Reviewed, UniProtKB/Swiss-Prot Q9L7P2 (ALGL_PSESY)

Names and origin

Protein names

Recommended name:
    Alginate lyase
    EC=4.2.2.3
Alternative name(s):
    Poly(beta-D-mannuronate) lyase
    Poly(mana) alginate lyase

Gene names

Name:

algL

Organism

Pseudomonas syringae pv. syringae

Taxonomic identifier

321 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas syringae

Protein attributes

Sequence length	378 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Depolymerizes alginate by cleaving the beta-1,4 glycosidic bond. Degrades deacetylated polymannuronate alginate from P.aeruginosa more efficiently than non-deacetylated polyM. AlgL from P.syringae degrades also its own alginate, which may indicate a role in cleaving preformed alginate and/or in determining the length of the alginate polymer. HAMAP MF_00557
Catalytic activity	Eliminative cleavage of polysaccharides containing beta-D-mannuronate residues to give oligosaccharides with 4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl groups at their ends. HAMAP MF_00557
Enzyme regulation	The monovalent cation sodium enhances activity but is not absolutely required. HAMAP MF_00557
Subcellular location	Periplasm HAMAP MF_00557.
Sequence similarities	Belongs to the polysaccharide lyase 5 family.
Biophysicochemical properties	pH dependence: Optimum pH is 7.0. HAMAP MF_00557 Temperature dependence: Optimum temperature is 42 degrees Celsius.

Ontologies

Keywords
Cellular component	Periplasm
Domain	Signal
Molecular function	Lyase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	alginic acid catabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	poly(beta-D-mannuronate) lyase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 28	28	Ref.1
	Chain	29 – 378	350	Alginate lyase HAMAP MF_00557		PRO_0000024922
Experimental info
	Mutagenesis	204	1	H → A: Loss of activity. Ref.1
	Mutagenesis	207	1	W → A: Loss of activity. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9L7P2-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 33E5894BC6404F14	FASTA	378	42,542
	10 20 30 40 50 60 MQTPKLIRPT LLSMAILSSM AWATGASAAL VPPKGYDAPI EKMKTGDHNF SCEAIPKPYT 70 80 90 100 110 120 DKLVFRSKYE GSDKARATLN AVSEEAFRDA TKDITTLERG VSKVVMQYMR DGRPEQLDCA 130 140 150 160 170 180 LNMMTTWAKA DALESREFNH TGKSMRKWAL GSMSSAYLRL KFSESHPLAN RQQDAKIIET 190 200 210 220 230 240 WFSKLADQVV SDWSNLPLEK INNHSYWAAW SVMATAVATN RQDLFDWAVK EYKVAANQVD 250 260 270 280 290 300 KDGFLPNEMK RRQRALSYHN YALPPLAMIA SFAQANGVDL RPENNGALKR LGDRVLAGVK 310 320 330 340 350 360 DPSIFAEHNG EKQDMTDLKK DPKFAWLEPY CSLYTCSPDV LEEKHEKQPF KTFRLGGDLT 370 KVYDPTHEKG DKGDNDGS

References

[1]

"Characterization of alginate lyase from Pseudomonas syringae pv. syringae." Preston L.A., Wong T.Y., Bender C.L., Schiller N.L. J. Bacteriol. 182:6268-6271(2000) [PubMed: 11029455] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 29-38, MUTAGENESIS OF HIS-204 AND TRP-207. Strain: FF5.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF222020 Genomic DNA. Translation: AAF32371.1.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	PL5. Polysaccharide Lyase Family 5.
Family and domain databases
HAMAP	MF_00557. Alginate_lyase. [Tree]
InterPro	IPR008397. Alginate_lyase. IPR008929. Chondroitin_lyas. [Graphical view]
Gene3D	G3DSA:1.50.10.110. Alginate_lyase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ALGL_PSESY

Accession

Primary (citable) accession number: Q9L7P2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 27, 2002
Last sequence update:	October 1, 2000
Last modified:	February 9, 2010
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)