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Protein

3-methyl-2-oxobutanoate hydroxymethyltransferase

Gene

panB

Organism
Mycobacterium vanbaalenii (strain DSM 7251 / PYR-1)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is transferred onto alpha-ketoisovalerate to form ketopantoate.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate + H2O = tetrahydrofolate + 2-dehydropantoate.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Pathwayi: (R)-pantothenate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes (R)-pantoate from 3-methyl-2-oxobutanoate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB)
  2. 2-dehydropantoate 2-reductase (Mvan_0557)
This subpathway is part of the pathway (R)-pantothenate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (R)-pantoate from 3-methyl-2-oxobutanoate, the pathway (R)-pantothenate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi61MagnesiumUniRule annotation1
Metal bindingi100MagnesiumUniRule annotation1
Binding sitei100Alpha-ketoisovalerateUniRule annotation1
Binding sitei130Alpha-ketoisovalerateUniRule annotation1
Metal bindingi132MagnesiumUniRule annotation1
Active sitei198Proton acceptorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processPantothenate biosynthesis
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciMVAN350058:G1G7S-3643-MONOMER
UniPathwayiUPA00028; UER00003

Names & Taxonomyi

Protein namesi
Recommended name:
3-methyl-2-oxobutanoate hydroxymethyltransferaseUniRule annotation (EC:2.1.2.11UniRule annotation)
Alternative name(s):
Ketopantoate hydroxymethyltransferaseUniRule annotation
Short name:
KPHMTUniRule annotation
Gene namesi
Name:panBUniRule annotation
Ordered Locus Names:Mvan_3602
OrganismiMycobacterium vanbaalenii (strain DSM 7251 / PYR-1)
Taxonomic identifieri350058 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium fortuitum complex
Proteomesi
  • UP000009159 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001848651 – 2803-methyl-2-oxobutanoate hydroxymethyltransferaseAdd BLAST280

Proteomic databases

PRIDEiQ9L7B2

Interactioni

Subunit structurei

Homodecamer; pentamer of dimers.UniRule annotation

Protein-protein interaction databases

STRINGi350058.Mvan_3602

Structurei

3D structure databases

ProteinModelPortaliQ9L7B2
SMRiQ9L7B2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni61 – 62Alpha-ketoisovalerate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the PanB family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CCG Bacteria
COG0413 LUCA
HOGENOMiHOG000078427
KOiK00606
OMAiGHIGLMP
OrthoDBiPOG091H02K8

Family and domain databases

CDDicd06557 KPHMT-like, 1 hit
HAMAPiMF_00156 PanB, 1 hit
InterProiView protein in InterPro
IPR003700 Pantoate_hydroxy_MeTrfase
IPR015813 Pyrv/PenolPyrv_Kinase-like_dom
PANTHERiPTHR20881 PTHR20881, 1 hit
PfamiView protein in Pfam
PF02548 Pantoate_transf, 1 hit
PIRSFiPIRSF000388 Pantoate_hydroxy_MeTrfase, 1 hit
SUPFAMiSSF51621 SSF51621, 1 hit
TIGRFAMsiTIGR00222 panB, 1 hit

Sequencei

Sequence statusi: Complete.

Q9L7B2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEQPVYGAA AEAPKPRVKV RTHHLHKWKA EGHKWAMLTA YDYSTAAVFD
60 70 80 90 100
EAEIPVLLVG DSAANVVYGY DTTVPVTLDE LIPLVRGVVR GAPHALVVAD
110 120 130 140 150
LPFGSYEAGP QQALATATRM LKETGAHAVK LEGGERVADQ IAAVSAAGIP
160 170 180 190 200
VMAHIGFTPQ SVNGLGGFRV QGRGDAAEQT IHDAIAVQEA GAFAVVMEMV
210 220 230 240 250
PAELATQITG KLTIPTVGIG AGPSCDAQVL VWQDMAGMTN GKTAKFVKRF
260 270 280
GAVGDELRRA ASDYAREVAT GAFPADEHSY
Length:280
Mass (Da):29,415
Last modified:October 1, 2000 - v1
Checksum:i624C2C837760150C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF223945 Genomic DNA Translation: AAF34708.1
CP000511 Genomic DNA Translation: ABM14394.1
PIRiT50554
RefSeqiWP_011780795.1, NC_008726.1

Genome annotation databases

EnsemblBacteriaiABM14394; ABM14394; Mvan_3602
KEGGimva:Mvan_3602

Similar proteinsi

Entry informationi

Entry nameiPANB_MYCVP
AccessioniPrimary (citable) accession number: Q9L7B2
Secondary accession number(s): A1TB44
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 23, 2003
Last sequence update: October 1, 2000
Last modified: May 23, 2018
This is version 97 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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