Reviewed, UniProtKB/Swiss-Prot Q9L772 (AQPZ_BRUME)

Names and origin

Protein names

Recommended name:
    Aquaporin Z

Gene names

Name:	aqpZ
Ordered Locus Names:	BMEI0070

Organism

Brucella melitensis [Complete proteome] [HAMAP]

Taxonomic identifier

29459 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	228 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Transport of water across the membrane. Possibly involved in the adaptation to variation in intravacuolar pH or osmolarity By similarity.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity.
Domain	Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA). HAMAP MF_01146
Sequence similarities	Belongs to the MIP/aquaporin (TC 1.A.8) family. [View classification]

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Repeat Transmembrane
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	transmembrane transport Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	water channel activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 228	228	Aquaporin Z HAMAP MF_01146		PRO_0000063985
Regions
	Topological domain	1 – 6	6	Cytoplasmic Potential
	Transmembrane	7 – 29	23	1 Potential
	Topological domain	30 – 33	4	Periplasmic Potential
	Transmembrane	34 – 56	23	2 Potential
	Topological domain	57 – 80	24	Cytoplasmic Potential
	Transmembrane	81 – 103	23	3 Potential
	Topological domain	104 – 126	23	Periplasmic Potential
	Transmembrane	127 – 149	23	4 Potential
	Topological domain	150 – 153	4	Cytoplasmic Potential
	Transmembrane	154 – 176	23	5 Potential
	Topological domain	177 – 202	26	Periplasmic Potential
	Transmembrane	203 – 225	23	6 Potential
	Topological domain	226 – 228	3	Cytoplasmic Potential
	Motif	63 – 65	3	NPA 1 HAMAP MF_01146
	Motif	184 – 186	3	NPA 2 HAMAP MF_01146
	Compositional bias	92 – 97	6	Poly-Ala HAMAP MF_01146
Sites
	Site	20	1	Involved in tetramerization or stability of the tetramer By similarity
	Site	43	1	Selectivity filter By similarity
	Site	172	1	Selectivity filter By similarity
	Site	181	1	Selectivity filter By similarity
	Site	187	1	Selectivity filter By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9L772-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 64282C39E4DD4109	FASTA	228	23,189
	10 20 30 40 50 60 MLNKLSAEFF GTFWLVFGGC GSAILAAAFP ELGIGFLGVA LAFGLTVLTM AYAVGGISGG 70 80 90 100 110 120 HFNPAVSLGL TVAGRLPAKD LIPYWVAQVL GAIAAAAILY VIASGKDGFS AGGLASNGYG 130 140 150 160 170 180 ELSPGGYSMM AGLLIEIILT AFFIIIILGS TSSLAPAGFA PIAIGFGLTL IHLVSIPVTN 190 200 210 220 TWVNPARSTG VALFADTAAL SQLWLFWVAP LVGAVIGAII WKGLLGRD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and nucleotide sequence of aquaporin gene of Brucella melitensis." Hernandez-Castro R., Verdugo-Rodriguez A., Gutierrez-Pabello J.A., Suarez-Guemes F. Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 133.
[2]	"The genome sequence of the facultative intracellular pathogen Brucella melitensis." DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. , Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., Haselkorn R., Kyrpides N.C., Overbeek R. Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed: 11756688] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094 / Biotype 1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF226624 Genomic DNA. Translation: AAF36396.1. AE008917 Genomic DNA. Translation: AAL51252.1.
PIR	AI3260.
RefSeq	NP_538988.1.
3D structure databases
SMR	Q9L772. Positions 1-207.
ModBase	Search...
Genome annotation databases
GeneID	1195782.
GenomeReviews	Gene locus BMEI0070 in contig AE008917_GR.
KEGG	bme:BMEI0070.
NMPDR	fig|224914.1.peg.70.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9L772.
OMA	LYTIASG
Enzyme and pathway databases
BioCyc	BMEL224914:BMEI0070-MON.
Family and domain databases
HAMAP	MF_01146. [Tree]
InterPro	IPR012269. Aquaporin. IPR000425. MIP. [Graphical view]
Gene3D	G3DSA:1.20.1080.10. MIP. 1 hit.
PANTHER	PTHR19139. MIP. 1 hit.
Pfam	PF00230. MIP. 1 hit. [Graphical view]
PRINTS	PR00783. MINTRINSICP.
TIGRFAMs	TIGR00861. MIP. 1 hit.
PROSITE	PS00221. MIP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AQPZ_BRUME

Accession

Primary (citable) accession number: Q9L772

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 5, 2001
Last sequence update:	October 1, 2000
Last modified:	November 24, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)