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Q9L6B8

- PUR1_PASMU

UniProt

Q9L6B8 - PUR1_PASMU

Protein

Amidophosphoribosyltransferase

Gene

purF

Organism
Pasteurella multocida (strain Pm70)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 93 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.

    Cofactori

    Binds 1 magnesium ion per subunit.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei2 – 21For GATase activity
    Metal bindingi367 – 3671MagnesiumBy similarity
    Metal bindingi368 – 3681MagnesiumBy similarity

    GO - Molecular functioni

    1. amidophosphoribosyltransferase activity Source: UniProtKB-EC
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
    2. glutamine metabolic process Source: UniProtKB-KW
    3. nucleoside metabolic process Source: InterPro
    4. purine nucleobase biosynthetic process Source: InterPro

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Purine biosynthesis

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciPMUL272843:GC8W-726-MONOMER.
    UniPathwayiUPA00074; UER00124.

    Protein family/group databases

    MEROPSiC44.001.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Amidophosphoribosyltransferase (EC:2.4.2.14)
    Short name:
    ATase
    Alternative name(s):
    Glutamine phosphoribosylpyrophosphate amidotransferase
    Short name:
    GPATase
    Gene namesi
    Name:purF
    Ordered Locus Names:PM0701
    OrganismiPasteurella multocida (strain Pm70)
    Taxonomic identifieri272843 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaePasteurella
    ProteomesiUP000000809: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 504503AmidophosphoribosyltransferasePRO_0000139640Add
    BLAST

    Proteomic databases

    PRIDEiQ9L6B8.

    Interactioni

    Protein-protein interaction databases

    STRINGi272843.PM0701.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9L6B8.
    SMRiQ9L6B8. Positions 2-477.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini2 – 235234Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.Curated
    Contains 1 glutamine amidotransferase type-2 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Glutamine amidotransferase

    Phylogenomic databases

    eggNOGiCOG0034.
    HOGENOMiHOG000033687.
    KOiK00764.
    OMAiAARVHMG.
    OrthoDBiEOG6KT2Q1.

    Family and domain databases

    Gene3Di3.40.50.2020. 1 hit.
    3.60.20.10. 1 hit.
    InterProiIPR005854. Amd_phspho_trans.
    IPR017932. GATase_2_dom.
    IPR000583. GATase_dom.
    IPR029055. Ntn_hydrolases_N.
    IPR000836. PRibTrfase_dom.
    IPR029057. PRTase-like.
    [Graphical view]
    PfamiPF00310. GATase_2. 2 hits.
    PF00156. Pribosyltran. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000485. Amd_phspho_trans. 1 hit.
    SUPFAMiSSF53271. SSF53271. 1 hit.
    SSF56235. SSF56235. 1 hit.
    TIGRFAMsiTIGR01134. purF. 1 hit.
    PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
    PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9L6B8-1 [UniParc]FASTAAdd to Basket

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    MCGIVGIVSQ SPVNQSIYDA LTLLQHRGQD AAGIVTVDDE NRFRLRKANG    50
    LVSDVFEQVH MLRLQGNAGI GHVRYPTAGS SSVSEAQPFY VNSPYGLTLV 100
    HNGNLTNSSE LKEKLFRRAR RHVNTNSDSE LLLNILANHL DHFEKYQLDP 150
    QDVFSAVKQT HQDIRGAYAC IAMIIGHGMV AFRDPNGIRP LVLGKREENG 200
    KTEYMFASES IALDTVGFEF VRDVQPGEAI YVTFEGEMYA QQCADKPTLT 250
    PCIFEYVYFA RPDSCIDGVS VYAARVHMGQ RLGEKIAREW ADVDDIDVVI 300
    PVPETSNDIA LRIARVLNKP YRQGFVKNRY VGRTFIMPGQ ALRVSSVRRK 350
    LNTIASEFKD KNVLLVDDSI VRGTTSEQIV EMARAAGAKK IYFASAAPEI 400
    RYPNVYGIDM PTKNELIAYG RDVDEIAKLI GVDKLIFQDL DALTGSVQQE 450
    NPSIQDFDCS VFTGVYVTGD ITPEYLDNIA EQRNDIAKKK REKDATNLEM 500
    HNEK 504
    Length:504
    Mass (Da):56,327
    Last modified:January 23, 2007 - v3
    Checksum:iBD19FE1E621D7A2E
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti118 – 1181R → L in AAF68406. (PubMed:10873488)Curated
    Sequence conflicti428 – 4281K → N in AAF68406. (PubMed:10873488)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF237920 Genomic DNA. Translation: AAF68406.1.
    AE004439 Genomic DNA. Translation: AAK02785.1.
    RefSeqiNP_245638.1. NC_002663.1.

    Genome annotation databases

    EnsemblBacteriaiAAK02785; AAK02785; PM0701.
    GeneIDi1244048.
    KEGGipmu:PM0701.
    PATRICi22870596. VBIPasMul88067_0709.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF237920 Genomic DNA. Translation: AAF68406.1 .
    AE004439 Genomic DNA. Translation: AAK02785.1 .
    RefSeqi NP_245638.1. NC_002663.1.

    3D structure databases

    ProteinModelPortali Q9L6B8.
    SMRi Q9L6B8. Positions 2-477.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 272843.PM0701.

    Protein family/group databases

    MEROPSi C44.001.

    Proteomic databases

    PRIDEi Q9L6B8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAK02785 ; AAK02785 ; PM0701 .
    GeneIDi 1244048.
    KEGGi pmu:PM0701.
    PATRICi 22870596. VBIPasMul88067_0709.

    Phylogenomic databases

    eggNOGi COG0034.
    HOGENOMi HOG000033687.
    KOi K00764.
    OMAi AARVHMG.
    OrthoDBi EOG6KT2Q1.

    Enzyme and pathway databases

    UniPathwayi UPA00074 ; UER00124 .
    BioCyci PMUL272843:GC8W-726-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.2020. 1 hit.
    3.60.20.10. 1 hit.
    InterProi IPR005854. Amd_phspho_trans.
    IPR017932. GATase_2_dom.
    IPR000583. GATase_dom.
    IPR029055. Ntn_hydrolases_N.
    IPR000836. PRibTrfase_dom.
    IPR029057. PRTase-like.
    [Graphical view ]
    Pfami PF00310. GATase_2. 2 hits.
    PF00156. Pribosyltran. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000485. Amd_phspho_trans. 1 hit.
    SUPFAMi SSF53271. SSF53271. 1 hit.
    SSF56235. SSF56235. 1 hit.
    TIGRFAMsi TIGR01134. purF. 1 hit.
    PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
    PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification of Pasteurella multocida virulence genes in a septicemic mouse model using signature-tagged mutagenesis."
      Fuller T.E., Kennedy M.J., Lowery D.E.
      Microb. Pathog. 29:25-38(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Pm70.

    Entry informationi

    Entry nameiPUR1_PASMU
    AccessioniPrimary (citable) accession number: Q9L6B8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 27, 2001
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 93 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3