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Protein

Amidophosphoribosyltransferase

Gene

purF

Organism
Pasteurella multocida (strain Pm70)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of phosphoribosylamine from phosphoribosylpyrophosphate (PRPP) and glutamine.UniRule annotation

Catalytic activityi

5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei2 – 21NucleophileUniRule annotation
Metal bindingi305 – 3051MagnesiumUniRule annotation
Metal bindingi367 – 3671MagnesiumUniRule annotation
Metal bindingi368 – 3681MagnesiumUniRule annotation

GO - Molecular functioni

  1. amidophosphoribosyltransferase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
  2. glutamine metabolic process Source: UniProtKB-KW
  3. nucleoside metabolic process Source: InterPro
  4. purine nucleobase biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciPMUL272843:GC8W-726-MONOMER.
UniPathwayiUPA00074; UER00124.

Names & Taxonomyi

Protein namesi
Recommended name:
AmidophosphoribosyltransferaseUniRule annotation (EC:2.4.2.14UniRule annotation)
Short name:
ATaseUniRule annotation
Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferaseUniRule annotation
Short name:
GPATaseUniRule annotation
Gene namesi
Name:purFUniRule annotation
Ordered Locus Names:PM0701
OrganismiPasteurella multocida (strain Pm70)
Taxonomic identifieri272843 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaePasteurella
ProteomesiUP000000809: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 504503AmidophosphoribosyltransferasePRO_0000139640Add
BLAST

Proteomic databases

PRIDEiQ9L6B8.

Interactioni

Protein-protein interaction databases

STRINGi272843.PM0701.

Structurei

3D structure databases

ProteinModelPortaliQ9L6B8.
SMRiQ9L6B8. Positions 2-477.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 235234Glutamine amidotransferase type-2UniRule annotationAdd
BLAST

Sequence similaritiesi

In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.UniRule annotation
Contains 1 glutamine amidotransferase type-2 domain.UniRule annotation

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiCOG0034.
HOGENOMiHOG000033687.
KOiK00764.
OMAiAARVHMG.
OrthoDBiEOG6KT2Q1.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
3.60.20.10. 1 hit.
HAMAPiMF_01931. PurF.
InterProiIPR005854. Amd_phspho_trans.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00310. GATase_2. 2 hits.
PF00156. Pribosyltran. 1 hit.
[Graphical view]
PIRSFiPIRSF000485. Amd_phspho_trans. 1 hit.
SUPFAMiSSF53271. SSF53271. 1 hit.
SSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01134. purF. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9L6B8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MCGIVGIVSQ SPVNQSIYDA LTLLQHRGQD AAGIVTVDDE NRFRLRKANG
60 70 80 90 100
LVSDVFEQVH MLRLQGNAGI GHVRYPTAGS SSVSEAQPFY VNSPYGLTLV
110 120 130 140 150
HNGNLTNSSE LKEKLFRRAR RHVNTNSDSE LLLNILANHL DHFEKYQLDP
160 170 180 190 200
QDVFSAVKQT HQDIRGAYAC IAMIIGHGMV AFRDPNGIRP LVLGKREENG
210 220 230 240 250
KTEYMFASES IALDTVGFEF VRDVQPGEAI YVTFEGEMYA QQCADKPTLT
260 270 280 290 300
PCIFEYVYFA RPDSCIDGVS VYAARVHMGQ RLGEKIAREW ADVDDIDVVI
310 320 330 340 350
PVPETSNDIA LRIARVLNKP YRQGFVKNRY VGRTFIMPGQ ALRVSSVRRK
360 370 380 390 400
LNTIASEFKD KNVLLVDDSI VRGTTSEQIV EMARAAGAKK IYFASAAPEI
410 420 430 440 450
RYPNVYGIDM PTKNELIAYG RDVDEIAKLI GVDKLIFQDL DALTGSVQQE
460 470 480 490 500
NPSIQDFDCS VFTGVYVTGD ITPEYLDNIA EQRNDIAKKK REKDATNLEM

HNEK
Length:504
Mass (Da):56,327
Last modified:January 23, 2007 - v3
Checksum:iBD19FE1E621D7A2E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti118 – 1181R → L in AAF68406. (PubMed:10873488)Curated
Sequence conflicti428 – 4281K → N in AAF68406. (PubMed:10873488)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF237920 Genomic DNA. Translation: AAF68406.1.
AE004439 Genomic DNA. Translation: AAK02785.1.
RefSeqiNP_245638.1. NC_002663.1.

Genome annotation databases

EnsemblBacteriaiAAK02785; AAK02785; PM0701.
GeneIDi1244048.
KEGGipmu:PM0701.
PATRICi22870596. VBIPasMul88067_0709.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF237920 Genomic DNA. Translation: AAF68406.1.
AE004439 Genomic DNA. Translation: AAK02785.1.
RefSeqiNP_245638.1. NC_002663.1.

3D structure databases

ProteinModelPortaliQ9L6B8.
SMRiQ9L6B8. Positions 2-477.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272843.PM0701.

Proteomic databases

PRIDEiQ9L6B8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK02785; AAK02785; PM0701.
GeneIDi1244048.
KEGGipmu:PM0701.
PATRICi22870596. VBIPasMul88067_0709.

Phylogenomic databases

eggNOGiCOG0034.
HOGENOMiHOG000033687.
KOiK00764.
OMAiAARVHMG.
OrthoDBiEOG6KT2Q1.

Enzyme and pathway databases

UniPathwayiUPA00074; UER00124.
BioCyciPMUL272843:GC8W-726-MONOMER.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
3.60.20.10. 1 hit.
HAMAPiMF_01931. PurF.
InterProiIPR005854. Amd_phspho_trans.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00310. GATase_2. 2 hits.
PF00156. Pribosyltran. 1 hit.
[Graphical view]
PIRSFiPIRSF000485. Amd_phspho_trans. 1 hit.
SUPFAMiSSF53271. SSF53271. 1 hit.
SSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01134. purF. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of Pasteurella multocida virulence genes in a septicemic mouse model using signature-tagged mutagenesis."
    Fuller T.E., Kennedy M.J., Lowery D.E.
    Microb. Pathog. 29:25-38(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Pm70.

Entry informationi

Entry nameiPUR1_PASMU
AccessioniPrimary (citable) accession number: Q9L6B8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: January 23, 2007
Last modified: January 7, 2015
This is version 96 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.