Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein
Submitted name:

Endo-1,3-beta-glucanase

Gene
N/A
Organism
Streptomyces sioyaensis
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi64 – 641MagnesiumCombined sources
Metal bindingi114 – 1141Magnesium; via carbonyl oxygenCombined sources
Metal bindingi317 – 3171MagnesiumCombined sources

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

MagnesiumCombined sources, Metal-bindingCombined sources

Enzyme and pathway databases

BRENDAi3.2.1.39. 7530.

Protein family/group databases

CAZyiCBM6. Carbohydrate-Binding Module Family 6.
GH16. Glycoside Hydrolase Family 16.

Names & Taxonomyi

Protein namesi
Submitted name:
Endo-1,3-beta-glucanaseImported
OrganismiStreptomyces sioyaensisImported
Taxonomic identifieri67364 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomyces

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 4848Sequence analysisAdd
BLAST
Chaini49 – 478430Sequence analysisPRO_5004329484Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi209 ↔ 217Combined sources
Disulfide bondi229 ↔ 234Combined sources

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3DGTX-ray1.50A49-321[»]
ProteinModelPortaliQ9L816.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9L816.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 327326GH16 (glycosyl hydrolase family 16)InterPro annotationAdd
BLAST
Domaini347 – 477131CBM6 (carbohydrate binding type-6)InterPro annotationAdd
BLAST

Keywords - Domaini

SignalSequence analysis

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
2.60.120.260. 1 hit.
InterProiIPR006584. Cellulose-bd_IV.
IPR005084. CMB_fam6.
IPR013320. ConA-like_dom.
IPR008979. Galactose-bd-like.
IPR000757. GH16.
IPR006311. TAT_signal.
[Graphical view]
PfamiPF03422. CBM_6. 1 hit.
PF00722. Glyco_hydro_16. 1 hit.
[Graphical view]
SMARTiSM00606. CBD_IV. 1 hit.
[Graphical view]
SUPFAMiSSF49785. SSF49785. 1 hit.
SSF49899. SSF49899. 1 hit.
PROSITEiPS51175. CBM6. 1 hit.
PS51762. GH16_2. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9L816-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDSSGTPRP RSHSRPRSRS VRRALMAAVA TFGLAAAVAT AATGPADASA
60 70 80 90 100
PAPPSGWSQV FLDDFDGAAG SSVNTANWQF DTGTSYPGGA GNWGTGEVES
110 120 130 140 150
MTSSTSNVSL DGNGDLLITP RRDASGNWTS GRIETTRTDF QPPAGGKLRV
160 170 180 190 200
EARLQMPNVT GDAAAGYWPA FWMLGAPFRG NYQNWPGVGE LDIMENVQGL
210 220 230 240 250
NKTWATMHCG TSPGGPCNET SGIGNLTACP NTTCHSGLHT YTMEWDRSVS
260 270 280 290 300
PEAIRFSVDG VTYQTVTANH MDAVTWTNAT NHGFFVILNV AMGGGFPGAF
310 320 330 340 350
GGGPTGATEP GHPMVVDYVQ VLQSSGGGGG GGGGTTPPPT GDRDAYGQIQ
360 370 380 390 400
AESYDGQSGV ATETTTDTGG GQDMGYLANG DWALYKGVNF GSTPATQFYG
410 420 430 440 450
RVASGAGGGV SGLVEVRLDS RTNAPIGSFA VGDTGGWQSW RTVPANIGSV
460 470
TGTHDVYLTF SSGQPADFVN VNWFDFGH
Length:478
Mass (Da):49,432
Last modified:October 1, 2000 - v1
Checksum:iABAF0D89E7C255B3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF217415 Genomic DNA. Translation: AAF31438.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF217415 Genomic DNA. Translation: AAF31438.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3DGTX-ray1.50A49-321[»]
ProteinModelPortaliQ9L816.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiCBM6. Carbohydrate-Binding Module Family 6.
GH16. Glycoside Hydrolase Family 16.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BRENDAi3.2.1.39. 7530.

Miscellaneous databases

EvolutionaryTraceiQ9L816.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
2.60.120.260. 1 hit.
InterProiIPR006584. Cellulose-bd_IV.
IPR005084. CMB_fam6.
IPR013320. ConA-like_dom.
IPR008979. Galactose-bd-like.
IPR000757. GH16.
IPR006311. TAT_signal.
[Graphical view]
PfamiPF03422. CBM_6. 1 hit.
PF00722. Glyco_hydro_16. 1 hit.
[Graphical view]
SMARTiSM00606. CBD_IV. 1 hit.
[Graphical view]
SUPFAMiSSF49785. SSF49785. 1 hit.
SSF49899. SSF49899. 1 hit.
PROSITEiPS51175. CBM6. 1 hit.
PS51762. GH16_2. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Isolation and biochemical characterization of an endo-1,3-beta-glucanase from Streptomyces sioyaensis containing a C-terminal family 6 carbohydrate-binding module that binds to 1,3-beta-glucan."
    Hong T.-Y., Cheng C.-W., Huang J.-W., Meng M.
    Microbiology 148:1151-1159(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
  2. "The 1.5 A structure of endo-1,3-beta-glucanase from Streptomyces sioyaensis: evolution of the active-site structure for 1,3-beta-glucan-binding specificity and hydrolysis."
    Hong T.Y., Hsiao Y.Y., Meng M., Li T.T.
    Acta Crystallogr. D 64:964-970(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.50 ANGSTROMS) OF 49-321 IN COMPLEX WITH MAGNESIUM, DISULFIDE BONDS.

Entry informationi

Entry nameiQ9L816_9ACTN
AccessioniPrimary (citable) accession number: Q9L816
Entry historyi
Integrated into UniProtKB/TrEMBL: October 1, 2000
Last sequence update: October 1, 2000
Last modified: April 13, 2016
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.