Q9L772 (AQPZ_BRUME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aquaporin Z

Gene names

Name:	aqpZ
Ordered Locus Names:	BMEI0070

Organism

Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094) [Complete proteome] [HAMAP]

Taxonomic identifier

224914 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	228 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Transport of water across the membrane. Possibly involved in the adaptation to variation in intravacuolar pH or osmolarity By similarity. HAMAP MF_01146
Subunit structure	Homotetramer By similarity. HAMAP MF_01146
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity HAMAP MF_01146.
Domain	Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA). HAMAP MF_01146
Sequence similarities	Belongs to the MIP/aquaporin (TC 1.A.8) family. [View classification]

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Repeat Transmembrane Transmembrane helix
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	water channel activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 228	228	Aquaporin Z HAMAP MF_01146		PRO_0000063985
Regions
	Topological domain	1 – 6	6	Cytoplasmic Potential
	Transmembrane	7 – 29	23	Helical; Name=1; Potential
	Topological domain	30 – 33	4	Periplasmic Potential
	Transmembrane	34 – 56	23	Helical; Name=2; Potential
	Topological domain	57 – 80	24	Cytoplasmic Potential
	Transmembrane	81 – 103	23	Helical; Name=3; Potential
	Topological domain	104 – 126	23	Periplasmic Potential
	Transmembrane	127 – 149	23	Helical; Name=4; Potential
	Topological domain	150 – 153	4	Cytoplasmic Potential
	Transmembrane	154 – 176	23	Helical; Name=5; Potential
	Topological domain	177 – 202	26	Periplasmic Potential
	Transmembrane	203 – 225	23	Helical; Name=6; Potential
	Topological domain	226 – 228	3	Cytoplasmic Potential
	Motif	63 – 65	3	NPA 1 HAMAP MF_01146
	Motif	184 – 186	3	NPA 2 HAMAP MF_01146
	Compositional bias	92 – 97	6	Poly-Ala HAMAP MF_01146
Sites
	Site	20	1	Involved in tetramerization or stability of the tetramer By similarity
	Site	43	1	Selectivity filter By similarity
	Site	172	1	Selectivity filter By similarity
	Site	181	1	Selectivity filter By similarity
	Site	187	1	Selectivity filter By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9L772 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 64282C39E4DD4109	FASTA	228	23,189
	10 20 30 40 50 60 MLNKLSAEFF GTFWLVFGGC GSAILAAAFP ELGIGFLGVA LAFGLTVLTM AYAVGGISGG 70 80 90 100 110 120 HFNPAVSLGL TVAGRLPAKD LIPYWVAQVL GAIAAAAILY VIASGKDGFS AGGLASNGYG 130 140 150 160 170 180 ELSPGGYSMM AGLLIEIILT AFFIIIILGS TSSLAPAGFA PIAIGFGLTL IHLVSIPVTN 190 200 210 220 TWVNPARSTG VALFADTAAL SQLWLFWVAP LVGAVIGAII WKGLLGRD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and nucleotide sequence of aquaporin gene of Brucella melitensis." Hernandez-Castro R., Verdugo-Rodriguez A., Gutierrez-Pabello J.A., Suarez-Guemes F. Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 133.
[2]	"The genome sequence of the facultative intracellular pathogen Brucella melitensis." DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. , Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., Haselkorn R., Kyrpides N.C., Overbeek R. Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed: 11756688] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF226624 Genomic DNA. Translation: AAF36396.1. AE008917 Genomic DNA. Translation: AAL51252.1.
PIR	AI3260.
RefSeq	NP_538988.1. NC_003317.1.
3D structure databases
ProteinModelPortal	Q9L772.
SMR	Q9L772. Positions 1-225.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1195782.
GenomeReviews	Gene locus BMEI0070 in contig AE008917_GR.
KEGG	bme:BMEI0070.
NMPDR	fig|224914.1.peg.70.
PATRIC	17795564. VBIBruMel146950_1055.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG705794.
OMA	PKGFAPI.
PhylomeDB	Q9L772.
ProtClustDB	PRK05420.
Enzyme and pathway databases
BioCyc	BMEL224914:BMEI0070-MONOMER.
Family and domain databases
HAMAP	MF_01146. Aquaporin_Z. [Tree]
InterPro	IPR012269. Aquaporin. IPR023271. Aquaporin-like. IPR023743. Aquaporin_Z. IPR000425. MIP. IPR022357. MIP_CS. [Graphical view]
Gene3D	G3DSA:1.20.1080.10. MIP. 1 hit.
KO	K06188.
PANTHER	PTHR19139. MIP. 1 hit.
Pfam	PF00230. MIP. 1 hit. [Graphical view]
PRINTS	PR00783. MINTRINSICP.
SUPFAM	SSF81338. MIP. 1 hit.
TIGRFAMs	TIGR00861. MIP. 1 hit.
PROSITE	PS00221. MIP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AQPZ_BRUME

Accession

Primary (citable) accession number: Q9L772

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 5, 2001
Last sequence update:	October 1, 2000
Last modified:	January 25, 2012
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program