Q9L7Q5 (GYRA_STRP1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA gyrase subunit A
EC=5.99.1.3

Gene names

Name:	gyrA
Ordered Locus Names:	SPy_1152, M5005_Spy0874

Organism

Streptococcus pyogenes serotype M1 [Complete proteome] [HAMAP]

Taxonomic identifier

301447 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	828 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings. HAMAP MF_01897
Catalytic activity	ATP-dependent breakage, passage and rejoining of double-stranded DNA. HAMAP MF_01897
Subunit structure	Made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer.
Subcellular location	Cytoplasm Potential HAMAP MF_01897.
Sequence similarities	Belongs to the topoisomerase GyrA/ParC subunit family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Isomerase Topoisomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 828	828	DNA gyrase subunit A HAMAP MF_01897		PRO_0000145264
Sites
	Active site	120	1	O-(5'-phospho-DNA)-tyrosine intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9L7Q5 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: A5FFBA83FD74A86A	FASTA	828	92,704
	10 20 30 40 50 60 MQDRNLIDVN LTSEMKTSFI DYAMSVIVAR ALPDVRDGLK PVHRRILYGM NELGVTPDKP 70 80 90 100 110 120 HKKSARITGD VMGKYHPHGD SSIYEAMVRM AQWWSYRHML VDGHGNFGSM DGDGAAAQRY 130 140 150 160 170 180 TEARMSKIAL ELLRDINKNT VNFQDNYDGS EREPVVLPAR FPNLLVNGAT GIAVGMATNI 190 200 210 220 230 240 PPHNLAESID AVKMVMEHPD CTTRELMEVI PGPDFPTGAL VMGRSGIHRA YDTGKGSIVL 250 260 270 280 290 300 RSRTEIETTQ TGRERIVVTE FPYGVNKTKV HEHIVRLAQE KRLEGITAVR DESSREGVRF 310 320 330 340 350 360 VIEIRREASA TVILNNLFKL TSLQTNFSFN MLAIENGVPK ILSLRQIIDN YISHQKEVII 370 380 390 400 410 420 RRTRFDKDKA EARAHILEGL LIALDHLDEV IAIIRNSETD VIAQTELMSR FDLSERQSQA 430 440 450 460 470 480 ILDMRLRRLT GLERDKIQSE YDDLLALIAD LSDILAKPER IITIIKEEMD EIKRKYANPR 490 500 510 520 530 540 RTELMVGEVL SLEDEDLIEE EDVLITLSNK GYIKRLAQDE FRAQKRGGRG VQGTGVNNDD 550 560 570 580 590 600 FVRELISTST HDTLLFFTNF GRVYRLKAYE IPEYGRTAKG LPIVNLLKLE DGETIQTIIN 610 620 630 640 650 660 ARKEETAGKS FFFTTKQGIV KRTEVSEFNN IRQNGLRALK LKEGDQLINV LLTSGQDDII 670 680 690 700 710 720 IGTHSGYSVR FNEASIRNMG RSATGVRGVK LREDDRVVGA SRIQDNQEVL VITENGFGKR 730 740 750 760 770 780 TSATDYPTKG RGGKGIKTAN ITPKNGQLAG LVTVDGTEDI MVITNKGVII RTNVANISQT 790 800 810 820 GRATLGVKIM KLDADAKIVT FTLVQPEDSS IAEINTDREN SISKNKDN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Resistance to multiple fluoroquinolone antibiotics in a clinical isolate of Streptococcus pyogenes: identification of gyrA and parC genes and point mutations responsible for the resistances." Yan S.S., Fedorko D.P., Gill V.J. Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 700294 / SF370 / Serotype M1.
[2]	"Complete genome sequence of an M1 strain of Streptococcus pyogenes." Ferretti J.J., McShan W.M., Ajdic D.J., Savic D.J., Savic G., Lyon K., Primeaux C., Sezate S., Suvorov A.N., Kenton S., Lai H.S., Lin S.P., Qian Y., Jia H.G., Najar F.Z., Ren Q., Zhu H., Song L. , White J., Yuan X., Clifton S.W., Roe B.A., McLaughlin R.E. Proc. Natl. Acad. Sci. U.S.A. 98:4658-4663(2001) [PubMed: 11296296] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700294 / SF370 / Serotype M1.
[3]	"Evolutionary origin and emergence of a highly successful clone of serotype M1 group A Streptococcus involved multiple horizontal gene transfer events." Sumby P., Porcella S.F., Madrigal A.G., Barbian K.D., Virtaneva K., Ricklefs S.M., Sturdevant D.E., Graham M.R., Vuopio-Varkila J., Hoe N.P., Musser J.M. J. Infect. Dis. 192:771-782(2005) [PubMed: 16088826] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-947 / MGAS5005 / Serotype M1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF220945 Genomic DNA. Translation: AAF63266.1. AE004092 Genomic DNA. Translation: AAK34024.1. CP000017 Genomic DNA. Translation: AAZ51492.1.
RefSeq	NP_269303.1. NC_002737.1. YP_282237.1. NC_007297.1.
3D structure databases
ProteinModelPortal	Q9L7Q5.
SMR	Q9L7Q5. Positions 31-484.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTRT00000001522; EBSTRP00000001522; EBSTRG00000001522. EBSTRT00000029015; EBSTRP00000028033; EBSTRG00000029015.
GeneID	3572044. 901268.
GenomeReviews	Gene locus SPy_1152 in contig AE004092_GR. Gene locus M5005_Spy0874 in contig CP000017_GR.
KEGG	spy:SPy_1152. spz:M5005_Spy_0874.
PATRIC	19715881. VBIStrPyo79812_1005.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000028052.
HOGENOM	HBG635183.
OMA	TGRGRIY.
ProtClustDB	PRK05560.
Enzyme and pathway databases
BioCyc	SPYO160490:SPY1152-MONOMER. SPYO293653:M5005_SPY0874-MONOMER.
Family and domain databases
HAMAP	MF_01897. GyrA. [Tree]
InterPro	IPR005743. GyrA. IPR006691. GyrA/parC_pinwhl. IPR002205. Topo_IIA_A/C. IPR013758. Topo_IIA_A/C_ab. IPR013757. Topo_IIA_A_a. IPR013760. Topo_IIA_cen. [Graphical view]
Gene3D	G3DSA:3.90.199.10. Topo_IIA_A/C_ab. 1 hit. G3DSA:1.10.268.10. Topo_IIA_A_a. 1 hit.
KO	K02469.
Pfam	PF03989. DNA_gyraseA_C. 6 hits. PF00521. DNA_topoisoIV. 1 hit. [Graphical view]
SMART	SM00434. TOP4c. 1 hit. [Graphical view]
SUPFAM	SSF56719. Topo_IIA_cen. 1 hit.
TIGRFAMs	TIGR01063. GyrA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GYRA_STRP1

Accession

Primary (citable) accession number: Q9L7Q5
Secondary accession number(s): Q48YT0

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2001
Last sequence update:	October 1, 2000
Last modified:	January 25, 2012
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program