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Q9L6B8 (PUR1_PASMU) Reviewed, UniProtKB/Swiss-Prot

Last modified November 13, 2013. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Amidophosphoribosyltransferase

Short name=ATase
EC=2.4.2.14
Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferase
Short name=GPATase
Gene names
Name:purF
Ordered Locus Names:PM0701
OrganismPasteurella multocida (strain Pm70) [Complete proteome] [HAMAP]
Taxonomic identifier272843 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaePasteurella

Protein attributes

Sequence length504 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.

Cofactor

Binds 1 magnesium ion per subunit.

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/2.

Sequence similarities

In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.

Contains 1 glutamine amidotransferase type-2 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 504503Amidophosphoribosyltransferase
PRO_0000139640

Regions

Domain2 – 235234Glutamine amidotransferase type-2

Sites

Active site21For GATase activity
Metal binding3671Magnesium By similarity
Metal binding3681Magnesium By similarity

Experimental info

Sequence conflict1181R → L in AAF68406. Ref.1
Sequence conflict4281K → N in AAF68406. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q9L6B8 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: BD19FE1E621D7A2E

FASTA50456,327
        10         20         30         40         50         60 
MCGIVGIVSQ SPVNQSIYDA LTLLQHRGQD AAGIVTVDDE NRFRLRKANG LVSDVFEQVH 

        70         80         90        100        110        120 
MLRLQGNAGI GHVRYPTAGS SSVSEAQPFY VNSPYGLTLV HNGNLTNSSE LKEKLFRRAR 

       130        140        150        160        170        180 
RHVNTNSDSE LLLNILANHL DHFEKYQLDP QDVFSAVKQT HQDIRGAYAC IAMIIGHGMV 

       190        200        210        220        230        240 
AFRDPNGIRP LVLGKREENG KTEYMFASES IALDTVGFEF VRDVQPGEAI YVTFEGEMYA 

       250        260        270        280        290        300 
QQCADKPTLT PCIFEYVYFA RPDSCIDGVS VYAARVHMGQ RLGEKIAREW ADVDDIDVVI 

       310        320        330        340        350        360 
PVPETSNDIA LRIARVLNKP YRQGFVKNRY VGRTFIMPGQ ALRVSSVRRK LNTIASEFKD 

       370        380        390        400        410        420 
KNVLLVDDSI VRGTTSEQIV EMARAAGAKK IYFASAAPEI RYPNVYGIDM PTKNELIAYG 

       430        440        450        460        470        480 
RDVDEIAKLI GVDKLIFQDL DALTGSVQQE NPSIQDFDCS VFTGVYVTGD ITPEYLDNIA 

       490        500 
EQRNDIAKKK REKDATNLEM HNEK 

« Hide

References

« Hide 'large scale' references
[1]"Identification of Pasteurella multocida virulence genes in a septicemic mouse model using signature-tagged mutagenesis."
Fuller T.E., Kennedy M.J., Lowery D.E.
Microb. Pathog. 29:25-38(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Complete genomic sequence of Pasteurella multocida Pm70."
May B.J., Zhang Q., Li L.L., Paustian M.L., Whittam T.S., Kapur V.
Proc. Natl. Acad. Sci. U.S.A. 98:3460-3465(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Pm70.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF237920 Genomic DNA. Translation: AAF68406.1.
AE004439 Genomic DNA. Translation: AAK02785.1.
RefSeqNP_245638.1. NC_002663.1.

3D structure databases

ProteinModelPortalQ9L6B8.
SMRQ9L6B8. Positions 2-477.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272843.PM0701.

Protein family/group databases

MEROPSC44.001.

Proteomic databases

PRIDEQ9L6B8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAK02785; AAK02785; PM0701.
GeneID1244048.
KEGGpmu:PM0701.
PATRIC22870596. VBIPasMul88067_0709.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0034.
HOGENOMHOG000033687.
KOK00764.
OMACDACFTG.
OrthoDBEOG6KT2Q1.
ProtClustDBPRK09246.

Enzyme and pathway databases

BioCycPMUL272843:GC8W-726-MONOMER.
UniPathwayUPA00074; UER00124.

Family and domain databases

InterProIPR005854. Amd_phspho_trans.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR000836. PRibTrfase_dom.
[Graphical view]
PfamPF00310. GATase_2. 2 hits.
PF00156. Pribosyltran. 1 hit.
[Graphical view]
PIRSFPIRSF000485. Amd_phspho_trans. 1 hit.
TIGRFAMsTIGR01134. purF. 1 hit.
PROSITEPS51278. GATASE_TYPE_2. 1 hit.
PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePUR1_PASMU
AccessionPrimary (citable) accession number: Q9L6B8
Entry history
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: January 23, 2007
Last modified: November 13, 2013
This is version 90 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways