE-ProRule annotation
    Binding sitei87 – 871NADBy similarity
    Binding sitei112 – 1121SubstratePROSITE-ProRule annotation
    Binding sitei146 – 1461SubstratePROSITE-ProRule annotation
    Active sitei170 – 1701Proton acceptorBy similarity
    Binding sitei220 – 2201NADBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi1 – 66NADBy similarity
    Nucleotide bindingi110 – 1123NADBy similarity

    GO - Molecular functioni

    1. L-malate dehydrogenase activity Source: UniProtKB-EC

    GO - Biological processi

    1. cellular carbohydrate metabolic process Source: InterPro
    2. malate metabolic process Source: InterPro
    3. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Tricarboxylic acid cycle

    Keywords - Ligandi

    NAD

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Malate dehydrogenase (EC:1.1.1.37)
    Gene namesi
    Name:mdh
    OrganismiVibrio mimicus
    Taxonomic identifieri674 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini‹1 – ›222›222Malate dehydrogenasePRO_0000113331Add
    BLAST

    Proteomic databases

    PRIDEiQ9L8F6.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ9L8F6.
    SMRiQ9L8F6. Positions 1-222.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the LDH/MDH superfamily. MDH type 1 family.Curated

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    InterProiIPR001557. L-lactate/malate_DH.
    IPR022383. Lactate/malate_DH_C.
    IPR001236. Lactate/malate_DH_N.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR001252. Malate_DH_AS.
    IPR010097. Malate_DH_type1.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PANTHERiPTHR11540. PTHR11540. 1 hit.
    PfamiPF02866. Ldh_1_C. 1 hit.
    PF00056. Ldh_1_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF56327. SSF56327. 1 hit.
    TIGRFAMsiTIGR01772. MDH_euk_gproteo. 1 hit.
    PROSITEiPS00068. MDH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Fragment.

    Q9L8F6-1 [UniParc]FASTAAdd to Basket

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    AAGGIGQALA LLLKNRLPAG SDLALYDIAP VTPGVAADLS HIPTPVTIKG    50
    YAGEDPTPAL EGADVVLVSA GVARKPGMDR ADLFNVNAGI VKALAETIAV 100
    VCPKACVGII TNPVNTTVPI AAEVLKKAGV YDKRKLFGVT TLDVIRSETF 150
    VAALKDKDPG QVRVPVIGGH SGVTILPLLS QVEGVTFTDE EVAALTKRIQ 200
    NAGTEVVEAK AGGGSATFSM GQ 222
    Length:222
    Mass (Da):22,601
    Last modified:October 1, 2000 - v1
    Checksum:i593BDCAFBEAE464A
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Non-terminal residuei1 – 11
    Sequence conflicti211 – 2111A → R in AAK15060. (PubMed:10986258)Curated
    Sequence conflicti211 – 2111A → R in AAK15061. (PubMed:10986258)Curated
    Sequence conflicti211 – 2111A → R in AAK15062. (PubMed:10986258)Curated
    Sequence conflicti218 – 2181F → L in AAK15060. (PubMed:10986258)Curated
    Sequence conflicti218 – 2181F → L in AAK15061. (PubMed:10986258)Curated
    Sequence conflicti218 – 2181F → L in AAK15062. (PubMed:10986258)Curated
    Non-terminal residuei222 – 2221

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti194 – 1941A → V in strain: 523-80.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF207856 Genomic DNA. Translation: AAF40141.1.
    AF238335 Genomic DNA. Translation: AAK15060.1.
    AF238336 Genomic DNA. Translation: AAK15061.1.
    AF238337 Genomic DNA. Translation: AAK15062.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF207856 Genomic DNA. Translation: AAF40141.1 .
    AF238335 Genomic DNA. Translation: AAK15060.1 .
    AF238336 Genomic DNA. Translation: AAK15061.1 .
    AF238337 Genomic DNA. Translation: AAK15062.1 .

    3D structure databases

    ProteinModelPortali Q9L8F6.
    SMRi Q9L8F6. Positions 1-222.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi Q9L8F6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    InterProi IPR001557. L-lactate/malate_DH.
    IPR022383. Lactate/malate_DH_C.
    IPR001236. Lactate/malate_DH_N.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR001252. Malate_DH_AS.
    IPR010097. Malate_DH_type1.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    PANTHERi PTHR11540. PTHR11540. 1 hit.
    Pfami PF02866. Ldh_1_C. 1 hit.
    PF00056. Ldh_1_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56327. SSF56327. 1 hit.
    TIGRFAMsi TIGR01772. MDH_euk_gproteo. 1 hit.
    PROSITEi PS00068. MDH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Infectious CTXPhi and the Vibrio pathogenicity island prophage in Vibrio mimicus: evidence for recent horizontal transfer between V. mimicus and V. cholerae."
      Boyd E.F., Moyer K.E., Shi L., Waldor M.K.
      Infect. Immun. 68:1507-1513(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: PT5.
    2. "Molecular analyses of a putative CTXphi precursor and evidence for independent acquisition of distinct CTXphis by toxigenic Vibrio cholerae."
      Boyd E.F., Heilpern A.J., Waldor M.K.
      J. Bacteriol. 182:5530-5538(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 19-222.
      Strain: 523-80, PT48 and PT5.

    Entry informationi

    Entry nameiMDH_VIBMI
    AccessioniPrimary (citable) accession number: Q9L8F6
    Secondary accession number(s): Q99PX6, Q9AIU1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 7, 2004
    Last sequence update: October 1, 2000
    Last modified: October 1, 2014
    This is version 67 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3