Q9L6B8 (PUR1_PASMU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Amidophosphoribosyltransferase
Short name=ATase
EC=2.4.2.14

Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferase
Short name=GPATase

Gene names

Name:	purF
Ordered Locus Names:	PM0701

Organism

Pasteurella multocida (strain Pm70) [Complete proteome] [HAMAP]

Taxonomic identifier

272843 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Pasteurella › Pasteurella multocida › Pasteurella multocida subsp. multocida

Protein attributes

Sequence length	504 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.
Cofactor	Binds 1 magnesium ion per subunit.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/2.
Sequence similarities	In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family. Contains 1 glutamine amidotransferase type-2 domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Domain	Glutamine amidotransferase
Ligand	Magnesium Metal-binding
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW nucleoside metabolic process Inferred from electronic annotation. Source: InterPro purine nucleobase biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular_function	amidophosphoribosyltransferase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 504	503	Amidophosphoribosyltransferase		PRO_0000139640
Regions
	Domain	2 – 235	234	Glutamine amidotransferase type-2
Sites
	Active site	2	1	For GATase activity
	Metal binding	367	1	Magnesium By similarity
	Metal binding	368	1	Magnesium By similarity
Experimental info
	Sequence conflict	118	1	R → L in AAF68406. Ref.1
	Sequence conflict	428	1	K → N in AAF68406. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9L6B8 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: BD19FE1E621D7A2E	FASTA	504	56,327
	10 20 30 40 50 60 MCGIVGIVSQ SPVNQSIYDA LTLLQHRGQD AAGIVTVDDE NRFRLRKANG LVSDVFEQVH 70 80 90 100 110 120 MLRLQGNAGI GHVRYPTAGS SSVSEAQPFY VNSPYGLTLV HNGNLTNSSE LKEKLFRRAR 130 140 150 160 170 180 RHVNTNSDSE LLLNILANHL DHFEKYQLDP QDVFSAVKQT HQDIRGAYAC IAMIIGHGMV 190 200 210 220 230 240 AFRDPNGIRP LVLGKREENG KTEYMFASES IALDTVGFEF VRDVQPGEAI YVTFEGEMYA 250 260 270 280 290 300 QQCADKPTLT PCIFEYVYFA RPDSCIDGVS VYAARVHMGQ RLGEKIAREW ADVDDIDVVI 310 320 330 340 350 360 PVPETSNDIA LRIARVLNKP YRQGFVKNRY VGRTFIMPGQ ALRVSSVRRK LNTIASEFKD 370 380 390 400 410 420 KNVLLVDDSI VRGTTSEQIV EMARAAGAKK IYFASAAPEI RYPNVYGIDM PTKNELIAYG 430 440 450 460 470 480 RDVDEIAKLI GVDKLIFQDL DALTGSVQQE NPSIQDFDCS VFTGVYVTGD ITPEYLDNIA 490 500 EQRNDIAKKK REKDATNLEM HNEK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of Pasteurella multocida virulence genes in a septicemic mouse model using signature-tagged mutagenesis." Fuller T.E., Kennedy M.J., Lowery D.E. Microb. Pathog. 29:25-38(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete genomic sequence of Pasteurella multocida Pm70." May B.J., Zhang Q., Li L.L., Paustian M.L., Whittam T.S., Kapur V. Proc. Natl. Acad. Sci. U.S.A. 98:3460-3465(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Pm70.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF237920 Genomic DNA. Translation: AAF68406.1. AE004439 Genomic DNA. Translation: AAK02785.1.
RefSeq	NP_245638.1. NC_002663.1.
3D structure databases
ProteinModelPortal	Q9L6B8.
SMR	Q9L6B8. Positions 2-477.
ModBase	Search...
Protein-protein interaction databases
STRING	272843.PM0701.
Proteomic databases
PRIDE	Q9L6B8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAK02785; AAK02785; PM0701.
GeneID	1244048.
KEGG	pmu:PM0701.
PATRIC	22870596. VBIPasMul88067_0709.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0034.
HOGENOM	HOG000033687.
KO	K00764.
OMA	GIPFELG.
ProtClustDB	PRK09246.
Enzyme and pathway databases
UniPathway	UPA00074; UER00124.
Family and domain databases
InterPro	IPR005854. Amd_phspho_trans. IPR017932. GATase_2_dom. IPR000583. GATase_dom. IPR000836. PRibTrfase_dom. [Graphical view]
Pfam	PF00310. GATase_2. 2 hits. PF00156. Pribosyltran. 1 hit. [Graphical view]
PIRSF	PIRSF000485. Amd_phspho_trans. 1 hit.
TIGRFAMs	TIGR01134. purF. 1 hit.
PROSITE	PS51278. GATASE_TYPE_2. 1 hit. PS00103. PUR_PYR_PR_TRANSFER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR1_PASMU

Accession

Primary (citable) accession number: Q9L6B8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 87 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program